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Gene expression and function analysis sample and assay technologies by QIAGEN Enjoy first-time success Rely on QIAGEN’s manual and automated workflow solutions for: Sample collection and disruption RNA stabilization and purification Real-time PCR and RT-PCR and gene expression assays RNAi and gene silencing miRNA purification and assays Methylation analysis in epigenetics research Protein sample preparation and assays Making improvements in life possible — www .qiagen.com Sample & Assay Technologies GEFPortf0309S1WW

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Page 1: Gene expression and function analysis sample and …...Gene expression and function analysis sample and assay technologies by QIAGEN Enjoy first-time success Rely on QIAGEN’s manual

Gene expression and function analysis

sample and assay technologies by QIAGEN

Enjoy first-time success

Rely on QIAGEN’s manual and automated workflow solutions for:

■ Sample collection and disruption

■ RNA stabilization and purification

■ Real-time PCR and RT-PCR and gene expression assays

■ RNAi and gene silencing

■ miRNA purification and assays

■ Methylation analysis in epigenetics research

■ Protein sample preparation and assays

Making improvements in life possible — www.qiagen.com

Sample & Assay Technologies

GEFPortf0

309S1W

W

Page 2: Gene expression and function analysis sample and …...Gene expression and function analysis sample and assay technologies by QIAGEN Enjoy first-time success Rely on QIAGEN’s manual

For full access toScience Classic, become a AAAS/Sciencemember

at AAAS.org/join or recommendScience Classic to your librarian at

http://sciencemag.org/cgi/recommend_subscription

On July 20, 1969, Neil Armstrong and Edwin “Buzz” Aldrin achieved one of themost significant scientific breakthroughs of the 20th century by becoming thefirst men to walk on the moon.

In 1970, Science published a special issue devoted to this remarkable achieve-ment, filled with analyses of the lunar samples the astronauts brought homeand experiments they conducted on the moon.

In honor of the 40th anniversary of this historic event, Science is opening itsonline archives, Science Classic, to make this commemorative issue free withregistration.

To get your free access visit Science Classic online at:

www.sciencemag.org/apollo11

From the Science Archives: Apollo 11

Page 3: Gene expression and function analysis sample and …...Gene expression and function analysis sample and assay technologies by QIAGEN Enjoy first-time success Rely on QIAGEN’s manual

GE Healthcare

© 2009 General Electric Company – All rights reserved.GE Healthcare Biacore AB, a General Electric Company.

Biacore systems

from inspiration...to publication

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Page 4: Gene expression and function analysis sample and …...Gene expression and function analysis sample and assay technologies by QIAGEN Enjoy first-time success Rely on QIAGEN’s manual

sigma-aldrich.com

Sigma and Sigma-Aldrich are registered trademarks belonging to Sigma-Aldrich Co. and its affiliate Sigma-Aldrich Biotechnology, L.P.

CompoZr is a trademark of Sigma-Aldrich Co. and its affiliate Sigma-Aldrich Biotechnology, L.P.

the Gene Knockout YouAlways Wanted

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Page 5: Gene expression and function analysis sample and …...Gene expression and function analysis sample and assay technologies by QIAGEN Enjoy first-time success Rely on QIAGEN’s manual

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CLONING & MAPPINGDNA AMPLIFICATION

& PCRRNA ANALYSIS

PROTEIN EXPRESSION &

ANALYSIS

GENE EXPRESSION

& CELLULAR ANALYSIS

EXCEPTIONAL QUALITY

Restriction Enzymes from New England BiolabsWith 35 years of experience in enzyme technology, New England Biolabs leads the industry in quality, selection and innovation for

restriction enzymes. Our expertise in purification coupled with a reliance on recombinant technology results in highly pure enzymes

suitable for even the most sensitive applications. Additionally, stringent quality controls ensure that our enzymes deliver optimal

performance. For quality you can count on, make NEB restriction enzymes your first choice.

Power and purity of NEB Restriction Enzymes

Recombinant HindIII is powerful enough to digest 1 µg of DNA in 5 minutes, but

can also be used in overnight digests with no indication of nuclease contamination.

Marker (M) is the 1 kb DNA Ladder (NEB #N3232).

r Choose recombinant enzymes for a higher quality product with lot-to-lot consistency

0 5 15 30 60 0/N M

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Page 6: Gene expression and function analysis sample and …...Gene expression and function analysis sample and assay technologies by QIAGEN Enjoy first-time success Rely on QIAGEN’s manual

Healthy cell cultures are the bedrock of biologicalresearch. And Stemgent knows that the hardestcells to maintain properly are stem cells. Yourability to keep your stem cells healthy, in anef�cient and affordable manner, requires mediathat are consistent, reliable, and completely free ofcontaminants. That’s why we’ve introduced a newline of Stemedia™: the Stemgent way to culturestem cells.

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❑ Support long-term culture of stem cells.

❑ Enhance every step in cell culture.

Stemedia™. Rationally Designed.Affordable.

Teratoma Formation hESCs (H1) cultured with NutriStem™for 14 passages were injected subcutaneously into SCID mice.Teratomas were harvested after 8 weeks and processed forhistological analysis. Tissue types from all three germ layerscould be identi�ed in the sections (A–C). (A) ectoderm withneural rosette (arrow marked N); (B) endoderm with columnarepithelium (arrow marked E); (C) mesoderm with cartilage(arrow marked C).

The First Stemedia Product Available Now:NutriStem™XF/FF Culture Medium

The �rst medium in the line, NutriStem™ XF/FFCulture Medium, is the only xeno-free humanembryonic stem cell culture medium on the market.

❑ A proprietary, animal component-free, serum-free

medium formulation eliminates risk of animal virus

contamination.

❑ Low bFGF-formulation reduces cost and allows you

to better control your cell culture system.

❑ NutriStem™ XF/FF Medium is a complete, ready-

to-use formulation (containing Alanyl-glutamine).

❑ Supports expansion of hES cells in feeder-free

culture conditions (on Matrigel™) or on feeder layers

(human foreskin �broblasts, MEFs).

Morphology hESC (H1) morphology at passage 5 in NutriStem™

Q-PCR

Q-PCR levels of gene expression %GAPDH Passage 2, Matrigel™

Get to the precise information you need faster. When you see one of these link bars, go to

Stemgent.com and add the /xxxx extension. It’s a direct link to the info you’re looking for.

Introducing Stemedia�.Culture Stem Cells

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OCT NANOG FGF4

%

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Competitor A

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Proliferation I. hESCs (H1, passage 6) were seeded in 96-well plates(Matrigel™ coated) in the different media. Media were changed every24 hours. The number of cells was determined using CyQuant™ cellproliferation assay kit. II. Evaluation of NutriStem™ hESCs (H9.2) usingMatrigel™ as a matrix. Growth promotion for hESCs cultured inNutriStem™ versus competitor A. Cell counts are reported for days 2,4, and 7.

0.00

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24 48 72 96 120 144

NutriStem� XF/FF

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Day 2 Day 4 Day 7

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Page 7: Gene expression and function analysis sample and …...Gene expression and function analysis sample and assay technologies by QIAGEN Enjoy first-time success Rely on QIAGEN’s manual

Our purpose is to move stem cell science forward.

To that end, two things are required. First we

need to work with the world class scientists

responsible for the breakthroughs that make stem

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OurMission.

DAPI Phase SSEA4 OCT4

Immunostaining hESCs (H1) show expected morphology andimmuno- �uorescence staining of pluripotency markers

©2009 by Stemgent, Inc. Stemgent, Reprogramming the Reagent, the What’s Next logo-type, Stemedia and NutriStem are trademarks of Stemgent, Inc. Matrigel is a trademarkof BD Biosciences. CyQuant is a trademark of Molecular Probes. All rights reserved.

SSEA4 expression: hESCs (H1) were cultured in different mediafor 6 passages.

OCT4 expression: hESCs (H1) were cultured in different mediafor 6 passages.

Gene Expression Analysis by Flow Cytometry

Stemgent is committed to producing a completesystem of cell culture products, including media,substrates, cytokines, and enzymes.Our scienti�c advisory board represents a uniqueassociation of some of the most accomplished mindsin stem cell science. Our work is not only inspired byour board, but everything we produce must maintaintheir high standards through every step of production,delivery, and support.

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Stemgent Raises the Bar For HumanEmbryonic Stem Cell Media.

Embryoid Body Formation hESCs (H9.2) were cultured with NutriStem™using Matrigel™ matrix for 16 passages and tested in vitro for pluripotencyby EB formation. After being transferred to suspension culture using serum-supplemented medium, the cells spontaneously formed EBs containingcells representing embryonic germ layers. Histological sections of 14-dayold EBs showed the following cell types: (A) neural rosette (ectoderm),(B) neural rosette stained with anti-tubulin, (C) primitive blood vessels(mesoderm) and (D) megakaryocytes (mesoderm). H&E stain.

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Page 8: Gene expression and function analysis sample and …...Gene expression and function analysis sample and assay technologies by QIAGEN Enjoy first-time success Rely on QIAGEN’s manual

I know you’ll like

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Some discounts, coverages, payment plans, and features are not available in all states or in all GEICO companies. Discount amount varies in some states. Discount is not available in all states or in all GEICO companies. Onegroup discount applicable per policy. In New York a premium reduction is available. AAAS is compensated for allowing GEICO to offer this auto insurance program to AAAS members. GEICO auto insurance is not available in

Mass. Government Employees Insurance Co.● GEICO General Insurance Co.●GEICO Indemnity Co.●GEICO Casualty Co. These companies are subsidiaries of Berkshire Hathaway Inc. GEICO:Washington, DC 20076. GEICOGecko image © 1999 – 2008. © 2008 GEICO

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High PerformanceAntibodies ForHigh Credibility Characterizations.

Stemgent Small Molecules.Essential.

–Sheng Ding, Ph.D.Associate Professor of Chemistry,

Scripps Research InstituteMember, Stemgent Advisory Board

© 2009 by Stemgent, Inc. Stemgent, Reprogramming the Reagent and the What‘s Next logotypeare trademarks of Stemgent, Inc. All rights reserved.

“Recent reports have shown that

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BOSTON|www.stemgent.com/sci1|SAN DIEGO

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Page 9: Gene expression and function analysis sample and …...Gene expression and function analysis sample and assay technologies by QIAGEN Enjoy first-time success Rely on QIAGEN’s manual

New Products

LIFE SCIENCE TECHNOLOGIES AAAS/Science Business Office

Newly offered instrumentation, apparatus, and laboratory materials of interest to researchers in all disciplines in academic, industrial, and governmental organizationsare featured in this space. Emphasis is given to purpose, chief characteristics, and availabilty of products and materials. Endorsement by Science or AAAS of anyproducts or materials mentioned is not implied. Additional information may be obtained from the manufacturer or supplier.

Electronically submit your new product description or product literature information! Go to www.sciencemag.org/products/newproducts.dtl for more information.

494 24 JULY 2009 VOL 325 SCIENCE www.sciencemag.org/products

Microfluidics Chip FunctionalityDolomite now offers the ability to integrate metal electrodes into its range of glass andpolymer microfluidic chips. A wide variety of geometries can be achieved, such as inter-digitated electrodes, heaters, and temperature sensors. Applications include impedancedetection, temperature control in microreactors, and generation of electrical fields for di-electrophoresis. With its excellent chemical-resistant and heat-resistant properties, plati-num is most frequently used as an electrode material, but a wide range of metals can bedeposited onto the microfluidic glass or polymer chips, including gold, copper, titanium,and chrome. Metal feature size can be as small as 2 µm. Metal layers can also be depositedon the inside surface of the microchannels.

DolomiteFor information +44-1763-242491www.dolomite-microfluidics.com

Desktop NanofabricationThe next generation Dip PenNanolithography (DPN) system, the DPN5000, brings greater control and performance to desktop nanofab-rication. The DPN 5000 offers versatile nanopatterning capabilitiescoupled with high-performance atomic force microscopy imaging forimmediate characterization of the deposited patterns. System high-lights include an ultralow-noise scanner with closed-loop flexuretechnology to produce accurate and repeatable nanoscale patterns.For subsequent imaging of substrates, a low-coherence laser witha reduced laser spot size ensures high-quality lateral-force imag-ing. Enhanced lithography software, InkCAD 4.0, includes improvedcontrol of tip-based patterning, along with nanoscale mapping andpositioning, allowing users to precisely position multiple features,even those created from different materials.NanoInkFor information 847-679-6266www.nanoink.net

Sperm Freezing KitThe JAX Sperm Cryo Kit provides a reliable and cost-effective methodof cryopreserving the sperm of novel mouse models. Researcherscan preserve the sperm in their own laboratories, while still enjoyingthe benefit of having The Jackson Laboratory perform quality con-trol testing and provide long-term storage. The kit contains all thenecessary items, including collection dishes, straws and cassettes,freezing apparatus, cryo-protective media, and a detailed instruc-tion manual. It even includes extra reagents and plasticware to per-form a practice run. The Jackson Laboratory will perform a qualitycontrol test on one sperm sample from each strain to assess fertilityand report the results back.The Jackson LaboratoryFor information 800-422-6423www.jax.org/jaxservices

Confocal FRAP ImagingThe VT-HAWK combines high precision point-scanning fluorescencerecovery after photobleaching (FRAP) with multibeam confocalimaging. The instrument’s ability to switch between imaging andphoto-bleach modes in seconds enables accurate quantitation offluorescence intensity dynamics in precise, user-defined regionsof the image. VT-HAWK supports FRAP, fluorescence loss in photo-

bleaching, photo-activation, and photo-conversion techniques withselectable pinhole sizes for confocal imaging. The instrument’s op-tics deliver optimal laser beam quality at the sample without com-promising the performance of either mode. Options include a laser-merge module that houses up to six solid-state lasers.VisiTech InternationalFor information +44-(0)-191-5166255www.visitech.co.uk

Metabolite DatabaseThe Symyx Metabolite database, a comprehensive collection of drugmetabolism data, is now available to pharmaceutical, agrochemical,and biotechnology researchers via the Symyx Isentris data access,analysis, and decision support system, as well as the web-basedDiscoveryGate content platform. The database can accelerate re-search and development productivity by making it possible for sci-entists to focus on the most therapeutically promising compounds(those with the right metabolic profile) early in the discovery pro-cess. Scientists can use Symyx Metabolite’s structure-searchablemetabolic schemes, biotransformation explorer, and easy-to-usequery and browse tools to explore the fate of parent compounds,their metabolites, and properties by leveraging in-house and Symyxdatabase information.Symyx TechnologiesFor information +44-208-546-0869www.symyx.com

Electrophoresis SoftwareSpresso is free, open-source software for electrophoresis that canease experimental design and troubleshooting. The software pro-vides a new tool for designing and optimizing complex electrophore-sis problems. The designers say that the software is 75 times fasterthan current tools thanks to unique numerical algorithms. The speedcan mean the difference between waiting overnight to get a resultand getting it in a few minutes. Spresso allows scientists to iteratethrough experimental variations much faster than they could by go-ing into the lab and relying on trial and error.Stanford School of EngineeringFor information 650-736-2245microfluidics.stanford.edu/spresso/2008/09/introducing-spresso.html

New Products

Page 10: Gene expression and function analysis sample and …...Gene expression and function analysis sample and assay technologies by QIAGEN Enjoy first-time success Rely on QIAGEN’s manual

“It’s truly exhilarating tobe recognized for yourhard work. This prizewill motivate younggenerations of scientistsand encourage newdiscoveries.“

Submission

deadline

August 1

imagination at work

For the purpose of this prize, molecular biology isdefined as “that part of biology which attempts tointerpret biological events in terms of the physico-chemical properties of molecules in a cell”.

(McGraw-Hill Dictionary of Scientific andTechnical Terms, 4th Edition).

GE Healthcare Bio-Sciences AB, a GeneralElectric Company.Björkgatan 30, 751 84 Uppsala, Sweden.© 2009 General Electric Company– All rights reserved.

The GE & Science Prize for Young Life Scientists.

Rewarding brilliance since 1995.

Imagine standing on the podium at the Grand Hotel in Stockholm, making youracceptance speech. Imagine joining the ranks of those published in Science magazineand having your essay on your work in molecular biology read by your peers aroundthe world. Imagine taking part in a seminar with the other Prize winners and NobelPrize laureates and discussing your work with leaders in the field. Imagine what youcould do with the US$25,000 prize money. Imagine this brilliant start to your careerand where it could lead you. Now stop imagining, and make it a reality.

Dr. Christine Jacobs-Wagner did just that. In 1997, she entered in the GE & SciencePrize for Young Life Scientists and won the grand prize. So why not you? If you are oneof those who were awarded a Ph.D in 2008, then submit your 1000-word essay byAugust 1, 2009. Your brilliant idea could well be on its way to building a better reality,for you and for those around you.

All the information you need awaits you at: www.gelifesciences.com/science

Dr. Christine Jacobs-Wagner

Grand Prize winner 1997

Page 11: Gene expression and function analysis sample and …...Gene expression and function analysis sample and assay technologies by QIAGEN Enjoy first-time success Rely on QIAGEN’s manual

For research use only. Not intended for any animal or human therapeutic or diagnostic use, unless otherwise stated.

©2009 Life Technologies Corporation. All rights reserved. The trademarksmentioned herein are the property of Life Technologies Corporation or their respective owners. TaqMan is a registered trademark of RocheMolecular

Systems, Inc. These productsmay be covered by one ormore Limited Use Label Licenses (see the Invitrogen catalog or our website, www.invitrogen.com). By use of these products you accept the terms and conditions of all

applicable Limited Use Label Licenses. A-085988 0609

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