Innovation with IntegrityMALDI-TOF

Food and Beverage Microbiology

MALDI Biotyper®

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Geotrichum Fusobacterium Lodderomyces Anaerococcus Colletotrichum Edwardsiella Comamonas Pigmentiphaga Moellerella Nesterenkonia Listonella Udeniomyces Erysipelothrix Escherichia Streptomyces Bartonella Hafnia Terrimonas Pseudoxanthomonas Corynebacterium Streptococcus Aerococcus Saccharothrix Facklamia Schizosaccharomyces Tetragenococcus Lechevalieria Clavibacter Shimwellia Brachybacterium Leclercia Providencia Trabulsiella Xanthobacter Emericella Gardnerella Sporothrix Leuconostoc Pseudoclavibacter Alkalibacillus Debaryomyces Turicella Roseomonas Ruminococcus Scedosporium Dysgonomonas Staphylococcus Peptostreptococcus Paenibacillus Balneatrix Solibacillus Prototheca Cupriavidus Geobacillus Aspergillus Arthrobacter Mesorhizobium Acholeplasma Filobasidium Propioniferax Azohydromonas Chromobacterium Curtobacterium Kloeckera Austwickia Hyphomicrobium Cryptococcus Rummeliibacillus Budvicia Aquincola Enterobacter Sporobolomyces Brevundimonas Capnocytophaga Tatlockia Neisseria Salinivibrio Pullulanibacillus Arcanobacterium Tissierella Eggerthella Methanomonas Mucor Mobiluncus Caulobacter Helcococcus Psychrobacillus Campylobacter Blastomonas Wohlfahrtiimonas Thermoactinomyces Herminiimonas Tsukamurella Mycobacterium Bordetella Pichia Vibrio Iodobacter Tenacibaculum Listeria Plesiomonas Haloarcula Shewanella Paecilomyces Thauera Viridibacillus Yokenella Malassezia Novosphingobium Ornithobacterium Epidermophyton Oligella Paracoccus Aureobasidium Eubacterium Dietzia Salimicrobium Klebsiella Mycoplasma Variovorax Samsonia Schizophyllum Scopulariopsis Odoribacter Anaerotruncus Abiotrophia Burkholderia Sodalis Empedobacter Sphingopyxis Lactococcus Sphingobium Microsporum Peptoniphilus Vagococcus Beauveria Morganella Pasteurella Cedecea Bifidobacterium Micrococcus Propionimicrobium Starkeya Prevotella Histophilus Sphingomonas Acetobacter Francisella Photobacterium Propionibacterium Aneurinibacillus Arthrographis Aromatoleum Pediococcus Phoma Xenorhabdus Methylobacillus Fusarium Wolinella Bacteroides Zygosaccharomyces Cellulosimicrobium Helicobacter Rhizobium Terrabacter Ralstonia Butyricimonas Microsporum Castellaniella Borrelia Microbacterium Rheinheimera Wautersiella Saccharopolyspora Rahnella Nocardioides Gluconobacter Sphingobacterium Mannheimia Cohnella Aggregatibacter Cronobacter Lecythophora Riemerella Chaetomium Atopobium Rhizopus Acidovorax Rothia Kytococcus Chryseobacterium Alishewanella Gemella Methylobacterium Haemophilus Adlercreutzia Buttiauxella

The Market-Leading Microbiology Mass Spectrometry System

Testing for microbial pathogens and spoilage organisms is a critical function of QC laboratories in food & beverage industries. Increased demands from customers for safe and wholesome food, the increasingly complex food chain with import and export of ingredients and intermediates, and ever more stringent regulatory requirements: all these factors are constantly creating new challenges for QC providers. Additional internal factors are the need for constant improvement of production methods and process economics. Therefore, QC managers need to manage the challenge to guarantee highest product safety with short testing times at reasonable costs.

The MALDI Biotyper provides specific and reliable identification of microorganisms within minutes. Implementing the system in the microbial QC workflow can directly translate to significant cost savings by accelerated testing along the entire process chain.

Identifying microorganisms by their molecular fingerprint

The MALDI Biotyper identifies microorganisms using MALDI-TOF (Matrix-Assisted Laser Desorption Ionization/Time of Flight) Mass Spectrometry to determine the unique proteomic fingerprint of an organism.The characteristic spectrum pattern of this proteomic fingerprint is used to reliably and accurately identify a particular microorganism by matching thousands of reference spectra from microorganism strains.

Proteomic Fingerprints of Microorganisms

Aspergillus fumigatus

Bacillus subtilis

Candida krusei

Campylobacter jejuni

Microorganism Identification within Minutes

Proteomic Fingerprints of Microorganisms

Integrating MALDI Biotyper into the QC workflow results in a significant consoli-dation of resources because it replaces multiple traditional and biochemical iden-tification methods.

The MALDI Biotyper workflow has been designed to be as robust and easy to perform as possible.

Only a few simple steps are required to generate high-quality, reliable micro-organism identification.

The process of mass spectra acquisition and spectra-matching is standardized, resulting in highly accurate microorgan-ism identifications in every run.Fully traceable workflows are possiblethrough the use of barcoded MALDItarget plates.

Typically, no more than an isolated colony from a culture plate or small aliquot from a broth is required. The entire method takes only a few minutes for a single sample and less than one hour for 96 samples and provides results up to 24 hours faster than traditional methods.

After acquisition of spectral data and spectra matching the system generates an intuitive report with identification results for each sample.

The MALDI Biotyper workflow is rapid and easy-to-perform. The identification of microorganisms is fast, sensitive, and specific, and results are easy to inter-pret. In addition to cost savings from the significantly reduced confirmation times, laboratories benefit from lower costs per test compared to traditional biochemical and molecular methods.

Select anisolated colony

Transfer sample and add matrix

Add to MALDI-TOF project list

Software automatically generates MALDI-TOF spectrum

Spectrum instantly matched against reference library

to give identification

Final review and validation

Salmonella, Listeria, E. coli and Campylo-bacter are the most important pathogensbut there are a host of other pathogens and spoilage organisms that food and beverage producers need to detect and characterize.

Microbiology QC Made Easy

These include Clostridia and Cronobacter in the dairy industry, spoilage organisms such as Alicyclobacillus and heat-resis-tant molds for beverage producers, and Legionella for water supply companies.

Most samples are still collected from end products or raw materials. But there is a fast-growing demand for in-process control and environmental monitoring.

The MALDI Biotyper system can beemployed in all of these differentapplication fields and it takes just a few easy steps to identify a broad range of microorganisms with the MALDI Biotyper system.

Classification and identification are based on proteomic fingerprinting using high-througput mass-spectrometry.

The MALDI Biotyper provides signifi-cantly faster times-to-results, higher accuracy, and lower costs compared to classical methods.

More Than a Comprehensive Library

Up-to-date and traceable

The integrated library of the MBT Soft-ware comprises spectra of thousands of strains including bacteria and fungi (yeast and mold). The library is continuously maintained and updated according to strict quality-controlled procedures.Spectra are determined from:

• isolates from collaborating partners• round robin strains• ISO 9001: 2008 certified strain collection isolates

Creating your own libraries

QC laboratories that need to create their own libraries can make use of software tools to easily compile customized micro-organism entries and to share and export libraries.

These might be libraries with site-specific pathogens and / or entries for important species used for production, such as probiotic cultures, acetic acid bacteria, or fermentative yeast.

For further investigations, software tools – such as dendrogram analysis – are available.

MSP Dendrogram

Simplified dendrogram illustra-ting the separation of different Listeria groups.

The MALDI Biotyper software offers multiple sophisticated bioinformatics approaches for spectra comparison and statistical data interpretation. It enables users to optimize the classification process by creating, modifying, and organizing reference spectrum data. Advanced analysis and output options enable visualization of relationships between different groups of microorganisms and test samples using Principal Component Analysis (PCA) dendro-grams and scores and loading tables, as well as MSP dendrograms and Composite Correlation Index (CCI) matrices.

The Best Mass Spectrometers for Microbial Identification

As the pioneer and leader in MALDI-TOF technology we have used our experience to design robust, compact, high-perfor-mance platforms intended for extensive and routine use in the microbiology laboratory.

MALDI Biotyper is a modular system designed to be configured according to individual laboratory needs. Bruker offers laboratories the opportunity to choose the MALDI-TOF mass spectrometer that best fits their needs:

• The MALDI Biotyper based on bench- top microflex LT/SH MALDI-TOF mass spectrometer• The MBT “smart” system, the first microbiology mass spectrometry system with smartbeam™ lifetime* laser technology for increased speed

* Lifetime means: 500 million laser shots or seven years – whichever comes first

Space-saving systems

MALDI Biotyper mass spectrometers are true benchtop solutions requiring less than 1 meter/ 40” of space and a 110 / 220 V electrical supply, which generates minimal thermal output.

Resolution meets sensitivity

Resolution and sensitivity of the spec-trometers are tailored to the needs of microbiologists. Optimal results are achieved without the need for long and bulky instrument flight-tubes.

High run-to-run reproducibility

The quick and simple Bacterial Test Standard quality check performed before each run provides the highest standard of reproducibility.

Continuous high performance

The self-cleaning MALDI Perpetual ion source permits continuous high per-formance with minimized maintenance requirements. The integrated IR-laser allows ion source cleaning within 15 minutes in a push-button process, decreasing downtime due to tedious manual cleaning procedures.

MALDI Biotyper System Overview

Microbial Identification Applications

• Gram +/- bacteria, yeasts, filamentous fungi, mycobacteria

System Components

• Instrument: • Benchtop microflex LT/SH mass spectrometer or • Benchtop microflex LT/SH “smart” mass spectrometer with smartbeam lifetime laser technology for increased speed of analysis• Computer: MALDI Biotyper data- system running under Windows® 7• Software: MALDI Biotyper 4.0 System Software

Accessories

• MALDI Biotyper Pilot for guided sample transfer• MALDI Biotyper Galaxy automated MALDI target preparation system

Consumables and Ancillaries

• Matrix HCCA• Bacterial Test Standard (BTS)• Reusable polished stainless steel MALDI Targets 48- & 96-position with barcode• Disposable 48-position MALDI Biotarget with individual barcode

System Implementation and Qualification

• IQ and OQ-PV documentation• 21CFR part 11 support (optional)

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Bruker Daltonik GmbH

Bremen · GermanyPhone +49 (0)421-2205-0 Fax +49 (0)421-2205-103

Bruker Daltonics Inc.

Billerica, MA · USA Phone +1 (978) 663-3660 Fax +1 (978) 667-5993

ms.sales.bdal@bruker.com - www.bruker.com

MALDI Biotyper is a registered trademark of Bruker Corporation in the European Union and the USA.Windows is a registered trademark of Microsoft Corporation in the United States and other countries.

Not for use in diagnostic procedures.