f.y.b.sc....mordant and fixative. 3.5 natural and synthetic dyes. 3.6 simple staining, differential...

B. K. Birla College (Autonomous), Kalyan

Department of Biotechnology

F.Y.B.Sc.

Syllabus for Autonomy

Year

2018-19

Preamble Twenty First Century is known as the ‘Century of Biotechnology’. Biotechnology is one

of the youngest branches of Life Science, which has expanded and recognized as an

advanced interdisciplinary applied science in last few years. Biotechnology at the core

envisages the comprehensive study of Life. The Interdisciplinary aspect of Biotechnology

has led to a unique and dynamic status for this subject in the field of Research and

Industry.

The socio-economic potential of Biotechnology is well established. Biotechnology has its

applications in almost every field touching practically every human activity. The applied

aspect of Biotechnology is now getting recognized with its applications in various

industries viz. Agriculture, Health, Environment.

Biotechnology demands trained and skilled human resources to establish the Industry and

Research sectors. The field is novel and still expanding which demands inputs in

Infrastructure and Technology. The global and local focus is on developing new

technological applications are fast growing. Biotechnology sector in Research and

Industry is expanding which is set to predict the next major revolution in the world.

The demand for trained workforce in Biotechnology is ever growing in Fundamental

Research and Industry Sector. Academic and Research Sectors also require

interdisciplinary trained manpower to further the Biotechnology Revolution.

The need of the hour is to design appropriate syllabi which keeps pace with changing

times and technology with emphasizes on applications while elucidating technology in

depth. The present Syllabi is restructured anticipating the future needs of Biotechnology

Sector with more emphasis on imparting hands-on skills. The main thrust is laid on

making syllabus compatible with developments in Education, Research and Industrial

sectors. The Theory and Practical course in new restructured course will lead to impart

skill-set essentials to further Biotechnology Sector.

The restructured syllabus combines basic principals of Physical, Chemical and Biological

sciences in light of advancements in technology. The curriculum aims to impart basic

knowledge with emphasis on its applications to make the students industry ready.

F.Y.B.Sc.

SEMESTER I Credits

BUSBT101 Basic Chemistry -I 02

BUSBT102 Biochemistry- I 02

BUSBT103 Basic Life Science-I 02

BUSBT104 Basic Life Science-II 02

BUSBT105 Biotechnology-I 02

BUSBT106 Biotechnology-II 02 BUSBT 107 Ability Enhancement Course 1 02

Practical

BUSBTP101 Basic Chemistry 02

BUSBTP102 Life Sciences 02

BUSBTP103 Biotechnology 02

SEMESTER II

BUSBT201 Basic Chemistry- II 02

BUSBT202 Biochemistry- II 02




BUSBT206 Biotechnology-II 02

BUSBT207 Ability Enhancement Course 2 02

Practical

BUSBTP201 Basic Chemistry 02

BUSBTP202 Life Sciences 02

BUSBTP203 Biotechnology 02

SEMESTER I SEMESTER II

Basic Chemistry -I Basic Chemistry- II

Unit I Nomenclature and classification

of chemical compounds

Thermodynamics

Unit II Chemical Bonds Chemical Kinetics

Unit IIII Water and Buffers Titrimetry and Gravimetry

Biochemistry- I Biochemistry- II

Unit I Carbohydrates Amino acids and proteins

Unit II Lipids Enzymes and Coenzymes

Unit IIII Nucleic acids and genetic

material

Analytical techniques

Basic Life Science-I Basic Life Science-I

Unit I Animal and Plant Diversity Plant Physiology

Unit II Introduction to Microbial

diversity

Animal Physiology

Unit III Classification of Bacteria Classification and cultivation of Viruses

Basic Life Science-II Basic Life Science-II

Unit I Sterilization techniques Prokaryotic Cell Organization

and Division

Unit II Nutrition, cultivation and

enumeration of microorganisms

Eukaryotic Cell Organization and

Division

Unit III Microscopy and stains Ecology: Biogeochemical cycles

Biotechnology-I Biotechnology-I

Unit I Scope of Biotechnology Prokaryotic and Eukaryotic Replication

Unit II Applications in Biotechnology Microbial genetics

Unit III Ecology and interactions Population genetics

Biotechnology-II Biotechnology-II

Unit I Mendelian and Non-Mendelian

inheritance

Model organisms in biology

Unit II Eukaryotic genome and

Chromosomal disorders

Basic Immunology

Unit III Basic Biostatistics DNA mutations and DNA repair

Ability Enhancement Course 1 Ability Enhancement Course 2

SEMESTER I Credits

BUSBT101 Basic Chemistry -I 02

Unit I Nomenclature and classification of chemical

compounds

1.1 Nomenclature and Classification of Inorganic

Compounds: Oxides, Salts, Acids, Bases, Ionic,

Molecular and Coordination Compounds

1.2 Nomenclature and Classification of Organic

Compounds: Alkanes, Alkenes, Alkynes, Cyclic

Hydrocarbons, Aromatic Compounds, Alcohols

and Ethers, Aldehydes and Ketones, Carboxylic

Acids and its derivatives, Amines, Amides, Alkyl

Halides and Heterocylic Compounds

15

Lect

ures

Unit II Chemical Bonds

2.1 Ionic Bond: Nature of Ionic Bond, Structure of NaCl,

KCl and CsCl, factors influencing the formation

of Ionic Bond.

2.2 Covalent Bond: Nature of Covalent Bond,

Structure of CH4, NH3, H2O, Shapes of BeCl2, BF3

2.3 Coordinate Bond: Nature of Coordinate Bond

2.4 Non Covalent Bonds: van Der Waal’s forces:

dipole - dipole, dipole - induced dipole.

2.5 Hydrogen Bond: Theory of Hydrogen Bonding

and Types of Hydrogen Bonding (with examples of

H2O, RCOOH, ROH, Salicylaldehyde, Amides and

Polyamides).

15

Lect

ures

Unit III Water and Buffers

3.1 Properties of Water, Interaction of Water with

Solutes (Polar, Non-Polar, Charged), Non-Polar

Compounds in Water – Change in its Structure

and the H ydrophobic Effect, Role of Water in

Biomolecular Structure and Function and Water

as a Medium for Life

3.2 Solutions: Normality, Molarity, Molality, Mole

fraction, Mole concept, Solubility, Weight ratio,

Volume ratio, Weight to Volume ratio, ppb, ppm,

millimoles, milliequivalents (Numericals expected).

3.3 Primary and Secondary Standards: Preparation of

Standard Solutions, Principle of Volumetric

15

Lect

ures

Analysis.

3.4 Acids and Bases: Lowry-Bronsted and Lewis

Concepts. Strong and Weak Acids and Bases - Ionic

Product of Water - pH,pKa, pKb. Hydrolysis of Salts.

3.5 Buffer solutions –Concept of Buffers, Types of

Buffers, Derivation of Henderson equation for Acidic

and Basic buffers, Buffer action, Buffer capacity

(Numericals expected.) pH of Buffer Solution.

Ref:

University General Chemistry , 1st edition (2000), C.N.

R. Rao, Macmillan Publishers, India ,

Physical Chemistry University for biological sciences,

1st edition, (2005), Chang R., Science Books, USA

Essentials of Physical Chemistry, 24th edition, (2000),

B S Bahl, G D Tuli, Arun Bahl, S. Chand Limited,

India.

Concise Inorganic Chemistry .5th edition (2008),

Author: J. D. Lee, John Wiley & Sons, USA.

Organic Chemistry, 6th edition, (1992), Morrison

Robert Thornton, Pearson Publication, Dorling

Kindersley (India Pvt. Ltd.)

BUSBT102 Biochemistry- I 02

Unit I Carbohydrates

1.1 Classification, Structure and functions

Monosaccharides: D & L Glyceraldehydes,

stereoisomerism, classes of monosaccharaides,

structure of monosaccharaides, Disaccharides:

Structure, source and functions; Polysaccharides:

Homo- and Heteropolysachharides; Storage and

Structural polysaccharides.; Derivatives and

glycoconjugates

1.2 Characteristic reactions, Physical and chemical

properties

1.3 Carbohydrates as informational molecules

1.4 Carbohydrate analysis and glycomics

15

Lect

ures

Unit II Lipids

2.1 Classification, structure and properties of Lipids:

2.2 Fatty acids (saturated and unsaturated fatty acids,

nomenclature), waxes, triacylglycerols,

phospholipids (Lecithin, Cephalin, Plasmalogen),

2.3 Storage lipids and structural lipids

2.4 Lipoproteins: Structure and Function

2.5 Lipid aggregates: Micelles and bilayers, liposomes

2.6 Rancidity, saponification, lipid peroxidation and

15

Lect

ures

hydrogenation of Oils, Tests to check adulteration

of fats and oils,

2.7 Action of Phospholipases

2.8 Lipids as signals, cofactors and pigments

2.9 Lipid analysis: Extraction, separation and

identification of lipids

Unit III Nucleic acids and genetic material

3.1 DNA, RNA.

3.2 Structure of Purine and Pyrimidine Bases,

3.3 Hydrogen Bonding between Nitrogeneous Bases in

DNA,

3.4 Differences between DNA and RNA,

3.5 Structure of Nucleosides, Nucleotides and

Polynucleotides,

3.6 Different form of DNA and RNA.

15

Lect

ures

Ref:

Outlines of Biochemistry: 5th Edition, (2009), Erice

Conn & Paul Stumpf ; John Wiley and Sons, USA

Principles of Biochemistry, 4th edition (1997), Jeffory

Zubey, McGraw-Hill College, USA

Lehninger , Principles of Biochemistry. 5th Edition

(2008), David Nelson & Michael Cox, W.H. Freeman

and company, NY.

Fundamentals of Biochemistry. 3rd Edition (2008),

Donald Voet & Judith Voet , John Wiley and Sons, Inc.

USA

Biochemistry: 7th Edition, (2012), Jeremy Berg,

Lubert Stryer, W.H.Freeman and company, NY


Unit I Animal and Plant Diversity

1.1 Origin of Life, Chemical and Biological Evolution,

1.2 Origin of Eukaryotic Cell.

1.3 Concept of Biodiversity, Taxonomical,

1.4 Ecological and Genetic Diversity & its

Significance

1.5 Introduction to Plant Diversity: Algae, Fungi,

Bryophyta, Pteridophyta, Gymnosperms and

Angiosperms (with one example each)

1.6 Introduction to Animal Diversity: Non-Chordates

and

Chordates {with at least one representative

example.)

15

Lect

ures

Unit II Introduction to Microbial diversity

2.1 Introduction to Microbial Diversity

15

Lect

2.2 Archaebacteria,

2.3 Eubacteria,

2.4 Blue-green Algae,

2.5 Actinomycetes,

2.6 Eumycota Habitats, Examples and Applications.

ures

Unit III Classification of Bacteria

3.1 Bacteria : Classification, Types, Morphology (Size,

Shape and Arrangement)

3.2 Cultivation of Bacteria.

3.3 Reproduction and Growth (Binary Fission,

Conjugation and Endospore formation)

3.4 Growth Kinetics, Isolation and Preservation.

Significance of Bacteria

15

Lect

ures

Ref:

Jordan, E.L. and Verma P.S. 1978, (i) Chordate

Zoology S. Chand & Company Ltd. Ram Nagar. New

Delhi.

Modern Text Book of Zoology: Invertebrates.

R.L.Kotpal. Publisher, Rastogi Publications, 2012.

Devlin R.M. (1983) - Fundamentals of Plant

Physiology (Mac. Millan, New York)

Dutta A.C. (2000) A Classbook of Botany (Oxford

University Press, UK)

Ganguli, Das Dutta (2011) – College Botany Vol I, II

and III (New Central Book Agency, Kolkata)

Ecology – P.S. Verma and Agarwaal- S. Chand

Publications

Microbiology–6th Edition (2006), Pelczar M.J., Chan

E.C.S., Krieg N.R., The McGraw Hill Companies Inc.

NY

Presscott‘s Microbiology, 8th edition (2010), Joanne

M Willey, Joanne Willey, Linda Sherwood, Linda M

Sherwood, Christopher J Woolverton, Chris

Woolverton, McGrawHil Science Enginering, USA

Text book of Medical Microbiology, Anantnarayan

Microbiology- Frobisher

General Principles of Microbiology- Stanier

Fundamental Principles of Bacteriology - A. J. Salle

McGraw Hill


Unit I Sterilization techniques

1.1 Definition : Sterilization and Disinfection.

1.2 Types and Applications Dry Heat, Steam under

pressure, Gases, Radiation and Filteration

15

Lect

ures

1.3 Chemical Agents and their Mode of Action -

Aldehydes, Halogens, Quaternary Ammonium

Compounds, Phenol and Phenolic Compounds,

1.4 Heavy Metals, Alcohol, Dyes, and Detergents

Ideal Disinfectant.

1.5 Examples of Disinfectants and Evaluation of

Disinfectant

Unit II Nutrition, cultivation and enumeration of

microorganisms

2.1 Nutritional Requirements : Carbon, Oxygen,

Hydrogen, Nitrogen, Phosphorus, Sulphur and

Growth Factors.

2.2 Classification of Different Nutritional Types of

Organisms.

2.3 Design and Types of Culture Media. Simple Medium,

Differential, Selective and Enriched Media

2.4 Concept of Isolation and Methods of Isolation. Pure

Culture Techniques

2.5 Growth and Enumeration. Growth Phases, Growth

Curve. Arithmatic Growth and Growth Yield.

Measurement of Growth. Chemostat and Turbidostat

2.6 Enumeration of Microorganisms- Direct and Indirect

Methods

2.7 Preservation of Cultures- Principle and

2.8 Methods. Cryogenic Preservation Advantages and

Limitations

15

Lect

ures

Unit III Microscopy and stains

3.1 Microscope- Simple and Compound: Principle.

Parts, Functions and Applications.

3.2 Dark Field and Phase Contrast Microscope

3.3 Stains and Staining Solutions, Definition of Dye

and Chromogen.

3.4 Structure of Dye and Chromophore. Functions of

Mordant and Fixative.

3.5 Natural and Synthetic Dyes.

3.6 Simple Staining, Differential Staining and Acid Fast

Staining with specific examples

15

Lect

ures

Ref:

Microbiology–6th Edition (2006), Pelczar M.J., Chan

E.C.S., Krieg N.R., The McGraw Hill Companies Inc.

NY

Presscott‘s Microbiology, 8th edition (2010), Joanne M

Willey, Joanne Willey, Linda Sherwood, Linda M

Sherwood, Christopher J Woolverton, Chris

Woolverton, McGrawHil Science Enginering, USA

Text book of Medical Microbiology, Anantnarayan



Fundamental Principles of Bacteriology - A. J. Salle

McGraw Hill


Unit I Scope of Biotechnology

1.1 History & Introduction toBiotechnology

1.2 What is Biotechnology?

1.3 Definition of Biotechnology,

1.4 Traditional and Modern Biotechnology,

1.5 Branches of Biotechnology- Plant, Animal Biotechnology,

Marine Biotechnology, Agriculture, Healthcare, Industrial

Biotechnology, Pharmaceutical Biotechnology,

Environmental Biotechnology.

1.6 Biotechnology Research in India.

1.7 Biotechnology Institutions in India (Public and Private

Sector)

1.8 Biotech Success Stories

1.9 Biotech Policy Initiatives

1.10 Biotechnology in context of Developing World

1.11 Public Perception of Biotechnology

15

Lect

ures

Unit II Applications in Biotechnology

2.1 Applications of Biotechnology in Agriculture : GM

Food, GM Papaya, GM Tomato,

2.2 Fungal and Insect Resistant Plants-BT Crops, BT

Cotton and BT Brinjal. Pros and Cons

2.3 Biotechnological applications in Crop and Livestock

Improvements

2.4 Modifications in Plant Quality-,Golden Rice,

2.5 Molecular Pharming, Plant Based Vaccines

2.6 Ethics in Biotechnology and IPR

15

Lect

ures

Unit III Ecology and interactions

3.1 Ecology and Biogeography. Ecosystems, Definition

and Components, Structure and Function of

Ecosystems.

3.2 Aquatic and Terrestrial Ecosystems,

3.3 Biotic and Abiotic Factors, Trophic Levels, Food

Chain and Food Web,

3.4 Ecological Pyramids (Energy, Biomass and Number)

3.5 Interactions with examples: Commensalism,

Mutualism, Predation and Antibiosis, Parasitism.

15

Lect

ures

Ref:

Biotechnology B.D. Singh.

Biotechnology 3rd Edition S.S. Purohit

Biotechnology ,U.Satyanarayan,Books & Allied

Pvt.Ltd.

Molecular Biotechnology-Principles and Applications

of Recombinant DNA

Technology 3rd Edition Glick B.R., Pasternak J.J.,

Patten C.L


Publications

Biotechnology: Environmental Processes- Rehm and

Reed- Wiley


Unit I Mendelian and Non-Mendelian inheritance

1.1 Introduction to Genetics and three branches of

genetics.

1.2 Mendelian inheritance: Terms, Why Pisum sativum,

Mendel’s experimental technique, Mendel’s Laws of

Heredity: Monohybrid cross, Law of Dominance and

Law of Segregation.

1.3 Dihybrid Cross: Law of Independent Assortment.

Application of Mendel’s Principles.

1.4 Mendel’s Principle in Human genetics.

1.5 Non-Mendelian inheritance: Why Mendel was lucky,

existence of Non-Mendelian genetics.

1.6 Incomplete Dominance and Codominance, Multiple

alleles,

1.7 Effect of Environment on the expression of the

genes, Penetrance and expressivity,

1.8 Essential genes and lethal alleles,

1.9 Epistasis (Dominant and recessive) and

Quantitative inheritance (kernel color in wheat).

15

Lect

ures

Unit II Eukaryotic genome and Chromosomal disorders

2.1 Organization of eukaryotic genetic material: Histone

proteins, non-histone proteins, nucleosome structure

and 10 nm fibre, 30 nm fibre (solenoid model and zig-

zag model) and chromosome.

2.2 Kinds of chromosome: Polytene chromosome and

lampbrush chromosome.

2.3 Introduction to cytogenetics: G-Banding and

Karyotyping, Study of normal male and female

karyotype.

15

Lect

ures

Unit III Basic Biostatistics

3.1 Defination &Importance of Statistics in Biology

3.2 Types of Data, Normal and Frequency Distribution

Representation of Data and Graphs(Bar Diagrams, Pie

Charts and Histogram, Polygon and Curve)

3.3 Types of Population Sampling Measures of Central

Tendency (For Raw,Ungroup & Group Data)

3.4 Mean, Median ,Mode. Measures of Dispersion

3.5 Range, Variance, Coefficient of Variance.

3.6 Standard Derivation.Standard Error.

Ref:

Genetics, (2006) Strickberger MW - (Prentice Hall,

India)

Human Genetics- A. M. Winchester – MacMillan Press

Microbial Genetics- Freifelder –Narosa Publishing

House

Genes XI, 11th edition (2012), Benjamin Lewin,

Publisher - Jones and Barlett Inc. USA

Molecular Biology of the Gene, 6th Edition (2008),

James D. Watson, Pearson Education, Inc. and Dorling

Kindersley Publishing, Inc. USA

Molecular Biology, 5th Edition (2011), Weaver R.,

McGraw Hill Science. USA

Fundamentals of Molecular Biology, (2009), Pal J.K.

and Saroj Ghaskadbi, Oxford University Press.

Molecular Biology: genes to proteins, 4th edition

(2011), Burton E Tropp Jones& Bartlett Learning, USA

Cell and Molecular Biology – De Robertis- Lippincott

Williams& Wilkins

Introductory Biostatistics. 1st edition. (2003), Chap T.

Le. John Wiley, USA

Methods in Biostatistics- B. K. Mahajan –Jaypee

Brothers

PRACTICALS

Semester I

BUSBTP101: Basic Chemistry (Credits: 02) 1. Safety Measures and Practices in Chemistry Laboratory

2. Working and use of a Digital Balance, pH Meter

3. Inorganic qualitative analysis of mixtures (1 mixtures)

4. Dissociation Constant of Weak Acids by Incomplete Titration Method using pH

Meter and determination of Acetic acid in Vinegar by Titrimetric Method

5. Determination of strength of HCl in commercial sample

6. Organic qualitative analysis (Identification of functional group and Melting/boiling

point of any 4 organic compound).

7. Determination of Sodium carbonate and bicarbonate by double indicator

8. Preparation of Standard (Molar, Molal and Normal solutions) and Buffer Solutions

9. Spot test for Carbohydrates, Lipids and Nucleic Acids

10. Estimation of Reducing sugar by DNSA method

11. Estimation of DNA by DPA method and RNA by Orcinol method

12. Determination of acid number of oils

13. Determination of iodine value of oils

14. Staining of Starch grain from potato, pea, wheat and rice

BUSBTP102: Basic Life Science (Credits: 02)

1. Study of animal tissues from permanent slides:-blood, bone marrow, neuron,

connective tissue-spongy, smooth, skeletal and heart muscles.

2. Study of plant tissues from suitable material - parenchyma, sclerenchyma, xylem

and phloem.

3. Study of bacterial morphology by Monochrome staining

4. Gram staining of organism from different samples.

5. Study of bacterial structures by staining (capsule, cell wall, lipid granules and

spore).

6. Study of bacterial Motility –hanging drop and stab culture (Demonstration).

7. Preparation of Media- Nutrient broth and Agar, MacConkey Agar, Sabourauds

Agar

8. Aseptic transfer of sterile Nutrient Agar/St. Nutrient broth.

9. Isolation of Organisms : T-streak, Polygon method

10. Colony Characteristics of Microorganisms,

11. Growth Curve of E. coli

BUSBTP103: Biotechnology (Credits: 02) 1. Assignment- Study of any branch of biotechnology and its applications

2. All Lab equipments-autoclave, hot air oven, centrifuge, incubator, rotary shaker, filter

assembly, LAF, pH meter

3. Study of Interactions Commensalism, Mutualism, Predation and Antibiosis, Parasitism

4. Study of all the biotic and abiotic components of any simple ecosystem- natural pond

or terrestrial ecosystem or human modified ecosystem.

5. Problems on Mendelian Genetics

6. Study of Mitosis using Onion root tips

7. Effect of Colchicines on mitosis.

8. Study of Meiosis

9. Study of Karyotypes – Normal Male and Normal Female

10. Isolation of Pectin

11. Extraction of casein

SEMESTER II Credits

BUSBT201 Basic Chemistry- II 02

Unit I Thermodynamics: 1.1 System, Surrounding, Boundaries Sign

Conventions, State Functions, Internal Energy

and Enthalpy: Significance, examples,

1.2 Laws of Thermodynamics and its Limitations,

Mathematical expression.

1.3 Qualitative discussion of Carnot Cycle for ideal

Gas and Mechanical Efficiency.

1.4 Laws of Thermodynamics as applied to

Biochemical Systems.

1.5 Redox Chemistry: Oxidation number, Rules to

assign oxidation number,

1.6 Balancing redox reactions by Ion exchange

method and Oxidation number method.

15

Lectures

Unit II Chemical Kinetics

2.1 Reaction Kinetics: Rate of Reaction, Rate

Constant, Measurement of Reaction Rates

Order & Molecularity of Reaction,

2.2 Integrated Rate Equation of First and Second

order reactions (with equal initial concentration

of reactants).

2.3 Determination of Order of Reaction by

1) Integration Method

2) Graphical Method

3) Ostwald‘s Isolation Method

4) Half Time Method. (Numericals expected).

15

Lectures

Unit III Titrimetry and Gravimetry

3.1 Titrimetric Analysis: Titration, Titrant, Titrand,

End Point, Equivalence Point, Titration Error,

Indicator, Primary and Secondary Standards,

Characteristics and examples

3.2 Types of Titration –Acid –Base, Redox.

Precipitation, Complexometric Titration. Acid –

15

Lectures

Base Titration.-Strong Acid Vs Strong Base -

Theoretical aspects of Titration Curve and End

Point Evaluation.

3.3 Theory of Acid –Base Indicators, Choice and

Suitability of Indicators.

3.4 Gravimetric Analysis: Solubility and

Precipitation, Factors affecting Solubility,

Nucleation, Particle Size, Crystal Growth,

Colloidal State, Ageing/Digestion of

Precipitate.

3.5 Co-Precipitation and Post-Precipitation.

Washing, Drying and Ignition of Precipitate.

Ref:

University General Chemistry , 1st edition

(2000), C.N. R. Rao, Macmillan Publishers, India

,

Physical Chemistry University for biological

sciences, 1st edition, (2005), Chang R., Science

Books, USA

Essentials of Physical Chemistry, 24th edition,

(2000), B S Bahl, G D Tuli, Arun Bahl, S. Chand

Limited, India.

Concise Inorganic Chemistry .5th edition (2008),

Author: J. D. Lee, John Wiley & Sons, USA.

Organic Chemistry, 6th edition, (1992), Morrison

Robert Thornton, Pearson Publication, Dorling

Kindersley (India Pvt. Ltd.)

BUSBT202 Biochemistry- II 02

Unit I Amino acids and proteins

1.1 Proteins and Amino Acids: Classification,

Preparation and Properties, Isoelectric Point,

Peptide Synthesis

1.2 Proteins: Classification based on Structure and

Functions, Primary Structure, N-terminal (Sanger

and Edmans Method) and C-terminal Analysis

(Enzyme)Reactions of Amino Acids, Sorenson‘s

Titration, Ninhydrin Test.

1.3 Denaturation of protein Structure of Peptides.

1.4 Titration Curve of Amino Acids.

1.5 Concept of Isoelectric pH, Zwitter ion.

Glycoproteins

15

Lectures

Unit II Enzymes, Coenzymes and Vitamins

2.1 Enzymes: Introduction, Nomenclature and

classification. Chemical nature and

15

Lectures

properties. Mechanism of enzyme

action/catalysis (introduction)

2.2 Active site, Isozymes, Ribozymes and

Abzymes

Co factor and Co-enzyme

2.3 Vitamins and Coenzymes: RDA, source,

structure (of Vitamin and Coenzyme) and

functions of:

1 Water soluble Vitamins: Ascorbic acid,

thiamine, riboflavin, folic acid, pyridoxine,

niacin, pantothenic acid, biotin, lipoic , folic

acid and cyanocobalamin.

2 Fat soluble Vitamins: Vitamin A, D, E and K.

Unit III Analytical techniques

3.1 Methods of Separation: Precipitation, Filtration,

Distillation and Solvent Extraction.

3.2 Analytical Techniques: Chromatography:

Definition, Principles, Types

3.3 Introduction to Paper Chromatography, Thin

Layer Chromatography, Column

Chromatography and its Applications.

3.4 Colorimetry: Principle, Beer-Lambert‘s Law,

Measurement of Extinction, Derivation of E =

kcl, Limitations of Beer-Lambart‘s Law, Filter

Selection

15

Lectures

Ref:

Outlines of Biochemistry: 5th Edition, (2009),

Erice Conn & Paul Stumpf ; John Wiley and

Sons, USA

Principles of Biochemistry, 4th edition (1997),

Jeffory Zubey, McGraw-Hill College, USA

Lehninger , Principles of Biochemistry. 5th

Edition (2008), David Nelson & Michael Cox,

W.H. Freeman and company, NY.

Fundamentals of Biochemistry. 3rd Edition

(2008), Donald Voet & Judith Voet , John Wiley

and Sons, Inc. USA

Biochemistry: 7th Edition, (2012), Jeremy Berg,

Lubert Stryer, W.H.Freeman and company, NY

Enzymes: Biochemistry, Biotechnology &

Clinical chemistry, (2001) Palmer Trevor,

Publisher: Horwood Pub. Co., England

Principles and techniques in biochemistry and

molecular biology (2010), Keith

Wilson and John Walker, 7th edition, Cambridge

University Press


Unit I Prokaryotic Cell Organization and Division

1.1 Ultrastructure of Prokaryotic Cell Size, shape and

arrangements in Bacteria. Cell envelopes: Cell

membrane and Cell wall (Gram positive, Gram

negative and Archaebacteria), Slime Layer and

Capsule.

1.2 Cell surface Structures: Pili and fimbriae, Flagella and

Microbial locomotion

1.3 Cytoplasm :Organization of Genetic Material,

plasmids (Features and Classification) , Storage

1.4 Bodies, Endospores (structure, sporulation and

germination)

1.5 Forms of Bacterial cell Division and Growth phases

(introduction)

15

Lectures

Unit II Eukaryotic Cell Organization and Division

2.1 Ultrastructure of Eukaryotic Cell: Structure and

function of: Plasma membrane, plant cell wall,

Nucleus, Mitochondria, Plastids and Chloroplast,

Endoplasmic Reticulum, Golgi Apparatus, Lysosome,

and Ribosomes.

2.2 Comparison of Prokaryotic And Eukaryotic Cells.

2.3 Introduction to Microfilaments, Intermediate

Filaments, and Microtubules. Locomotory

appendages: Cilia And Flagella. Cell division

(Mitosis).

15

Lectures

Unit III Classification of Viruses

3.1 General Characters, Classification- Plant,

Animal and Bacterial Viruses

3.2 Structure of viruses- bacteriophage, animal

viruses- Small pox , Influenza, Plant viruses-

TMV, CMV

3.3 Structure and Characterization of Viruses and

Significance

3.4 Classification and taxonomy.

15

Lectures

Ref:

Microbiology–6th Edition (2006), Pelczar M.J.,

Chan E.C.S., Krieg N.R., The McGraw Hill

Companies Inc. NY

Presscott‘s Microbiology, 8th edition (2010),

Joanne M Willey, Joanne Willey, Linda

Sherwood, Linda M Sherwood, Christopher J

Woolverton, Chris Woolverton, Mc Graw Hill

Science Engineering, USA

Text book of Medical Microbiology,

Anantnarayan



Fundamental Principles of Bacteriology - A. J.

Salle McGraw Hill

Foundations In Microbiology by Talaro and

Talaro Third edition W.C Brown

Cell and Molecular Biology – De Robertis-

Lippincott Williams& Wilkins Cell and

Molecular Biology- Concepts and Experiments—

Karp – Wiley International


Unit I Plant Physiology

1.1 Photosynthesis, Intracellular Organization of

Photosynthetic System.

1.2 Fundamental Reactions of Photosynthesis,

Photosynthetic Pigments, Role of Light. Hill

Reaction and its Significance, Light Reactions,

1.3 Cyclic and Non-Cyclic Photo induced Electron

Flow,

1.4 Energetics of Photosynthesis, Photorespiration,

Dark Phase of Photosynthesis, Calvin Cycle, C-

3, C-4 pathways

1.5 Plant hormones - Auxin ,Gibbrellins,

Cytokinins, Ethylene, Abscissic acid

1.6 Introduction to Secondary Metabolites

15

Lectures

Unit II Animal Physiology

2.1 Physiology of Digestion Movement of Food and

Absorption, Secretory functions of Alimentary

Canal, Digestion and Absorption,assimilation in

Gut of Mammals

2.2 Physiology of Urine Formation and Role of

Kidney in Excretion and Osmoregulation

2.3 Physiology of Respiration, Mechanism of

Respiration, Principles of Gaseous Exchange in

the Blood and Body Fluids

2.4 Blood and Circulation : Blood Composition,

Structure and Function of its Constituents,

2.5 Blood Coagulation and Anti-Coagulants

15

Lectures

Unit III Nutrient Cycle and Biogeochemical Cycles

3.1 Water, Carbon, Oxygen, Nitrogen, Sulphur and

15

Lectures

Phosphorus.

3.2 Components, Significance, Disruption of the

ecosystem due to anthropological activities.

3.3 Concept of - Endangered, Threatened,

Vulnerable, Rare and Extinct species

Ref:

Jordan, E.L. and Verma P.S. 1978, (i) Chordate

Zoology S. Chand & Company Ltd. Ram Nagar.

New Delhi.

Modern Text Book of Zoology: Invertebrates.,

R.L.Kotpal. Publisher, Rastogi Publications,

2012.

Guyton, Text book of Medical Physiology

Concise Medical Physiology- Sujit K Chaudhari

Human Physiology- Guyton –International

Edition

Human Anatomy- Marieb

Devlin R.M. (1983) - Fundamentals of Plant

Physiology (Mac. Millan, New York)

Dutta A.C. (2000) A Classbook of Botany

(Oxford University Press, UK)

Ganguli, Das Dutta (2011) – College Botany Vol

I, II and III (New Central Book Agency, Kolkata)


Publications

Microbiology–6th Edition (2006), Pelczar M.J.,

Chan E.C.S., Krieg N.R., The McGraw Hill

Companies Inc. NY

Prescott‘s Microbiology, 8th edition (2010),

Joanne M Willey, Joanne Willey, Linda

Sherwood, Linda M Sherwood, Christopher J

Woolverton, Chris Woolverton, McGrawHil

Science Enginering, USA


Unit I Immunology

1.1 Innate and acquired immunity, Organs of

immune system :

1.2 First, second and third line of defense.

Mechanism of innate immune response.

1.3 Natural - Active and passive immunity.

1.4 Artificial immunity - Active and passive

immunity.

1.5 Organs of immune system – primary lymphoid

organs, secondary lymphoid organs.

15

Lectures

Unit II Microbial genetics

2.1 Genetic analysis in Bacteria- Prototrophs,

Auxotrophs.

2.2 Bacteriophages: Lytic and Lysogenic

Development of Phage.

2.3 Mechanism of Genetic Exchange in Bacteria:

Conjugation; Transformation; Transduction;

(Generalized Transduction, Specialized

Transduction)

15

Lectures

Unit III Population genetics

3.1 Genetic Structure of Populations – Genotypic

Frequencies and Allelic Frequencies, Hardy-

Weinberg Law and its assumptions.

3.2 Genetic Variations in Populations- Measuring

Genetic Variation at Protein Level and

measuring Genetic

3.3 Variations at DNA level, Natural Selection.

Genetic Drift. Speciation .

3.4 Role of Population Genetics in Consevation

Biology

15

Lectures

Ref:

Genetics, (2006) Strickberger MW - (Prentice

Hall, India)

Human Genetics- A. M. Winchester – MacMillan

Press

Microbial Genetics- Freifelder –Narosa

Publishing House

Kuby immunology, Judy Owen , Jenni Punt ,

Sharon Stranford., 7th edition (2012), Freeman

and Co., NY

Textbook of basic and clinical immunology, 1st

edition (2013), Sudha Gangal and Shubhangi

Sontakke, University Press, India

Immunology, 7th edition (2006), David Male,

Jonathan Brostoff, David Roth, Ivan Roitt,

Mosby, USA.

Introduction to Immunology- C V Rao- Narosa

Publishing House


Unit I Model organisms in biology

1.1 Define model organisms, Concept of Animal

models, Types of animal models.

1.2 Criteria for selection of model system

1.3 Prokaryotic: E. coli and its maintenance

15

Lectures

1.4 Eukaryotic: i. Fungi: Saccharomyces

cerevisiae, and Neurospora crassa

1.5 Plantae: Zea mays and Pisum sativum

1.6 Animals: Albino mice, Guinea pigs, Hamsters,

Monkey, etc, and their maintenance.

1.7 Drosophila and its maintenance

Unit II Prokaryotic and Eukaryotic Replication

2.1 DNA Replication in Prokaryotes and

Eukaryotes- Semi-conservative DNA replication,

2.2 DNA Polymerases and its role,

2.3 E.coli Chromosome Replication,

2.4 Bidirectional Replication of Circular DNA

molecules. Rolling Circle Replication,

2.5 DNA Replication in Eukaryotes

2.6 DNA Recombination – Holliday Model for

Recombination Transformation

Unit III DNA mutations and DNA repair

3.1 Definition and Types of Mutations.

3.2 Mutagenesis and Mutagens.( Examples of

Physical, Chemical and Biological Mutagens)

Types of Point Mutations,

3.3 DNA repair - Photoreversal, Base Excision

Repair, Nucleotide Excision Repair, Mismatch

Repair, SOS Repair and Recombination Repair.

15

Lectures

Ref:

Genes XI, 11th edition (2012), Benjamin Lewin,

Publisher - Jones and Barlett Inc. USA

Molecular Biology of the Gene, 6th Edition

(2008), James D. Watson, Pearson Education,

Inc. and Dorling Kindersley Publishing, Inc. USA

Molecular Biology, 5th Edition (2011), Weaver

R., McGraw Hill Science. USA

Fundamentals of Molecular Biology, (2009), Pal

J.K. and Saroj Ghaskadbi, Oxford University

Press.

Molecular Biology: genes to proteins, 4th edition

(2011), Burton E Tropp Jones& Bartlett

Learning, USA

iGenetics A Molecular Approach 3rd Edition

Peter J. Russell.

Molecular Biotechnology-Principles and

Applications of Recombinant DNA Technology

3rd Edition Glick B.R., Pasternak J.J., Patten C.L.

PRACTICALS

Semester II

BUSBTP201: Basic Chemistry (Credits: 02)

1. To determine enthalpy of dissolution of salt like KNO3

2. Determine the rate constant for hydrolysis of ester using HCl as a catalyst

3. Study the kinetics of reaction between Thiosulphate ion and HCl

4. Study reaction between potassium Persulphate and Potassium Iodide kinetically and

hence to determine order of reaction

5. Determination of amount of K oxalate and oxalic acid in the given solution

Titrimetrcially

6. Determination of percent composition of BaSO4 and NH4Cl in the given mixture

Gravimetrically.

7. Qualitative detection of amino acids and proteins.

8. Colorimetric estimation of proteins by Biuret method.

9. Estimation of Vitamin C by Iodometric titration.

10. Paper chromatography of amino acids and sugars.

11. Thin layer chromatography of lipids.

BUSBTP202: Life Sciences (Credits: 02)

1. Permanent slides of - Nostoc, Anabaena, Oscillatoria, Rivularia.

2. Study of Mammalian Blood-

A. Blood count (WBC and RBC) using Haemocytometer

B. Polychrome staining

3. Study of Human Blood Groups

4. Study of Mammalian Kidney and Heart (Permanent slides)

5. Soil studies (Physical characteristics of soil-acidity, alkalinity, pH).

6. To estimate Dissolved oxygen in a given water sample

7. Study of Air Flora and colony character.

BUSBTP203: Biotechnology (Credits: 02)

1. Effect of temperature, pH and osmotic pressure on cell

2. Study of Drosophila.

3. Enumeration of viable microorganisms by

a. Serial Dilution,

b. Pour Plate,

c. Spread Plate Method

4.Enumeration by

a. Breed‘s count,

b. Brown's Opacity tubes

5.Industrial Visit and report.

6. Qualitative assay of enzymes (Amylase, Urease, Dehydrogenase, catalase

7. Enzyme Kinetics: effect of pH, temperature, Subtract concentration , Inhibitors – on

enzyme.

Semester – I and II

Ability Enhancement Course 1 (FC I)

Ability Enhancement Course 2 (FC II)

Course Code Title Credits

USBT 107 Societal Awareness 02

Unit I

Overview of Indian Society 1.1 Understand the multi-cultural diversity of Indian

society through its demographic composition:

1.2 Population distribution according to religion, caste,

and gender;

1.3 Appreciate the concept of linguistic diversity in

relation to the Indian situation;

1.4 Understand regional variations according to rural,

urban and tribal characteristics;

1.5 Understanding the concept of diversity as difference

15

Lectures

Unit II

Concept of Disparity- I 2.1 Understand the concept of disparity as arising out of

stratification and inequality;

2.2 Explore the disparities arising out of gender with

special reference to violence against women, female

foeticide (declining sex ratio), and portrayal of

women in media;

2.3 Appreciate the inequalities faced by people with

disabilities and understand the issues of people with

physical and mental disabilities

2.4 Concept of Disparity-II Examine inequalities

manifested due to the caste system and inter-group

conflicts arising thereof;

2.5 Understand inter-group conflicts arising out of

communalism;

2.6 Examine the causes and effects of conflicts arising out

of regionalism and linguistic differences

15

Lectures

Unit III

The Indian Constitution 3.1 Philosophy of the Constitution as set out in the

Preamble;

3.2 The structure of the Constitution-the Preamble, Main

Body and Schedules;

3.3 Fundamental Duties of the Indian Citizen; tolerance,

peace and communal harmony as crucial values in

strengthening the social fabric of Indian society;

3.4 Basic features of the Constitution

3.5 Significant Aspects of Political Processes he party

system in Indian politics;

3.6 Local self-government in urban and rural areas; the

73rd and 74th Amendments and their implications

for inclusive politics;

3.7 Role and significance of women in politics

15

Lectures

Topics for Project Guidance: Growing Social Problems in India: 1. Substance abuse‐ impact on youth & challenges for the future

2. HIV/AIDS‐ awareness, prevention, treatment and services

3. Problems of the elderly‐ causes, implications and response

4. Issue of child labour‐ magnitude, causes, effects and response

5. Child abuse‐ effects and ways to prevent

6. Trafficking of women‐ causes, effects and response

Course Code Title Credits

USBT 207 Globalization, Ecology and Sustainable Development

02

Unit I

Globalisation and Indian Society

1.1 Understanding the concepts of liberalization,

privatization and globalization;

1.2 Growth of information technology and

communication and its impact manifested in everyday

life;

1.3 Impact of globalization on industry: changes in

employment and increasing migration;

1.4 Changes in agrarian sector due to globalization; rise

in corporate farming and increase in farmers’

suicides.

1.5 Human Rights Concept of Human Rights; origin and

evolution of the concept;

1.6 The Universal Declaration of Human Rights;Human

Rights constituents with special reference to

Fundamental Rights stated in the Constitution

15

Lectures

Unit II

Ecologyand Sustainbale Development 2.1 Importance of Environment Studies in the current

developmental context;

2.2 Understanding concepts of Environment, Ecology

and their interconnectedness;

2.3 Environment as natural capital and connection to

quality of human life;

2.4 Environmental Degradation causes and impact on

human life; Sustainable development, concept and

components; poverty and environment

15

Lectures

Unit III

Understanding Stress and Conflict 3.1 Causes of stress and conflict in individuals and

society;

3.2 Agents of socialization and the role played by them in

developing the individual;

3.3 Significance of values, ethics and prejudices in

developing the individual;

3.4 Stereotyping and prejudice as significant factors in

15

Lectures

causing conflicts in society.

3.5 Aggression and violence as the public expression of

conflict

3.6 Managing Stress and Conflict in Society Types of

conflicts and use of coping mechanisms for

managing individual stress;

3.7 Maslow’s theory of self‐actualisation;Different

methods of responding to conflicts in society;

3.8 Conflict‐resolution and efforts towards building peace

and harmony in society

Topics for Project Guidance: Growing Social Problems in India:

1. Increasing urbanization, problems of housing, health and sanitation;

2. Changing lifestyles and impact on culture.

3. Farmers’ suicides and agrarian distress.

4. Debate regarding Genetically Modified Crops.

5. Development projects and Human Rights violations.

6. Increasing crime/suicides among youth.

f.y.b.sc....mordant and fixative. 3.5 natural and synthetic dyes. 3.6 simple staining, differential...

Documents