functional genomics research stream qpcr data lab...

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Functional Genomics Research Stream Research Meeting: November 30 2010 Closing Ceremonies Final Reports Post on Results Central - Important When: Sunday, December 5 @ 10PM Professionalism, quality ... General introduction ... Conclusions, reflections ... Clear section titles (bold) ... Name, Date, Research Stream ... qPCR Data New data is available ... Expect it to be fully analyzed in report ... Check Results Central Lab Cleaning Post on Results Central Freezer Boxes ... Tubes in Racks in -20C ... Plates in 4C ... Incubator ... Pipette Calibration ... General lab cleaning ...

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Page 1: Functional Genomics Research Stream qPCR Data Lab Cleaningfg.cns.utexas.edu/fg/course_materials_-_fall_2010_files/2010-11-30.pdf · 30/11/2010  · •The Elevator Pitch - Be Prepared

Functional Genomics Research StreamResearch Meeting: November 30 2010

Closing Ceremonies

Final Reports

• Post on Results Central - Important

• When: Sunday, December 5 @ 10PM

• Professionalism, quality ...

• General introduction ...

• Conclusions, reflections ...

• Clear section titles (bold) ...

• Name, Date, Research Stream ...

qPCR Data

• New data is available ...

• Expect it to be fully analyzed in report ...

• Check Results Central

Lab Cleaning

• Post on Results Central

• Freezer Boxes ...

• Tubes in Racks in -20C ...

• Plates in 4C ...

• Incubator ...

• Pipette Calibration ...

• General lab cleaning ...

Page 2: Functional Genomics Research Stream qPCR Data Lab Cleaningfg.cns.utexas.edu/fg/course_materials_-_fall_2010_files/2010-11-30.pdf · 30/11/2010  · •The Elevator Pitch - Be Prepared

Internships, NSC

• Post(s) on Results Central

• Remember to register ...

• Consider your options moving forward ...

Evaluations

• eCIS email should have been sent ...

• Please participate ...

Take Home Messages

The Details Matter

• Independent of your long-term plans

• Reagents, pH, buffers ...

• Pipette technique ...

• Aseptic technique ...

• Sensitive reactions ...

• How a protocol or kit works ...

Page 3: Functional Genomics Research Stream qPCR Data Lab Cleaningfg.cns.utexas.edu/fg/course_materials_-_fall_2010_files/2010-11-30.pdf · 30/11/2010  · •The Elevator Pitch - Be Prepared

Consistency Matters

• Scientific procedures are a careful dance ...

• Timing, conditions, verified reagents matter ...

• Diagnostics are your friend ...

• Assumptions will bite you in the ...

• Control as much as possible ...

• Trust few peers ...

The Process Matters

• Scientific procedures are a careful dance ...

• The dance is wicked ugly at first ...

• Make a mess.

• Evaluate.

• Make less of a mess.

• Repeat.

Communication Matters

• Laboratory Issues: Reagents, concentrations, protocols, procedures.

• Scientific Issues: Papers, techniques, ideas.

• Thinking and communicating on your feet.

• The Elevator Pitch - Be Prepared

• Be sensitive to who your audience is ...

• Could you convince your mother?

Things Change

• Our basic understanding changes.

• Questions change.

• Social interest changes.

• Technology changes.

• People come, people go.

• Be prepared for change, predict it.

Page 4: Functional Genomics Research Stream qPCR Data Lab Cleaningfg.cns.utexas.edu/fg/course_materials_-_fall_2010_files/2010-11-30.pdf · 30/11/2010  · •The Elevator Pitch - Be Prepared

Life Comes in Seasons• Current Season: Perspective & Tool-belt

• Understand as much as possible; commitment not needed.

• Future Seasons:Vector, Focus, Achieve & Expertise

• You are whomever you want to be.

• Appreciate seasons for what they allow and bring no matter how limiting they may feel.

Leadership Matters

• Positions rarely given ...

• Opportunities must be sensed ...

• Reward often delayed ...

• Personality dependent ...

People Matter

• Remember when I said “Trust Few”?That was peers.

• Your future managers, advisors, bosses, etc are a major key to your growth, development and success.

• Select them carefully.

• People first, science second.

Page 5: Functional Genomics Research Stream qPCR Data Lab Cleaningfg.cns.utexas.edu/fg/course_materials_-_fall_2010_files/2010-11-30.pdf · 30/11/2010  · •The Elevator Pitch - Be Prepared

Your Life Matters

• Try to keep track of what you enjoy, what you do not.

• Make choices to enrich for the former.

• Don’t be passive.

• Make sacrifices, be patient.

• Be sensitives to seasons where choice is wide open to you. Don’t pretend it’s not.

• Enjoy other things. Do other things.

Questions?