fraunhofer institute - biomedical engineering ibmt · © fraunhofer fraunhofer-gesellschaft, the...

© Fraunhofer IBMT

Fraunhofer Institute - Biomedical Engineering IBMT

Brokerage Event 2016 – Regenerative Medicine and Bioinformatics

Ostrava, Czech Republic, May 19th, 2018

18.04.2016

© Fraunhofer

Researcher

Discovery of the “Fraunhofer

lines” in the solar spectrum

Inventor

New methods for processing

lenses

Entrepreneur

Director and partner in a

glassworks

Joseph von Fraunhofer (1787 – 1826)

© Fraunhofer-Gesellschaft

“Fraunhofer lines”

© Deutsches Museum

18.04.2016

© Fraunhofer

Fraunhofer-Gesellschaft, the largest organization for

applied research in Europe

67 institutes and research units

24,000 staff

more than €2.1 billion annual research budget totaling. Of this sum, around

1.8 billion euros is generated through contract research

roughly two thirds of this sum is generated through contract research on

behalf of industry and publicly funded research projects

roughly one third is contributed by the German federal and Länder

governments in the form of base funding

“Fraunhofer lines”

18.04.2016

© Fraunhofer

The Fraunhofer-Gesellschaft at a Glance

The Fraunhofer-Gesellschaft undertakes applied research of direct utility

to private and public enterprise and of wide benefit to society.

24,000 staff

More than 70%

is derived from

contracts with industry

and from publicly

financed research

projects.

Almost 30%

is contributed by the

German federal and

Länder Governments.

67 institutes and research units Fin

ance v

olu

me

€2.1 billion

2015

Co

ntr

act

Researc

h

€1.8

billion

Major infrastructure

capital expenditure and

defense research

18.04.2016

© Fraunhofer

Joseph von

Fraunhofer

Director and partner in

a glassworks

Discovery of the

“Fraunhofer lines” in

the solar spectrum

New methods for

processing lenses

Research volume:

approx. € 2 billion

annually

Research and

development on behalf

of industry and state

mp3 music format,

white LED, high-

resolution thermal

camera

Researcher

Inventor

Entrepreneur

The Fraunhofer-

Gesellschaft

18.04.2016

http://www.fraunhofer.de/fhg/Images/jvf_brosch_tcm5-5806.pdf

© Fraunhofer

The Fraunhofer-Gesellschaft

Locations in Germany

67 institutes and research units

24,000 staff

18.04.2016

© Fraunhofer

Fraunhofer worldwide

Dubai

Bangalore

Jakarta

Beijing Seoul

Cairo

Santiago de Chile

Singapore

Brussels

Porto

Vienna

Bolzano Graz

Wrocław

Gothenburg

Salvador

Paris

São Paulo

Campinas

Subsidiary

Center

Project Center

ICON / Strategic Cooperation

Representative / Marketing Office

Senior Advisor

Stellenbosch

Boston

Plymouth

East

Lansing San José

Newark Storrs

Glasgow

Southampton Dublin

Budapest

Tokyo

Sendai

Osaka

Pretoria

Jerusalem College Park

Vancouver

Londo

n

Hamilton

Zur Lavon

Kuala Lumpur

18.04.2016

© Fraunhofer

Fraunhofer fields of research

Health and Environment

Communication and Information

Production and Services

Mobility and Transportation

Energy and Resources

Safety and Security

18.04.2016

© Fraunhofer

Microelectronics

EMFT, ENAS, FHR, HHI, IAF, IIS, IISB, IMS, IPMS, ISIT, IZM

Associated members : ESK, FOKUS, IDMT, IKTS, IZFP

Fraunhofer Groups

ICT AISEC, ESK, FIT, FKIE, FOKUS, IAIS, IAO, IDMT, IESE, IGD,

IOSB, ISST, ITWM, IVI, MEVIS, SCAI, SIT

Associated members: HHI, IIS

Life Sciences EMB, IBMT, IGB, IME, ITEM, IVV, IZI

Light & Surfaces

FEP, ILT, IOF, IPM, IST, IWS

Production IFF, IML, IPA, IPK, IPT, IWU, UMSICHT

Materials and Components –

MATERIALS

EMI, IAP, IBP, ICT, IFAM, IKTS, ISC, ISE, ISI, IWES, IWM, IZFP,

LBF, WKI

Associated members: IGB, IIS, ITWM Institutes outside groups: MOEZ, PYCO, IRB,

Defense and Security VVS EMI, FHR, FKIE, IAF, ICT, INT, IOSB

Associated members: HHI , IIS, ISI

16.10.2015

18.04.2016

© Fraunhofer

1

2

3

5

6

4

Biomedical Engineering IBMT

Interfacial Engineering and Biotechnology IGB

Molecular Biology and Applied Ecology IME

Toxicology and Experimental Medicine ITEM

Process Engineering and Packaging IVV

Cell Therapy and Immunology IZI

Fraunhofer Group for Life Sciences

Stuttgart

Leipzig

Aachen

Freising

Hannover

Sulzbach

St. Ingbert

Potsdam

Schmallenberg

1

2

5

3

3

4

6

6

18.04.2016

© Fraunhofer

Key data

Foundation in 2000

Central office: Hannover

7 member institutes

Scientific staff1: 684

Percentage of industrial revenues2: 31%

Budget3: €168 million

Chairman: Prof. Dr. Rainer Fischer

Head of Central Office: Dr. Claus-Dieter Kroggel

Business areas

Medical Translational Research and Biomedical Technology

Regenerative Medicine

Healthy Foods

The New Potential of Biotechnology

Process, Chemical, and Herbicide Safety

Fraunhofer Group for Life Sciences

1 December 31, 2014 2 2014 3 budget 2015

18.04.2016

© Fraunhofer IBMT

Fraunhofer IBMT - Locations

Site

St. Ingbert

Ensheimer Strasse 48

66386 St. Ingbert

Germany

Headquarters

Sulzbach

Joseph-von-

Fraunhofer-Weg 1

66280 Sulzbach

Germany

Branch

Münster

Mendelstrasse 11

48149 Münster

Germany

Branch

Cambridge

Babraham Research

Campus

Meditrina Building

CB22 3AT Cambridge

United Kingdom

Branch

Coquimbo

Universidad Católica

del Norte

Larrondo 1281

Coquimbo

Chile

Branch

Berlin

Im Fraunhofer-

Forum Berlin

Anna-Louisa-

Karsch-Strasse 2

10178 Berlin

Germany

18.04.2016

© Fraunhofer IBMT

Fraunhofer Institute for Biomedical Engineering IBMT Cryo & Stem Cell Technology

Pluripotency & Regeneration

Biomedical Optics

Automation Processes

Marine Biopolymers (Coquimbo/Chile)

Cooperation Laboratory EFPIA (Babraham/UK)

Bioprocessing & Bioanalytics Biomonitoring & Cryobanking

Cellular Bioprocessing

Preclinical Nanotechnology & Nanotoxicology

Medical Ultrasound Ultrasound Systems/Clinical Applications

Biomedical Ultrasound Research

High-Frequency Piezosystems

Technical Ultrasound Technical Ultrasound Systems

Transducer Development

Manufacturing Technology (ISO 9001 & 13485)

Simulation

Biomedical Microsystems Microsensors & Microfluidics

Biotelemetry

Active Implants

Medical Engineering & Neuroprosthetics Neuromonitoring

Neuroprosthetics

Silicone Technology

Health Information Systems

MEDICAL

BIOTECHNOLOGY

ULTRASOUND

BIOMEDICAL

ENGINEERING

Main

Departments 18.04.2016

© Fraunhofer IBMT

Fraunhofer IBMT – Data and Facts (2015)

3 main departments

6 departments

140 staff

16,5 Mio € IBMT - total budget

foundation member of Fraunhofer Group Life Sciences

Fraunhofer Bioarchive

Gemeinschaft Deutscher Kryobanken (GDK)

18.04.2016

© Fraunhofer IBMT

Cryo & Stem Cell Technology


Biomedical Optics


Marine Biopolymers (Coquimbo/Chile)

Cooperation Laboratory EFPIA (Babraham/UK)

Bioprocessing & Bioanalytics

Biomonitoring & Cryobanking

Cellular Bioprocessing

Preclinical Nanotechnology & Nanotoxicology

Medical Biotechnology

18.04.2016

© Fraunhofer IBMT

Cryo & Stem Cell Technology


Automated pipelines for hiPSC work (reprogramming, passaging, differentiation, QC)

Ultra-high viscosity alginate substrate-engineering for regenerative biomedicine

Biomedical Imaging

Cryo-, ultra-high pressure-, high-sensitivity and Raman-scanning-microscopy


Automated cryobanking, advanced cryopreservation processes and media

Parallelization of microfluidic-based robotic platforms

Marine biopolymers

Production of ultra-high viscosity alginate for biomedical research

Main Department Medical Biotechnology

18.04.2016

© Fraunhofer IBMT


hiPSC Work Automation

Bioreactors for the expansion of hiPSCs on alginate microcarriers

Robotic arm for fully automated culture of hiPSCs in standard flasks

Microfluidic platform for hiPSC differentiation in hanging drops

Evaluation of pluripotency in human cells:

qPCR and in situ hybridization techniques

flow cytometer and immunocytochemistry

automated time-lapse imaging systems

Cryopreservation

Development of protocols & media for sterile vitrification of hiPSCs and hiPSC

derivatives

Cryogenic storage systems with -100°C workbench for automated cryobanking

Pluripotency and Regeneration

Pluripotency and adulthood: the planarian model for pluripotency-based regeneration

Ultra-high viscosity alginate substrate-engineering for hiPSC-derived organoids


18.04.2016

© Fraunhofer IBMT


Automated biobanking of biological material, micro organisms and

clinical samples under BSL 2

Induced pluripotent stem cells (iPSCs)

DIN EN ISO9001:2015 conform

Optimization and validation of automated cell culture processes

Cultivation of cell lines and induced pluripotent stem cells

Production von HIV-1 pseudo-type viruses

(“Good Clinical Laboratory Practice” (GCLP) - conform)

Beat-coupled cell culture in bioreactors

Reprogramming und differentiation of induced pluripotent stem cells

Automation of cell-based assays and processes

Neutralization assays, ELISpot, aliquoting, embryonic stem cell assay

Optimization and validation

QM-conform


18.04.2016

© Fraunhofer IBMT

Mobile Laboratories according to Biosafety Level

(BSL)-2 and -3

Fraunhofer IBMT (von Briesen)

Mobile Laboratory BSL-3 Trailer Solution

Diesel tank Cooling kit

USV and

Storage compartment 24 Volt supply

Hydraulic controls

Autoclave

water supply system

Main entrance

Foil affixed to the truck

showing the interior


MOBILE DIAGNOSTIC LABORATORY BIOSAFETY

LEVEL 3 (BSL-3)

•Equipped with diagnostic and cryostorage facilities.

•In conformance with German standards for mobile laboratory units and the

Infection Protection Act (IfSG).

•Licensed 2009 for field and public road operation.

•Independent from electricity, water or sewage connections.

•65 kW self-sufficient power supply with its 1,000 liter diesel fuel tank

•Hydraulic adjustments on the vehicle are geared to level off the vehicle in

any terrain

•The operation from a central location of the vehicle via a user friendly

interface of the vehicle control system.


MOBILE DIAGNOSTIC LABORATORY BIOSAFETY

LEVEL 3 (BSL-3)

•Medically equipped admission and treatment unit

•Gradual negative pressure between rooms

•Setup of the systems is designed for easy change of devices

•Two class II biological safety cabinets

•Pass-through autoclave equipped with vacuum steam sterilization

•Mobile cryobank through its 300 l nitrogen tank aboard

•Remote control and support of the semi-trailer via Internet


Medically equipped admission and treatment unit

Touch panel for

technical control


Laboratory I

Gimbal-mounted

incubator


Laboratory II

Mobile Laboratory – Arriving Cape Town


Township in Western Cape Province


Operation near Caledon Township


Technical control

Remote support

Fraunhofer IBMT

3rd Generation:

The project „Labor der Zukunft“ is funded by Saarland‘s Ministry for Economic Affairs and Science.

© Fraunhofer IBMT

Example: EBiSC-IMI-Project, Biobank Location Sulzbach

European Bank for induced pluripotent Stem Cells

IBMT project group in Cambridge

Storage and provision of well-characterized, disease-relevant

iPS cells from well-selected patient cohorts

Development of a „gold standard“ in the area of iPS cultivation,

cryopreservation and characterization

Development of automation strategies for the expansion and the

storage of iPS cells

Building-up logistical structures and sales

18.04.2016

© Fraunhofer IBMT

Automation Technologies for iPSC Processes

Cell Expansion Cell Identification

High-throughput Screenings

Cellbanking

Joint development with TECAN.

„Hanging-Droplet“ culture approach for automated

addition of factors with the aid of a pipette roboter.

First fully automated plant of ASKION for liquid nitrogen cooling.

Placing samples into the Dewar vessel programming destination into the computer automatic storage and removal of the samples.

Solution for future biobanks all over the world. 18.04.2016

© Fraunhofer IBMT

Qualified Biobanking: Market Entry with Fully Automated Cryo Plants

After 12 years time: commercially available biobank technology platform (ASKION – HAMILTON)

System provider for therapeutically and diagnostically-oriented biobanks

Development of new cryomedia, optimized freezing protocols, contract storage, planning and validation of industrially and clinically-structured highly-modern biobanks

CRYO-BREHM – German Cellbank for Wildlife

Contribution in large European project » EBiSC«: Construction of an iPS cellbank

„C-line®system“ - ASKION- GmbH (Gera)

18.04.2016

© Fraunhofer IBMT

Automation Technologies for iPSC Processes:

Fully automated Cell Banking

Solution for future biobanks all over the world. 18.04.2016

© Fraunhofer IBMT

Fraunhofer Institute - Biomedical Engineering IBMT

Brokerage Event 2016 – Regenerative Medicine and Bioinformatics

Ostrava, Czech Republic, May 19th, 2018

18.04.2016

Thank you for your attention

fraunhofer institute - biomedical engineering ibmt · © fraunhofer fraunhofer-gesellschaft, the...

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