functionalized magnetic beads for bioassay

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AMO-MagTM

Functionalized Magnetic Beads For Bioassay

2

AMO-MAGTM

Advanced Material On nano-Fibers0 1 K e y F e a t u r e s

AMO-MagTM beads have core-shell structure by superparamagnetic nanoparticles

and silica with uniform size.

Magnetic core

Silica shell

Target Antigen

Target Protein

Target Gene

http://pubs.rsc.org/en/content/articlehtml/2013/nr/c3nr04777f


3

0 1 K e y F e a t u r e s

High Uniformity Rapid Response High Water Dispersion

Lager Specific Area Best efficiency Low iron exposure

Magnetic core

Silica shell

Target Antigen

Target Protein

Target Gene

AMO-MAGTM

Advanced Material On nano-Fibers





4

0 1 K e y F e a t u r e s

High Uniformity Rapid Response High Water Dispersion

Lager Specific Area Best efficiency Low iron exposure

AMO-MAGTM


Magnetic core

Silica shell

Target Antigen

Target Protein

Target Gene





0 2 H i g h U n i f o r m i t y AMO-MAGTM


High Uniformity

AMO-MagTM beads have a uniform size.

0 2 R a p i d R e s p o n s e AMO-MAGTM


Rapid Response

AMO-MagTM beads have high magnetic content and fast response.

Magnetic Separation Time(In dH2O)

AMO-Mag (300nm) Competitor I (1 um) Compatitor II (1.6 um)

Magnetic beads concentration : 1 mg/ml

0 2 H i g h W a t e r D i s p e r s i o n AMO-MAGTM


High Water Dispersion

AMO-MagTM beads have high water dispersibility.

0 2 L a g e r S p e c i f i c A r e a AMO-MAGTM


AMO-MagTM beads have lager specific area.

Lager Specific Area

AMO-Mag magnetic beads (300nm) Other magnetic beads (1㎛)

18.8

102.8

1.8

0

1

2

3

4

0

4

8

12

16

20

AMO-Mag Compatitor

g/c

m3

m2/g

Surface area Density of bead

Surface area & density of bead

AMO-Mag@Ab

Capture mAb

C-reactive proteinDetect pAb

0 2 B e s t e f f i c i e n c y AMO-MAGTM


Best efficiency

The highly defined process of AMO-MagTM will ultimately offer the best efficiency

for specific binding and reproducibility for precise analysis.

0 500 1000 1500 2000

0

5000

10000

15000

20000

25000

30000

Flu

ore

sc

en

ce

In

ten

sit

y(a

.u.)

AFP[ng/ml]

AMO-Mag@(T)StAv(300nm)

Market leader I(1m)

0.01 0.1 1 10 100

-0.5

0.0

0.5

1.0

1.5

2.0

2.5

3.0

3.5

A450

CRP[ng/ml]


Market leader I(1m)

Market leader II(1.6m)

CRP(Sandwich assay) AFP(Sandwich assay) Progesterone(Competition assay)

AMO-Mag (300nm)

Competitor I (1m)

Competitor II (1.6mm)

AMO-Mag (300nm)

Competitor I (1m)

0 10 20 30 40

0

2500

5000

7500

10000

12500

15000

Flu

ore

scen

ce In

ten

sit

y(a

.u.)

Progesterone[ng/ml]


Market leader I(1m)

Competitor I (1m)

AMO-Mag (300nm)



Best efficiency



Immunoassay test



Best efficiency



Red Blood Cell separation

AMO-MagTM (300 nm) Competitor I (1 m) Competitor II (1.6 m)

RBC

RBC antibody conjugated Magnetic beads

• Time : 5 sec• Efficiency : 99.9%

• Time : 15 sec• Efficiency : 93%

• Time : 10 sec• Efficiency : 78%



Best efficiency



Target enrichment for Next Generation Sequencing

61.2 66.4 62.3 65.462.2 64.2

0

20

40

60

80

AMO-Mag

Average Amount after Post PCR(ng)

96.3 95.9 96.1 96.1 96.5 96.4

0

50

100

AMO-Mag

Filtered Ratio(%)

Uniformity

0.40 0.40 0.40 0.39 0.39 0.40

0

0.5

1

AMO-MagAMO-MagTM Competitor

0.2x Mean Depth Coverage Uniformity(%)

94.1 94.0 93.5 93.4 94.0 93.7

0

50

100

AMO-Mag

Duplication Ratio(%)

3.32 2.96 2.96 3.28 3.44 3.10 0

50

100

AMO-Mag

On Target Base Ratio(%)

39.3 38.9 38.8 40.8 39.2 39.7

0

50

100

AMO-Mag

0.5x Mean Depth Coverage Uniformity(%)

82.1 82.3 82.8 82.8 82.6 82.2

0

50

100

AMO-Mag

1x Mean Depth Coverage Uniformity(%)

52.4 51.8 53.2 52.4 52.8 52.3

0

50

100

AMO-Mag

AMO-MagTM AMO-MagTM Competitor

AMO-MagTM Competitor





Competitor

AMO-MagTM beads can be applied to various applications by supplying various

surface functional groups.

AMO-MAGTM

Advanced Material On nano-Fibers0 3 A M O - M a g T M

Tosyl

Carboxyl (COOH)

NHS

Amine (NH2)

Hydroxyl(OH)

Streptavidin

Protein A, G

Cell separation

Strip sensor

Nucleic acid extraction

Immunoassay Protein purification

Next generation sequencing

http://www.miltenyibiotec.com/en/research-areas/stem-cell-research/satellite-cells/cell-separation.aspx

AMO-ARA kit

DNA/RNA extraction kit

AMO-ARATM delivers high yield of viral RNA in all samples tested. Added the viral

sup to plasma, serum, urine and rinse liquid from swabs and extracted the viral RNA

from each sample using AMO-ARA . The extracted viral RNA was used the qRT-PCR

and agarose gel electrophoresis.

AMO-MAGTM

Advanced Material On nano-Fibers0 1 AMO-ARATM Viral RNA extraction Kit

Isolation of viral RNA from human plasma, serum, urine and liquid from swabs.

AMO-MAGTM

Advanced Material On nano-Fibers0 2 AMO-ARATM MAG-NA Kit (Blood DNA isolation)

AMO-ARATM Human Blood genomic DNA extraction kit offer the high efficiency and fast reaction to extract pure DNA from Human blood for precise analysis.

53.1849.42

35.9932.65

37.2

0

20

40

60

AMO-ARA Competitor A Competitor B Competitor C Competitor D

DN

A C

on

cen

trat

ion

per

0.2

mL

of

Blo

od

[ng/

L]

1.82 1.81 1.78 1.72 1.71

2.15

1.681.87 1.79

0.72

0

1

2

3

AMO-ARA Competitor A Competitor B Competitor C Competitor D

DN

A p

uri

ty

260/280 260/230

Human blood DNA Extraction Data

AMO-MAGTM

Advanced Material On nano-Fibers0 3 AMO-ARATM cfDNA extraction Kit

AMO-ARATM cfDNA isolation kit offer the best efficiency and purity for precise analysis.

97.7

69.275.9

40.3

58.9

39.2

0

20

40

60

80

100

120

AMO-ARA CompetitorA

CompetitorB

CompetitorC

CompetitorD

CompetitorE

cfD

NA

Co

nce

ntr

atio

n

per

1m

L o

f p

lasm

a[p

g/

L]

DNA Purification from Plasma

Extracts DNA from 1mL of plasma with a simple protocol

Thank youWorld First! World Best!

functionalized magnetic beads for bioassay

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