(formerly oriental fruit fly investigations0

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Page 1: (Formerly Oriental Fruit Fly Investigations0

UNITED STATES DEPARTMENT OF AGRICULTURE

Agricultural Research Administration

Bureau of Entomology and Plant Quarantine

Division of Fruit Insect Investigations

University of California Hawaii Agricultural Experiment Station

Territorial Board of Agriculture and Forestry

Pineapple Esssarch Institute Hawaiian Sugar Planters* Association

Experiment Ststion°

IUWAU AGfUC. EXP.

DEPT.

INVESTIGATIONS OF FRUIT FLIES IN HAWAII

(Formerly Oriental Fruit Fly Investigations 0

QUARTERLY. HSPOET

January 1 - March 31S 1952 «-

oOo

#

Page 2: (Formerly Oriental Fruit Fly Investigations0

-179-

WORK HKMI3CT X-o-5 - CCUffliDTZX TREATMENTS - J. W« Bslock, Project Lead©?

sasmss

Line Erojiacfe J~g«5«1, l-o-5"2s I-o~5-3, I-O-5-5 and L-o-5-6 INACTIVE-

Forty-four Materials wars screened, as fusiigants against naksd eggs and

larvae of JU djorsalis* Only one EE.-03r5.al, ally! iscthiocyajiate, killed both

eggs and ILarvas lit a low dosage (all larva© and 95 per csnt of tho eggs at4 milligrams per liter)o

Four materials (1s2-3ibroino pentsne / brc;?otrichloro ethane, dibrorao diehlox'oTuBVjhane aid 2"Chlovo allyl alcohol) Icillad 95 per cent of the larvae at lessthan 6 nilligra&s per litor but tfere irKJi^scitive against tho sggs»

Fifty-seven tests vrera cow.plef.ad to atuc^ the rslation of fruit load to

fly noi'tality* Tests wsre conducted 5ji 5 gallon cans and ii-on drusa of

7»7 cu» fb* cap-acsi'-Sgr* Hie fruit loads tested v^re ecmal to ?y4, 1/2 and 3/4.the frea air sjaca in tas fumatorie.* rJalcsd eggs in open fet?.mi dishss t?sre

tested fjiatu3Ltaceously» ■ Thers uas soas evidenca that noa'tiraity t;as depressed

as the froit load ^-sas increased.! She survival from r^aked eggs appaared higher

than that of eggs and larvae in fruit, espsciclly at ths Ictrer dsjuagas, bat

is dus apparently to the delayed lethxl action of EBB uhich snay pssrait hatch

but <»us3 ulticate death to tha young larvae» The usb of nakacl 3[>:g3 is Eaver-

theloss a good rsethod for detarmiaing lov: concentrations of 33DB biologically

sine©, it- appears that small differences :ui ccncantrcvuiort can ba detected mors

precisely this way than trj chesical anc2ysis»

Soixz slight. additilonal evidesco vc-.o obto:lr.sd theb pax^sitisec?, larvae ars

mo;."o resistant to 3DB thrai are uiiTxa^-Ritiac!;! Ir.rvae, but tho results ars very

i3

Ons' Isrgtt losd tost v/as conduc-'isd ;;ith ~S)B in tr.Q 21,0 ok> ft. charibar to

dat^rmiiis t3^3 effect of caclcinj: Eiiitericl en fruit fly rr.ort^lityt Tho total

load «ras :?36l Iba. U-50 lbs. of ini\2DtQci vtxrv^s aud 1S11 lbs. of pineapple)•>'Che fruit was vrfapped in corrugated paper clsevcs and j.acIcGcl in field boxes»

Borage and expos"Jira ucro 3/4 lbs * for 3 hours at 70 ° F« ?rvdt fly mortalityuas compl^ts in the papayas containing an estisutsd fruit fly infestation of

9S992 eggs esad larvae. Thoro was no dotoct".blo injuzc-y to i'JJZvQT? appearance^

03? ice-ipiDg qualities of either tho pinenppleji or papaya?.! -ahics were tested for

toleran«3» Coiabined data to data from /> large load tasts w-th papayas in a

corrugated wrap at -She 3/4 lb»/3 hour dosage show that 20 puparia vjare recowredfrom an ©stircatsd pre~treatment population of 1S7>O77 oggs and larvae, but sines

no adults eiassgsd tiiis might bo interpreted as complete k:13J-9 It i-ioald appear

•chat idaa reccarcendation of such s. treatiasnt could be Bado \dth slrlght risk of

fn2it f3.y susviv;;JL in pineapple which con be classed as a very poor fruit fly

boat with a sscorded infestation of less than 1 larva psr ton of fsf

Page 3: (Formerly Oriental Fruit Fly Investigations0

-180-

The development of new treatments for fruit fliac has had a very pronouncedeffect on the export of frash fruits and vegetables from Hawaii• E:o>orts have

year not quita completed <> Data for ths current year show that SO par cent ofths experts have been fumigated with EDB»

EBB Fumigation of Madf&r 5a Oranges

experiments (97 total to date) ms&sq complsted to determine theeffect of EDB.on Medfly in oranges* The fruit vss cags-infGsted but the resiat-ing infestations were Light* Dosages and exposures used were l/l6 to 3/4 Ibo/1000 cu« ft» Tor 2 hoiirs at 70° P> Tests irare conducted in 5 gallon cans and7o7 cue ft. drums with a viater seal. Fruit loads in the 5 gallon cans wareequal tc approximate^ 3/4 of the free air space, and the fsruitc *&?& ftaaigatad1-ri.thout containers or pacldag material* Tlie fruit load in ths drums was equalto from'30 to 40 per cent of tha chamber voltiffis, and frultc x-?ere fnaagatadnalcad. in burlap sacks, orange boxes, and trood.cn trays» Survivora trarsonly at the lowest dosage tested,. 1/3.6 lbo/1000 cu. >?to

Using tho lasthod of Sincl&rtr c.nct Grar-d?.?.?. rsccr^ry of EDB from an empty

druta of 7«7 an* ft» volume ran froa 4-0 to 57 per coat aftsr 3 hours from an

initial, concentration of 1/2 lb»/l000 cu. ft. The loss is attributed toeorption sines ths drums wars g&s-tight.

lots of fruits and vegetable.-? (144-5 lbs«) ijsre hold to de«3j?.e degrs© of infestation in export co-amociit5.es subject to fruit fly

attacks Collections to date total 423, coaprxca 26,924. lbs» and rapv^sent12 commodities o Of this total papaya and pineapple jinks up 12,172 Ibs» and5?34B lbs., raapsctivsly, appro3ciss?.taly 64 par cent* Inf®station in papayavaries greatly with the stage of n-vtarity, averaging one larva' par 100 Ibso

in -ihe nature green stage and 85 in. the one-half rip© stago* Pinsappls hasess of t&3 lovrest infestation indices among tljs commodities unc?.sr

a-7aragi3ig less than one larva par ton of fruit«

Page 4: (Formerly Oriental Fruit Fly Investigations0

-181-

(IP

^ WORK PROJECT I-o-5« Commodity Treatments - J« VT. Balock, Reject Leader

L±oa projects I-o-S-l, 1-0-5-2, 1-0-5-3* X-o-5-5» and I~o~5-6 INACTIVE DURIKC- QUARTER

Line Ego.jeqft 1-0-5^4.. Tg.fgs'k ,®&\l^^^^Y^^^^.^F^^^J^WfP^8 £cr-ill Conanodity 'i.'ggatmentso (BalocIcT^HiUEffi&T Ko~3uro&y~~~

■^) .Scrseniag of Materials to_ deterainei their to:d.city to.naked qrrbsand pu^qg of fruit fli93» (Fganlv Hinat?ii)

Forty-fores? inaterials wsr-3 screened as fuaigants against naked eggs and

third-iastar larvae of Da dor3alis« A total of 180 compounds bav© been tasted

to date* The results are shown in table !»

Two uatsrials, brosno chloro nsathana and a!3yl iBotl^iocjnanate, killed all

eggs and laz-VES at the dosages testsdj the methane tjas completely affective at

soraawhsra bstt.3Qn 110 and 200 ngs» par liter, the isothiocyanato at less than

5 ings* par liter<>

Hona of tlia other taaterials killed all of tae eggs but five killed all

larvaeo These five tTora 1,2-dibroiGo pentene, bromo tricliloro etlianej dibromo

diehloro nsthane- 2-ciiloro ailyl alcohol ancl n-acyl cbloride* All but the

last methionod iimtcjrial killed 100:2 within J$ hoia-s after traatnsnt \d.Jch

dosages of Isss than 10 rags» por liter* Tl:o snpicsclsmte points of 95/5 killaftez*-4vS hours •■rerc 1»?3 /ja3, 5-»7, 5^S s.nd 56 irilli'-'rair-s per litor,. respective

1^ 2«-Sitroao pontana was approrcir-^toly r.s affectivo as its prop5.ee hoitsologuo

against the larv<?.S; and more effoctivo tr^.-.i 1.2-&i.hpo^o butaiio* Against the

eggs9 it vas nuch less effective then any of the lo'.rer I;o-:ologuoa»

■c:oic"3j.o:;c o'theme tjas El.lgiitv.y r.'.oi'S offective 'Against layvae "b

broino trichloro E

2~chloro a!3yl alcohol -fas less ofr'ectivo than £^3.?.yl alcohol against both

©ggs and larss

ethane sho-red very little toidcity, iilie^eas l?2"di.bi'oa!o etliane

ditooaddo) kills all eggs or.d larvae at less than 2 E?gs« per Liter*

\

Page 5: (Formerly Oriental Fruit Fly Investigations0

-182*

Table X* Materials aoreened as xumigants against naked eggs and 3?d instas? larva© of Dacus dorsalis

M ATEH1ALS

1,1,2> S-tstrabroEO oi

1,2-rIibrcnso cyclofcsxone

."L-'bri'CToo ethyl bDnsea©

2-»>roj^o ethyl bsnsene

Ohlnr;ldes

S-bthylcne. chloride

1,!j2"ti\ic:hlo3?o ethane

1 ? 1.1 j 2-tst^achloso pr

X, 13 2,2«3-p3&tacfcloro propane

n-bi^.'ty.l ehlorlcbhloTo butane'

u^rl cluceid©

1,S^&ichloro peatanoCyclohorr^l cb3,05^:lc?-G

,'i-obloro allyl -Cvlcohol

jl £D«50.

'*• lV"i

?131

ii

>153'. >136

I! ■ loo,

>123*65

•>176

>90

>106

"2L04

29

2A hours houra

>90

>.173

6550

5235

5

>106

3o00

>150

>123>135

6

>no

73

(cont'd)

8«0 j

0«601

173

>X53>136

66

i >135>176

>no •

42

>10690

2-1

56

>106

>173 i

Itortalities (^) athighest dosage test

ed (O«1O25 mlso pe2liter• approso 3/4-

lafter ZB hi*s

46

10

0

16

32

9

10

do

1352

26

20

4920

2612

24

10

100

0

3

3

231

96

93820

7433

100

18

55100

Page 6: (Formerly Oriental Fruit Fly Investigations0

Table 1 cont-d

Mortalities \yj afihighest dosage tost* ]

ed (OclO25 lifts* par |MATERIALS

Chlorobromldea

Bromo chloro mebhano

D dichlovo nathano

Bpomo tsrlcblopo ethane

lp2»dibranio■*!,!, 1,2-tct-raehloro()

GH2CI ™ CF^CX

CH Cl2- CP2C1

>no

>92

Acetic anhjrdrideEthylsn©

AUyl iaotbiocyanateEt%l other

Me^yl ethyl ketoneKetl^l

n«propyi alcohol

i-propyl alcohol

n«=buVl alcoholi-buiyl alcohol

alcohol

^ 25 grams pos

Page 7: (Formerly Oriental Fruit Fly Investigations0

■^g^Qjff.^ determine tho effectofjlqad on the survival of D* &P.ggalig. > * TSilock~^"S"J~

A total of 57 tests was conducted t/ith EDB and papayas« Iforaally the

fruits ■were field-infestad, but in many instances thi3 infestation was

supplemented by es:posure- to caged flie3 to build up fci^ier

(2)

Tha teats ware confined almost entirely to determining the relation

of fruit load to fruit fly mortality* The preliminary rtudies were con~ductsd :la tha 19«6 liter friction top tins used for fumigant screening*By varying the fruit .load using 5 and 10 papayas equal to approximately

1/4 and 1/2 tlie free air space in the tins, it was faopad that differences

in adso:?pf..ion trould be reflected ±i nortaiiiy curves undar both loads=>

;uLso by including naked eggs in &>ti'i dishes in each tast, *&e rate of gas

adsorption by the fault would Ids .Teflooted :ln -fclio egg hutch* (tabixs 3)

e 219 !• (7o7 cu<> ft«) water-seal iron dzivan vjora latsi- substitutedfor tho Gcxvsening tins in these stvdisa* ?if-^;-i:lv3j HO and 165 papayastrai'e fusaigated at one trine, a voJ.vss cqinl apprc~h;s.x,olir to 1/4., l/2? (2nd

3/4 'cho free air space in ths dvon. i\n 3" fen placed c.t tho bottom of thedrum \n-z opiated foi* tho fi::-st 20 ninutec of e?qpo3ivi'a to provide cix*c\jla«»

tion* Tiie fruit'vias placed loess on a scvooncil rZ.r.t-Syrm juat abo>re tha fan»

linked eggs 2A hours old yore fuiiii^.-.tcf.l GimaTtxaeouciy in opon Petri dishes3.iaed u:'.th sioiat blotting paper (1C0 ec~^s bo c. dicAi). T-;o dishes irara used

in most tssts one iraii'sdip/feoly above the :cru:".t eziC. one b'3lo:-;» Diffarencs

/P^ in hatch due to position x«s not cpjrcirsnt« TIao fiud.gxtiug cont?.ins?s '.-.'arev 3aainta:L:ied at 70° F, in the 240 cuTft» tI13rr.ostaticir.lly contro3J.ed chambero

5:hs ED3 was applied by irlcvoturetta onto filter paper o Volatiliaatiou was

complete) in from 7 to 12 minutes depending on -Jihe dosage usedo This xms

fcid observation through a glass viadoij cut iia tlia cover of the

equal to 1 agA to 0«>4 nlo equal to 4 mg/lo 3h the 19«6 lo screening tinsC.05 ral» of 10JS EDB in CC1. was equivalent to O»55 sg«/lo of tschnical EDBc

Tha date obtained 5jj these studies are not extsnsfara enougli to draw

comparative taoi'tality curves, biit they reveal some interesting information»

As was oxpacted there \te,3 sons increase in survival as the fruit load uas

jjicreasads Th5.s vas indicated both in the results of survival froti infested

fruits fis well a3 from the tests irltii naisd eggs^ At tho .lots? flosages testedit appoars that naksd eggs are nuch j^ore resistant to funsj-gatlon than are

egg and larval stages in fruit» At tha loijest dostigs 'Sosted (O055 ng^/le)tho mortality in the frii5.t uas :?ror. 2 to 3 tines th?.t shoia foiv naked oggso

This w.:i not erpscted sines naked e^gs v:ors c::?ossil directly to tlio furaigant

x-ihereas eggs and larvae in fruit voro afforciGtl fjoiio vrotsction frora directand issndiats contact with the "fundgimt. II73 st'ssoctod o:-ol»-;nj?.t:lon is that

this resistance on the part of the egga is ;r.ov3 apparent t:i;'.ri real, end doesj?.ot tai-.- iato accoiuat tiio clalaycci lotl'ir.1 action of i'B3 \?hioh .iay psrait bs.tch

but c?.u;:o "Lltimats death to the yevng la::--ae bsfors thiy ere able to completethoi?.- clovelopiEsat 0 Thic is born out by tha results at tha higher dosages

where ona trould e:-:psct ths lethal action to be accelerated* At dosages of

2 inga/i" and above tho trand is apparently reversed and iaortallties of nakedsggsare generally higher then iaorta!?.tles of tho inanatiire stages in fruit 0

This sens apparent diccrepancy in- results \:ith EDB has occurred previouslywhan it wa3 reported that LD 95 fez* nalced eggs was 1»5 rag»/lo (tj«. 203 last

Page 8: (Formerly Oriental Fruit Fly Investigations0

—lop-

The mortality of the imnaturs stages of the oriental fruit fly inpapayas fumigated with ethylene dib:?oraido xirder varying loads and

at various dosages for 2 and 3 hours at 70° F»

Ezpt«,

Ko,

169^/170 .

1715/172

173

174175

176

177

178

279ISO

181

1S2

1332&£

185186

1871S8

189190

191192

193

194195196

197

138

199 ■200

201(1)201(2)202(1)202(2)203(1)203(2)204(1)204(2)205(1)205(2)206(1)206(2)207(1)207(2)203(1)208(2)

Type of

fumigator

5 gal, can

5 gal* cann •

n

n

8

a

n

n

t:

R

o

n

n

e

n

n

n

f!

n

Xi

n

H

n

0

a >

0

n

n

a

a

n

n

n

a

a

n

n

H

• U

R

a

Tl

a

Dosage1/ZBg./l.

1.15

2; 21

51551,11

1*66

2.23.

O..55

1,11

1.662O21

0.551.11

JL66

2.21

0.55

1»11

2B662., 21

O«55- 2,11

3*, 662,21

0.55

1,11

1.662.21

0.551.11

1.66

2,21

Oc55

0.55

1.11

1*11

3*66

1.66a, 212.21

0*55

0.55

1.21

loll

1*663*66

2.21

2.21

Esposurs

llOUi'S

2

2

2

20

i2

2-

2

2

2

2

2

2

2

2

2

2

2

2

2

2

22

2

2

22

2

2

2

2

2

2

2

2

2

2

■ 2

2

2

2.

2

2

2

2

Wo* of

fsuito

5

5

3

510

3.0

10

10

10

10

10

10

10

10

10

10

10

10

10

10

55

5

55

5

55

5

5

5

5

5

5

1010

10

10

10

10

10

10

Eat.

pop*

1255

1255

1255

! 12551255

1253

205.1

205:1

205X

2051

2051

20512051

2051

183152838

1838

3B3S183S

1838183S1831?ssc-

out;

Si>6

S36

8S6

156S

156li15681568156£

156J5156B156U

1729

1729

172917291729

1729

172')

1729

Survivors

pupae

0

15

301

0

0

0

56S

102

35

•4.370

5S0

0

7840

0

0

233

92

0

1030

■0

0

"".902

S978

460

0

16

130

655443191

1343937

0

17

adults

3

46*

311

25*150

245»

"33

459

273

2

0

0

• to

S36

2

4?23

d

0

0

4.911'7B

79

496

19

par.

0

|

sort.

3X0.0

98* SO76u02

100c 0

100.0

100.0

72.3195»O3

98e2999.80

8lo96

97.17

100*0

200oO

57o34lOOoO

100.0100,0

87.32

99.51

99.89

?.00c0

S3, 37100.0

100.0

100.0

02,239S.5S

100,0

99.77

95?0297.07

100.0

100. C

96,. 98

99c 17

100.0

62,12

74,37Q&.9592*25

97.7497.36

100,0

'* 1

eatoedegga

74«00

100,0

6»0

92.0

100.0

100.0

1S.0

30.0

90*0

100.0

10.0

70.0

96.0

100.0

43.0

92.0

lOOoO

IOC 0

45oO

81.0

95.0

100,0

4*40

200*0

10040

6*50

88.71lOOvO

lOOoO

460 0

70^0

94»0

98,0

100.0

200.0

100.0

200.0

56.0

44a 0

9200

90.0

100.0

100,0

100,0

200,0

Page 9: (Formerly Oriental Fruit Fly Investigations0

Table 2 Ceont'd)

Type of

iunieator

209^4^)5 gal.

-2(a).j "

-3 (a). 1!

=4(a) B

—5(a) n(to)

210 7,7£fc^fis211

212

nu

it

213 t!214215 .216§/,217-5/

21G' ■

219

220

222

233

224

225^

Chocks

u

H

°

n

tt

V

H

1,11

1,11

loll

:lii

urn 2»0

2«o

2,0

4oO

4-0

4,0

i;o

2,0

2«0

:uo

;2co

2,0

240 f-h? ctexulZ.,0

169-lSi.

135-200201-202

209 Or.)209(b)210-215

217-224

225 |

1.

r

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

3

Ho, of

41 v4i^414/

4U-4j

|)3

4(k•55110

165

55110

165

55

55

110

110

no

400

]X5T>4

262

1430

262

1430

26J

1430

26:-.

343026:?.

3430349'r

6994

1049:?.

3497

699410492

3546

364«S

3646

729:.'.

729.1

7291

999.";.papaya

A5?. "H---

432 »ii

JiO

20

6510

10

165110

100

• •

_•■■ • .>

.1. '. > "f- *s **.r

7291

249.:-

S

16

61

132

3139

95

591

109"30

S3

0

0

15

. 93

13

0

967

136

■ 229

0

13350

3575

7291

adi-lts

2

7

626

. 511

2A

0

14

" 20

24

3.76

0

0

5

26

2

573

35

9 ^

-17>?

5474

lr'19

par™

0

0

0

2

2

90

0

0

0

?c

0"

Ct?.pB

2

4ft93»9

95.749c 697.3

85,1

93.477© 5

99.9

58.499,3

lOOoO

98*33

97.1

100*0

100.0

99,36

97.45

99.64

100.0

86,74

98ol3

96US6

100,0

1

!

©ggG

.™»

100.0

lOOoO

B.49I710a. 0 ■

100,0

lOOoO

B-2619

B-93.3

1-942

B-aQO.0

"£29.5

A-9&7B-2QQ0

■<-»iFciO'«icts!3 en. follouisg tags)

Page 10: (Formerly Oriental Fruit Fly Investigations0

-187-

Table 2 (concluded)

* Raeord incorapleta

3/ Tech, EDB» Diluted to 10$ uith GCl^ for aaperimsmts in 5 galo cana.Undiluted material for 219 H'fer drums aad 2^0 cu, ft» chaJBbor« Dossg-3S

giTren are for toah&Lcal EDB»

2/ Based on piroal sn.xf7.ivo?Sc

^/ Eggs exposed in opon Fatri dishes (100 ogga each tsst) balou fjpuifc loadunless otherwise :bi'lioat3a0 (A)=Above-rsasitj (B)=3elcfa-fniito

y Six replic3bsss (tt)==£jcuits e:5p03Qd in cages to D. dpgaaJ.g.g for 4and ijronsdiat93,7 follouing for 2-1/2 hours to 0o oopSaJ.^ at the TBoard of /igricultee €z Forests.«y«, (b)=frtdtB cage<-infe5ted id.th

^/ Data discardad baeauso of Isalcago or uncertain dosage^

6/ Pineapplaa yrappsd :i.ndividTsai;iy in eonnxgatod papss* 3x. woodan eratos12 s 13 x. 2GR (36 crates e 12 fyuits each)* Pap^as were placed iufield baxea<«»20 :?icuit3 per bccc Fsaiit ia er^aj?y other box -i/as la-apjied

in corrugf-tsd pepe:? collars; otherB wora nakede The papayas uexe plac>id

in fch.6 top sni bv&tom layavsji the pineapples in the 3 roiddls layero.

Page 11: (Formerly Oriental Fruit Fly Investigations0

Tablc 3» 'i'ha u \""oal:Vfcy of dorsalis ©ggs and larvae in papaya a»jfi to nalced

3ggt: :bi opan Pefcri dishes \ftvan fumigated taiclor varying fruit

loafe :*<y/2 hovirs c« 70° ?» with BDB at dosagss of 1,11 to 4*0

Big-A'

1

19=6n

I!

n

it

B

K

n

219 :Tl

:i

it

it

(i

j cf

L»d2

i>

i:

i;

. I!

i

J

I

■i

n

n

n

n

n

n

uta

I-Io * ;

toS u3!!

r>

5

6

6

1

^

3.

■^

1

^•..•uiLt 3.oad

3

u ■

:lo

;lo

.10

10

55

55

!

:ao'110

■ V('C

:i653.65

i

_Vlo/l

0.55

lill

1.66

0*55

X.662-21

'l»0

2oO

loO

2*0

4.0

2*0

AaO

.^st. no» eggs

and larvaa i:i

fruit

61636532

7418

11236

112361123611236

3646

.107S93497

729121576

6994

IO492

10/.92

723

55 •■?6

30

3053

49411321

93130

967l./o

0

304

15

88.299.2

990'?99-6

72-.S29«5 ,.6:3

98o99

99*81

37,4599-83

100*00

36.7/,97«92IX.03

97*10

99.8S

! Haked

500

500

500

600

600

600

600

600

200

500

100

200

5C0

100

200

1C0

%

vSSy o_5

IOOoO

100oO

36aO

7604.96,8

XOOoO

39*193.3

100 aO

— -i i99.1 I100,0

92»05

IOOoO

Page 12: (Formerly Oriental Fruit Fly Investigations0

-109-

quarterly report) and 1«25 mg«/l« for eggs and larvae in fruit (p« 662 reportfor Oct»'~D3e» 1950«) Although data from naked eggs may be misleading as ameans of determining dosages requix-ad for complete mortality, this methodappeal's to hava considerable value as a bio-assay method for determiningsmall differences in gas concentration during fumigation; sintje us have aochemical Esthcd praciss enough for accurate determination of jsmall amounts

of EDB»

When one considers the very high sorptive rate of EDS, end tha limitations

this imposes on'the effectiveness of this material, it becomes increasinglyapparent from our mortality data that only very minute amounts of EDB can bo

tolerated I^r fruit flies*

The possibility Sag been suggested that parasitised fruit f3y larrae are

more resist&at 'bo EDB fumigation than are taiparasitised larvae (p« 193 reportfor last quarts:?}« Expsriiaent 209 in the prassnt series \i&s designed to further

test this "possibility o* Bipe papaya fruits were cage-infasted by JD» aojraslis*One hsjj? the fruita iiera exposed to Q* ooghilas tho s?iue &£$ for 2 l/2hourGoBoth lots vjsre 3ield for 6 days sjicI then. fSiigctsd in the 19»6 lc acreeaing

o Foxh* parasitised 2nd 4 non-parasitised fraits -jsrc fusjigatsd at one

with 6 .^plications, all at a" dosage of 1.11 Kg;,/l» In each tost the1 hih bi itid fit h

p

sxirvivf.1 \:&b higher in biJ© parasitised fruits as shov-n bslov;:

Sable •!■« The e:rf*3ct of paraaitica hy Q* assJliiHa on D.L do^sjiulis. larvae &21

pa^syr?. fitraigated i-iith EDB at Idl iag*/l° ^>r 2 howra at 70° F»

MIX'

1

2

3

4

5

I

BfiBa—.

262

262

263

262

262

1572

^^JSuryjhTgl

16

39

59

109

73

2

2

93 »9

4.9*6-

85*1

77.5

72.3

72,S

U30

34-30

3430

H30

1430

Survival

31

95

1

0

191

Z

9

21

1 99"9I? 99..S

!iIi 3.00*0

\

The su5."7ivi?.'> 5^-. parasitised' rr/iorial 5.9 consistantly Mghsr Iv. each

and thsra ap;3s?.i"£ 1;o 03 quits a prono'oncsci difforonco bet-jeon tha E3an

niorfcal&tias, 72 »8 for tho paix.siti:;od natorial and 97=S for tlis ranparasitis

Hotievsr, thsfis rssiO-ts are not substantiated by ths parasite ossrgenca f:?oa

the tvro lots» Oaly .2.0.cj?jn}ij3m' cnorged from the cages parasitSsed 2sat3r:lal

and 11 oopMlus frc-a ths othsr 3.ot. apnarcntly lsaraeitiaod in tho fieldo

Page 13: (Formerly Oriental Fruit Fly Investigations0

It iias possible to run only one large-scale test (Expt» 225) in the 240ctu fto chamber to determine the effect of packing material on fruit flymortalityo This experiment was conducted with pineapples and papayas

■wrapped In corrugated paper collars» The fruit load totalled 23&L lbs a,4.50 lbs« of which ware infested papayas. The pineapples were wrapped incorrugated paper and packed in 36 wooden eratos 12 x 13 x 20", with 32

fruits per crate o One-half tha fruits -were plant crop and tlis other half ^h ill d ith ll dp p

ratoon h-v&tso The papayas were similarly wrapped with collars? and weseinterspaced through the load to determine mortality under these conditionso

The fruit fly population in the papayas was dot3rmined as 9*992 eggs and

larvae and ao survivors wre recorded. The dosage used was 3/A lb»/l000 cuoft. for 3 horn's at 70e F» Papayas exposed at the saise titns for tolerance

showed no ill effects frcEi this trsatrasiit as reported by -Akesaina* The entirei E

p y

load of pijaoapple was turned over to Akamane of tho Hawaii Agricultural ExpQpi-msnt Station after fumigation- They uor-3 held for 6, 11, and 17 da;>-s at

45° F« and ths:.i e:-:?.ndnerl daily for 5 days while bcin'g hold at room teispsratttre»

ihsre was no indication of injury cxe to fundgation, a-id ther-s vraro no detectabledifferences bsti-jaor. ftnaigatBd and control fruits*

The results of 13 tests coaductso; to date to determine the effect on

fruit fly morality of different sathodo of pack are suranariaed in table 5«

The methods of pac&isig under tost were: ..

1» Fruita naked in fiel-3 • uox^s-

2* Fruits nalced in field boxes lined with newspapsro

V . 30 Fruits wrappsd individ«a3Jy in corrugated paper collars in field boxes*

Jk.o FruilS «rappad in ci'<;?:!?.s tissus in .field bojses*

5o Fruits packed in wood or paper ejccslsior in field box-as*

6« Fruits wrapped in corvi>2i'.tod papor collcrs and sooled in cairdboard

cartons--

7" Fruits -.Tapcsd in citri's tinsue v:a<l pacl-cecl in o;-:cel?.t'-o:.* in so'iled

carcibocrd cartons.

Thece •beats shewed thsit at 1/2 lb./l(300 cu. ft. fpr 2 hours mortality \..>ascomplete with nc-Jrec: fruit and frait wrapped in citrus tissue in £ie3.d bases«

When tho field bo::es wDra linsd wiv,h nowspap3r the norta.13.ty based en i-scovered

pupae was; redusad to 99»31?j» When corrugated papsr co3J.ars wai'a placed around

individual fruits the mortality was 99«96jS at 1/2 lb./2 hom-3 avid 99«9S?JS at■ 3/4 lbo/3 hours * With this 3.attsr treatmant 20 pup-aria, were j.-scovered from

ari original fru5.t fly population estimated as 164j237, but those failed toproduce adults, probably indicating delayed lethal action* l&sn fruit was

packed in e^celaior at 3/4 lb«/2 hour dosage pi'oduced 99'92>S morality andat 3/4. lbe/3 hours, 100$ in the siJiglo tssts cond\.ict8d» High moralities,99o^, irers obtained at 1/2 lbo/2 hro. with papayas packed for shipment in

• sealed curciboarcl cartons» Gorrugatscl paper collars only, and citrnn tissue

r. virap pif-jj excalsioz1 pack -fsro used with tho papayas in the ces.lsd cejrtpnzo

At 3/4. lb/3 hoxtrs morta3.ity was complets in bno test when 11 sealed cartonswith 108 fruits wr&ppsd in citrus tissues and packed in c::colaioi* iisra fumigated at ths sojss t-3H9 as 1260 fruits packed in field bores with accslsioro

Page 14: (Formerly Oriental Fruit Fly Investigations0

-191-

Table 5. Sumraary of large load tssts with EDB in the 24-0 cu« ft. fumigatingchambers under various methods of pack, and under-varying dosages

at 2 and 3 hours' eacposure at 70° F.

/pN

Test

155

158

154a

Dosage

1/2

i /■">

1/2

1/2

15S

157

160

161

1/21/21/2

165 116716S

3/4

(Fruit 1

1/2

135

166b

164.

166a

1/2

3/4-

3/43A

Hours Load

2

2,

11511596

12580

.JZS69.

0

0

0 100

I 1596| 20

A()0 ,0

99 .-.31

2 100 O0

Ba2S£-fi2^SSa^LS.iS3^»SPJ^)

2

2

2

2

1005

1582

1596

*iL*—-«^.-i

3 136S

1363

1180610890

198644919

0

0

2

15

13^996

0

12

0

0

100 *0

100 »0

99«9999o70

0

0

0

0

59^6"

100=0

99o9?li 99-95I 100,0

A00 \ 7428 13

Cferdboard

2

3

3.095 4949

1S03

9 0 | 99*

0 100,

2 1368S 1369

3792

21037

30

0

0

I 99*92100 «0

1/1/2 of .-fruit tn?ap>3ed in papsr collars (Bxpu« 154b) cnct J./2 :l-ii crszus tissue«2/IDo dorsaligj jfb* longicayfig.tus • .2/ 10S fi-uits ii- sealed bos»s, rsroainfier 3n c":ccXg?.oi- in field bosceo (E:cpto 16ca)

Page 15: (Formerly Oriental Fruit Fly Investigations0

-192-

It would appear from these data that the" use of packing material at the1/2 lbo/2 hour dosage would involve sons slight risk of fruit fly survival..The combined results with this 'dosage for the various packing methods were 69pupal recoveries out of an initial fruit fly population of 96,961, but only4. adults emerged and one of these tjas the parasite 0», loppjcaudatuso At the 3/4lbo/3 hour treatment, 20 puparia were recovered from an estimated pre-treatmentpopulation of 187f,077 egga and larvae, but these failed to produce any adults eTaking a conservative view, pupal recovery could be interpreted as being doseto borderline survival even when no adults result* Reconunsndation of sucha treatmsnt could be made with very slight risk of fruit fly survival in acommodity such as pineapple, which can bs classed as a poor fruit fly hostwith a recorded infestation index of less than one larva per ton of fruit•This figure is based on holding tho skins of shelled fruits, -which methodincreases chances of larval development and can therefore ba considered theextreme ind-a;: for the 156.4 fruits uaed in these studies over the past 3 years»

There v.-ers surprisingly slight differences in the effect on fruit flymortality from tha aix widely different paciting nethcc.3 tested. Mortality inpre-packaged papayas in sealed cartons was alnost as high as with ths other

methods under testa The depression of mortality by lining field' boxes withnewspaper is difficult to explain, especially \&6& .compared with the excellent

results in sealed cartons»

Over the past few years there has bsen a steady increase in the export of

fresh Hawaiian"fruits, especially papaya and pineapple, and more recently afew test shipments of vegetables have been tnade* This growth has bsen closely

correlated with the fisvolopnient of successful treatments for fruit flies«

From May 194-6, when B» -dorsalis. was discovered in Hawaii, there were no shipments

of fresh fruits or vegetables until 19/<S when vapor heat was approved for papaya q

This treatment was later extended to "coil pepper, Italian squash, tomatoes and

pineapples &fts:e necessary tolerance stud:;.&2» Kothyl broaide for' pineapple

was approved on April % 3.950, end on April 2/;., 1951 ethylono dibromide was

approved for tha treatiasnt of &.vo3ao.o} ball peppo?, bitter Ealon, Cavendishbanana, cucuriber, papaya, pinea^le, snap beans, s.nd Italian squash* The

effect these development0 I22.V& hiid on ialan-3. esportcs is chovjn in figure lo

Go Ethvlono dibi-c^da© apji^fup-igait "0^..y^g..):^g.4:VJ.3.^?v:ir3a

is sxpariiaents (72-97) \we coEiplotsd in tb:'.s Kori.-3s to dateunineeffect of ethylsne dibrcrdc'e on the i!b^!:lt3rrancan fr^lt f.ly in oranges»

Four o? tha ezporimsnts vrara coaductsd with tangsrineo from &aui -w

ware rspor'oscQy hsavily infested by liadfly. Raymond Miyab&ra wa.-s kind enough

to make arrangements for purchasing and shipping the fruits to Uonolflus

Tho fruit fl;.' infestation in tfcass fi'uito was rather light, ave'raging lessthan one larva po:r fruit snd tljis was predominately_doj^lis with c^g&tata.

making up about 2o5 i.er cent of tbe infestation*

Page 16: (Formerly Oriental Fruit Fly Investigations0

-193-

2,000^0 30

l,500r0 \Q

1,000,059

Figure

5OO9OOp-

The effect of recent developments of new treatments

for fruit flies on the export'of fresh Hawaiianfruits and vegetables« (Data from K« L» Jfeehler,Div« of Plant Quaj?»)

Treatment Date Commodities

approved- cpveregL

Vapor heat

ZfeBr

EDB

Apr«30s 194S papaya0cto!9# 1949 bell pepper, Italian

squash, tomatoes

Dec©10j, 1950 pineapple

Apr* 3P 1950 pineapple

1951 avocado, bell peppsx*#

bitter melon, Cavendish

banana, cucxaraber?

papaya3 pineapplef

snap beang, Italian

iocr-hsGt 30%70$IfeBr

Vapor heat (100$)3,240 lbs*

1949

Vapor haat 10%

MsBr 10%EDB • .

'rr^-r^rrn{rrfrr>rffrr^!^v^ar^':*^^^^'s^^^

1950

Fiscal Years

1951

Page 17: (Formerly Oriental Fruit Fly Investigations0

/JB\

The remaining tests T>iere conducted with California navels which wareexposed in cagesto eapitata. Tho resulting- infestation was disappointing, aver

aging less than one lasva per fruit* Again various nsans for increasing ovi«=»position in the oianges were tried, such as rolling and puncturing the fruits,but at least in this series there was no advantage of rolled or punctured fruits

over those x^hich were exposed as purchased"

We are experiencing difficulty in isaiiybaining the stocks of MedOy re

quired for any extensive studies. The flies are difficult to Ice so alive forany length of time, necessitating constant r3~stoc!dLng. Because of the small

numbers of fruits it has bssn possible to infest under these conditions it

has been necsssary to conduct soeg of thesa studies in the 5 gallon■screeningtins* Data obtained from the larger water-sealed cbaub-ars of 7*7 and 10 cu» ft*

should ba more accurate and more nearly ropressntafcivo of cosmsrcial fumigation^

and we plan to confine future testa to these chambers»

In the present series of tests the fruit load was maintained constant

at 40 fruits for the tests in the 5 gallon cans* ^his represented a fruitvolurns equal roughly to 3/4 the free air space in the 5 ga3J.on cans« Dosagesused ran from 1 to 8 mg«/l* aqual to 1/L6 to 1/2 lbo/1000 cu« ft« Exposurewas 2 hours for all tests at 70° Fa Survivors i^ere rocordsd only at the lowest

dosage used;, l/l6 lbo/1000 cu» ft»

la. the 12 experiments conducted in the 7*7 cu« ft» steel drums ^ath water

seal, the fruit load was kept approxiEate3y constant at 2 cu« £b»9 but the

fruits were packed in three nays: (1) 12 wooden trays -with screen bottomsi(2) in a cut down orange box 12" :c 12". 2 24"3 (3) ia tvio burlap 3acks« Dosagesused vsre 3/L6 to 3/4. lbo/1000 cu. ft» for 2 hours at 70° F» Survivors warerecorded only at 1/16 1b*

Detailed data are shown in table 6»

Page 18: (Formerly Oriental Fruit Fly Investigations0

-195-

Table 6« Mortality of tiis immature stages of the IMiterranean fruit fly and tho oriental fruit £3y in orangesand tangarines fumigated with ethylon© dibromide at dosages of 1 lio 12 mg«/l* for 2 hours at 70° F«

310a

2/73

742/

76

77

7S

79

SO

81

84

85

86

87

fumigator

dram

n

n

5 gal* can

n

n

Doeag© ing«/l

2,0

2»0

loO

2,0

8o0

hctsr

2

2

2

2

2

2

2

2

2

2

2

420

I 420

193

204

40

40

40

40

40

40

40

4-0

40

40

40

40

40

Kind & Source

Tangerines, Makawao

Maui

Tangerinos, Haiku

navels

con*d

155

£55

155

155

100,

100,0

lOOoO

10G«0

98*06

} 100oO

00. |

0 ! i0

0

1

100 oO

100 oO

100 oO

100*0

100*0

Page 19: (Formerly Oriental Fruit Fly Investigations0

Table 6 eont'd

no?

90^

93^923/932/

9^

973/

!typa of

fuznlsatoy

7e7 CUefto

drum

R

n

n

n

. »

n

it

Dosa^a Kif&/1©

2*0 i

;

, !

12 oO

1,0

4-0

1

Exposure

hoUTO

2

2

2

2

; 22

I

Ho© of

fruits

273

273

273

273

250

250

250

250

210

102

3791276250

Kind & Source

Calo navels

ti n

tl H

n n

n ir

n ft

n n

si n

Cal<> QEV61&If R

R 0

tr «

Estimated 'popiul

ii

1

3J.7I ^»

0

ation

Mod*

201

201

201

201

2S9

■ 289!\

-3

155201

Survivors

0

0

0

0

0

0

0-

0

i" " *5/1' 02i" ;5 4

[155!201

1

adulte

dor*

!5f 00

0

0

Med«

2

•wo

3126

3492S?

Ps? cent •* /

mortstli-oV*"^

ICD^O

'lOOoO

100«0

100^0

100eO

1QO«O

100,0

1

I

i

%f Based on pupal survival*g/ Fruit fumigated in 12 wooden tray© with wira bottoms, 2-l/2Ksl2ns3.6ny Fruit placed in burlap sacks « 2 sacks psr drum«

Emit packod in cut down orange box 24-" x 12" s 1211No emergence datac

S/ Field infestedc2/ Cage infested by capitata.

Page 20: (Formerly Oriental Fruit Fly Investigations0

-197-

(0) Sorption of EDB (Balock and Swanhota)

On March 24P Carl Swanholm, a chemistry major at the University ofwas employed to assist on the problem of sorption of SDB by various commoditiesand the various surfaces such as walls, packing boxes and materials which maybe involved during the process of funigation. Sorption of EDB in a metal-linedempty chamber of 240 eu. ft. capacity as determined by uLtra-vlolst absorptionwas found to be as high as 37-3/2 per cent and with papaya in field boxes occupying 1/2 to 3/4. of the volume of the chamber the loss approximated 75 percent after 2 hours from an* initial dosage of 1/2 ib./lOOO cu« fto, apparentlydue to adsorption. (Rept. Apr^Jwie 1951 p. 82?)« Lln^-ran and Sinclair (1951Jour= Econ. Ent» M(6)s 980-990) reporting results from a 100 cu. fto gas tightmetal chamber stated that from a dosage of 1/2 Ib./IQOO cu. ft. 52 per cent of thegas was recovered from an eapty chamber after 6 hours, and 8 per cent and 18 percent with oranges in field boxes occupying 38 cxv ft- and 19 cu. ft. respectively*Tho method for the determination of etbylen© fiibromide ucecl by Liafigren andSinolair was reported recently (1952* Sinclair and Crandall Jour. Ecozu Ent«

_. • _ _» * _____ ________ _. _.. *w ^ m « t m ._ ^ ^% . _ _ % _P _ V^ _^ ___

tarminao^^ but for EDB tba gas eamplss MJst bo Loafrad in a hotels? ovenat 90° G. for 30 minutss for the roactioa of tho EDB with aoao . .noihMno to

Using the above method for EDB determination a series of tests was conducted

to determine the rate of adsorption in tho 7«7 cu» ft- iron drums equipped witha water sealo The EDB v&s introduced at the rate of 1/2 lb«/l000 cu» fto witha micro-burette graduated to o05 ml<» Three mstliods ware ussd for volatiliaing

the gas* (l) The burette uas intreduced through a hole in a rubber stopper

and the EDB placed on filter papor to increasa evaporati surface» Burette

was vathdrawn and hole tjaa stopped imnvediatslyo (2) Sana as above but theEDB was impinged on a hot (?300°C.) porcelain crucible so that volatilization

was affected i..iaediat3ly» (3) Suse as (2) except tkat tho burotte was leftin place to maintain a complete seal throughout tho exposure. For comparison

one test was conducted .with I Br at 3-1/2 ibs./lOOO cu. ft*

The results shcired tbat undo:.1 these cor.ditiono in e.r. esroty, uapc?J.nted5

ge.s«tight iroa dz-vm cf 7»7 cu. :'?-o. cr.pac?.^ tho Iocs of EDB waa 4-0-5? per cent

after 3 hours fi-on an initial dosage of 1/2 lb»/lOCO cu. ft» With MeBr at3-1/2 Ib./IGOO cu. Tt. thg loss \:s.u api:-ro:::*n:-.toly 30 per cent in tho sses

period. Thooe losaey aro attributed to r.cr:orp"iiic:i since the finas uere tasted

and found gas-tight =.

The results are shown graphically in figure 2»

Page 21: (Formerly Oriental Fruit Fly Investigations0

l/2# EDBA000 3Figure 2a Sorption of EDB in Empty Drums (I£on)*

Absprbtioa O/-v0 Methyl bromide - 3»5 ibso/LOOO £t« ™ EDB added through hoi© in

stopper*. Volatilization not

instantaneous •^ EDB added through hole—possible

loss* Volatilization complete

instaQ tly*

^ EDB added in sealed container oVolatilisation complete instantly o

sample of each curvo may have

an error of » 5^ due to air insampling

SO

Timo - Minuter

160

Suanholm

Page 22: (Formerly Oriental Fruit Fly Investigations0

-199-

Line Frojoc/o X-»o~5"?-» Investigations to Determine Infestation Indices la

Eibli^55iH^^^^rd Vegetables- (Balock and. Kosuhs?) '. ' "

Thia?ty~four lots of fruits and vegetables t/sre hold during the current

quarter to determine the degree of infestation by fruit flies*

Sines.these studies ware initiated 12 different kinds of fruits and vege

tables have been held to determine tfcsir susceptibility to fruit fly infestation.Total collections *?ere 428S comprising a total of 26j924-paund3« he commodities

represented are for the most part those for which treatments have been developed^

with the exception of egg plant.

Data for papayas and pineapple, the feo main export crops are more ex

tensive than for Eost others, and sake up about 2/3 of tiio total fclt

craarter zlcs friiits avsi'ags noar?,v 5 lar-.?ae psr ISO lbs. and 1/2 i-ire fi-uits

S5.

Pineapple has one of -$hs lovroot j.-SGcrclcj'. indicsa of infe,otst?.on, 2 larvae

•Jii 514i8 po'oncis of frouilt, and -jhon one conrjilo.srs tliat this i7£,/? iH2corded in

shelietl fruits- ifhich incrsasss con;sic"ore.'cly tho cin'.r.css for l?.i-Vi?.l survive!, .it sesEs quits evident that piiisapple is a vei-gr poor dogBaiy^i host».

able ? Ir^edia-osl;/ following .suraisarifjas all available data on aafestation

records* Datailed oxits. for the current quarter e.vss shown Itj table So

Page 23: (Formerly Oriental Fruit Fly Investigations0

-200-

V.Table 7o Msan indices of fruit fly infestation in fruits and vegetables*

j0

Fruit or Vegetable

Avocado

AniPm (Cavendish)

Bell pspper

Bitter melon

Cucumber

Egg plant

Mango (Haden)

Eapaya (solo)

Pineapple

Snap beans

Tomatoes

Zucchini squash

Totals

Degree of

maturit?/

me green

mo green

1/4 ripe

eu green

1/4 ripe2/2 ripe

m» gseen

1/4 rips1/2 ripe

1/4 - 3/4 ?S

e» grass

1/4 ripe

No* Of

collections

45

7

2

32

7

25

12

445

5940

30

.pa 78

16

26

26

8

428

Eoo of

pounds

1695

31580

"1212

275

2399

267

U

1511

737423141934

5148

413

14511235

215

Index

Larvae per Ibe

0«069

0

0

0*223

5.822

1*559

0

Io55

7«93

OoOl

0«0450o850

0,00038

4*37

0*595

Oe853

3.01.

Page 24: (Formerly Oriental Fruit Fly Investigations0

Fruit fly infestation in exportable fruits and vegetables

Fztiit or |

vegetable |

Avocadon

vm ■ «

Banana

Esnaisa'•

L

Bell pepper

Bitter melon

Cucuzsbor

Variety

Seedling

Cavendishit

i

Cavendish

Degree o£

maturity

mogreen

ti ni

i

!

n n

n n

\—/ -v — ■*-£' . 1

" " I

■! 1CalifftVondes

n n

Donvar long '

Burpee hybrid

Round

Sollection

llOoi

4*45

67

3 ' i7

T

2

2

31

32

7

2425

2

12

11952date

!

1/302/20

1/302/203/20

I2/20

1/282/20

2/20

2/20

Locali-^r

1

Kilo

I'Saunowilin

Kanoohe

litinoohe ]

j

Eva 1

Kilo

Maui■ ■■

Ho.

2540

1657

130

160

150

(y->0

130

20

4941

S20

40

50

20

1 20

1 ^100

fe 1

Ibso

30

30

cO

1695

30

50

45

125

40

40

oO

56

3.1

1212

275

20

60

25

2?

267

rotal

0

0

0

117

0

0

0

0

0

0

0

0

0

0

0

270

1602

0

0

0

B739

0

0

0

t

Pes?

Ibo

0

0

0

♦C$9

0

0

0

0

0

0

0

0

0

0

01

0

0

0

1*559

0

0

0

1

$or.

1

0

so

0

0

0

23

0

0

0

■"7T

0

Emergen

cucJ

0

0

0

0

0

0

163

1123

0

1585

"I)

0

1/

!0«peP«O«

0

0

0

0

0

0

0

0

0

f)

"XT'

0

0

0

0

0

0

0

0

0

fl

0

0

0

other

0

0

0

0

0

0

0

0

0

0

0

Page 25: (Formerly Oriental Fruit Fly Investigations0

Table 8 cont'd

Fruit or

vegetable

Mango

Papayam

n

!T

Variety

Haden

Solon

n

Bspaya \ Solo■n »

! • •

Jfcpesa

PJjieapp3jD S/

n

it :

Solo

Smooth

Cayennen

n

Degree of

maturity

m*green

1/4. ripe1/2 ripe

eu green

n it

n u

n 11

l/4 ripen 11

. u

Collection

Noo

*■<•

4-

56

57

59

'■tO 1

39

1952

date

1/111/282/26

3/4-

1/111/2$2/26 j

| i [1/2 ripe

1/4-3/4 X"ipratoon •

plant crop

re/boon

plaat crop

30 • | 3/4

•0

i 75

76

77

73

A

TO

1

3/143/14

1/29

Localitar

Kaneoh©

Koalo&nQan n

Kaneohe

Kailua

Koaloa

Koaloa

H«.PoiTd»Ks

4207-StlOA frJ.tl*)Wci

4202

HoPc4207-

Fruit

13IS

13

40

23

2m4O

50

155

40

2540

21S9

40

£00

30

ipio

| 30s 50

40

150

Wt-

Iba-J

n

1511

3927

50

65

7374

332750

2SU

50

I9CV,

72

12S

103

195

larvsie

Total

119

94

00

1

0

:.i

i 04.0

0

40

• 0

1GCS

0

0

0

0

0

I " 1

pet'

1U55

7.932*55

0

0

°0

-010

^ ^. Emergence-'

Uors

77

71

1

153

0 J°" i

0 »

t

0

0

0-

-GGO31)I

0

x

oucl

0

0

0

0

0

0

0

0

0

no

00

0«p

0

0

0

0

0

0

0

0

0

6

0

0

OoOe

0

0

0

0

0

0

0

G

c

77

0

0

other

0

0

0

0

0 "10 ;

0

0

0

i

0 1

-i

0 10

cont*<3

Page 26: (Formerly Oriental Fruit Fly Investigations0

Table & coated

-203*

Degree of Collection

^s ;s QapQo poraulcatuaf Ooo»Oft ocmhiliiaPineapples shelled beforo placing in holcLLag boxes; all others held entire.

Page 27: (Formerly Oriental Fruit Fly Investigations0

-204-

V Ffeetors Affecting the Keeping Quality of Gut Flowers. Foliages. Ornamentals*Fruits,, and Vegetables in Relation to Quarantine Sterilization Requiremanta

for Ezport, (Ernest K. Akamine, H.A»E.SO5 '■

The following is a resume of the studies conducted by the Departaant

of Plant Physiology University of Hawaii Agricultural Experiment Station,

duping the period ending I&reh 31, 1952, on the tolerance of the various

commodities to treatments required for destroying tha oriental fruit f2y«

Certain treatssnt, storage, and personnel facilities of Grant Ho0 5X of the

Industrial Research Advisory Council, Territory of Hawaii, were employed inthese studies*

Banana (Chinese)

That the tolerance of Chineco banana to tho approved sthylone dibromide

fumigation varied with fruits from different locations was rsportsd in the

last quarterly report. Investigations ucjra continued during this quarter

to determine the causo of this diffs.;'ar.c3 in tolerance. iToaa of the

physiological faotorn takea into ccac:laera"iion ao xpx seems to be the deter

mining factor (tests 43, 44, 45, /*6, A8, 49. 50, 51, 52, 53, 54, 55). It\tzb, hor;3vor? discovared that ato.?:lnj tha c2i:r.iu; fo:-: about a iveok at 55° F«

before fumigating reduces the degroo of injiu-y (tests 1,7. 5S, 57) * Furthertests on this aspect vill be

Although the approved etbyleno dibi-oitd-do traainant itself doss not

influence the developmant cf storago rot of papaya, the loss of marketable

fruits due to this rot is quit© considerable especially with fruits from

wet areas and those harvested during the winter months. This rot is caused

by a fungus called anthracnose. Suiaerous tests -tfers conducted in efforts

to control this dissaso* Soakiag the fruit in hot water at a temperature

of about 110° F. for about 20 minutes before fuMgating uith ethylene

dibromide significantly reduced the rot incidence. Tho hot water treatment

has as such as doubled the number of sarliatable .fr;?.itsa This treatment also

cleans the surface of the fruits which are tisually eove-i-od'yith lates, dirt,spray residues, etc Det-sils of these tasts will be raported soon in

suitable publicationsc

Avocado

Euraarous variotiec of avecaden ere V-aiiig collsctaci fron throughov.t the

tei'ritory to tsot their degrso cf tolcv."n',:o to th?. approved cthyloaa dibs'osaide

treatreent and to detsrr.iino thoir Icocpiiig q-.j.alii-.;r c.^d eating qual5.ty (fatcontent, noisturo cont-snt. taste, arc-:n. sosi-;;dil:'lo portion "ratio, sisa,etc). Sams 50 naiced aacj scadliag vcric-ties hsvo alraacy been tested andit will bo sor.a tiss bofove ail vai*ietios will Ii&va boon bectsd. Thera is

considerable variation in the factovc studied ar-xng ths different varieties^

Those rating high in tolerance to. the fumigation trsatcsat, keeping quality,

and eating quality will be recommended for consiiarcial plantings for the osposi;tradeo

Page 28: (Formerly Oriental Fruit Fly Investigations0

~205~

Shipment Report (X«R,A8C. Project 3$ RM. 95-2)

The test shipments were planned by Kenneth I. Hanson and Dr. FarcyPhilipp of Project 3a, Expansion of I-feinlend Markets for Hawaiian WinterVegetables, Fruitc, Flowers, and Foliage, in cooperation u«ith Dr. KobsShoji and Dro Toru Arlsurai of Project ftc, Storage Life, Quality andMarketability of Fruits* Vegetables, Flowers and Foliage for ExportMarkets* Jack Ishida assisted in preparing the shipments* Kr» Hansonacoozapanied shipc©nt Uo» 9 to los Angeles, Shipaoata TSo0 10 to 15 weresent to coast markets by Dr0 Philipp and lOr,, Kobe Shoji, received andinspected by Mr.. Hanson*

tto. 10 To Seattle? Hawaiiaa Plenter, November 3, 1951,

Papayas, pineapple, avocados, bananas, string beans, andcueiaabarso

Kbo 11 To San Francisco, Hawaiian ■Wholesaler, November 23, 1951OPapayas, avocados, cucumbers, string beans.

Ho* 12 To Los Angeles, Hawaiian Packerj, Ibveiaber 29, 1951,,Pineappl© container testo

Ifo. 13 To Seattle, Hawaiian Planter, December 10, 1951*

Papayas, pineapple, avocados, cueraabsrs. and string beans.

®o» 14 To Los Angeles, Hawaiian Refiner, Dscembcr 20, 1951*Papayas, pineapple, cucuabarG, o.ncl string beans*

K6» 15 To San Frsncioco, H?.-.-a:L:laii Pilot, Jcsuary 97 1952.

Papayas, bananas, ciicuiaberE, sn£ otring boans*

I&rkot research raid trade sciucuiion vorl: win cai-ried on in the idiolo-sale and retail stores in Los .i>i£c7.e3, San Frascicco* Seattle, and Poz'fclando

ssmms

PAPAYAS

Six test shipments wore eent to coast serkets to study the dsmLopmantof decay en route and the coloring and ripening of papayas from differentshipping districts*, Upon arrival at the coast markets, the papayas were

taken to wholesale produce warehouses for inspection,, or to the UeS»D0A,Terminal Market inspection office. Preliminary inspection reports for eachshipment were sent to the Agricultural Economics Dspartasnt of theAgricultural Sktension Service and to the project leader of 'XeTUKG* Project

ShiptBant Fog 9t Twslvo car-tons of papayas vrsre treated and packed inHonolulu on October 22 and shipped at 55® F, to los i&gelos. This lot uasheld at 60° to 65° F6 after arrival :lu Los Angslos, Inspoctiona t;ere asda

^^ on October 30 and irovsmber 2^. 0a October 30, 8 days from paaking,. 21 perf cent of tho fruit showed stcn>eKd decajr. Tvo days later t?ais had increased

to 43 par cent*

Page 29: (Formerly Oriental Fruit Fly Investigations0

Shipment Nqo 10t Twenty-four cartons were treated and shipped toSeattle as follows!

(a) 12 ctns. of Oahu papaya from the University of Hawaii onNovember A» ahippad at 47° P.

(b) 12 ctns. from a commercial shipper in Hilo, Hauaii^ on Kovember1, shipped at 47° F«>

Tfoon arrival the fruit uas kept at room temperatures of 46° to 50° Fofor two days. Four boxes war© t&en hold at 70° F, for two daya0

(a) The Oahu papayas showed 6 per cont surface decay on arrival*The decay was apparently caused hj box braises and piekiag bpuiseso

Twenty-two per cent showed stem-end dscsy« Three days later the stem-enddecay had increased to 30 per csntc Coloriug appeared to be normal,, All

fruit packed half-ripe was ready to sail on arrival, Nearly all the one-

fourth ripes had changed to half ripos, and nearly all mature greeas had

changed to one-fourth and half i

(b) The papayas shipped from Hilo came from two locations oa Hawaiiand were designated as lot M (^sesnoto) and lot T (Tanioka)*

Neither lot showed any anthracnose or other surface daeay on arrival,

but both showed heavy etea-ond decay., lot M had 30 per cent stem-end

decay which increased to 50 per cent in two days0 Lot T showed 38 p©r cont

stem-end decay T&ieh increased to 45 psr cent in two days.

In both Hilo lots the coloring was norml, all fruit arriving at ono-fourth to one-half ripa. This fruit is finsor, has a brighter color thanthe Oehu fruit, and ripens more slowly.

li Eight cartons of Oahu pnpsysa trsre trsatod Hov©aiber23 and shipped at 4.7° P. on tho Baualicji viholoaaler to San Fi^anci3co. The

color and quality of this shipment rero good vith fey .fcruicad fruit and no

anthracaose on the surface. The fruit uao liold at rcora csTnpsraturos of

60° to 62° F« on arrival,, Twenty-threo cut of 56 iiapajas, or 50 psr centflshowed stem-end docsy upoa arrival. However, only 9 por cent had enough

decay around the stem to make the fruit unsalable the day after arrivalo

Shipment ITqp Igt I^ina cartons of Oahu papayas were treated on December

10 and shipped at 4.7° F» on the Hawaiian Planter to Seattle. The fruit nasplaced at room temperature of 4-5° to 50° F» oa arrival. The fruit trasfirm, with a few scars and bruises on i&© surface,, On arrival, 4 per eenthad surface decay and 5 per cent showed stsra-end decay,, Thi3 lot was

salable on arrival, but the cold weather ratorded coloring. Some of thofruit was placed in retail stores vith instructions to keep it at 70° F«,or above if possiblee

lo«,,_1A: Ten cartons of Oahu papayas vsro treatod. oa Decomberth Hi

, o u ppays s reatod oa Decomber

16 and shipped on tho Hawaiian Refiner to Lojj togeles at 47° F. The qualitywas goodj the fruit hard and fina on arrival, Sii: cartons t/ore packed

ir/ith nature grson fruit, ons x.rilth orie-fourth rips fruit, and threo withons-half rips fruit, 'i'ho fruit shoved a alight change of color in transit.

Page 30: (Formerly Oriental Fruit Fly Investigations0

-207-

Upon arrival the fruit was held at room tesgjeratures of 52° to %° P.There was no anthracnose evident on the surfaces 11 papayas, or 14 per cant,showed varying degrees of stem-sad decay which increased as the fruit washeld in storage*,

gMcmsnt Ho« ?fo Fifteen cartons of Oahu papayas, treated and packedon January 9 in Honoltiluj, vbsq shipped at 42° Po oa the Hawaiian Pilotarriving in San Francisco on January U* The qusality was good, fruithard and fi2% with wry little decay on the sm?face«, All eartons werepacked with nature greaa fruito

The temperature on the pie:? -jag 50° to 55° P« upon arrival, and on thehouse floor the temperature ma approximately 52° to 58° P. The

papayas wore left on the pier for one night, then taken to a warehouseoffice for inspection.

On January 15, aftor six days or. fcae ship at 47° F,, 79 per cent verenature grson and 21 per cant uere onc-fcurth r£poe

On January 16, sevan cartons of tho lot vero placed in a ripeningroom at 67° to 73° F6 (humidity 70$. Sis caVlcns wore hsld on the warehouse floor at a tempsrature of 50° to 55° P.

Results of observations Eade on riponing are shoii?n in table 1.

Table 1*—-Comparative Ripening of Papayaa During 7 Days in Storagein Warehouse and in Heated Room-*.

Stage of

Mature green

2/4 ripa3/2 ripe

Ripe

January 16

house

heated

room

Per

78

22

0

0

80

20

0

0

After 3 daya

hous©

heatedroost

5640

4.0

2668

3

3

After 7 days

house

heated

room

Per

4650

40

1

31236

s Temperature in Tfflrehouse yas between 50n ar.d 55° P« and in the heatedroom bstween 67° and 73° P.

tt* Percentage of each lot*

The fruit in the heated room colorsd raich aos-a rawidly than the fruiton the tmrehouse floor but dovslopod noro stssi-and decay.

The fruit held at 73° P. showed 13 per coat etac-end decay aftor threedays? the fruit held at 55° P. ahouad 2 per cent stei>aad decoy, • At the©nd of 7 days, the heated fruit shouccl 41 per cent decay and the fruit heldon tho warehouse floor showed 28 por cent decay. HbHOver, some of the fruitshowing stem-end decay visa salable during the first fotf days.

Page 31: (Formerly Oriental Fruit Fly Investigations0

-20&-

Papayas from different islands vary in quality, color, texture, and

flavor* Some papayas ere ready to eat tiheh half-ripe; sosa are not ready

when almost thr6e»fourth sip©. Th© amount of anthracnose decay and stei>»end decay varies in fruit from differaat islands and from different loca

tions on the sama island.

Stom°end decay in fruit reduces the shelf life and limits sales*

Although the decay is present around the stem and increases daily after

it first starts, the fruit is often salabla upon arrive!* However, the

presence of tho decay often necessS-tates repaclcing which adds to the sell

ing expense,, Inspaction of some vapor heat treated cammereS.al shipments

shoved no stem-end decay upon arrival or 3 days aftsr' arrival.

Some gresn fruit is arriving on the rcarkotc This fruit often fails

to color bafora it develops both anthracaoeo and stoE-enci decay. All fruit

should have a definito color brerak or be one-fourth to one-half colored

at the time of shipping* The low taraparaturoo in eoaat cities in the

winter and spring months retard the coloring of papayas on the retail

stands. Few stores have facilities to heat the fruit to 70° to 75° Fe ia

order to induce coloring* Green papayas a:ra seen in oil stores on tho

Coast during this periods The low teznperaturQS havo a ssrious effect on

the proper coloring and handling of the fruit •>

Two largo shipments of pinaapplo wera made to I«os Angeles to study ths

amount of bruising caused hy tha various types of shipping containers now

in usa» Ths Katson navigation Coiapaay and a- contaisier corporation cooperated

with the shipper is thass experiments. Observation of the fruit lias aiado

on coast mrkets isj th9 writer.

Shipment No, 1: Pineapples wore packed in five differont sizes and

types of containers and wera ahippsd on the Hatiaiian Packer to Ion AngeleB

on Uovsiaber 28O tfpon arrival in las Angeles$ the fruit waa placed ia a

wholesalar's warshxyus® for isaspection. Ths quality of ths pineapple in ths

entire lot was only fair and sons of the pineapples wore vary ripe,

Ths following table shows tho amount of bruised :?ruit in each type of

containers. Only container and shippirsg baniiB©3 *>rars recorded. In sesrainstances it was difficult to determine father tlia fruit had besa bruised

before packing or-bruising was caujsed by ths container..

Table S.-^-Percentage Bruising of Finscipplos v>j Typo of Shipping

Container. Shipaant Ho. 1.

Quantity and type of contains!

10 wirebound crates

10 old style wooden boxes with heavy slats

10 snail corrugated light cartons

(canned pineapple carton)10 large corxugatsd 'light* ca'rtoas

(canned pineapple carton)10 large~sise corrugated car"fcons

(asphalt between layers)

<er of" Percentage

braising

587

36

50

120

20c7

28o0

12,0

2«5

Page 32: (Formerly Oriental Fruit Fly Investigations0

-209-

Shiprasnt Hqo 2> Pineapples wara shipped to Los ibgales on the I&waiiaaRefines? on December 20 in twenty containers using four different types andsiaea, The quality of fruit in this shipment was batter than in the firstshipment* The aaiount of bruising appeared to ba a little less than thefirst shipffisnt. The same procedure of inspection was followed as in ship-mant Ho. 1.

Tabl© 3»—Percentage Bruising of Pineapples by Type of ShippingContainers. Shipment Noe 2.

Quantity and Typa of Container

5 heavy wooden crates with slats0

5 irirebcund crates

5 light corrugated cartons with excelsior

5 corrugated baaana cartons (long)

Slumber of

pineapples

40

30

22

50

Percentage

bruising

17.520c 0

14*0

4°0

Two crates with fruit packed Breaming trp showed 8 per cent bruising,.

Three crates with fruit packed on sidas showed 25 per cent bruising*

Remarks

I* The bruising of pineapples ehippsd in heavy wocdan containers witfa-

out corrugated wraps Has due mostly to the praser-re of ths side slats

against the fruit* This was particularly noticeable whoa the pineapple was

packed too tightly in the box, See® of the fruit had been cut by the slats$

others wore bruised froa the -weight of the boxes vhsn piled one on top of

another^

t '.jas packed in corrugated cloo^er: ez.0. a padding of excel

ho bos, bruisiuf; vp.o light. Bruiaiiis appscrod to be mach

ugated wraps

2c When fruit

sior Msaa used in t ,

worsa vdthout corrugated wraps

3«- A heavy eorrusated bo?: holaiag 12 pinoapplss and used bj a coiai!»r-

cial shipper proved to ba an excellent container. A cc:o:cuga-bed box \rxbh

asphaltusa between laj'crsj holding oij: pineapples fin oections, has also

proved satisfactory*

BMAITAS

Shipmsnt 3?o» 10. to SaattlQAiiaavraixan Planter.,,fiNpvsigber...a, 'Twocrates of Caveadish (Chinsser^naBas^roKi Jfenoa Valley* Oahu, were packedin hands in tJirebomsd containers with shredded paper and shipped at 55° F»

after treatment with ethyleas dibromide. Quality was exesllent on arrival,,

However, color was a little too light and the fruit was too ripe for com

mercial shipsents to sail trail in the Seattle market.

Shj-pmsnt No. 15 to San Fgancj.ocoT Hawaiian Pfvlot, Ten

bunches of bananas from Manoa Valley were packed in a large crate with ex

celsior and bananas in hands were paclsod in firs corrugated cartons after

treatment with ethyleao dibrord.de. Thoj usro shipped at 55° ?• Upon

arrival in San Francisco, tho bananas in the largo crate wars unpacked and

taken to the levy Zentnar warehouse where they usro placed in th© ripening

room at 60° F. The'bananas in hands vsro taken to "Mi© 0. Lippifor ripening.

Page 33: (Formerly Oriental Fruit Fly Investigations0

~210~

(^ ' The quality of bananas in bunches and in hands was good. Some werebreaking color on arrival; a few were greeno Comments on quality bywholesalers and others were as follows:

"Arrived a little too yellow and should be picked slightly greener*n"Ship slightly greener for best results."

"The skin of the Cavendish banana is very tander and more susceptibleto bruising than the Gros Michel bananas,n

"Bruising not excessive in the shipment*n

nDo not use excelsior as it cuts into the skin of the banana and leavesimprints.H

"Too mich shredded paper in cartons. Air is cut off around the banana,a

Remarks ■ . •

Market reaction" was good and soisa interest was, shoiai by thos9 dealerswho had sold Hawaiian bananas jsa?3y yeava ago* Sams of this shipiaont ofbananas wa3. <3oliv©r3d to retailors who vore familiar with' Eawaiian bananasand had sold them icany yeai*3 ago.

Wholesalers want to build up the old teada slowly sad recoimaencl. small

revived if quality is good, "They rocounjancl shipping Hawaiian bananas in

stems in the green stage with no yellow color break and placing them inripening rooms on awival. This is the irsthod ussd in handling bananasfrom Central Ansrica. They suggest that further experiments be saade with 'shipping temperatures and. methods of handling in transit, etc, CentmLAmerican bananas are shipped at temperatures of 53° to 55° ?„

Dealers also stated that thi3 variety is difficult to sell in eompeti-

t5.on with the Gros Michel variety frca Contrd -tecrica but felt that at ■first ssall quantities could be sold if the quality v&e good*

AVOCADOS

Ttjo tasii shipiosnts of avocados wara sent to Seattle and one to San

Francisco,, Irag boxss usrs ussd in wo of the shlpjaants ?-^ flats in anotiier0

^^^^^S^S^^^^^SJ[^^^^JSSr. *~-3 avocados voro toosoft aad over.vips, and vrero unsalable on arrival. Ths flesh was discolored

and there i:ao a ^-jatery appearance ai'ouad -the seed. Ssvsral &vocado3 should

decay as well as a breakdown of the fleali. S5.n-3c \ioso xog la

lJo«.-,3.1A,Jlo,ISan.Franoiccpj...^ovsy^gr..g3j.^l951t TIiq arrival

condition of avocados i/as escallcnt, Ono xiholcgalGr irac eatisfiod with thequality but stated the sise was too large, Torjnirial warlcet inspactor-s

report: BGenarally fins to hard, no decay. Flavor very good on turning ■

or rips; fruit markstable,n

Two fcosas (24- stroeados)lfrom Grower Ho. 630 in Kona, Hawaii» Outside appsarance good on arrivalj

9 fruits soft, 15 fina,. Eight avocados out of 24 were cut opaa* All showed

broan .discoloration of the flosh and i-satery appearance around idie seed.

Page 34: (Formerly Oriental Fruit Fly Investigations0

/ps. Four boxes U6 avocados) from Grower Ho. 1505 ia Koaa, Arrival condi«V " fcion was goodj fruit was green and fix©. Color and texture of flesh u©r©

goodj no decay or browning around seed; slight pitting on surface,. Flavorwas excellent! seed large, One retail store reported norssal ripaniag iathe store.

Remarks

Wholesalers want small avocados—24 to 36 to a flat of 13 pounds. Goodgrade, large sise avocados will sell hut at a lower price than snail avo~eadoa. Results of inspactioa of this test shipment indieata that keepingquality varies a great deal with different vaz'ieties of avocados.

CUCUMBERS

Five shipraants of cueisabars were Eade to Saa I^ancisco, Los Aagalss,and Seattloe The cucumbers -^re treated xrith ethylene dibromide for.2 hoursat 70° Fo and shipped at 55° £> Iospections were md© on arriiral, and thecucumbers shown to uholasalsrs to determine market reaction*

Shipment No, 10? to Seattle^ November. 4. 1<?$1 (2 boxes)% 70% darkgreeni 20$ definite yellow colors Mf°light yellow color; 12% showedshrivelling; sise good, flavor good.

p Ko, 11. to SanjP^M^^^Jia^^^^^Jc^i^ (1 box) s Arrived

in bad condition, UiffiBrke.table on account of^decay and ysllou* color*

fairo Only 18 cucumbers out of Jt2 isirkctr/cis "or. arrivnij iTslioued shrivol-ling and 14 .showed decay on the surface* Scars ovsrHatui'3 when" piclcedo

^S^L&tjy^^a-^LiSgS^SjJJscCTflterJi^ (I.ijok): lfi% bslour gradeduo to shrivelling, or decay on sui*face, ' 4J# iiere grssn—quality of thosewas Ht

Sbipnant go. 15. to San Ifeancisgo.:.. Jaj^^vsr.ig^Jg.'H (2 bcaces): Excellentquality, color greone Hard and fin>~i% decay, no nhr:WelliEg or pelting*Equal to Florida and Cuban cueianbsrs*

Remarkg

Cucumbers in all snipaants except ono showed yellouing said sos® doeayeWhen cueuabsrs are picked overmatura and shipped with a slight yellow color,arrival condition was poor, Dark green cucumbers, properly graded on paek«ingp carried well and arrived in good condition* Hholesalors showed interestand made inquiries wfcaasver medium aiscd. dark green eucumbGro ware displayedon the serkete QuajJ.ty siast ba equal to nsinTumd quali*^"*

STRING .BEAMS

ThsIvo lug bosee of string besmn, all of tha .LiialraLoi variety, wer©sent to coast !?arkatfo TI7.0 beans -.rare tveatod with otl^rlens dibroni5.de for2 hours at 70° F. and shipped at 55° 2?. On arrival they were inspected endnhov.ii to intorosted vltole-SElero .vi S&u Fr<2«.ciacc, los Av.Qolcn,, Seattle, andPortlande

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ft No«. 10,rto Seattle, ITpvembar ^ (2 lug boxes): Color fair,too light "on arrivalj™67# good, marketable stock* 15$ brol:en$ $shrivelled and too amallj 3% decay and

Shipment Ho, 11, to San .Francisco, November 24 (2 besres): TorndnnlMarket inspectors' report: "Contents show beans generally rus3etted~»

ranging from few spotG to practically entire bsan affectodo Quality sari

eonditioa aside from russettiag good."

broken aad feu ssall and inraaturs* This lot not Earketable dus to

russettingo

Shimsant Ho, 13, to Seattle., DecemberJQ. (2 besea): Very light incolor; 85% Grade A. i5% iaanatura and^^blendshss axA broken0

gbiyjamit. No^jy..jap Loa Aogelss, Daceaiber.l? (3 Ivg bo^es): In goodrfresh, crisp condition. Idght colors 8Tj shrivollcd aad too young? /$broken and 3% decay and raoldf and er.tixig quality gocdo

Shipment IIoi,i_.l?,titp San Francisco.,. January.. 10 (3 lug boxes): Qualityfair,, Ifeny too young, shrivelled; boanc sppsarod frocks soeb interest

shown by tradstt Placsd in four "wholesale stores*

Remarks

Good string baa-is of tho L'jsluslei varisty v.:dJ. arrive' in salaM.e

condition on the Coast after trer.t;is:it '.-i'-h cthylorio f2:i.bi'ovd.dc. I7o*«£ver,

they should be picked at tho right .Ttago cf bvbv^ity astf shciucl not bs tooyoungo They a'iould to. graded for quality'and packed in en L.A* lug boot orpnwan wirebound contsir.sr without \ic:r. p:iv.er or c. liaar* A chipping tem

perature of 55° Fo appoars to be s2.t:«.3.Ctictcry.

HIBISCPS

On September 27, 1951f c .arantine regulations on the shipjaent of

hibiscus plants and cuttings to the IJaisland Tiera modified to allow entry

to the MainlsiBi after ii

On October 22, 1951, a teat shipment of 13 hibiscus plants obtainedfrom the University of Hawaii aad a HonolvJLu grouor 'c^.s csnt to tho Mainlandafter fumigatioii irlth insthyl broiaids by the Beard of Agriculturo and

and three woro iassced on ths ship b3.Ccu*a arrival. Of the plants chippedat 55° Fo, four had roots psetsd in aoas l:afcro sl'dpssnt and thrcs vera leftin the original can for 6 days am aoEcad en the chip brafors arrival*

All plants survived accept onct Ti-ic c:\3 p".r.-vc bed r:r.rdly amy rootsystem and iras psckc/! :l?.i rc-oss on clav c:? nh:li5r.5ivV. enr; i.^lc. .c.t a ts:it?::rat'jrs

of 70° to 75° F* on tho shipt-

Tho loaves of the plcurbs packed :& koos and carried at 70° to 75° F,appeared moro wiltec.' then the loaves o." plantc in crisir^l coiitaincrs. Tfcogeneral appearanco cf t!ie foliage of plr-ita crj?::lzd r.t y3° ?«. «'ca better

than that of plants carsisd at TCP to" 75° F«

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-213-

/FN Hibiscus plants can be transported to the Mainland with roots packed^ in moss at a temperature of 55° F» .or at 70° to 75° F8 Proper handling

from ship -to destination is important as plants dry out at high teimpsra-tures aid low huaidity«

MARKET RESEARCH AHD TRADE EDUCATIONAL HORK

lo Galls were made on brokers, x&olesalers, and retailers tfho handleboth fresh and processed Hawaiian products in Los Angeles, San Francisco,

. Portland, and Seattle There was a genuine interest shown &n Hawaiian

goods by many of the companies visited, Thoy ware interested in knowinghow papayas grew, hc-v large the industry was, whether It was seasonal ornot, and what the future ohipsant might be* Photographs were displayed andinfoEffiation on Island fruit crops war? given« Calls were imide on retailersand wholesalers with brokers' saleenssn ia Sea Francisco and much usefulinformation was exchanged on the possibilities of selling local products,,These salesican hsys long established conasctions with mary cstores and soaseof the buyers in the stores ara inclined to learn all they can about newproducts such as the papaya, both fresh and processed, and Hawaiian jamsand fellies* Stores in the jnora prosperous residential districts ar©interested, as mnp of their customars have been to Hawaii and ask for

the products* The prico of both fresh aad processad products varies agreat deal*

2, A short 5-sninute saoving picture film of "Khe papaya growing industrywas aade in the fall of 1951 and edited by the secretary of the Los Angeles

r^ 'Hholesale Produce and Brokers Association., !Fhis film Is now being usedweekly in classes organised by the association for r9tail clerks and producenan in Los Angeles and vicini-'^r* Mro Bill Bawloy of tho association givesthe course and states that there is an interest shown In papayas at

3« Literature on papayas v&a distributod to aa*ey retail produceclerks and store opare.toro. Inforiration on vitamin r.nrl other food ualueof the papaya xras gi'j3:z. to nouopapisrs, r&altli 3tore,'.i and Juice stands.Publicity was given in the food adv-ortininj:-: sections of nGv.-sKapars and thowrites? appsaraci on on© telewis.ion vh&x.

4. Window displays of IcrlDutl food products v-3.v-3 sat up in Ssattle,Portland, San Francisco, and Los Angola:? is coopbi-atior. ^;ith the ?-TatsonNavigation CosEiany. The displays crdatea scaa latersst v&d jnany inquirieswere received about Island products a?d where- they could be obtaijaed.

This work will be continued and enlarged* '

A display of Island products was seat to Bsar Creak Orchards throughthe cooperation of *&■* Wood of Hawaiian Airlines e.nd the United Airlinesand shown to the president and some of the staff moEbars0 Shis coispanyspecialises in gift jjackages all tho year round and covers cany cities inthe United Statoso The eompa:ny is interested in three products«"coco.(iutbutter or topping, poha Jam, and frssh pineapple and would be interestedin contacting'shippers in Hawaii,

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ACKHOtJLEDGMEHES

The writer wishes to acknowledge the aid and cooperation of those

who assisted in the test shipment work in Hawaii and on the Coast*

Dra, Kobe Shoji and Toru Arisum of Project 5k, Dr, Perry Philipp,

aeting project leader of Project 3a, and Jack Iahida of the Agricultural

Extension Service, procured, and shipped tho fruits and vagstables after

treatment while the writer was on the Mainland frcxa October 22 to January

2/{«. Matson navigation Company personnel in Hor.olulK.—4vlessrs« I»o Lo

Westling and Prod Zirni-'-arranged for storage facilities on tho chip*

1-Satson Navigatiou Company officials in Sen Francisco. Los Angeles,

Portland, and Seattle, iracie arrangemonbo fo:c receiving the eliipnsntSo ■

Mr,, Sugone Tarr in San Francisco and Ift\, ."Mt'ard Rcalfo ia Los Angeles

helpsd ±a rcany

Thanks are also duo to llessr.^ Say 0. Liudatrora and Sam Iiagusi,

Torainal I'iark9t inspocoors of ths ?rccucti«5a and I-Sirlcetyjag Administration,

Uo S«, Depai-tmant of Agriculture, for fun::!s!i5.ng spaes ani inspacting •&&

vegetable shipnjsntst, Jlr, C« A, Rogers of Cala\To, Inc.- in San Praneisco

and the Consolidated Produce Gonpar-j iii Los Augeles avranged for storing

and handling e2psriK2ntal shipmaats aad assisted in inspecting and report«»

ing on arrival condition of fruits* The Levy Zentner Gos^jany, 0o Idppl,

Jacobs l%lcolai and Burtt, aud L, J« Hopkins Company alao gave ijarehous©

space and assisted with the ercpsrimsntsl shipments* The Olympic tTarehouse

Company and Calavo, Inc,, officials in Seattle assisted with the test

in that cityo

(signed) Kennstli I. Ifeason