genetic analyzer: abi sequence analysis...
TRANSCRIPT
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FVCC:BioTech Morrison 1/25/2011 Page 1
Genetic Analyzer: ABI
Sequence Analysis Module
Prepared by: Bob Morrison
STLCC- FV, Instrumentation Specialist
Initiated: Sep 10, Last Update Dec 10 2010
ABI 3500 Genetic Analyzer
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Genetic Analyzer: Applied Biosystems Information
FVCC:BioTech_Morrison 1/25/2011 Page 2
Online Information and Worldwide Sites: www.appliedbiosystems.com/
Online Support and Information: www.appliedbiosystems.com/support/
Online Computer Session Support: abi.webex.com/
Equipment Information
Instrument Model: 3500 (ex: 3500 or 7900 or SOLID)
Instrument Serial Number: 22117-071 (ex: 2009XXX)
Computer Mfr Model: Optiplex 960 (ex: OptiplexTM 960 or Latitude E6500)
Computer Mfr Serial Number (SN): Service Tag H26PVL1 (ex: FMWXXXX)
Other Equipment & SN (as applicable): Xress Svc. Code: 37137517093 (
Software/Firmware Information
Computer Operating System & SP: Vista SP2 (ex: Win XP Pro SP3 or Vista Bus SP1)
Instrument Software & Version: DC1.0 (ex: Data Collection ver 3.0)
Other Software/Firmware & Version: GM4.1 (ex: Genepmapper 4.0)
Other Software/Firmware & Version: Seq-A 5.4 (ex: Seq Analysis v5.3)
Other Software/Firmware & Version: VR1.1 (ex: SDS 1.2.3)
Products & Service: 800-831-6844
Field Service Engineer: John Buck
Link to ABI 3500 User Manual…pdf (18mb)
Link to Sequencing Protocol from BB …pdf
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GA: Select Seq A Icon, Login , then Sample Manager
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SA: Select Protocol for Analysis
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SA; Add Samples to Analyze
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Select Sample from Run, (may need to define path for locating
these)
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Select Add Selected Samples, then Review list
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Select Green Arrow to Start Analysis
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Results of Analysis
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Analysis Report Manager Summary
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Show column “2” and Sequence Tab Selected
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Electropherogram tab selected
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Analysis Report for Well 2