forensic dna past, present, future kathy sullivan illinois state police joliet forensic science...
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![Page 1: Forensic DNA Past, Present, Future Kathy Sullivan Illinois State Police Joliet Forensic Science Laboratory](https://reader036.vdocuments.site/reader036/viewer/2022081504/56649d595503460f94a38daa/html5/thumbnails/1.jpg)
Forensic DNA Past, Present,
Future
Kathy Sullivan
Illinois State Police
Joliet Forensic Science Laboratory
![Page 2: Forensic DNA Past, Present, Future Kathy Sullivan Illinois State Police Joliet Forensic Science Laboratory](https://reader036.vdocuments.site/reader036/viewer/2022081504/56649d595503460f94a38daa/html5/thumbnails/2.jpg)
Case Study: Jeffrey Knight
• Homicide from Kankakee County in December, 1997
• A head, hands and feet were found in a garbage bag in the woods
• Jeffrey Knight was suspected of killing his step mother, Marguerite, and disposing of her partially dismembered body
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Case Study: Jeffrey Knight
• Screening results: many blood stains found, but only some were human
• DNA results: performed using DQ-alpha/Polymarker and D1S80
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Case Study: Jeffrey Knight
• Stains found around the barn, on a reciprocating saw and the trunk and floorboard of a car match Marguerite Knight
• Random match probability is 2.1 billion Blacks, 5.4 million Hispanics and 5.3 million Whites
• Jeffrey Knight was convicted and sentenced to 60 years in prison (he is eligible for parole December 2027)
![Page 5: Forensic DNA Past, Present, Future Kathy Sullivan Illinois State Police Joliet Forensic Science Laboratory](https://reader036.vdocuments.site/reader036/viewer/2022081504/56649d595503460f94a38daa/html5/thumbnails/5.jpg)
Case Study: Timothy Evans
• Summer of 2002
A series of night-time home invasions and sexual assaults begins in Joliet and Bolingbrook
• Media names the assailant the “Nightcrawler”
![Page 6: Forensic DNA Past, Present, Future Kathy Sullivan Illinois State Police Joliet Forensic Science Laboratory](https://reader036.vdocuments.site/reader036/viewer/2022081504/56649d595503460f94a38daa/html5/thumbnails/6.jpg)
Case Study: Timothy Evans
• Evidence from one of the “Nightcrawler” sexual assaults is received by the lab in May 2003
• Underpants of the young victim are submitted to be tested for biological fluids (semen and saliva)
![Page 7: Forensic DNA Past, Present, Future Kathy Sullivan Illinois State Police Joliet Forensic Science Laboratory](https://reader036.vdocuments.site/reader036/viewer/2022081504/56649d595503460f94a38daa/html5/thumbnails/7.jpg)
Case Study: Timothy Evans
• Testing indictes saliva on the crotch lining and the stain is removed for DNA analysis
![Page 8: Forensic DNA Past, Present, Future Kathy Sullivan Illinois State Police Joliet Forensic Science Laboratory](https://reader036.vdocuments.site/reader036/viewer/2022081504/56649d595503460f94a38daa/html5/thumbnails/8.jpg)
Case Study: Timothy Evans
• DNA analysis yields a mixture of profiles consistent with the victim and one additional male contributor
![Page 9: Forensic DNA Past, Present, Future Kathy Sullivan Illinois State Police Joliet Forensic Science Laboratory](https://reader036.vdocuments.site/reader036/viewer/2022081504/56649d595503460f94a38daa/html5/thumbnails/9.jpg)
Case Study: Timothy Evans
• Minor male DNA profile is entered into the DNA Index
• June 11, 2003 a match is reported to a sample from an Aurora case two years before
• The matching sample is a standard from Timothy Evans
![Page 10: Forensic DNA Past, Present, Future Kathy Sullivan Illinois State Police Joliet Forensic Science Laboratory](https://reader036.vdocuments.site/reader036/viewer/2022081504/56649d595503460f94a38daa/html5/thumbnails/10.jpg)
Case Study: Timothy Evans
• Joliet Police Department is notified and on June 14th the suspect is arrested
• Confirmatory analysis verifies the match
• Random match probability of 1 in 20 billion Blacks, 1 in 310 billion Hispanics and 1 in 330 billion Whites
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Case Study: Timothy Evans
15,17 15,17 19,21
X,Y 14,15 29,30 15,16
11,13 12,12 9,11
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Case Study: Timothy Evans
X,Y
15,17 9,11
6,7 9,9 9,10
9,11
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Case Study: Timothy Evans
• Timothy Evans pleaded guilty to the charges against him and was sentenced to 50 years in prison
• The DNA evidence and CODIS hit were instrumental in providing law enforcement with the link to the assailant
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Advances in DNA Technologies
Yield Gel =how much DNA?
QuantiBlot =how much is human?
![Page 15: Forensic DNA Past, Present, Future Kathy Sullivan Illinois State Police Joliet Forensic Science Laboratory](https://reader036.vdocuments.site/reader036/viewer/2022081504/56649d595503460f94a38daa/html5/thumbnails/15.jpg)
Advances in DNA Technologies
Quantification Procedure
Tells how much human DNA
AND how much male
DNA is in the sample
![Page 16: Forensic DNA Past, Present, Future Kathy Sullivan Illinois State Police Joliet Forensic Science Laboratory](https://reader036.vdocuments.site/reader036/viewer/2022081504/56649d595503460f94a38daa/html5/thumbnails/16.jpg)
Advances in DNA Technologies
• RFLP
![Page 17: Forensic DNA Past, Present, Future Kathy Sullivan Illinois State Police Joliet Forensic Science Laboratory](https://reader036.vdocuments.site/reader036/viewer/2022081504/56649d595503460f94a38daa/html5/thumbnails/17.jpg)
Advances in DNA Technologies
• RFLP
• PCR:– D1S80
![Page 18: Forensic DNA Past, Present, Future Kathy Sullivan Illinois State Police Joliet Forensic Science Laboratory](https://reader036.vdocuments.site/reader036/viewer/2022081504/56649d595503460f94a38daa/html5/thumbnails/18.jpg)
Advances in DNA Technologies
• RFLP
• PCR:– D1S80– DQ-alpha– Polymarker
![Page 19: Forensic DNA Past, Present, Future Kathy Sullivan Illinois State Police Joliet Forensic Science Laboratory](https://reader036.vdocuments.site/reader036/viewer/2022081504/56649d595503460f94a38daa/html5/thumbnails/19.jpg)
Advances in DNA Technologies
• RFLP
• PCR:– D1S80– DQ-alpha– Polymarker– STRs
![Page 20: Forensic DNA Past, Present, Future Kathy Sullivan Illinois State Police Joliet Forensic Science Laboratory](https://reader036.vdocuments.site/reader036/viewer/2022081504/56649d595503460f94a38daa/html5/thumbnails/20.jpg)
Advances in DNA Technologies
• RFLP
• PCR:– D1S80– DQ-alpha– Polymarker– STRs– Y-STRs
![Page 21: Forensic DNA Past, Present, Future Kathy Sullivan Illinois State Police Joliet Forensic Science Laboratory](https://reader036.vdocuments.site/reader036/viewer/2022081504/56649d595503460f94a38daa/html5/thumbnails/21.jpg)
Additional Technologies
• Mitochondrial DNA
![Page 22: Forensic DNA Past, Present, Future Kathy Sullivan Illinois State Police Joliet Forensic Science Laboratory](https://reader036.vdocuments.site/reader036/viewer/2022081504/56649d595503460f94a38daa/html5/thumbnails/22.jpg)
On the Horizon
• Larger STR multiplexes
![Page 23: Forensic DNA Past, Present, Future Kathy Sullivan Illinois State Police Joliet Forensic Science Laboratory](https://reader036.vdocuments.site/reader036/viewer/2022081504/56649d595503460f94a38daa/html5/thumbnails/23.jpg)
On the Horizon
• Larger STR multiplexes
• Automation – fluid handling systems
![Page 24: Forensic DNA Past, Present, Future Kathy Sullivan Illinois State Police Joliet Forensic Science Laboratory](https://reader036.vdocuments.site/reader036/viewer/2022081504/56649d595503460f94a38daa/html5/thumbnails/24.jpg)
On the Horizon
• Larger STR multiplexes
• Automation – fluid handling systems
• High-throughput detection systems
![Page 25: Forensic DNA Past, Present, Future Kathy Sullivan Illinois State Police Joliet Forensic Science Laboratory](https://reader036.vdocuments.site/reader036/viewer/2022081504/56649d595503460f94a38daa/html5/thumbnails/25.jpg)
On the Horizon
• Larger STR multiplexes
• Automation – fluid handling systems
• High-throughput detection systems
• Accreditation
![Page 26: Forensic DNA Past, Present, Future Kathy Sullivan Illinois State Police Joliet Forensic Science Laboratory](https://reader036.vdocuments.site/reader036/viewer/2022081504/56649d595503460f94a38daa/html5/thumbnails/26.jpg)
Forensic DNA Past, Present, Future
• Kathy SullivanJoliet Forensic Science Lab515 E. Woodruff RoadJoliet, Illinois 60432815-740-3543