Fig. 3. Effect of different extraction temperature on the recoveries of (A) GA and(B) VA by MAE with extraction time: 20 min.

GA remained stable up to 100 °C while the yield of VA decreased at 120 °C.

Fig. 4. Effect of extraction time on the recoveries of (A) GA and (B) VA by MAE at extraction temperature: 100 ◦C.

The optimized MAE conditions for VA were at 100 °C and 10 min.

The optimized MAE conditions for GA were at 100 °C and 20 min.

Table 2. Comparison of amount of GA and VA extracted from different batches of G. elata Blume using MAE and heating under reflux with water as extraction fluid

The extraction efficiency of MAE was comparatively higher than heating under reflux of GA at 100 °C for two different batches of botanicals.

A longer extraction time would result in lower yield for VA.

MAE can save a lot of time for the extraction of many samples.

Fig. 5. Chromatograms obtained for GA and VA in GE by (A) PHWE at 120 ◦C and (B) MAE at 100 ◦C

Both methods had demonstrated to be efficient in the extraction of GA and VA .

MAE showed better extraction efficiency for GA compared to PHWE

Table 2

Table 1

Conclusions

PHWE and MAE showed that thermally labile

compounds could be extracted at an optimized elevated

temperature using pure water as extraction solvent

without the addition of organic modifier/solvent.

Conclusions

The extraction efficiency of PHWE and MAE was

found to be comparable or higher compared to heating

under reflux.

PHWE and MAE methods were shown to be feasible

alternatives for the extraction of thermally labile

marker compounds present in medicinal plants.

Thank you for your attention

HPLC

移動相儲瓶、高壓幫浦、注射閥、層析管柱 C18 RP(25 cm×I.D 4.6 mm) 或同級品。紫外光偵測器 ( 使用 270 nm) 、數據處理系統或記錄器

移動相儲瓶

幫浦

注射閥

層析管柱

紫外光偵測器

數據處理系統

記錄器廢液

參考文獻：(1)尚偉芬等 . 天麻藥理作用研究發展 . 中草藥 28 (10)： 629-632

(1997).

(2)吳惠平等 . 天麻對實驗性癲癇豚鼠內兒茶酚胺含量的影響 .

南京中醫學院學報 6 (2)： 49 (1990).

(3)莫云強 . 雲南植物研究 1 (2)： 230 (1980).

(4)趙國舉等 . 人栽與野生天麻中樞藥理作用的比較研究 .

武漢醫學院學報 9(1)： 91 (1980).

(5)石漢基 , 涂普文 , 胡家才等：愈風湯天麻湯防治缺血性中風基理的實驗研究 .

湖北中醫雜誌 17 (2)： 51 (1995).

(6)于順龍等 . 天麻抗炎效果研究 20 (5)： 19-21 (1989).

(7)寒冬等 . 黃耆、天麻、首烏的延緩衰老作用的實驗研究 .

錦州醫學院報 12(4)： 171 (1991).

(8) 楊菁等 . 黃耆、天麻對老年心藏血管疾病患者血中 SOD 的影響 .

錦州醫學院報 16 (1)： 15 (1995).

(9)Chu-Rei Wu, Ming-Tsuen Hsieh and Jen Liao. ρ-Hydroxybenzyl Alcohol

Attenuates Learning Deficits in the Inhibitory Avoidance Task.

Involvement of Serotonergic and Dopaminergic,Chinese Journal of

Physiology 39 (4)： 265-273(1996).156

(10)Ming-Tsuen Hsieh, Chu-Rei Wu and Chieh-Fu Chen. Gastrodin and

ρ-Hydroxybenzyl alcohol facilitate memory consolidation and

retrieval, but not acquisition, on the passive avoidance

task in rats. Journal of Ethnopharmacology 56 (4) ： 45-54 (1997)