good afternoon, agents. please take out a writing utensil and place your backpack below your desk
TRANSCRIPT
Good afternoon, Agents.
Please take out a writing utensil and place your backpack below your
desk.
Crime Scene Investigation: Macromolecule Identification
Period 4 Debriefing Meeting
Crime scene #UNI091811
At 3:15 PM on Friday, September 16th 2011, an iPod belonging to Jane Doe was stolen.
Remnants of food found at the scene have been taken as evidence. Four suspects have been questioned, and food items from their lockers confiscated.
LIBRARY 9/18/2011
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CASE #UNI091811
CASE #UNI091811
CASE #UNI091811
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CASE #UNI091811
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CASE #UNI091811
Sketch by crime artist
Your task is to identify the food item in the evidence based on your biochemistry knowledge of macromolecules.
So what do you know about macromolecules?
CARBOHYDRATES
PROTEIN NUCLEIC ACID
LIPID
monosaccharide
amino acid
fatty acid
nucleotide
polysaccharide
Each team will be given 3 types of samples: 1.Dry evidence2.Liquid evidence3.One food sample from the
suspects:
SUSPECT FOOD SAMPLE CONFISCATED
A Strawberry jam cookies
B Buttered bread
C Pretzels
D Bean burrito
TEAM NAME FOOD SAMPLE
Team Red Strawberry jam
Team Orange
Cookies
Team Yellow
Butter
Team Green
Bread
Team Blue Pretzels
Team Purple
Beans
You have been assembled into color-coded teams.
The results of each team’s suspect food data will be compiled at 1:15 PM with our second debriefing meeting.
From this meeting, you will be able to identify your unknown evidence and single out the culprit.
We will take the time now to assign a number 1-6 to each
agent in your team.
Agent #1: Polysaccharide expertAgent #2: Monosaccharide expertAgent #3: Protein expertAgent #4: Lipid expertAgent #5: RecorderAgent #6: Recorder
Agent #1: Polysaccharide expert
You will use Iodide to determine the presence of starch.
BASIC PROCEDURE1.Put on lab apron, gloves, and
goggles.2.Label test tubes3.Pour out dry evidence into weigh
boat. Use back end of spoon to scoop and pour evidence.
4.Add water to the line, tap to mix well.
5.Add biochemical indicator, tap to mix well.
6.Relay observation to recorder.
Agent #1: Polysaccharide expert
Add 5 drops of Iodide to determine the presence of starch.
Iodide solution changes from
clear orange to deep blue-black in presence of
starch
Agent #2: Monosaccharide expert
Add 8 drops of Benedict’s solution to determine the presence of glucose.
Benedict’s solution changes from clear
blue to thick yellow-orange-red in presence of
glucose
Agent #3: Protein expert
Add 8 drops of Biuret solution to determine the presence of protein.
Biuret’s solution changes from clear
light blue to lavender-brown in
presence of protein
Agent #4: Lipid Expert
Smear the liquid sample onto a paper bag to determine the presence of lipids.Only you will be responsible for the LIQUID evidence.
You will have until 1:27 PM to complete this investigation.
Good luck teams.
CASE #UNI091811
• Return unused dry evidence back into tube
• Place dirty test tubes into water tub—don’t worry about gunk!
• Throw away lipid brown papers
• Return racks, water bottles into shoe box.
Actual Samples and EvidencePolysaccharide test Monosaccharide test
Protein test
Thank you scientists!
TEAM NAME
CULPRIT EVIDENCE
Team Red C Pretzels
Team Orange
D Bean burrito
Team Yellow
AStrawberry jam
cookies
Team Green
C Pretzels
Team Blue
D Bean burrito
Team Purple
B Buttered bread