formula thailand.pdf

8/19/2019 formula thailand.pdf

1/47

Development of Xanthone

Powder Production Technology

Wilaisri Limphapayom

Post harvest and Products Processing

Research and Development Division

Department of Agriculture.


2/47

Mangosteen Fruit

Garcinia mangostana Linn.

is tropical fruit available in Thailand.

Nutraceutical mangosteen

composition

is mangosteen fruit pulp and rind.


3/47

Extracts from Mangosteen Rind

The extracts from mangosteen rind mainly

compose of xanthone , anthocyanins and

tannins.

Xanthone group in mangosteen extractscompose of alpha-mangostin (1,3,6-

trihydroxy-7-methoxy-2,8-bis-(3-methyl-

but-2-enyl)-xanthen-9-one) and gamma-mangostin (1,3,6-trihydroxy-2,8-bis-(3-

methyl-but-2-enyl)-xanthen-9-one). And

others.


4/47

Bioactivities of Xanthone.

The xanthone has demonstrated

antioxidant,

antibacterial,anti-inflammatory activities and

the ability to induce cancer cell.


5/47

Objective

The objective of this research is to

Extraction of xanthone frommangosteen rind by using solvent extraction.

Preparation of mangosteen powder

Utilization of Mangosteen rind extract.


6/47

Material and Method

Extraction of Mangosteen rind.

1. Dried and Powder mangosteen rind.

2. Solvent Extraction by Ethanol, Methanol.

3. Soxlet Extraction./Continuous Extraction.

4. Rotary Evaporator

5. Dried in vacumm oven

6. Accurated weight.


7/47


8/47


9/47

Extraction of Mangosteen rind.

600 gram of dried mangosteen rind are

combined with 3000 L. of ethanol and

continueous extraction for 10 hours.

To receive crude extract by using rotaryevaporator. This method also can be with

methanol and ethyl acetate.


10/47


11/47


12/47

Analysis of Xanthone.

Analysis of Xanthone by HPLC


13/47

Analysis of xanthone by using LC-MS.

Analysis of alpha-mangostin, gamma-mangostin and other xanthone derivatives

have been made by using LC-MS with UV

detector , wavelength 254 nanometer. ,Hypersil ods column, 150x4.6 mm. 5µM,

at 40 C and mobile phase 0.1% (v/v)

acetic acid in water , A and 95% (v/v)methanol ,B . For mobile phase gradient

program, 65-90% B for 40 mins., rate 1 ml

per min., injection volume 10 microlitre.


14/47

Preparation of Mangosteen juice powder.

by using spray dryer.

50 % of mangosteen juice ,

15% of maltodextrin, and

35% water are homogenized before

spraying to receive mangosteen

juice powder.


15/47

Preparation of Xanthone Powder.

Encapsulation of xanthone

by using spray dryer.

20 % of mangostin extract ,

50% of maltodextrin, and

30% mixture of ethanol and water 1:1 are

homogenized before spraying to receive

xanthone powder.


16/47

Result and Discussion.

Ethanol and methanol extracts are 21.75-24.33% and 20.8-22.8% of dried

mangosteen rind respectively.

Mixture of ethyl acetate and water (1:1 v/v)extracts are 7.8-10.36% of driedmangosteen rind.


17/47


18/47

Maceration Mangosteen rind .


19/47

Analysis of xanthone by using LC-MSshowed that one gram of dried

mangosteem rind contained alpha-

mangostin

931.79

microgram

Beta-mangosteen 19.47 microgram and

gartanins 18.7 microgram.


20/47

One gram of methanol extracted

contained

1079.72 microgram , Beta-mangostin 21.10

microgram and gartanins 24.17

microgram.One gram of ethyl acetate extracted

contained alpha-mangostin 782.74

microgram

Beta-mangosteen

19.69

microgram and gartanins 26.04

microgram.


21/47


22/47


23/47


24/47


25/47

Bioactivities toxicity towards cancer cells,MCF7-Breast cancer ,

IC 50 was average 17.35 microgram per ml.

Note. IC50 the amount of extract into 1 ml.solution necessary to decrease by 50%.


26/47

Xanthone powder was manufactured fromethanol and water extract by using Spray

Dryer.

As raw material for food and cosmetics.


27/47

Mangosteen pulb was processed to instantmangosteen powder, mixing with tea andcoffee , cookies and ice-cream.


28/47


29/47


30/47


31/47


32/47


33/47


34/47


35/47


36/47


37/47


38/47

Conclusion

Mangosteen rind extract can be made byusing solvent extraction , such as

Ethanol and methanol by continuous

extraction or maceration.The mangosteen rind extract mainly

compose of alpha-mangostin.


39/47

Conclusion.

Mangosteen pulp juice powder andxanthone powder can be process

by spray drying.For xanthone powder is raw

materials as food in healthy drink

and in cosmetic.


40/47

Conclusion

Xanthone and derivatives have beenshown to have beneficial effects on

some cardiovascular diseases,

including heart disease , hypertension.The protective effect of xanthones in

the cardiovascular system may be

due to their antioxidant,

anti-inflammatory.


41/47

Conclusion

Xanthones may also have

anti-tumor activity

thus potentially havingan influence in the prevention

or treatment ofcertain cancers.


42/47

Conclusion

The actual active ingredients,

Xanthones are mostly in the rind and less

in the fruit.

Nutraceutical for health benefit needimproving general health and wellness in

human.


43/47

Thank You.


44/47


45/47


46/47


47/47

formula thailand.pdf

Documents