genetics newsgenetics news mystery sequence project lost book problem day friday unknown day monday
TRANSCRIPT
Genetics News
Mystery sequence project
Lost book
Problem Day Friday
Unknown Day Monday
Where to get help before the exam
Wednesday, Sept 20 6:30 PM - 8:00 PM E-107 Sarah Muller
Wednesday, Sept 20 7:00 PM - 9:00 PM UFA2107 Jennifer Lee
Thursday, Sept 21 4:00 PM - 5:00 PM S-215 Garrett Griffin
Friday, Sept 22 3:00 PM - 5:00 PM S-215 Jeff Elhai
Sunday, Sept 24 8:00 PM - 10:00 PM E-209 Karen Lewis
Monday, Sept 25 2:30 PM - 3:30 PM S-111 Sarah Muller
Monday, Sept 25 5:15 PM - 6:15 PM S-111 Sarah Muller
See Who We Are page on web.Click on Special Help Sessions.
Pathways: Making Autos
Pathways: Making Autos
Pathways: Making Autos
Pathways: Making Autos
Pathways: Making Autos
Pathways: Making Autos
Pathways: Making Autos
substrate
Pathways: Making Autos
substrateconsumed
Product
Pathways: Making Pigment
chemical A(vermilion color)
Enzymes
Cell
Pathways: Making Pigment
Modify chemical A
Enzyme X
Pathways: Making Pigment
Enzyme X
chemical B(cinnabar color)
Pathways: Making Pigment
Enzyme Y
Modify chemical B
Pathways: Making Pigment
Wild-type pigment(red color)
Enzyme Y
Pathways: Making Pigment
Accumulate wild-type pigment
X+ Y+
X+ Y-
X- Y+
Wild-type eye color
Cinnabar eye color
Vermilion eye color
When Pathways Go Wrong
X+ Y-
X- Y+
When Pathways Go Wrong
Imaginal disk tissue
Abdominal tissue
X+ Y-
When Pathways Go Wrong
Imaginal disk tissue
Abdominal tissue
Wild-type eye color
X- Y+
Pulse-Chase ExperimentDoes RNA move from nucleus to cytoplasm?
Pre pulse
UU
UU
U
UU
U
UU
UUU
U
RNA
Uracil
Pulse-Chase ExperimentDoes RNA move from nucleus to cytoplasm?
Pulse
UU
UU U
UU
U
RNA
Uracil
UU
U
UUUU
U
Pulse-Chase ExperimentDoes RNA move from nucleus to cytoplasm?
Chase
UU
UU
U
UU
U
UU
UUU
U
RNA
Uracil
Comparison of artificial RNA polymer experiment & amino acid swap experiment
Artificial polymer Amino acid swap
Need ribosomes, tRNA, tRNA synthetases Cell extract Cell extract
Need to focus on one amino acid
Label each aa, one at a time Label cysteine
Need artificial polymer Use poly(U,G) Use poly(U,G)
Isolate new protein Precipitate, filter Precipitate, filter
Experimental need
Comparison of artificial RNA polymer experiment & amino acid swap experiment
Artificial polymer Amino acid swap
Need ribosomes, tRNA, tRNA synthetases Cell extract Cell extract
Need to focus on one amino acid
Label each aa, one at a time Label cysteine
Need artificial polymer Use poly(U,G) Use poly(U,G)
Isolate new protein Precipitate, filter Precipitate, filter
Experimental need
Alter amino acid on tRNA ---
Convert cysteine to alanine
Classes of RNA and Why they are
Table 1. Comparison of classes of RNA in a typical bacterium (E. coli)Class Types Fraction Sizes Stability Function
Ribosomal RNA(rRNA)
3 80%2904bp,
1542bp, 120bpstable
Scaffold for ribosomal proteins,participates in translation
Transfer RNA(tRNA)
37 12% 76bp to 91 bp stable Connects codon to amino acid
Messenger RNA(mRNA)
1000’s 5%~300 bp to~10,000 bp
unstableCarries information on amino acidsequence of protein
