G-FectinTM

1. A novel transfection reagent developed by Genolution

2. Cationic lipid based 3. no cell splitting is required 4. Whole transfection can be done in 10 min5. Your gene silencing in 24 hours6. Lower toxicity than other reagents7. Better gene silencing8. For 500X transfection to one well of 24 well plate

G-Fectin has good Transfection efficacy

Transfection assay using Vimentin siRNA final 10 nM

G-Fectin has low cytotoxicity

Cell v

iability

%

All described cells were spitted in 30 % confluency in 24h ahead of viable cell counting with treatment of lipo-plexed RNA.

10 min transfection 10 min transfection protocol protocol

with G-Fectinwith G-Fectin50 ul of

PBS

1-ul ofG-factinGenolution siRNA

Add to 24 well plate directly Cells

seed on plate

Incubate 24 hrs

qPCR

Add the reagent & siRNA to the

PBS

Incubate 10 min at RT

450 ul of Suspended cells

(5 x 104 cells/ well)

siRNA Transfection assay kit

• G-Fectin KitTM

–The easiest and fastest way for siRNA transfection assay test

– Just add to the cell, wait for 48hours, and count the viable cell– The target gene of the used siRNA is essential for cell viability– The cell toxicity and level of the gene silencing is proportional

Materials included

scramble siRNA

control siRNA (cause to cell death if transfected, for 200X assay )

siRNA dissolving buffer

G-factin 0.5ml (for 500 X transfection to 24 well)

Easy siRNA Transfection assay kit

Cell viability in 48 hour

Scramble siRNA target siRNA

Gene silencing in 24 hour

Materials included scramble siRNAcontrol siRNA (cause to cell death if transfected )siRNA dissolving bufferG-factin 0.5ml (for 500 X transfection to 24 well)