GeniePlex Quantitative Multiplex Immunoassays

Quantitatively Measure 1-24 Analytes Per Sample!

ELISA Genie is а proprietary range of ELISA kits & multiplex assays developed bу Reagent Genie, а global life science reagents company based in London and Dublin.

Founded bу Colm Ryan PhD and Seán Mac Fhearraigh PhD, our goal is to provide you with premium quality ELISA kits along with excellent technical and logistics support, so you саn maximise your success.

GeniePlex Multiplex Immunoassays

ELISA Genie: Maximum Support

Standard Curves - Human 18-Plex

pg/mL

MFI

IL-2

IL-6

IL-8

MIG

IL-4

IL-10

IFNg

TNFa

IL-1b

IL-1RA

IL-12p70

IL-17A

MCP-1

MCP-3

RANTES

Eotaxin

IL-22

IP-10

100000

10000

1000

100

10

1100001000100101

GeniePlex: Quantitively Measure 1-24 Analytes Simultaneously!

GeniePlex is a bead-based multiplex immunoassay technology. It enables the simultaneous & quantitative detection of up to 24 analytes in as little as 15 μl sample on almost any flow cytometer. It’s like doing 24 different ELISA in every well!

Very Sensitive: Measure as low as <10 pg/ml of each analyteExcellent Dynamic Range: Lower limit < 20 pg/mL | Upper limit > 5,000 pg/mL High Precision & Accuracy: Intra-assay CV: < 10% | Inter-assay CV: < 20% | Recovery: 70-130%Low Sample Volumes: Use as little as 15 µl of sampleValidated: All assays fully tested for cross-reactivity in our lab

Phenomenal Performance

Comprehensive Choice of Targets: Up to 400 assays & custom formats available for human, mouse, rat, porcine, canine and primatesWide Range of Sample Types: Cell culture supernatants, saliva, plasma, cell/tissue lysates, serum, BALF, pleural and peritoneal fluids & moreMultiple Formats: 32-well and 96-well sizes available

Unbeatable Flexibility

Use Your Own Lab: Assays can be run on most validated flow cytometers (PE & either PE-Cy5 or APC detectors)Free Software: Analyse with commonly available software such as FCAP Software v3.0. Or, send us your data!Customisation: Can’t find your analyte in our extensive list? Then just ask our team of experienced scientists to help build your assay

Convenience Redefined

GeniePlex Multiplex Immunoassays

GeniePlex: Incredible Flexibility

GeniePlex Multiplex Immunoassays

0

50

100

150

200

250

Human Mouse Rat Primate Canine

GeniePlex Competitor B

GeniePlex: Choose from 400+ Analytes!

GeniePlex gives you the flexibility to create your own multiplex immunoassays. Whether you work with human, mouse, rat, non-human primate or even canine samples, simply choose your analytes from the species list and we will create your assay of choice. GeniePlex also

offers more choice than leading competitors.

No.

of A

naly

tes

GeniePlex Multiplex Immunoassays

GeniePlex: Multiplexing Simplified

1. Add GeniePlex antibody-bead conjugates to the wells

6. Read on a flow cytometer

7. Analyse 1-24 analytes

Wash 3X

2. Add Standards or Samples

3. Add Biotinylated Antibodies

4. Add Strepavidin-PE

5. Add Reading buffer Biotin

Wash 3X

Biotin

Wash 3X2

GeniePlex: Excellent Benchmarking Data

GeniePlex Multiplex Immunoassays

y = 1,0737x + 10,264R² = 0,9292

0500

100015002000250030003500

0 1000 2000 3000

Geni

ePle

x(p

g/m

L)

Luminex (pg/mL)

TNFαy = 0,8712x + 13,712R² = 0,9391

050

100150200250300350

0 200 400Ge

nieP

lex

(pg/

mL)

Luminex (pg/mL)

IL-17Ay = 0,9792x + 113,69R² = 0,9694

0

2000

4000

6000

8000

10000

0 5000 10000

Geni

ePle

x (p

g/m

L)

Luminex (pg/mL)

IL-6

y = 0,9414x - 6,6486R² = 0,9955

Geni

ePle

x(p

g/m

L)

0

1000

2000

3000

4000

5000

6000

0 5000 10000Luminex (pg/mL)

IL-2 IL-10y = 0,6238x - 3,149R² = 0,9699

0

200

400

600

800

0 1000 2000

Geni

ePle

x (p

g/m

L)

Luminex (pg/mL)

y = 0,653x - 0,1998R² = 0,9807

02468

10121416

0 10 20 30Luminex (pg/mL)

IL-4

y = 0,5948x + 8,0123R² = 0,9716

0100200300400500600700

0 500 1000 1500

Geni

ePle

x(p

g/m

L)

Luminex (pg/mL)

IFNγ

GeniePlex gives excellent performance versus Luminex technology. GeniePlex Human Th1/Th2/Th17 7-Plex assay and a Luminex multiplex assay kit were used to assay cell

culture supernatant samples from human PBMCs treated with various reagents.

Geni

ePle

x(p

g/m

L)

GeniePlex: Human Analytes

CCL11/SCYA11/EotaxinCCL2/SYCA2/MCP-1CCL5/SCYA5/RANTESCCL7/SYCA7/MCP-3/MARCCXCL10/IP-10IFN-gammaIL-1betaIL-1RAIL-2IL-10/CSIFIL-12p70IL-17A/CTLA-8IL-4IL-6IL-8/CXCL8IL-22/IL-TIFTNF-alphaG-CSF/CSF-3GM-CSF/CSF-2IFN-alpha 2IL-1alpha/IL-1F1IL-3IL-5IL-7IL-11IL-12/IL-23p40IL-13IL-17FIL-20/IL-10DIL-21IL-28A/IFNlambda 2IL-29/IFNlambda 1IL-33M-CSF/CSF1MPOTSLPVEGF-ACCL3/SCYA3/MIP-1alphaCCL4/MIP-1betaCX3CL1/FractalkineCXCL11/I-TACIL-9IL-15IL-16/LCFIL-17CIL-23IL-24IL-27IL-31IL-32IL-34LIFOSM/Oncostatin MSCF/MGF/KIT-LigandTIM-1/HAVCR/KIM-1TNF-beta/TNFSF1/LTA

VEGF-DANGPT-1/Angiopoietin-1BDNFbeta-NGF/NGFBEGFFGF basic/FGF-2/HBGH-2GASP-1/WFIKKNRPIFN-betaInsulinLeptin/LEPMIF/MMIF/GIFOPG/Osteoprotegerin/TNFRSF11B OPN/Osteopontin/BNSP PDGF-ABResistin/ADSF/XCP1Survivin/BIRC5TGF-alpha/TGF-type I/ETGFTHPO/Thrombopoietin/TPOVEGF-CBlyS/BAFF/sCD257/TNFSF13BCD14/sCD14E-Cadherin/CDH1/sCD324Endoglin/sCD105E-selectin/ELAM-1/sCD62EICAM-1/sCD54IL-2RA/sCD25PECAM-1/sCD31TNFRSF10c/sCD263/TRAIL-R3TNFRSF1A/sCD120a/sTNFRITNFRSF9/sCD137/4-1BBTNFSF11/sCD254/RANKLTNFSF14/sCD258/sLIGHTTNFSF6/sCD178/sCD95L/sFasLTNFSF8/sCD153/sCD30LTNFSF8/sCD154/sCD40LVCAM-1/sCD106VEGFR2/Flk-1/sCD309/KDRCCL13/SCYA13/MCP-4CCL15/SCYA15/MIP-1δ/MIP-5CCL19/SCYA19/MIP-3βCCL20/SCYA20/MIP-3αCCL21/SCYA21/6CkineCCL22/SCYA22/MDCCCL24/SCYA24/Eotaxin-2/MPIF-2CCL27/SCYA27/CTACKCCL28/SCYA28/MECCXCL13/BCA-1CXCL14/BRAKCXCL16/SCYB16CXCL5/ENA-78Gp130/sCD130IGFBP-1IGFBP-7IL-1R2/sCD121bIL-6Rα/IL-6R alpha/sCD126TNFRSF1B/sCD120b/sTNFR2/sTNFRIICCL1/SCYA1/I-309CCL14/SCYA14/HCC-1

CCL16/SCYA16/HCC-4CCL17/SCYA17/TARCCCL18/SCYA18/PARC/MIP-4CCL23/SCYA23/MPIF-1CCL25/SCYA25/TECKCCL26/SCYA26/Eotaxin-3CCL8/SCYA8/MCP-2CD279/sPD-1/sCD279CXCL1/MGSA/GROαCXCL12/SDF-1CXCL4/PF4CXCL6/GCP-2CXCL7/NAP-2BMP-2BMP-4Cystatin CFGF-1/FGF-acidic/HBGF-1FGF-19Flt-3 Ligand/Flt-3L/Flk-2LGDF-15/MIC-1/PTGFBGranzyme B/GZYMB/CTL1IGFBP-3IGFBP-5IGFBP-6Lipocalin-2/LCN2/NGALPAPP-ARAGE/AGER/sRAGESLP1/ALP/BLPIST2/IL-1R4/IL-1RL1TIMP-1TIMP-2ALCAM/sCD166CEACAM-1/sCD66aEpCAM/MK-1/TROP1/sCD326EPO-R/Erythropoietin ReceptorICAM-3/sCD50IL-1RI/sCD121aL-selectin/LECAM1/sCD62LNCAM-1/sCD56PDCD1LG2/PD-L2/sCD273PD-L1/sCD274/sB7-H1P-selectin/LECAM-3/sCD62PSLAMF4/2B4/NAIL/sCD244SLAMF8/BLAME/SBBI42/sCD353TNFRSF14/sCD270/sHVEMTNFRSF5/sCD40TNFRSF6/sCD95/sFasR VEGFR1/Flt-1Betacellulin/BTCCNTFFGF-4Follistatin/FSTIL-19KGF/FGF-7Lymphotactin/XCL1MIA/CD-RAPMidkine/MK1/NEGF-2NOV/CCN3/IGFBP-9NRG1-beta1/HRG1-beta1

PDGF-AAPDGF-BBPIGF/PIG-FTNFRSF13B/sCD267/TACITNFSF10/sCD253/TRAILAdiponectinAFP/Alpha-fetoprotein TNFSF13/sCD256/APRILArtemin/ARTN/NeublastinBIGH3/TGFBI/βIG-H3 CA 50CA-125/MUC16CA15-3CA19-9CD163/M130/sCD163 CD269/BCMA/TNFRSF17/sCD269CEA/CD66e/CEACAM5CRP/C-Reactive ProteinCXCL2/GROβ/MIP-2α/GRO2CXCL9/SCYB9/MIGEGFR/ErbB1EG-VEGF/PROK1/PK1EndostatinFerritinFGF-9/HBGF-9/GAFHGF/Hepatopoietin AGalectin-1/GalaptinGDNF/ATF-1Granzyme A/GZYMA/CTL3AIgAIgEIGFBP-2IL-18IL-25/IL-17EKeratin 19/KRT19/CK-19Klotho/KLA/KLLAP (TGF-beta1)/Latent TGF-beta1MMP-1/CLGN (Total)MMP-13/Collagenase 3 (Total)MMP-3/Stomelysin-1 (Total)MMP-7/PUMP-1 (Total)MMP-8/Collagenase 2 (Total)MMP-9/GELB (Total)TWEAK/TNFSF12/Apo3 LigandActivin AIgG1IgG2IgG3IgG4Procalcitonin/PCTAmphiregulinPro-BDNFReg3A/PAP2/PAPPAP-H/PBCGFElafin/PI3/Trappin2VAP-1/Vascular Adhesion Protein-1

GeniePlex Multiplex Immunoassays

Human

GeniePlex: Mouse, Rat, Primate, Canine & Custom Analytes

Eotaxin/CCL11G-CSF/CSF-3GM-CSF/CSF-2GRO alpha/KC/CINC1IFNgammaIL-10IL-12p70IL-13IL-17A/CTLA-8IL-1alpha/IL-1F1IL-1beta/IL-1F2IL-2IL-4IL-5IL-6IP-10/CXCL10MIP-1alpha/CCL3MIP-2/GRO beta/CINC3TNFalphabeta-NGF/NGFBCD254/RANKL/TNFSF11IL-12p40IL-15LeptinLIX/GCP2/CXCL5MCP-1/JE/CCL2MCP-3/MARC/CCL7RANTES/CCL5SDF-1/CXCL12sICAM-1/sCD54sVCAM-1/sCD106VEGFPDGF-ABTIMP-1FGF-2/FGF basic/HBGH-2PDGF-BBBDNF

RatCCL11/SCYA11/EotaxinCCL5/SCYA5/RANTESCCL7/SCYA7/MCP-3/MARCCXCL1/KC/CINC1/KC/Gro alphaCXCL9/MIGIFN-gammaIL-1alpha/IL-1F1IL-2IL-4IL-6IL-10/CSIFIL-12p70IL-17A/CTLA-8IP-10/CXCL10MCP-1/JE/CCL2TNF-alphaG-CSF/CSF-3GM-CSF/CSF-2IL-3IL-5IL-7IL-12/IL-23p40IL-13IL-15IL-17FIL-1beta/IL-1F2IL-21IL-22IL-28A/B/IFNlambda 2/3M-CSF/CSF-1 MIP-1 alpha/CCL3MIP-1beta/CCL4OSM/Oncostatin MTSLPBTC/BetacellulinCCL12/SCYA12/MCP-5CCL20/SCYA20/MIP-3αCCL27/SCYA27/CTACKCXCL16/SCYB16CXCL5/LIX/GCP2IL-1RAIL-9IL-23p19IL-25/IL-17EIL-2Rα/Ly43/sCD25IL-7R/IL-7Rα/sCD127Leptin/LEPLIFMIP-2/GRO beta/CINC3TNFSF11/CD254/RANKLTNFSF13B/BlyS/BAFF/sCD257VEGFActivin ABDNFBLC/CXCL13BRAK/CXCL14/MIP-2γ/BMAC

CCL21/SCYA21/Exodus-2 /6CkineFGF-1/FGF-acidic/HBGF-1FGF-2/FGF basic/HBGH-2FGF-9/HBGF-9/GAFHGF/HPTA/SFIL-11IL-20/IL-10DIL-33/NFHEV/DVS27MDC/CCL22/ABCD-1/STCP-1MIP-1γ/CCL9/CCL10/MRP-2/CCF18MIP-3β/CCL19/ELCOPG/Osteoprotegerin/TNFRSF11B TARC/CCL17TIMP-1CD10/Neprilysin/sNEP/CALLA/sCD10CD105/Endoglin/sCD105CD106/sVCAM-1CD119/sIFNγR1/IFNγRaCD120a/sTNFRI/TNFRSF1ACD120b/sTNFRII/TNFRSF1BCD121a/sIL-1R1CD14/sCD14CD153/sCD30L/TNFSF8 CD154/sCD40L/TRAP/TNFSF5CD266/sTWEAK-R/Fn14/TNFRSF12aCD27/sTNFRSF7/sCD27CD309/sVEGFR2/KDR/Flk-1CD54/sICAM-1CD62E/sE-selectin/ELAM1CD62L/sL-selectin/LECAM1CD62P/sP-selectin/ LECAM-3RAGE/sRAGE/AGERVEGFR1/Flt-1/sVEGFR1ANGPT-1/Angiopoietin-1beta-NGF/NGFBCD30/TNFRSF8/sCD30Eotaxin-2/CCL24/MPIF-2CRPCCL8/MCP-2CCL28/MEC/CCK-1EGF

MouseIL-12p70Eotaxin/CCL11IFNgammaIL-10/CSIFIL-17A/CTLA-8IL-1betaIL-1RAIL-2IL-4IL-6IL-8/CXCL8IP-10/CXCL10MCP-1/JE/CCL2MCP-3/MARC/CCL7RANTES/CCL5TNFalphabeta-NGF/NGFBFGF basic/FGF-2/HBGH-2IL-5MIG/CXCL9SDF-1TGFalpha/TGF-type I/ETGFVEGF-A

Primate

IFNγIL-2IL-6IL-8/CXCL8IL-10/CSIFIL-12p40IL-17AMCP-1/JE/CCL2SCF/MGF/KITLGTNFαVEGFInsulin

Canine

Custom PanelsCan’t find your species or analyte? Just let us know and we can work with you to develop new immunoassays and multiplex panels using the best-in-class validated antibodies

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