gn adams cv 6-10-15
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Gregory N. Adams3430 Shaw Ave, Apt 2Cincinnati, OH, 45208
EDUCATION
Graduate School: July 2007 – Aug 2012: PhD, Case Western Reserve University; Department of Pathology,
Molecular and Cellular Basis of Disease Training Program; Alvin H. Schmaier Laboratory in the Division of Hematology/Oncology; Thesis defense accepted by committee 8/29/12; Degree conferred Jan 2013
o Thesis title: “Prolylcarboxypeptidase protects from vascular dysfunction and promotes vascular repair”
Undergraduate: Sept 2002 – May 2006: John Carroll University, University Heights, Ohio; Major in
Biology: Cumulative GPA – 3.38; Major GPA – 3.78
PROFESSIONAL EXPERIENCE
Sept 2012 – Current: Post-Doctoral Research Fellow; Cincinnati Children’s Hospital Medical Center; Division of Experimental Hematology; Joseph Palumbo/Jay Degen Laboratory
Sept 2014 – Dec 2014: Visiting Lecturer; Miami University-Ohio, Hamilton Campus; Microbiology Department
May 2006 – July 2007: Research Assistant; Case Western Reserve University; Department of Dermatology; Nicole Ward Laboratory
June 2005 – Dec 2005: Undergraduate Research Assistant; Lerner ResearchInstitute; Cleveland Clinic Foundation; Paul DiCorleto Laboratory
PUBLISHED BIBLIOGRAPHY
Adams GN, Stavrou EX, Fang C, Merkulova A, Alaiti MA, Nakajima K, Morooka T, Merkulov S, Larusch G, Simon DI, Jain MK, Schmaier AH. “Prolylcarboxypeptidase promotes angiogenesis and vascular repair.” Blood. 2013, 122:1522-1531
- Figure 2G (upper right panel) selected for the journal cover image of the 8/22/13 edition of Blood
Fang C, Stavrou E, Schmaier AA, Grobe N, Morris M, Chen A, Nieman MT, Adams GN, Larusch G, Zhou Y, Bilodeau ML, Mahdi F, Warnock M, Schmaier AH. “Angiotensin-(1-7) and Mas decrease thrombosis in Bdkrb2-/- mice by increasing NO and prostacyclin to reduce platelet spreading and GPVI activation.” Blood. 2013, 121:3023-32
Adams GN, Schmaier AH. “The Williams-Beuren Syndrome – A Window into Genetic Variants Leading to the Development of Cardiovascular Disease” PLoS Genet. 2012 Feb; 8(2): e1002479.
Bandyopadhyay S, Harris DP, Adams GN, Lause GE, McHugh A, Tillmaand EG, Money A, Willard B, Fox PL, DiCorleto PE. ”HOXA9 methylation by PRMT5 is essential for endothelial cell expression of leukocyte adhesion molecules.” Mol Cell Biol. 2012 Apr;32(7):1202-13
Loyd CM, Diaconu D, Fu W, Adams GN, BS, Knutsen DA, Wolfram JA, McCormick TS and Ward NL. “Transgenic overexpression of keratinocyte-specific VEGF and Ang1 in combination promotes wound healing under nondiabetic but not diabetic conditions” Int J Clin Exp Pathol. 2012; 5(1):1-11.
Adams GN, Larusch GA, Stavrou E, Zhou Y, Nieman MT, Jacobs GH, Cui Y, Jain MK, Shariat-Madar Z, Okada Y, D’Alecy LG, Schmaier AH. “Murine Prolylcarboxypeptidase Depletion Induces Vascular Dysfunction With Hypertension and Faster Arterial Thrombosis” Blood. 2011 Apr 7;117(14):3929-37.
LaRusch GA, Mahdi F, Shariat-Madar Z, Adams G, Sitrin RG, Zhang WM, McCrae KR, Schmaier AH. “Factor XII Stimulates ERK1/2 and Akt Through uPAR, Integrins, and the EGFR To Initiate Angiogenesis” Blood. 2010 Jun 17;115(24):5111-20
INVITED ORAL PRESENTATIONS
July 1, 2013: 24th Congress: International Society on Thrombosis and Hemostasis, Amsterdam, NL – “Prolylcarboxypeptidase Promotes Angiogenesis and Vascular Repair.”
Dec 13, 2011: Special Symposium on the Basic Science of Thrombosis and Hemostasis, American Society of Hematology Annual Meeting, San Diego, CA – “Prolylcarboxypeptidase Promotes Endothelial Cell Proliferation and Vascular Repair”
Dec 6, 2010: American Society of Hematology Annual Meeting, Orlando, FL -- “Prolylcarboxypeptidase Deficiency Is a Risk Factor for Arterial Thrombosis and Hypertension”
July 13, 2009: 22nd Congress: International Society on Thrombosis and Hemostasis, Boston, MA – “Prolylcarboxypeptidase Murine Hypomorphs Are Hypertensive and Prothrombotic”
FIRST AUTHOR POSTER PRESENTATIONS
Dec 7, 2014: American Society of Hematology Annual Meeting, San Francisco, CA – “Thrombin Drives Colorectal Pathogenesis at the Level of Primary Tumor Growth, Tumor Invasion and Metastasis”
July 3, 2013: 24th Congress: International Society on Thrombosis and Hemostasis, Amsterdam, NL – “Thrombin and Fibrinogen Support Prostate Tumor Growth in Mice.” (Moderator for “e-Poster” session on cancer and thrombosis)
Dec 2011: American Society of Hematology Annual Meeting, San Diego, CA – “Prolylcarboxypeptidase Promotes Endothelial Cell Proliferation and Vascular Repair”
Sept 2011: Case Cardiovascular Center (CCC) Research Retreat, Cleveland, OH – “Prolylcarboxypeptidase Promotes Endothelial Cell Proliferation and Vascular Repair”
June 2010: CWRU Department of Medicine Retreat, Cleveland, OH – “Prolylcarboxypeptidase Murine Hypomorphs Are Hypertensive and Prothrombotic From Increased Reactive Oxygen Species”
Dec 2008: American Society of Hematology Annual Meeting, San Francisco, CA – “Mice Deficient in Prolylcarboxypeptidase Have a Prothrombotic and Hypertensive Phenotype”
July 2008: Hemostasis Gordon Conference, Waterville Valley, NH – “Mice Deficient in Prolylcarboxypeptidase Have a Prothrombotic and Hypertensive Phenotype”
June 2007: Society For Investigative Dermatology Annual Meeting, Los Angeles, CA – “Angiopoietin-1 Can Enhance Wound Healing In a Re-Epithelialization-Independent Manner”
March 2006: John Carroll University “A Celebration of Scholarship” – “Binding Partners for HOXA9 in Endothelial Cells”
INSTITUTIONAL PRESENTATIONS
March 5, 2013: CCHMC Monthly Post-doctoral Meeting, “Thrombin Promotes Prostate Cancer Progression.”
March 28, 2012: CWRU Cardiovascular Research Institute Seminar – “Prolylcarboxypeptidase (PRCP) protects from vascular dysfunction and promotes vascular repair”
May 4, 2011: CWRU Department of Pathology Seminar – “Murine Prolylcarboxypeptidase Depletion Induces Vascular Dysfunction With Hypertension and Faster Arterial Thrombosis”
Sept 24, 2010: CWRU Cancer Center Research Seminar – “Prolylcarboxypeptidase Murine Hypomorphs are Hypertensive and Prothrombotic From Increased Reactive Oxygen Species”
Oct 14, 2009: CWRU Department of Pathology Seminar – “Prolylcarboxypeptidase Murine Hypomorphs Are Prothrombotic and Hypertensive”
Sept 21, 2009: CWRU Hematology/Oncology Research Seminar - “Prolylcarboxypeptidase Murine Hypomorphs Are Prothrombotic and Hypertensive”
June 17, 2009: CWRU Cardiovascular Research Institute Seminar -“Prolylcarboxypeptidase Murine Hypomorphs Are Prothrombotic and Hypertensive”
Sept 22, 2008: CWRU Hematology/Oncology Research Seminar – “Prolylcarboxypeptidase Murine Hypomorphs Are Prothrombotic and Hypertensive”
EDUCATIONAL EXPERIENCE Sept 2014 – Dec 2014: Part-time Visiting Lecturer; Miami University-Ohio, Hamilton campus;
Microbiology 131 – “Perspectives in Community Health”: Developed a previously existing 2 credit hour course into a 3 credit hour course
Aug 2014: University of Cincinnati undergraduate Capstone Poster Symposium judge
June 2014 – Aug 2014: Mentored a University of Michigan student in the UC summer undergraduate research program, resulting in a Capstone Poster Symposium poster titled “Thrombin Drives the Growth and Migration of Prostate and Colon Carcinoma”
Aug 2013: University of Cincinnati undergraduate Capstone Poster Symposium judge
AWARDS May 2015: Young Investigator Award, International Society on Thrombosis and Haemostasis May 2013 - Current: Pelotonia Fellowship Program—Post-Doctoral; The James Cancer
Center, Ohio State University (75% of salary at CCHMC) Dec 2011: Travel Award, American Society of Hematology Annual Meeting Jan 2011 - Jan 2012: NIH T32 Training Grant #5T32HL7147-35 Dec 2010: Travel Award, American Society of Hematology Annual Meeting Dec 2008: Travel Award, American Society of Hematology Annual Meeting 2003-2006: JCU Track and Field school record holder in 4 events and 16-time All-Conference May 2004: NCAA All-American, Track and Field (800 meter run) Fall 2002 & Spring 2006: John Carroll University Dean’s List Dec 2002: Member of Phi Eta Sigma National Honor Society
RESEARCH CONFERENCES ATTENDED
Dec 2014: American Society of Hematology, San Francisco, CA July 2013: International Society on Thrombosis and Hemostasis, Amsterdam, NL Dec 2012: American Society of Hematology, Atlanta, GA Dec 2011: American Society of Hematology, San Diego, CA Sept 2011: Cardiovascular Center Retreat, Cleveland, OH June 2011: Proteases In Hemostasis and Vascular Biology FASEB, Carefree, AZ Dec 2010: American Society of Hematology, Orlando, FL July 2009: International Society on Thrombosis and Hemostasis, Boston, MA Dec 2008: American Society of Hematology, San Francisco, CA July 2008: Hemostasis Gordon Conference, Waterville Valley, NH June 2007: Society for Investigative Dermatology, Los Angeles, CA
TECHNICAL EXPERIENCE Establishment of new protocols : Chemiluminescent measurement of reactive oxygen species
(ROS) in tissue homogenates and vessels, high pressure liquid chromatography measurement of oxidized dihydroethidium (DHE), endothelial nitric oxide synthase uncoupling ROS assay, mouse plasma thrombin generation assay, primary embryonic mouse keratinocyte isolation, scratch cell migration assay, mouse “Gurtner” silicone-splinted excision wound model
Animal Models: Mouse blood pressure telemetry, vascular perfusion with fluorescent imaging, matrigel sub-cutaneous angiogenesis, biopsy punch mouse skin wound healing (splinted and unsplinted models), sub-cutaneous tumors, i.v. injection of metastatic tumor cells
Pharmacology : Binding characteristics of the oral thrombin inhibitor FM19, in vivo (mouse) inhibition of activated plasma kallikrein (PKSI-527, Pro-Phe-Arg-chloromethylketone, soybean trypsin inhibitor) and prolylcarboxypeptidase (Z-Pro-Pro-aldehyde-dimethyl acetal), osmotic pump (mito-TEMPO and bradykinin-B2 receptor agonist labridimil), oral delivery in mouse drinking water (apocynin, Tempol, doxycycline) and chow diet (Tempol)
Histology : Frozen cryostat sectioning (was responsible for maintaining Dermatology Department cryostat equipment), immunoperoxidase and immunofluorescent staining of frozen and paraffin sections, X-gal staining, H&E staining
Tissue Culture: Cell culture: Vascular (human umbilical vein, human micro-vascular, bovine aortic, bovine smooth muscle), Skin (primary human foreskin keratinocytes, primary embryonic mouse keratinocytes), Cancer (mouse colon [MC38], mouse prostate [TRAMP])
isolation of mouse embryonic fibroblasts, transfection with siRNA and plasmids, proliferation and apoptosis assays
Protein chemistry : Enzyme kinetics (activated protein C, prekallikrein, prolylcarboxypeptidase), immuno-precipitation, western blotting, antibody isolation, ELISA
Nucleic acids : RNA isolation from various organs and cell culture, real-time PCR, cloning