grm 2011: keynote address-1: integrated breeding: impacts and challenges on crop productivity in...
TRANSCRIPT
Integrated Breeding: Impacts and Challenges on crop productivity in
Indian Poor Farmers’ Fields
Rapeseed and mustard growing area in India
Breeding objectives in B. juncea
Increase in yield
Increase in yield potential
Hybrids / pure line breeding
Stabilization breeding
Disease and pest resistance
Quality
Oil
Meal
17 10 13 26 30 4
Blight White rust Powdery mildew
Low erucic, low glucosinolate varieties Aphid Others
Cluster B
Similarity Coefficient (F value)
0.82 0.86 0.91 0.95 1.00
Varuna
Pusa Jai Kisan
RH 30 Pusa Bold
Kranti
TM 4
RLM 198 Chinensis-nigra
Sej-II
PNMB Hanzahang Gaoyou Cai
Shi Yian Kuyou Cai
D3A 2
AS 1
D1S 1.3
D3S 33
PS 6 TS 2
ATC 94395
Acc. No. 404 Donskaja IV
Zem1
Skorospieka II
Cutlass
Domo IV
Malopoloska
Acc. No. 2548
Secus
Acc. No. 409 Heera
Cluster A
Cluster C
Two major gene pools in B. juncea
Indian
East European
Srivastava et al (2000) TAG
Breeding of oilseed mustard
Germplasm, In vitro protocols, breeding
Two major Pollination control Molecular mapping Germplasm for
gene pools methods disease resistance
1st generation hybrids DMH-1, DMH-11
2nd generation hybrids
3rd generation hybrids
4th generation hybrids
WGS Quality Disease QTLs
resistance
Performance of hybrid DMH-1 in Coordinated trials in
zone II during Rabi 2004-05, 2005-06 and 2007-08
S.
No.
Strain /
Hybrid
Seed Yield Oil Yield 1000
seed
weight
(g)
Oil
Content
(%) kg/ha %
increase
over
kg/ha %
increase
over
1 DMH-1 2053 - 812 - 3.6 38.38
2 Varuna (NC) 1592 29 616 32 5.4 37.29
3 Kranti (NC) 1701 21 677 20 4.1 39.19
4 RL-1359 (ZC) 1811 13 719 13 4.3 38.95
NC - National Check; ZC - Zonal Check
Reference: AICRPRM reports (2005, 2006, 2008)
Released by ICAR in 2009 for Zone II
(Hissar, Sriganganagar, Navgaon, Delhi, Bawal, Ludhiana, Bathinda)
Performance of proposed hybrid DMH-1 in Coordinated trials in zone III during Rabi 2004-05, 2007-08 and 2008-09
S. No
Strain/ hybrid
Seed Yield Oil Yield 1000 seed
weight (g)
Oil content
(%)
Days to maturity
Kg/ha % increase
over
Kg/ha % increase
over
1 DMH1(PH) 2027 - 825 - 3.5 40.48 125
2 Varuna(NC) 1534 32.1 612 34.8 5.4 39.94 129
3 Kranti (NC) 1681 20.6 689 19.7 3.8 40.79 128
4 RL-1359 / Maya (ZC)
1697 19.4 675 22.2 4.6 39.65 131
Released by ICAR in 2010 for Zone III (Morena, Pantnagar,Faizabad, Kanpur, Kota)
PH- Proposed Hybrid; NC - National Check; ZC - Zonal Check Reference: AICRPRM reports (2005, 2006, 2008)
Performance of DMH-1 in farmers field during 2009-10
Location No. of
Farmers
surveyed
Average
yield of
DMH-1 (q/ac)
Average
yield of
Check
(q/ac)
%
increase
over
check
Alwar & Dousa Distt 25 8.60 6.39 34.59
Sriganganagar 13 9.4 8.11 15.91
Rewari 11 8.60 7.85 9.55
Bharatpur & Agra 14 8.10 7.95 1.89
Year Wise Production & Area Coverage Under DMH-1
Year 2007-08 2008-09 2009-10 2010-11
Qty Sold (in Q)
104.92 466.49 770.86 1428
Area coverage (in ha)
4,197 23,324 38,543 71,400
Location: Agra
Check (Rohini) Hybrid (DMH-1)
Construct : AMVLbar-spacer-TA29(870)bn
RB LB 35SdePr bar barnase 35SpA TA29(279) ocspA
Construct : debar-TA29(279)bn
Transformation frequency : 0-2%
No. of explants used : 10,090
Frequency of male sterile plants : 2% (1/50)
Construct : AMVLbar-TA29(870)bn
Transformation frequency : 0-3.5%
No. of explants used : 7855
Frequency of male sterile plants : 59% (27/46)
Transformation frequency : 2-6%
No. of explants used : 5997
Frequency of male sterile plants : 73% (71/97)
RB LB 35SPr bar barnase 35SpA TA29(870) ocspA AMV
RB
LB 35SPr bar barnase 35SpA TA29(870) ocspA spacer AMV
bar::barnase constructs
Jagannath et al. (2001) Mol Breeding
RB
BamHI BamHI
LB 35SdePr bar bswt 35SpA TA29(870) ocspA
RB LB
BamHI BamHI
35SdePr bar bswt 35SpA TA29(870) ocspA bswt A9(1500) 35SpA
a) bar/TA29-bswt
c) bar/A9bswt::TA29bswt
b) bar/A9(280)TA29(330)-bswt
d) bar/A9bsmod::TA29bswt
RB bswt 35SpA TA29(330) A9(280)
BamHI
LB 35SdePr bar ocspA
BamHI
bar::barstar constructs
RB 35SpA LB
BamHI BamHI
35SdePr bar bswt TA29(870) ocspA bsmod A9(1500) 35SpA
Bisht et al. (2004) Mol Breeding
SEED YIELD (Kg/Ha) OF DMH-11 UNDER BIO-SAFETY TRIAL-1 (BRL-1) DURING RABI 2010-11
S No Entry
ICAR Centre
Total Mean % Increase
over Kumher Alwar Sgnagar
1 Varuna (barnase) 1986 1789 2513 6287 2096 24%
2 EH-2 (Barstar) 1730 1842 2455 6026 2009 29%
3 Varuna 1866 1741 2670 6278 2093 24%
4 EH-2 1793 1716 2182 5691 1897 37%
5 DMH-11 2285 2515 3000 7801 2600
6 Maya/RL-1359 (ZC) 2057 1767 2287 6112 2037 28%
BRL-I Trial, Kumher
BRL-I Trial, Navgaon
Crossability study at Bawana
BRL-I Trial, Sri Ganganagar
Molecular map of B. juncea genome
By 2003
(TAG, 106:607-614)
By 2011
1. Total no. of markers 1029 2160
2. Types of markers
a) AFLP 996 1282
b) RFLP 33 70
c) SSR 0 279
d) Gene markers
e) Intron spanning markers
0
0
13
778
3. Total length (cM) 1628.7 1850.2
4. Assigning LGs to A and
B genomes
- 10 A genome LGs
8 B genome LGs
5. Traits tagged
a) Erucic acid - Candidate genes
b) Linoleic acid - Candidate genes
c) Seed coat colour d) Glucosinolates
- -
SSR markers
Candidate genes
wg2a4a 0.0
e39m55h163 7.1
p65t76v198 20.2
p79t78v127 29.2
p65m62h265 33.3
e54t75h129 40.9
e47t64v352 45.9
e39m50h446 54.8
p62t78h185 63.1
p53t76h368 68.4
FAE1.2 72.6 e79t78v205 76.1
e50t67v375 87.1
p32t77v268 110.1
p65m62h181 118.4
e31m50v273 122.9 ea-2
(39
%)
B7
e31m60v98 0.0 e48t78v400 3.6
e34m50v82 12.6
wg2h1 19.4
p62m39v124 27.8
e39m50v500 36.0
e54t71h380 46.7 e32t66h142 49.2
e32t66v191 FAE1.1 50.1
e31m60v195 52.6
p65t90v258 59.9
ea-1
(60
.8%
)
A8
Tagging of two loci of erucic
acid (ea1 and ea2) by
candidate gene polymorphism
Development of Low GSL Varuna through DH
Varuna X Heera No. of low GSL plants Year
F1 DH Expected - 50% Actual - 44%
7/ 752 1999 - 2000
X Varuna
BC1 DH
11 / 1263 2000 - 01
Expected - 75% Actual - 61%
BC2 DH
Expected - 87.5% Actual - 75%
8 / 728 2001 - 02
X Varuna
X Varuna
BC3 DH Expected - 93.7% Actual - 82%
13 / 1380 2002 - 03
X Varuna
BC4 DH
22/3479
2003 - 04 27/ 3550
Expected - 96.8% Actual - 86%
BC5DH 2004 - 05
x Varuna
Expected - 96.8% Actual - 86%*
*No further increase in Varuna genome due to linkage drag
Mapping of GSL-elong, GSL-alk, GSL-pro and Myb 28 genes
in B. juncea
p58m71h154 0.0
ec3f1 7.7
p65m47v155*v 15.1
e46t65h723 26.5
p62t78v278*v 36.1
p48t94v336*v 47.7
ec2c12 58.9
p80m48v270 66.9
p33t83h171 76.7 p58m35h184 82.5
GSLelonR1/R2 84.2 e34m43v260 86.8 A75 88.3
e34m43h335 91.3
A2
GSLalkR2 0.0
e44m50h155 6.3
e34m43h360 13.1
e31m60h440 18.6
e58m44v202 27.2
p33t83v197 37.1
DGAT1 43.1
e50t66v251 49.1
e48m39v773 60.4
Dfr-R 65.0
p58m71v952 78.9
e34m62v390*h 87.7
e34m43v310 95.7
p53m47h350 102.1
e46m59v304 110.4
e48t78h132 120.5
p48t94h261 129.2
A9
e35t69h736 0.0
e31m50h174 10.8
e34m62v219 25.3
p64t66h390 34.5
FAD6.2 43.5
e51t78v450 53.9
e47t64h233 62.4
e34m43h350 71.5 e31m62v199 75.3 N17 79.8 GSLalkN1.2 80.9 N19 83.3
e31m60h223 90.7
N183 102.7
p53t76v392 110.5
e62t65h147 121.6
N89 130.6
B4
WR 0.0
p62m59h197 9.5
e62m32h183 16.4
e47m43h330 23.2
e31m58v101 33.4 e48m39v100 35.3 GSLelonR3 37.0 ec4c11 40.0
p58t68v455 47.1
A131 54.1 p63t78v400 57.2
A4
e50m32v292 0.0
p33t66v200 16.7
e32t66h147 24.5
wg3g9a 32.8
e31m50h335 40.3
e35t63h280 45.6
p64m32h258 63.9
p53m42v139 74.2
p62m59v465 82.1
p33m38v260 97.4
e44m39v89 104.0
e34m49v163 113.5 p48m67h295 115.5 GSLelonN2 117.5 N15 120.
6 e62m32v179 124.5
e44m50h105 137.2
p63t78h267 148.2
B8
e32t70v718 0.0
p61m57v318 8.1
e45m34h420*v 15.4 e31m61v99 24.0 R42.1 27.2 R187 27.9 GSLelonR4/R5
myb28a 28.4
31.6 ec2d8 34.3
wg6d6 44.4
e47t74v415 53.1 p33m32h332 58.7 e31m47h153 63.6
GSLalkR1.1 64.2 e62m32h170 67.9 e48m39v160 71.4
p53m47h173*h 81.3
A1 90.2
e39t74v736 99.1
p33m38h274*h 104.9
e34m62h290 117.0
A3
102.1
e42t70h733
e34t74h719 0.0
e39m55h425
p32m66v901
e58m40h85
91.7 p33m32h148
p53m42h188 77.3
58.0 e44m39h131
e45m34h101
8.4
16.7
24.1
41.8
B1
wg1g6b 0.0
p48t67v370 9.1
p63m50h215 18.1
p62t78v265 29.5
p53t91h252 36.1
e34m50v205 46.4
e62m33h241 55.6
N135 63.0
p87m38h176 72.6
e31m62h288 77.7
GsLelonN1 89.3
B6
Gsl-pro
myb28b
Ann.locus
Pyramiding the desired recombinants
A3 A2 B1 A9 Activity Schedule Identification
of desired
recombinants
2006-07
311-1 (BC5DH)
gsl3
570-497 (BC4DH) 402-23 (BC5DH) 411-166 (BC4DH)
gsl5
gsl1
gsl2 QTL-NIL
identification
and crosses
2006-07
F1
X
gsl3
Size of BC4DH and BC5DH population genotyped: 1595; No. of makers: 18
gsl1 gsl5
X
gsl2 Three loci
pyramiding 2007, Leh
gsl1/+
gsl2/+
gsl3/+
Desired
2007-08 Four loci
pyramiding
Desired gsl1/+
gsl3/+
gsl5/+
X
gsl2
Desired heterozygotes were identified
through MAS after screening 100
individuals in each cross
MAS – DH and four loci
low GSL lines DH and three loci
low GSL lines 2008-09
gsl1/gsl1, gsl2/gsl2, gsl3/gsl3 gsl1/gsl1, gsl2/gsl2, gsl3/gsl3, gsl5/gsl5
At5g05920 2.4
At5g13190a 12.8
At5g53920 31.9
At1g74640a 48.5
At5g27740b 77.6
ELONG R1 90.1
At5g61970 95.4
Msl S
qm
s
At4g35530 6.9
At4g21105 18.3
At1g74640b 27.8 ELONG R4/Myb28a 29.9
At3g54670b 37.1
At3g10572b 50.6
At4g01897a 62.5 ALK R1 64.2
At2g30200a 77.5 Tsw
S
ql
Pbr
ALK R2 0.6 At5g27560 8.6
At5g63905 21.5
At2g14170a 34.2
At2g19260 44.9
At3g46560 57.9
At2g03060 68.3
At1g22860 78.5
At3g55430a 90.6
At3g62620 104.4
At2g20490b 118.0
At2g22370 129.7
At3g06483 8.8
At3g10572a 19.1
At3g20920 30.1
At5g47890 44.6 At5g27830
46.2
At5g66290 53.2
At1g58220a 55.3
At3g23980 61.6
At3g14850 74.3
At3g12260b 84.9
At3g13062 100.4
A2 A3 A9 B1
117
75
25
118
12
123
4-5
63520
ELONG R1
61970
?
27740b
74640b
ELONGR4
Myb28a
14045
05755 05590 54670b
?
Myb28b
Tagging of loci involved in glucosinolate
biosynthesis in B. juncea
?
Negative yield QTL from low GSL parent
Development of ’00’ Hybrid in mustard
Activity Timeline
Summer 2011
Barnase F1 x ’00’ Varuna 2011 -12
Identification of barnase ’00’
Varuna 2012 - 13
Experimental ’00’ hybrid 2013-14
Field trial of ’00’ hybrid 2014 - 15
Barnase Varuna x ‘00’ Varuna
Fatty acid composition of ‘0’ erucic and ‘0’ erucic high oleic mustard lines
Oil types 14:0 16:0 18:0 18:1 18:2 18:3 22:1
Mustard Oil 0.0 2.63 1.15 12.09 16.17 12.09 46.87
Olive Oil 0.0 9.53 3.2 77.91 6.33 0.57 0.0
’00’ Canola 0.0 4.97 0.74 60.29 21.39 10.49 0.38
‘0’ Erucic mustard* 0.0 4.1 1.1 53.4 23.9 15.5 0.0
‘0’ Erucic high oleic mustard*
0.0 2.2 1.2 74.8 9.5 9.3 0.0
* These lines have been developed in this project
16:0 Palmitic acid, 18:0 Stearic acid, 18:1 Oleic acid, 18:2 Linoleic acid, 18:3 Linolenic acid, 22:1 erucic acid
Sivaraman et al. (2004) Mol Breeding
White rust
• Phenotyping of VH and TD mapping
populations identified two different loci
for white rust resistance
• The two loci are being transferred to
different B. juncea lines using tightly
linked markers
A4
At3g63420b*(3g150) 0.0 At3g54650(3g66) 2.4 At3g58500a*(3g165) At3g55440b(3C-18) 4.7 p62m59h197 5.5 At5g37480(5gAJ30k) At1g77550(174)
At4g13940*b(4C3) WR3 At5g37580(288)
6.4
At5g37630(5g59) 7.3 At5g37850(5gAJ31) 8.2 WR17 9.2 WR22 9.9 At5g41560(5gAJ35) 10.6 At1g66410(254d) At2g21150(2g135) WR26 11.8
At5g36880*(5g57v) At2g22000a(2g136) 12.5 At2g22640(2g137) 13.3 At2g24765a*(2C-9) 15.8 At2g25737(2C-10) p53m47h285 17.4 At2g28880b*(2C-15) 22.8 At1g69980(1gLP78) 25.2 e47m43h330 At2g30130*(AJ-2g11) 26.2 At2g31490a(2g59a) 29.8 At2g33040(417) At2g07440b*(2g105) At2g32520(2g61) 32.5
e31m58v101 36.4 GE-R3 At2g35790(2g66) 39.8 At2g36530c*(2C-22) 42.1 At2g37040*(ec4c11) 43.1 At2g37150*(wg4a4) 43.7 At2g40690*(wg5b2) At2g40765b(2g74b) 53.3 At1g76540(183b) 55.3 At2g42040(2g76) 56.6 p63t78v400 62.2 At2g44520(2g167) 74.9
Wh
ite
rust
Varuna-Heera
WR locus:7-17cM
TM4-Donskaja
WR locus:18-24cM
A5
Wh
ite
rust
At2g46390(2g82) 0.0
2g472.2 15.1 2g428 20.9 e47t70v258 e51t78t290 2g337 p32t77d380 27.3
2g415(b) e55t73t240 29.1 2g415(a) e50t66t380 29.6 e31m42v144 e35t63v351 e48t78v225 msa21 e34m62v89 e31m58v430 e31m61v136 e34m62v157 e31m49v336 e34m62t160 2g55(NCB)
32.6
e31m49v295 34.4 e31m50t98 43.9
p32t77v301 p32t77d310 56.9 3g14 57.5
2g385 64.5 e34m62d143 66.5 5g218 68.6 e35t63v240 70.4
Marker-assisted introgression of two white rust
resistance loci into popular Indian varieties of mustard
Donskaja IV AcB1-A5.1
X EH-2 AcB1-A4.1
F1
DH (296 lines)
Foreground selection
AcB1-A5.1 – (At2g36360)
AcB1-A4.1 - (At5g41560 and At5g41940)
Line no. 67, 98, 144 and 207
• contain two resistance loci
• Early maturity
• Good plant type
Pusa bold
Varuna
Pusa Jai Kisan
Rohini
EH-2
X
F1 BC1
Foreground and background
selection
BC3 Selfing / DH
Erucic acid
No. of
Genes
Indian Gene
Pool
EE Gene
Pool
Glucosinolates
Seed coat color
Oil content
Seed size
Pod density
Height
+
B
+
+
+
+
-
-
Linolenic content
Linoleic content
-
-
+
Y
-
-
2r
5r
2r
7 loci
4 loci
?
2 loci
3 loci
3 loci
B3
e47m43h258 0.0
At3g54670a 14.6
At3g55005a* 15.6
At3g54130 17.3
e55t63h137*h 22.2
At4g04190 29.8
e34m43v140*h 32.6
At3g47610* 36.1
At3g45620* 37.4
At3g43610 38.8
At4g03150 40.5
e46m59v402 42.0
At4g00585a 44.5
At4g01310 47.3
p53m48v227 48.3
At4g01897b 50.9
At4g10930 55.9
At2g26590a 58.8
At4g10050 59.6
e31m61v235 61.2
At2g34860 62.9
At1g14620a At2g44050
68.1
p33m67v370 70.7
At2g41530 73.2
At2g45690b 78.3
e50t70v238 82.5
At2g07340 88.5
At3g47370* 90.0
e44m50h298 93.1
At4g09820* 93.7
At4g07666b 94.2
At5g59140a 99.8
e31m42h150 103.6
At5g17410* 108.3
At5g17840 108.8
At5g15750 110.1
p87t77v400 113.3
At5g13480* 113.8
At5g13030 115.0
p65t71h400 125.3
At5g09310a 126.9
At5g01220 p53t82h195
131.8
N
M
O
P
J
W
R
o
I
a
G
P P
rim
ary
bra
nches (
15.2
%)
Secondary
bra
nches (
9.3
%)
Sili
qua p
er
main
shoot
(17.1
%)
Sili
qua p
er
pla
nt
(11.4
%)
Sili
qua le
ngth
(8.9
%)
Test w
eig
ht
(13.1
6%
) Sili
qua d
ensity (
6.9
5%
)
Sili
qua d
ensity (
7.3
2%
)
A3
At5g06240b* 0.0
e34m62h290 9.2
R71 26.2
At3g46180* 30.3
A1 At2g36930a 35.9
At2g31490b 41.1
At2g36305 42.8
At2g36530b* 43.5
At2g40765c 44.5
At2g42780 47.7
p40m82v935 48.9
At2g45690a 50.2
At2g25740a 53.1
At2g26590b 53.7
At2g23390 54.5
At5g50320 55.4
At2g23090b 58.3
e62m32h170 59.6
GA-R1 At4g02980 63.5
At4g01897a 65.2
p33m32h332 At3g04460
69.5
At3g04950a 70.3
wg7a11 77.6
At3g10572b 79.2
At3g15120 84.1
At3g16640*b At3g17300a
84.9
At3g21400 87.5
p45m43v250 90.2
At3g54670b 93.0
At2g05990* At2g05755
94.7
At2g12462* 95.7
At2g12400* 97.3
GE-R5 GSLelonR4 GE-R4
100.2
At1g74640b 102.0
At3g52300 At3g51260c*
104.5
At4g17900a* At4g16060b
106.9
At4g21400 108.2
At4g23100a At4g20150b
109.2
At4g21105 110.4
At4g24440 113.4
At4g17050a 114.1
At4g28830 118.4
At2g19110* 120.3
At4g30310 At4g30790b
120.9
At4g35530 At4g36960b*
121.8
At4g26240b 123.5
At4g00585b 127.2
e32t70v718 129.2
R
J
I
O
F
N
T
U
G-H
s
j
a
w
Sili
qua le
ngth
(5.0
6%
) T
est w
eig
ht
(11.6
1%
)
At2g27490 At3g50230* 55.3
e39m32h153 58.9
At1g23440* 60.4
At2g15430b* 66.5
At2g04235 At3g24315
67.5
At2g06510* 68.2
At2g16860 69.1
At2g19560 At2g18410
70.4
E
N
I
B
G
H f
ec5a6 0.0
p53m42h153 11.5
At1g68310*a 17.6
At1g67170*a 19.8
p62t78v211 22.7
At1g79040 29.7
At1g79950* 31.5
At3g56290 37.6
At3g55005c* 38.4
At3g53190a* 41.5
At2g28880a* 49.5
52.7
A7 Pla
nt h
eig
ht (2
8.2
2%
)
Prim
ary
bra
nches (2
9.6
8)
Secondary
bra
nches (7
.74%
)
Secondary
bra
nches (1
1.1
4%
)
Siliq
ua p
er p
lant (7
.98%
)
Siliq
ua le
ngth
(7.9
2%
) S
eeds p
er s
iliqua (1
3.5
9%
)
Test w
eig
ht (9
.68%
) S
iliqua d
ensity
(21.6
0%
)
Days to
flow
er (1
2.8
4%
)
Oil c
onte
nt (8
.93%
)
A10
e34m43h119 0.0 At1g02870 0.7
At1g04950* 5.9
e50m32h84 10.8
At5g59140b 14.2
At5g20250* 19.9 p53m70v126 21.5
At5g15400a 35.2 At5g14920* 36.9 At5g13190b 40.0
At5g11960 44.3 p58m35v180 46.2
At5g09310c 52.6
p48m67v150 55.9
At5g06240a* 59.5 At5g05920 62.0
ec5a7 65.9
At5g01160 71.3 p62m66v931 72.5
A
W
R
Pla
nt h
eig
ht (2
4.2
2%
)
Prim
ary
bra
nches (2
3.2
6%
)
Secondary
bra
nches (1
8.8
5%
)
Siliq
ua p
er m
ain
shoot (3
5.2
6%
)
Siliq
ua p
er m
ain
shoot (7
.69%
)
Siliq
ua p
er p
lant (1
2.2
1%
)
Siliq
ua le
ngth
(24.3
1%
)
Seeds p
er s
iliqua (2
4.9
8%
)
Test w
eig
ht (2
0.0
5%
)
Siliq
ua d
ensity
(6.7
5%
)
Days to
flow
er (4
3.6
4%
)
Oil c
onte
nt (2
0.3
0%
)
Four major QTL
for seed size
Meta-analysis for fine mapping of QTL
cluster on A10
At1g02870 0.0e50t66h372 1.9e62t65v520 4.1
e45m34v225 4.9At5g59140 9.2
e39m50v415 11.6e60t65v275 13.3p79t78d67 16.4
At5g16280 27.3
p63t78d239 35.2
e34m62t170 44.4
At5g09310 49.5
PL
HT
-5
PB
R-3
SB
R-6
MS
L-2
MS
L-3
SQ
MS
-2
SQ
P-3
SQ
L-5
SQ
L-6
SS
Q-4
TS
W-6
SQ
DY
-3
DF
-3
OIL
-4
A10-TD
e34m43h119 0.0At1g02870 0.7
e50t66h372 2.4e62t65v520 5.9e50m32h84 10.0At5g59140 13.1
e60t65v275 13.9e58t75v160 15.1At5g20250 17.8At5g18670 20.3At5g17270 25.7At5g16330 28.1At1g72550 30.5
At5g15400a 32.6At5g14920 34.5
At5g13190b 37.8wg8g7 41.2
Ni4-A03 44.1
At5g09310 49.9
p48m67v150 52.9
At5g06240a 56.4At5g05920a 58.9
ec5a7 62.8At5g07690b 65.6
At5g01160 68.7p62m66v931 70.0
PL
HT
-3
PB
R-2
SB
R-5
SB
R-6
SQ
MS
-2
SQ
MS
-3S
QM
S-4
SQ
P-5
SQ
L-6 S
SQ
-4
TS
W-8
SQ
DY
-4
DF
-5
OIL
-4
A10-VH
Meta-analysis
A10-INT
e34m43h119 0.0p62m45h450 2.4p58m59h121 4.9
Na12-E09(A137) 6.2
e31m42t380 10.8Ra2-E03(R65) 13.4
p53m70v126 18.2
At5g18680(386) 21.8
At5g17270(217-2a) 25.9At5g16360(352) 28.1At1g72550(213) 30.8
At5g15840(5g-131) 33.0At5g14920(5C15) 35.4At5g13190b(5g19) 38.8At5g19510(254b) 42.2
Ni4-A03(N1190) 45.3
p33m71v921 50.2
p48m67v150 54.2
At5g05920a(5g8) 58.9
At5g07690b(NCB) 66.8
p62m66v931 71.1
A10-INT
A10-TD
A10-VH
At1g02870 0.0e50t66h372 1.9e62t65v520 4.1
e45m34v225 4.9At5g59140 9.2
e39m50v415 11.6e60t65v275 13.3p79t78d67 16.4
At5g16280 27.3
p63t78d239 35.2
e34m62t170 44.4
At5g09310 49.5
PL
HT
-5
PB
R-3
SB
R-6
MS
L-2
MS
L-3
SQ
MS
-2
SQ
P-3
SQ
L-5
SQ
L-6
SS
Q-4
TS
W-6
SQ
DY
-3
DF
-3
OIL
-4
A10-TD
e34m43h119 0.0At1g02870 0.7
e50t66h372 2.4e62t65v520 5.9e50m32h84 10.0At5g59140 13.1
e60t65v275 13.9e58t75v160 15.1At5g20250 17.8At5g18670 20.3At5g17270 25.7At5g16330 28.1At1g72550 30.5
At5g15400a 32.6At5g14920 34.5
At5g13190b 37.8wg8g7 41.2
Ni4-A03 44.1
At5g09310 49.9
p48m67v150 52.9
At5g06240a 56.4At5g05920a 58.9
ec5a7 62.8At5g07690b 65.6
At5g01160 68.7p62m66v931 70.0
PL
HT
-3
PB
R-2
SB
R-5
SB
R-6
SQ
MS
-2
SQ
MS
-3S
QM
S-4
SQ
P-5
SQ
L-6 S
SQ
-4
TS
W-8
SQ
DY
-4
DF
-5
OIL
-4
A10-VH
Meta-analysis
A10-INT
e34m43h119 0.0p62m45h450 2.4p58m59h121 4.9
Na12-E09(A137) 6.2
e31m42t380 10.8Ra2-E03(R65) 13.4
p53m70v126 18.2
At5g18680(386) 21.8
At5g17270(217-2a) 25.9At5g16360(352) 28.1At1g72550(213) 30.8
At5g15840(5g-131) 33.0At5g14920(5C15) 35.4At5g13190b(5g19) 38.8At5g19510(254b) 42.2
Ni4-A03(N1190) 45.3
p33m71v921 50.2
p48m67v150 54.2
At5g05920a(5g8) 58.9
At5g07690b(NCB) 66.8
p62m66v931 71.1
A10-INT
Meta-analysis
A10-INT
e34m43h119 0.0p62m45h450 2.4p58m59h121 4.9
Na12-E09(A137) 6.2
e31m42t380 10.8Ra2-E03(R65) 13.4
p53m70v126 18.2
At5g18680(386) 21.8
At5g17270(217-2a) 25.9At5g16360(352) 28.1At1g72550(213) 30.8
At5g15840(5g-131) 33.0At5g14920(5C15) 35.4At5g13190b(5g19) 38.8At5g19510(254b) 42.2
Ni4-A03(N1190) 45.3
p33m71v921 50.2
p48m67v150 54.2
At5g05920a(5g8) 58.9
At5g07690b(NCB) 66.8
p62m66v931 71.1
A10-INT
A10-TD
A10-VH
Different Mapping Populations Developed in B. juncea
Cross Type of
population
Total markers
available
Traits of interest
Varuna x Heera
(VH)
1200 DH lines,
465 RILs
AFLP- 1290
RFLP- 70
IP- 749
Microsatellites- 189
Gene markers- 32
Seed size (5.2 TSW : 2.6 TSW),
Oil content (42% : 36%),
Oil and meal quality
TM4 x Donskaja
(TD)
134 DH lines AFLP- 585
IP- 318
Microsatellites- 8
White rust resistance,
Oil content (41% : 45%)
Donskaja x EH2
(DEH)
296 DH lines IP- 323
Microsatellites- 113
Gene markers- 8
Oil content (45% : 36%),
Oil and meal quality
EH2 x J8
(EHJ8)
1000 DH lines Oil content (36% : 49%),
Oil and meal quality
EH2 x Pusa Jai Kisan
(EHPJ)
300 DH lines Seed size (2.6TSW : 7.0 TSW),
Oil content (36% : 42%),
Oil and meal quality
NDDB / MDFVPL
Farming Communities Funding and Production of Seeds
DBT Funding
ICAR
Technology Development and trials UDSC
Trials
SFCI & Private Companies Seed production
Technology mapping
Basic work/ Genomics/Pre-breeding/ Breeding/Field application
Open source – Germplasm/Pre-breeding
CG Institutions – need to spearhead global agricultural research
Challenges Ahead
Collaborations
Dr. Arundhati Mukhopadhyay Prof. Akshay Pradhan
Dr. Vibha Gupta Dr. Pradeep Burma
Dr. Y. S. Sodhi Dr. Naveen Bisht
Dr. N. Arumugam Dr. Indira Sivaraman
Dr. Arun Jagannath Dr. N. Ramchiary
Mr. Jagdish Verma Dr. Priya Panjabi
Mr. B. S. Yadav Dr. Amrendra
Dr. Sarita Sharma Dr. Lakshmi K. Padmaja
Dr. Satish Yadav Mr. Diwakar Nandan