gh sensitivity dna assay de72901273 2016-04-18 …...2016/04/18 · 4/18/2016 4:25:31 pm data path:...
TRANSCRIPT
Instrument Name: DE72901273 Firmware:Serial#:Assay Information:
C.01.069DE72901273
Instrument Information:
Assay Origin Path: C:\Program Files\Agilent\2100 bioanalyzer\2100expert\assays\dsDNA\High Sensitivity DNA.xsy
Assay Class:Version:Assay Comments:
High Sensitivity DNA Assay1.03Copyright © 2003-2010 Agilent Technologies
Chip Information:
Chip Comments:
Type: G2939A
Chip Lot #:Reagent Kit Lot #:
2 3 TS-1
TS-2 TS-3 TS-4
TS-5
2100 Expert (B.02.08.SI648) © Copyright 2003 - 2009 Agilent Technologies, Inc. Printed: 4/18/2016 5:07:42 PM
2100 expert_High Sensitivity DNA Assay_DE72901273_2016-04-18_16-25-31.xad Page of1 8
Created:Modified:
4/18/2016 4:25:31 PM4/18/2016 5:01:17 PMData Path:
High Sensitivity DNA AssayC:\...gh Sensitivity DNA Assay_DE72901273_2016-04-18_16-25-31.xad
Assay Class:
Electrophoresis File Run Summary
Sample Name Sample Comment Rest. Digest Status Observation Result Label ResultColor
23TS-1TS-2TS-3TS-4TS-5sample 8sample 9sample 10sample 11Ladder
Chip Lot # Reagent Kit Lot #
Chip Comments :
2100 Expert (B.02.08.SI648) © Copyright 2003 - 2009 Agilent Technologies, Inc. Printed: 4/18/2016 5:07:42 PM
2100 expert_High Sensitivity DNA Assay_DE72901273_2016-04-18_16-25-31.xad Page of2 8
Created:Modified:
4/18/2016 4:25:31 PM4/18/2016 5:01:17 PMData Path:
High Sensitivity DNA AssayC:\...gh Sensitivity DNA Assay_DE72901273_2016-04-18_16-25-31.xad
Assay Class:
Electrophoresis File Run Summary (Chip Summary)
Peak table for sample 3 : TS-1Peak Size [bp] Conc. [pg/µl] Molarity [pmol/l] Observations1 35 125.00 5,411.3 Lower Marker2 61 20.03 493.93 320 12.76 60.44 482 2,023.85 6,355.95 510 498.80 1,480.56 523 281.10 813.87 534 393.35 1,116.48 555 1,735.63 4,739.89 10,380 75.00 10.9 Upper MarkerRegion table for sample 3 : TS-1From[bp]
To [bp] Corr.Area
% ofTotal
Average Size[bp]
Size distribution inCV [%]
Conc.[pg/µl]
Molarity[pmol/l]
Color
200 1,000 4,407.6 98 524 20.3 5,029.50 15,386.9
TS-1
Overall Results for sample 3 : TS-1Number of peaks found: 7 Noise: 0.6
Corr. Area 1: 4,407.6
2100 Expert (B.02.08.SI648) © Copyright 2003 - 2009 Agilent Technologies, Inc. Printed: 4/18/2016 5:07:42 PM
2100 expert_High Sensitivity DNA Assay_DE72901273_2016-04-18_16-25-31.xad Page of3 8
Created:Modified:
4/18/2016 4:25:31 PM4/18/2016 5:01:17 PMData Path:
High Sensitivity DNA AssayC:\...gh Sensitivity DNA Assay_DE72901273_2016-04-18_16-25-31.xad
Assay Class:
Electropherogram Summary
Peak table for sample 4 : TS-2Peak Size [bp] Conc. [pg/µl] Molarity [pmol/l] Observations1 35 125.00 5,411.3 Lower Marker2 439 1,593.50 5,499.13 464 337.92 1,103.84 478 1,747.37 5,537.35 10,380 75.00 10.9 Upper Marker6 12,137 0.00 0.0Region table for sample 4 : TS-2From[bp]
To [bp] Corr.Area
% ofTotal
Average Size[bp]
Size distribution inCV [%]
Conc.[pg/µl]
Molarity[pmol/l]
Color
200 1,000 4,109.7 99 472 18.2 4,003.43 13,412.7
TS-2
Overall Results for sample 4 : TS-2Number of peaks found: 4 Noise: 0.4
Corr. Area 1: 4,109.7
2100 Expert (B.02.08.SI648) © Copyright 2003 - 2009 Agilent Technologies, Inc. Printed: 4/18/2016 5:07:42 PM
2100 expert_High Sensitivity DNA Assay_DE72901273_2016-04-18_16-25-31.xad Page of4 8
Created:Modified:
4/18/2016 4:25:31 PM4/18/2016 5:01:17 PMData Path:
High Sensitivity DNA AssayC:\...gh Sensitivity DNA Assay_DE72901273_2016-04-18_16-25-31.xad
Assay Class:
Electropherogram Summary Continued ...
Peak table for sample 5 : TS-3Peak Size [bp] Conc. [pg/µl] Molarity [pmol/l] Observations1 35 125.00 5,411.3 Lower Marker2 430 6,014.92 21,175.73 900 3.09 5.24 3,688 1.64 0.75 6,595 2.02 0.56 10,380 75.00 10.9 Upper MarkerRegion table for sample 5 : TS-3From[bp]
To [bp] Corr.Area
% ofTotal
Average Size[bp]
Size distribution inCV [%]
Conc.[pg/µl]
Molarity[pmol/l]
Color
200 1,000 6,171.1 99 449 18.2 6,020.54 21,161.4
TS-3
Overall Results for sample 5 : TS-3Number of peaks found: 4 Noise: 0.5
Corr. Area 1: 6,171.1
2100 Expert (B.02.08.SI648) © Copyright 2003 - 2009 Agilent Technologies, Inc. Printed: 4/18/2016 5:07:42 PM
2100 expert_High Sensitivity DNA Assay_DE72901273_2016-04-18_16-25-31.xad Page of5 8
Created:Modified:
4/18/2016 4:25:31 PM4/18/2016 5:01:17 PMData Path:
High Sensitivity DNA AssayC:\...gh Sensitivity DNA Assay_DE72901273_2016-04-18_16-25-31.xad
Assay Class:
Electropherogram Summary Continued ...
Peak table for sample 6 : TS-4Peak Size [bp] Conc. [pg/µl] Molarity [pmol/l] Observations1 35 125.00 5,411.3 Lower Marker2 422 1,382.13 4,958.03 451 431.83 1,452.24 462 360.77 1,182.45 488 1,192.65 3,702.06 756 15.98 32.07 10,380 75.00 10.9 Upper MarkerRegion table for sample 6 : TS-4From[bp]
To [bp] Corr.Area
% ofTotal
Average Size[bp]
Size distribution inCV [%]
Conc.[pg/µl]
Molarity[pmol/l]
Color
200 1,000 3,657.5 98 459 19.7 3,592.21 12,483.2
TS-4
Overall Results for sample 6 : TS-4Number of peaks found: 5 Noise: 0.4
Corr. Area 1: 3,657.5
2100 Expert (B.02.08.SI648) © Copyright 2003 - 2009 Agilent Technologies, Inc. Printed: 4/18/2016 5:07:42 PM
2100 expert_High Sensitivity DNA Assay_DE72901273_2016-04-18_16-25-31.xad Page of6 8
Created:Modified:
4/18/2016 4:25:31 PM4/18/2016 5:01:17 PMData Path:
High Sensitivity DNA AssayC:\...gh Sensitivity DNA Assay_DE72901273_2016-04-18_16-25-31.xad
Assay Class:
Electropherogram Summary Continued ...
Peak table for sample 7 : TS-5Peak Size [bp] Conc. [pg/µl] Molarity [pmol/l] Observations1 35 125.00 5,411.3 Lower Marker2 268 15.71 88.93 460 967.80 3,187.34 500 344.42 1,044.65 546 1,107.70 3,075.56 1,497 18.74 19.07 1,740 8.93 7.88 1,881 8.76 7.19 2,128 5.84 4.210 2,763 5.19 2.811 3,572 5.94 2.512 4,931 6.28 1.913 6,395 7.29 1.714 7,758 6.70 1.315 10,380 75.00 10.9 Upper Marker16 12,014 0.00 0.017 12,830 0.00 0.018 13,475 0.00 0.019 14,378 0.00 0.020 14,808 0.00 0.021 15,667 0.00 0.022 16,527 0.00 0.023 18,290 0.00 0.024 18,977 0.00 0.025 20,482 0.00 0.026 20,998 0.00 0.0
TS-5
Overall Results for sample 7 : TS-5Number of peaks found: 25 Noise: 0.3
Corr. Area 1: 3,955.7
2100 Expert (B.02.08.SI648) © Copyright 2003 - 2009 Agilent Technologies, Inc. Printed: 4/18/2016 5:07:42 PM
2100 expert_High Sensitivity DNA Assay_DE72901273_2016-04-18_16-25-31.xad Page of7 8
Created:Modified:
4/18/2016 4:25:31 PM4/18/2016 5:01:17 PMData Path:
High Sensitivity DNA AssayC:\...gh Sensitivity DNA Assay_DE72901273_2016-04-18_16-25-31.xad
Assay Class:
Electropherogram Summary Continued ...
... Peak table for sample 7 : TS-5Peak Size [bp] Conc. [pg/µl] Molarity [pmol/l] Observations27 21,858 0.00 0.0Region table for sample 7 : TS-5From[bp]
To [bp] Corr.Area
% ofTotal
Average Size[bp]
Size distribution inCV [%]
Conc.[pg/µl]
Molarity[pmol/l]
Color
200 1,000 3,955.7 91 536 26.2 2,424.55 7,475.2
2100 Expert (B.02.08.SI648) © Copyright 2003 - 2009 Agilent Technologies, Inc. Printed: 4/18/2016 5:07:42 PM
2100 expert_High Sensitivity DNA Assay_DE72901273_2016-04-18_16-25-31.xad Page of8 8
Created:Modified:
4/18/2016 4:25:31 PM4/18/2016 5:01:17 PMData Path:
High Sensitivity DNA AssayC:\...gh Sensitivity DNA Assay_DE72901273_2016-04-18_16-25-31.xad
Assay Class:
Electropherogram Summary Continued ...