gurvan michel -...
TRANSCRIPT
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Discovery of novel enzymes for the valorization of algal biomass:
from genomes of marine bacteria to blue biotechnology
Gurvan Michel
Marine Glycobiology group, UMR 8227
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Me
-3OSO
OOSO
3-
O
O
OH
MeO
OSO3-
Polysaccharides
= ~50% algal biomass
Macroalgae: crucial role in the
coastal primary production
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Support the coastal food webs:
from marine herbivorous animals
to human activities
(sea vegetables, hydrocolloids)
Me
-3OSO
OOSO
3-
O
O
OH
MeO
OSO3-
Polysaccharides
= ~50% algal biomass
Macroalgae: crucial role in the
coastal primary production
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Marine heterotrophic bacteria:
key players in the recycling of algal biomass
Me
-3OSO
OOSO
3-
O
O
OH
MeO
OSO3-
Polysaccharides
= ~50% algal biomass
Macroalgae: crucial role in the
coastal primary production
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Laminarin
b(1-3) b(1-3)
O
O
O H
O
O H
H O
O
O O H
O H
H O
O
O O H
O H
H O
O
O
O H
O H
O
Me
-3OSO
OOSO
3-
O
O
OH
MeO
OSO3-
a-1,3
a-1,4
agarose
k-carrageenan
i-carrageenan
l-carrageenan
b(1-4) a(1-3) O
OO
O
OH
O
OH OH
OH
O
OOO
OSO3-
O
OH
OH
OH
O
OOO
OSO3-
O
OH
OH
OSO3-
O
OSO3-
O
OH
O
OSO3-
OH
OHO
OSO3-
Alginates
L-Guluronic acids D-Mannuronic acids
a-1,4 b-1,4
O
OO O
O
O
OHOH
OH
HOOC
HOOC
O
HOOC
OH
OHO
OH
HOOC
OH
OH
Algal polysaccharides: a underexploited renewable biomass
Fucans
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Laminarin
b(1-3) b(1-3)
O
O
O H
O
O H
H O
O
O O H
O H
H O
O
O O H
O H
H O
O
O
O H
O H
O
Me
-3OSO
OOSO
3-
O
O
OH
MeO
OSO3-
a-1,3
a-1,4
Fucans
agarose
k-carrageenan
i-carrageenan
l-carrageenan
b(1-4) a(1-3) O
OO
O
OH
O
OH OH
OH
O
OOO
OSO3-
O
OH
OH
OH
O
OOO
OSO3-
O
OH
OH
OSO3-
O
OSO3-
O
OH
O
OSO3-
OH
OHO
OSO3-
Huge chemical diversity
Algal sulfated polysaccharides have no equivalent in land plants
Diversity of the enzymes involved in their biosynthesis and their biodegradation
Algal polysaccharides: a underexploited renewable biomass
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Zobellia galactanivorans: a model marine bacterium for
algae-bacteria interactions
• Bacteroidetes isolated in Roscoff on a red alga
• Degrade most algal polysaccharides
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Genome sequencing (4738 genes, 5.5 MB)
• Large system for substrate detection and import
119 TonB-dependent receptors (TBDR) and 65 one/two-component systems
• Confirmation of the huge potential for polysaccharide degradation:
141 Glycoside hydrolases (GH) and 71 sulfatases !
• Bacteroidetes isolated in Roscoff on a red alga
• Degrade most algal polysaccharides
Zobellia galactanivorans: a model marine bacterium for
algae-bacteria interactions
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Genome sequencing (4738 genes, 5.5 MB)
• Large system for substrate detection and import
119 TonB-dependent receptors (TBDR) and 65 one/two-component systems
• Confirmation of the huge potential for polysaccharide degradation:
141 Glycoside hydrolases (GH) and 71 sulfatases !
• Numerous putative operons specific for polysaccharide utilization
• Bacteroidetes isolated in Roscoff on a red alga
• Degrade most algal polysaccharides
Zg2624 Zg2615 Zg2613 Zg2614 Zg2616 Zg2617 Zg2618 Zg2619 Zg2620 Zg2621 Zg2622 Zg2623
Sugar kinase
transcriptional
factor UNK
SusD-like
TBDR
Sugar
dehydrogenase
KgdF-like
PL17 PL7
Sugar
permease
Sugar
dehydrogenase Sugar kinase
Zg2612
NRAMP
transporter
Zobellia galactanivorans: a model marine bacterium for
algae-bacteria interactions
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Zg3628 AgaF
Zg3640 AgaG
Zg1017 AgaH
Zg3376 AgaI
Zg2600 AgaE
Zg4267 AgaC
Zg4203 AgaA
Zg3573 AgaB
Zg4243 AgaD
Zg236 CgkA
Zg2431 LamA
Zg367 LamB
Zg1010 LamC
Zg1014 LamD
Zg1021
Z3347
LicA Bl
74
100
96
63
59
95
67
48
46
33
74
37
38
41
0.5
Phylogenetic tree of the GH16 family from Zobellia
Allouch et al, (2003) JBC & ( 2004) Structure
Jam et al (2005) Biochem J
AgaB
AgaA
Barbeyron et al (1998)
Mol Biol Evol
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Zg3628 AgaF
Zg3640 AgaG
Zg1017 AgaH
Zg3376 AgaI
Zg2600 AgaE
Zg4267 AgaC
Zg4203 AgaA
Zg3573 AgaB
Zg4243 AgaD
Zg236 CgkA
Zg2431 LamA
Zg367 LamB
Zg1010 LamC
Zg1014 LamD
Zg1021
Z3347
LicA Bl
74
100
96
63
59
95
67
48
46
33
74
37
38
41
0.5
GH16 X70 UNK
GH16
GH16 CBM6
GH16 CBM16
b-agarases
k-carrageenases
Phylogenetic tree of the GH16 family from Zobellia
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Zg3628 AgaF
Zg3640 AgaG
Zg1017 AgaH
Zg3376 AgaI
Zg2600 AgaE
Zg4267 AgaC
Zg4203 AgaA
Zg3573 AgaB
Zg4243 AgaD
Zg236 CgkA
Zg2431 LamA
Zg367 LamB
Zg1010 LamC
Zg1014 LamD
Zg1021
Z3347
LicA Bl
74
100
96
63
59
95
67
48
46
33
74
37
38
41
0.5
Phylogenetic tree of the GH16 family from Zobellia
AgaD
Hehemann et al (2013) JBC
GH16 CBM6
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Zg3628 AgaF
Zg3640 AgaG
Zg1017 AgaH
Zg3376 AgaI
Zg2600 AgaE
Zg4267 AgaC
Zg4203 AgaA
Zg3573 AgaB
Zg4243 AgaD
Zg236 CgkA
Zg2431 LamA
Zg367 LamB
Zg1010 LamC
Zg1014 LamD
Zg1021
Z3347
LicA Bl
74
100
96
63
59
95
67
48
46
33
74
37
38
41
0.5
GH16 PKD
GH16
GH16 UNK CBM42
GH16 CBM6
Laminarinases
Phylogenetic tree of the GH16 family from Zobellia
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Zg3628 AgaF
Zg3640 AgaG
Zg1017 AgaH
Zg3376 AgaI
Zg2600 AgaE
Zg4267 AgaC
Zg4203 AgaA
Zg3573 AgaB
Zg4243 AgaD
Zg236 CgkA
Zg2431 LamA
Zg367 LamB
Zg1010 LamC
Zg1014 LamD
Zg1021
Z3347
LicA Bl
74
100
96
63
59
95
67
48
46
33
74
37
38
41
0.5
Phylogenetic tree of the GH16 family from Zobellia
GH16 PKD
GH16 CBM6
LamA_GH16
LamC_GH16
LamC_CBM6
Labourel et al (2014) JBC
Labourel et al (2015)
Acta Cryst D
Jam et al (2016)
FEBS Journal
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Zg3628 AgaF
Zg3640 AgaG
Zg1017 AgaH
Zg3376 AgaI
Zg2600 AgaE
Zg4267 AgaC
Zg4203 AgaA
Zg3573 AgaB
Zg4243 AgaD
Zg236 CgkA
Zg2431 LamA
Zg367 LamB
Zg1010 LamC
Zg1014 LamD
Zg1021
Z3347
LicA Bl
74
100
96
63
59
95
67
48
46
33
74
37
38
41
0.5
GH16
GH16 UNK
GH16 UNK
GH16
GH16
GH16
A new sub-family
of GH16 ?
~25% identity with
AgaA and CgkA
Phylogenetic tree of the GH16 family from Zobellia
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Zg3628 AgaF
Zg3640 AgaG
Zg1017 AgaH
Zg3376 AgaI
Zg2600 AgaE
Zg4267 AgaC
Zg4203 AgaA
Zg3573 AgaB
Zg4243 AgaD
Zg236 CgkA
Zg2431 LamA
Zg367 LamB
Zg1010 LamC
Zg1014 LamD
Zg1021
Z3347
LicA Bl
74
100
96
63
59
95
67
48
46
33
74
37
38
41
0.5
GH16
GH16 UNK
GH16 UNK
GH16
GH16
GH16
Phylogenetic tree of the GH16 family from Zobellia
Overexpression of
Zg1017 & Zg2600
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Zg1017
Zg2600
Activity screening on cell wall extracts
from seaweeds
• Inactive on agarose and carrageenans
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Zg1017
Zg2600
Activity screening on cell wall extracts
from seaweeds
L6S = L-galactose-6-sulfate
LA = 3,6-anhydro-L-galactose
Zg2600 (PorA) et Zg1017 (PorB) are the first beta-porphyranases
• Main end product: Porphyran disaccharide (L6S-G)
• Hydrolysis of beta-1,4 glycosidic linkage
a-1,3 b-1,4 • Inactive on agarose and carrageenans
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PorB PorA
Crystal structure of the b-porphyranases PorA and PorB
Molecular surface (Basic residues are colored in dark blue)
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W131
L6S L6S
G
G
-4
-3
-2 -1
E144
E139S
W56
H53
R133
R59
Catalytic residues
Structure of PorA in complex with porphyran
tetrasaccharide
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R59
H53
R133
W67
R70
I141
R252
L6S MES
PorA PorB
L6S -2
LA -2
G -1 AgaB
No pocket in b-agarases:
Steric clash with L6S at subsite -2
agarases cannot degrade
porphyran
Conserved basic residues at subsite -2 are critical for
porphyran recognition
Hehemann et al (2010) Nature
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Discovery of a new GH family in Zobellia
• 5 paralogous proteins distantly related to GH43 (~15% sequence identity)
• Always localized in gene clusters with CAZymes and sulfatases
• Zg3597 and Zg4663: soluble expressed in the MARINE-EXPRESS project
(Groisillier et al, 2010, Molecular Cell Factories)
• Purification and crystallization of Zg3597 and Zg4663
• Reducing sugar activity screening on our collection of algal polysaccharides
and cell wall extracts
Hypothesis: new GH specific for algal sulfated polysaccharides?
Unsuccessful…
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?
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?
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?
Surrounded by b-agarases, b-porphyranases and b-galactosidases
Zg4663: a a-1,3-galactosidase specific for agarocolloids?
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Neoagarobiose (DP2)
a-1,3
Test of the activity of Zg4663 on oligo-agars
released by the b-agarase AgaB
Thin layer chromatography
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Neoagarobiose (DP2)
a-1,3
1121 (DP7)
3,6-anhydro-L-galactose
815 (DP5)
509 (DP3)
m/z (M+Na+)
Test of the activity of Zg4663 on oligo-agars
released by the b-agarase AgaB
Lane 2 and 4: addition of Zg4663
Size of the products determined by mass
spectrometry (MALDI-TOF)
Thin layer chromatography
• Zg4663 is a 1,3-a 3,6-anhydro-L-galactosidase (last step of agar degradation)
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Neoagarobiose (DP2)
a-1,3
• Adopts a five-bladed b-propeller fold and forms a dimer by domain swapping
• Displays a zinc-dependent catalytic machinery
1121 (DP7)
3,6-anhydro-L-galactose
815 (DP5)
509 (DP3)
m/z (M+Na+)
• Zg4663 is a 1,3-a 3,6-anhydro-L-galactosidase (last step of agar degradation)
Rebuffet et al (2011) Environmental Microbiology
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Alginate lyases
CAZy family PL5 PL6 PL7 PL14 PL15 PL17 PL18
Z. galactanivorans enzymes
- AlyA4 AlyA6
AlyA1 AlyA2 AlyA5
AlyA7 Zg4327 AlyA3 -
Different specificities?
Different modes of action?
Different biological functions?
Zobellia genome contains
7 putative alginate lyases
L-Guluronic acids D-Mannuronic acids
O
OO O
O
O
OHOH
OH
HOOC
HOOC
O
HOOC
OH
OHO
OH
HOOC
OH
OH
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alyA3 SDR SusC-like SusD-like alyA2
GntR-like
permease
SDR
kdgK1
kdgF
alyA4 alyA5 alyA6
Alginate-related gene clusters?
alyA1
Transport
Outside
Periplasm
Cytoplasm
Sus-like system
permease
Regulation
GntR-like transcription regulator
Degradation
Alginate
4-deoxy-5-keto uronic acid
2-keto-3-deoxy-gluconate
ketodeoxyphosphogluconate
Alginate lyases
Gluconate dehydrogenases
(SDR)
kdgK
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pair 1 pair 2 pair 3 G A G A G A
G : glucose A : alginate
Primer pairs
alyA3 SDR SusC-like SusD-like alyA2
GntR-like
permease
SDR
kdgK1
kdgF
alyA4 alyA5 alyA6
Primer pair 1 Primer pair 2
Primer pair 3
Gene expression with alginate vs glucose
Identification of two alginolytic operons!
Thomas et al (2012) Env Microbio
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Characterization of alginate-responsive genes
Medium-throughput cloning strategy
Recombinant, soluble proteins
Confirmation of the enzymatic activity for 6 new proteins from Z. galactanivorans • Alginate lyases: AlyA1, AlyA4, AlyA5 and AlyA7 • 2-dehydro-3-deoxygluconokinase: Zg2614 • 2-dehydro-3-deoxy-D-gluconate 6-dehydrogenase: Zg2622
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Characterization of alginate-responsive genes
Medium-throughput cloning strategy
Recombinant, soluble proteins
AlyA1 and AlyA5 Two new lyases from the PL7 family
-1 +1 +2 +3
-1 +1 +2 +3
AlyA1: endo-lyase
AlyA5: exo-lyase
Thomas et al, (2013) JBC
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Conclusions & perspectives
Conclusions
• Algae-associated bacteria are essential sources of novel marine enzymes
• A (semi)-rational strategy for the discovery of novel enzymes: combination of phylogenomics, comparative genomics and biochemical approaches
• Importance of libraries of natural polysaccharides / oligosaccharides for the screening of real functions
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Conclusions & perspectives
Conclusions
• Algae-associated bacteria are essential sources of novel marine enzymes
• A (semi)-rational strategy for the discovery of novel enzymes: combination of phylogenomics, comparative genomics and biochemical approaches
• Importance of libraries of natural polysaccharides / oligosaccharides for the screening of real functions
Perspectives: start of two new projects in 2015
ALGOLIFE: processing macroalgae for high value-added human and animal
health/nutrition products
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Thank you!
Marine Glycobiology Group
Aurore Labourel Jan-Hendrik Hehemann
François Thomas
Etienne Rebuffet