gene expression and cell differentiation
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Gene Expression and Cell Differentiation. CSCOPE Unit: 08 Lesson: 01. There are hundreds of different types of cells in your body, and each type has a unique function. We’re going to compare some different types of cells to see how much they have in common. Your Best Guess. - PowerPoint PPT PresentationTRANSCRIPT
There are hundreds of different types of cells in your body, and
each type has a unique function. We’re going to compare some different types of cells to see
how much they have in common.
Your Best Guess
For each pair of cells in your body, you are going to predict what
percentage of DNA is the same in the two types of cells. Record your
prediction in your science notebooks.
same in your blood cells and nerve cells?
What percentage of the DNA is the
Images Courtesy of Wikimedia Commons
What percentage of the DNA is the
same in your rods (eye) and lung cells?
Images Courtesy of Wikimedia Commons
What percentage of the DNA is the
same in your liver and bone cells?
Images Courtesy of Wikimedia Commons
same in your blood cells and nerve cells?
What percentage of the DNA is the
Answer: 100%
Images Courtesy of Wikimedia Commons
What percentage of the DNA is the
same in your rods (eye) and lung cells?
Answer: 100%
Images Courtesy of Wikimedia Commons
What percentage of the DNA is the
same in your liver and bone cells?
Answer: 100%
Images Courtesy of Wikimedia Commons
Hmmm…
How can these cells, that have very different
functions, have the exact same DNA in the
nucleus?
Taking a Step Back…
HOW does DNA specify for traits in an organism?
HOW does DNA tell cells what to do?
Animations
http://www.dnatube.com/video/2933/The-Human-Genome-Project-Video--3D-Animation-Introduction
http://www.dnalc.org/resources/3d/10-triplet-code.html
http://www.dnalc.org/resources/3d/09-how-much-dna-codes-for-protein.html
Carrying Information in DNA
Core Concepts
DNA triplets code for one amino acid.
Amino acids link together to form polypeptides.
Genes code for polypeptides that control things such as: The expression traits (how we look) The function of the cell Other genes
A very small percentage of the human genome actually codes for proteins.
Now, we are going to do some activities that will help you better understand:
1. Gene expression2. How gene expression is regulated3. How regulation of gene expression causes cells to become different4. How environmental factors play a roll in cell differentiation
What Does Gene Expression Mean?
Let’s look at different ways it is regulated.(Graphic courtesy of Marianne Dobrovolny)
Different Cells Have Different Function…
How?
Use the link below to watch the NOVA video “Epigenetics”.
http://video.pbs.org/video/1525107473#
Epigenetics NOVA Video
What causes cells with the same DNA to differentiate?
What is one way to turn genes on and off?
What causes changes in epigenetics? For example, why do identical twins continue to become more different in terms of their epigenetics as they get older?
Gene Regulation: Modification of Genome
(DNA)
Sections of DNA called transposons can be moved to different chromosomes.
Chemical factors can structurally change the DNA, turning it on or off. Chemical processes: DNA methylation & histone
modification Epigenome
Gene regulation at the DNA level happens in eukaryotes and rarely, if ever, in prokaryotes.
Epigenome
The term epigenome is derived from the Greek word epi, which literally means "above" the genome.
The epigenome consists of chemical compounds that modify, or mark, the genome in a way that tells it what to do, where to do it, and when to do it (i.e., turn genes on and off)
Different cells have different epigenetic marks.
The environment causes changes in our epigenetics.
In a fetus, cells with the exact same DNA
are directed to differentiate by
chemical signals that cause certain genes to be switched on or
off in.
Cell Differentiation
Gene Regulation: Transcriptional Regulation
Transcriptional factors (regulatory proteins) turn transcription on and off or increase and decrease.
This mechanisms happens in eukaryotes and prokaryotes.
Prokaryotes: Transcriptional Regulation
Operon – functioning unit of DNA containing the following: A set of genes (DNA the codes for mRNA) Regulatory sections (DNA that controls the
expression of the gene)
Eukaryote:Transcriptional Regulation
Much more complicated and involves: Many regulatory proteins (transcription
factors) Enhancers and TATA Box
One gene can result in several different proteins through a process called:
Alternate mRNA Splicing
Posttranscriptional Control:mRNA Processing
Posttranscriptional Control:Non-Coding RNA
IMPORTANT: Not all of our DNA codes for mRNA that then translate into proteins!!!
Some DNA codes for non-coding RNA (ncRNA). This ncRNA plays a very important role in gene expression.
Some examples of ncRNA you are familiar with and others you are not: tRNA – helps in translation rRNA – helps in translation miroRNA – prevents translation from happening siRNA – destroys mRNA molecules snRNA – helps splice exons together during mRNA processing See a more complete list here:
http://www.newworldencyclopedia.org/entry/RNA#List_of_RNA_types
Translational Regulation:
These mechanisms prevent the synthesis of proteins.
Example: Regulatory proteins bind to specific
sequences in the mRNA and prevent ribosomes from attaching.
Happens in eukaryotes and prokaryotes
Protein Modification:
Proteins are chemically modified (ex. folded ) after they are made.
These chemical mechanisms can cause the folding process of proteins to change therefore altering how that protein will be expressed.
DNA Microarrays
This technology helps scientists understand the differences in different types of cells, despite the fact that they have the exact same DNA.
Images Courtesy of Wikimedia Commons
In Your Science Notebooks, Complete the Following
Sentence:
DNA microarrays help scientists study the human genome by…
Sources
National Institutes of Health. National Human Genome Research Institute. “Talking Glossary of Genetic Terms.” Retrieved October 16, 2011, from http://www.genome.gov/glossary/ http://www.genome.gov/glossary/?id=167
Images Courtesy of Wikimedia Commons
NOVA scienceNOW. “Epigenetics.” Retrieved September 16, 2012 from http://video.pbs.org/video/1525107473#