genetic engineering. clip clips
DESCRIPTION
Steps Isolating the gene of interest Building a cloning construct [Gene targeting] Transformation SelectionTRANSCRIPT
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Genetic Engineering
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• Clip• http://www.bbc.co.uk/programmes/
b00plcl4#clips
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Steps
• Isolating the gene of interest• Building a cloning construct• [Gene targeting]• Transformation• Selection
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Step 1: Culturing the gene of interest
• Animation• Ecoli• Heat shock
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Eukaryotic vs. Plasmid DNA
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Restriction Enzymes
• Endonucleases• Creating “sticky ends”• Like puzzle pieces
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Cloning Vector
• Restriction enzyme sites as part of a multiple cloning site
• Resistance marker or antibiotic resistance marker
• Promoter
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Cloning Methods
• Plasmid• Bacteriophage
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Bioethics Concerns
• The Future of Food• Monsanto• Patents
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DIY Bio and
Biobricks
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Agrobacterium tumefaciens
• Agrobacterium• Gene gun• Horizontal gene transfer
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Three Vehicles
E. coliDNA construction chassis
Agrobacterium tumefaciensDNA transfer chassis
Arabidopsis thaliana
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Applications in Research: Fluorescence
• Tracking experiments• Expression studies
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Brainbow
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Knock-out andKnock-in Libraries
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molecular gastronomythe sweet science
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brazzein protein is 2000X sweeter than sugar
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miraculin is a flavor inverter
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Golden Rice
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Vitamin A deficiency
• http://goldenrice.org/Content3-Why/why1_vad.html
• http://ucbiotech.org/resources/presentations/new_talks/shakespear/shakespeare2.html
• http://www.scidev.net/en/opinions/golden-gm-rice-shines-again.html
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Genetically Engineered Viruses
• http://web.mit.edu/newsoffice/2009/virus-battery-0402.html
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• RNAi?• PCR and extracting genes• Lac operon