GerstelGerstel頂空進樣頂空進樣 HeadspaceHeadspace

MPSMPS三合一系統(( 液體，頂空，液體，頂空， SPME)SPME)

Page 2

Supports mutliple injection ports

Syringe-only concept

Modular

Saves bench space

Highly adaptable

Fully controlled through the advanced MASter software

3 Sample trays: - 98 x 2 ml Vials - 200 x 1 ml Vials - 32 x 10/20 ml Vials

Universal syringe holder for syringes from 10 µl up to 1000 µl

Liquid injections from 1 µl up to 1000 µl in conjunction with a CIS

Large Volume Calculator

Injection speed programmable

Lower detections limits

Multiple Headspace Extraction in conjunction with GERSTEL-ColdInjectionSystems

Syringe Headspace Injections

Syringeholder heatable up to 150° C

Hot Needle Injection

3 sample trays : 98 x 2 ml Vials 32 x 10/20 ml Vials

Agitator for 6 samples

Automated Solid-Phase-Micro-Extraction (SPME)

Liquid and Headspace SPME possible

3 sample trays : 98 x 2 ml Vials 32 x 10/20 ml Vials

Thermal sample equilibration and agitation

For all common SPME fibers

Bake out station for SPME fibers

Dilute

Extractions

Derivatisation

Adding internal standards

應用應用

Page 13

Page 14

其他應用其他應用 (( 以下為一些資料的應用以下為一些資料的應用 ))• Traditional HSS Applications

Forensic: Blood Alcohol Analysis

Pharmaceutical: Residual Solvent Analysis (USP 467, EP)

Environmental: VOC in Drinking Water

Consumer Products: Flavor and Fragrance

• New Application Extension

High Temp. Polymer

Semi-volatile active compounds: Pesticides Analysis

Page 15

Blood Alcohol - Dual Column Blood Alcohol - Dual Column ConfirmationConfirmation

GC Oven: 35 oC isothermal

Inlet: Split/Splitless at 150 oC

*a two-hole ferrule (p/n 5062-3580) was used to connect both columns to the same inlet

Column I: DB-ALC1 (p/n 123-9134)30 m x 0.32 mm x 1.8 µm

Column II: DB-ALC2 (p/n 123-9234)30 m x 0.32 mm x 1.2 µm

Headspace oven: 60 oC

Vial equilibration time: 15 min.

Sample: 5ul of 0.08 g/dL standard mix in 10 ml vial

1. Acetaldehyde

2. Methanol

3. Ethanol

4. Acetone

5. 2-Propanol

6. Acetonitrile

7. Ethyl Acetate

8. Methyl-Ethyl Ketone

1.00 2.00 3.00 4.00 5.00

Column I

1.00 2.00 3.00 4.00 5.00

Column II

1

2

3 4

5

67

8

12

3 4, 6

5

7

8

G1888 Sales TrainingJan. 2004

Agilent Restricted Page 16

Residual Solvent in Pharmaceutical Residual Solvent in Pharmaceutical ProductProduct

min0 2 4 6 8 10 12 14

115

7

17

19

201

2221

18

16

2

3

4

5

6

13,14

12

8

9

11

10

Headspace Conditions: Oven Temp: 85 oC Loop Temp: 95 oC Transfer Line Temp: 110 oC Vial equilibration time: 10 min

GC Condition: Column: DB-624, 30 m x 0.53 mm x 3.0 um Carrier: Helium, 35 cm/sec, Constant Flow Oven: 40 oC (5 min) to 90 oC at 2 oC/min 90 oC to 250 oC at 30 oC/min Injection: Headspace, 180 oC, Split 7/1 Detector: FID, 260 oC

1 Methanol 2 Ethanol 3 Ether4 Acetone 5 i - Propanol6 Acetonitrile7 Methylene Chloride8 t - Butanol9 Hexane10 Propanol11 Methylethylketone

12 Ethylacetate13 Tetrahydrofuran14 s - Butanol15 Chloroform16 Cyclohexane17 Benzene18 Heptane19 Trichloroethylene20 1,4 - Dioxane21 Pyridine22 Toluene

Page 17

VOC in Water – HS/GC/MSD VOC in Water – HS/GC/MSD ApplicationApplication

2.00 4.00 6.00 8.00 10.00 12.00 14.00 16.00

1

2

3

4

5

6

7

8

910

11

1213

14

15

16

17

18

1920

21

22

23

24

25

26

2728

29 30 31

Sample: 40 ppb, 5ml water in 10 ml vial, 2 grams NaCl added

Column: 20 m x 0.18 mm x 1.0 DB-VRX (PN: 121-1524)

Headspace oven temp: 75 oC

Equilibration time: 20 min

MSD Scan range: 45 – 260 amu

1. Chloromethane, 2.Vinyl chloride, 3. Bromomethane, 4. Chloroethane, 5.Trichloromonofluoromethane, 6. 1,1 Dichloroethene, 7. Methylene chloride, 8. 1,2 dichloroethane, 9. Trichloromethane, 10. 1,2 dichloroethane, 11. 1,1,1 Trichloroethane, 12. Carbon tetrachloride, 13. Benzene, 14. 1,2 dichloropropane, 15. Trichloroethylene,

16. Bromodichloromethane, 17. 2 chloroethoxyethane, 18. cis 1,3 dichloropropene, 19. trans 1,3 dichloropropene, 20. 1,1,2 trichloroethane, 21. Toluene, 22. Dibromochloromethane, 23. Tetrachloroethylene, 24. Chlorobenzene, 25. Ethylbenzene, 26. Bromoform, 27. 1,1,2,2 tetrachloroethane, 28. 1,3 dichlorobenzene, 29. 1,4 dichlorobenzene, 30. 1,2 dichlorobenzene

Page 18

High Temp: Soda Bottle – PET Analysis High Temp: Soda Bottle – PET Analysis (new)(new)

Column: 30 m x 0.25 mm x 0.5 HP-5MS (PN 19091S-133)

Headspace equilibration time: 30 min

MSD Scan range: 35 – 250 amu

Sample: 0.5cm x 0.5 cm squares of soda bottle, 2.0 g in 20

ml vial under nitrogen

4.00 6.00 8.00 10.00 12.00 14.00 16.00 18.00 20.00 22.00 24.00 26.00 28.00 30.00

1 2 3

4

5

6

7

5.00 10.00 15.00 20.00 25.00 30.00 35.00 40.00 45.00 50.00 55.00

21

3

4

5

67

HSS Temp 100 oC

HSS Temp. 230 oC

1. Propylene glycol n-propylether,

2. Diketone alcohol ,

3. Mycene,

4. Octanol,

5. D-Limonene,

6. Terpinene,

7. Nonanal,

8. Decanal

1. Methyldioxolane,

2. Ethylene glycol,

3. Hydroxy ethylacetate,

4. Methylester acetic acid,

5. D-limonene,

6. Decanal,

7. n-Hexadecanoic acid

G1888 Sales TrainingJan. 2004

Agilent Restricted Page 19

Pesticide Analysis (HS/GC/MSD/VI inlet) Pesticide Analysis (HS/GC/MSD/VI inlet) (new)(new)Sample: 100 ng Pesticide mix in AcetoneFull evaporation method, ~ 1 ng on column injectedColumn: 30 m x 0.25 mm x 0.25um HP-5MSGC: 6890N with volatiles interface, Split 5 to 1MSD: 5973 inert

HSS: G1888A Conditions10 ml vialTemp: 225 oC, 15 min.

10.00 15.00 20.00 25.00 30.00 35.00

1

2

3

4

5 6 7 8

9 10 11 12

13 1415

16 17

18 19

20 21

22 23

24 2526 27

1. Dichlorvos

3. Vernolate

8. Lindane

15. Chlorpyrifos

17. P,p’-DDE

19. Propargite

21. Mirex

27. Fluvalinate

Page 20

Perfume Base for Consumer ProductsPerfume Base for Consumer Products

5.00 10.00 15.00 20.00 25.00 30.00 35.00

1

2

3 4

5

Headspace conditions: Temp: 100 oCEquilibrium Time 30 minEvaporation method, 3 drops in 10 ml vial

MSD: 5973 inert, scan 30 – 500 amuGC: 6890N with volatiles interfaceColumn: 30 m x 0.25 mm x 0.5um HP-5MS

1. Pinene,

2. 2,6 dimethyl 7-Octen-2-ol,

3. Phenylmethylester acetic acid

4. Vertenex,

5. Hexylsalicylate

Page 21

Brewed CoffeeBrewed Coffee

1. Acetaldehyde,

2. Acetone,

3. 2-methyl propanal,

4. 4. 2-butanone,

5. 2-methyl furan

6. Acetic acid,

7. 3-methyl butanal,

8. 2-methyl butanal

1.00 1.50 2.00 2.50 3.00 3.50 4.00 4.50 5.00 5.50

1

2

34

5

6

7 8

Headspace Conditions:

Temp: 80 oC

Time: 30 min

Sample: 10 ml coffee in 20 ml vial

MSD: 5973 inert, scan 30 – 300 amu

GC: 6890N with volatiles interface

Page 22

Orange OilOrange Oil

5.00 10.00 15.00 20.00 25.00 30.00

12

3

4

5

6

7

89

Column: 30 m x 0.25 mm x 0.50 HP-5MS

Headspace oven temp: 100 oC

Equilibration time: 20 min

MSD Scan range: 35 – 260 amu

Sample: 3 drops orange oil in 10ml vial

1. Dimethylvinylmethanol

2. nonane,

3. Pinene,

4. Phellandrene,

5. Myrcene,

6. Limonene,

7. 1-Octanol,

8. Linalool

9. Decanal

Page 23

Rose OilRose Oil

50.00

3

5.00 10.00 15.00 20.00 25.00 30.00 35.00 40.00 45.00

1

2

4

5

67

MS

D O

FF

Elu

tion

of p

hen

ylethyl

alcoh

ol

Headspace Conditions:

Temp: 225 oC

Time: 30 min.

Evaporation method, 3 drops in 10 ml vial

MSD: 5973 inert, scan 30 –300 amu

GC: 6890N with volatiles interface

1. Dimethylvinylmethanol

2. nonane,

3. Pinene,

4. Phellandrene,

5. Myrcene,

6. Limonene,

7. 1-Octanol,

8. Linalool

9. Decanal