glial cells in drosophila
DESCRIPTION
Glial cells in Drosophila. 18.03.2011 Bahar. Drosophila genome. Celniker et al. 2003. Cytogenetic nomenclature. X : 1 - 20 2L : 21 - 40 2R : 41 - 60 3L : 61 - 80 3R : 81 - 100 4 : 101 - 102. Recombination. Meiotic recombination in oogenesis - PowerPoint PPT PresentationTRANSCRIPT
Glial cells in Drosophila
18.03.2011Bahar
Drosophila genome
Celniker et al. 2003
Cytogenetic nomenclature
X : 1 - 202L : 21 - 402R : 41 - 603L : 61 - 803R : 81 - 1004 : 101 - 102
Recombination
Meiotic recombination in oogenesis Bigger chance of recombination if the elements
are further apart Shuffled balancer chromosomes to prevent
recombination http://kyotofly.kit.jp/stocks/documents/Balancers.html
ONLY IN FEMALES!
Genetic representation
Ak/Y ; UAS Gal80ts, D::E/ Sco; F gal4, UAS RFP/ TM6b, Tb
XAk/ FM7; ∆B; P{EP}C/ TM3, Act GFP, Serhttp://flybase.org/static_pages/docs/nomenclature/nomenclature3.html
Glia
Non neural cells in CNS and PNS with ectodermic or hematopoietic origin, with the homeostasis function
Glia: Neuron1:1 Drosophila10:1 Mammals
Drosophila development atlas by Volker Hartenstein http://www.sdbonline.org/fly/atlas/00atlas.htm
Glial cells
Ransaohoff, 2010
Embryonic development
CNS: Lateral and midline glia. Neurons arise from neuroblasts and gmc. Glia arise from glioblast and neuroglioblast
Lateral glia development initiated by gcm. (transcription factor)
repo downstream of gcm, another transcription factor
Knoblich 2001
Embryonic development
Commissures and bundles
Bashaw 2010
AxonsNeurons
Banerjee 2007
Larval glia
NMJs Brain Imaginal discs Zuo
2008
Aigouy 2004Soustelle 2007
Bristles
Neumüller 2009
Useful drivers Repo gal4 (aka M1B): pan glial driver. CNS
and PNS entire glia, starting from early embryonic development, continues after terminal differentiation. Larval and adult glia.
Gcm gal4: embryonic glial driver. neuroglioblast/glioblast-specific driver, limited to development. Restricted expression in larva.
Glial drivers Mz97 gal4: embryonic and larval glia (Read,
2009). Peripheral and wrapping glia. But it’s not restricted to glia.
Nrv2 gal4: embryonic to adult CNS glial (Read, 2009)
Eaat gal4: larval cortex glia in CNS, perisynaptic glia in PNS (Read, 2009)
Glial drivers
Moody gal4, surface glia in CNS (Lievens, 2008)
Rhomboid gal4, embryonic mesectodermal/midline glial driver
Gliotactin gal4, peripheral glia, for NMJs (Lavery, 2007)
Gal4-NP577, cortex glia (PNS) (Kato, 2008)
Glial drivers
MZ709, embryonic and first star larval, peripheral glia (Lavery, 2007)
158 gal4, lateral glial driver
New tools for gliahttp://onlinelibrary.wiley.com/doi/10.1002/glia.21133/full