fluorescence microscopy unit...
TRANSCRIPT
We cant believe its already the end of the year!
This year was filled with exciting new pro-
spects in our unit and as always, we would like
to share this with you before we end off this
year. In this newsletter, we will share the open-
ing of the CLEM platform that have been opti-
mized in conjunction with the CAF EM unit. As
always, we share some of the recent publica-
tions from our users from mid-to-end 2016. We
have some information on the new updates
with regards to the first international ISAC
workshop that we will be hosting in April 2017.
As we are ending off the year, we do have a
few announcements on the dates of the unit
closure for 2016 and opening in 2017. Please
feel free to contact us regarding projects in-
volving microscopy and flow cytometry for the
coming new year. We hope you enjoy the fes-
tive season!
On 11 October 2016, the CAF Fluorescence
Microscopy Unit and the CAF Electron Mi-
crobeam Unit hosted the Equipment Platform
Launch of the Merlin FE SEM, Super-
resolution microscopy and the new Correla-
tive Light and Electron Microscopy (CLEM)
platform.
This event
was met with
great enthu-
siasm. After
a welcoming
note from the
director of
CAF, Prof
Gary Ste-
vens, Mrs.
Lize Engelbrecht and Dr Angelique Laurie
showcased images and explained the tech-
niques behind their work
on super-resolution mi-
croscopy, electron mi-
croscopy and ultimately
CLEM. Lastly, and most
notably, Dr Lydia-Marie
Joubert, electron micros-
copy specialist from
Stanford University,
USA, presented on the
applications of CLEM in
her laboratory. She pro-
vided great insight into CLEM and its many
uses and drawbacks.
Overall, the talks were well-received and we
had a good response from the audience, with
excitement for the newer platform in CAF.
We would also like to thank Zeiss South Afri-
ca for their sponsorship for the evening.
WELCOME!
CLEM PLATFORM LAUNCH
IN THIS
ISSUE
WELCOME 1
CLEM PLATFORM
LAUNCH
1
CLEM
MICROSCOPY
2
RECENT ARTICLES 3
ISAC WORK-
SHOP UPDATE
4
YEAR-END
CLOSING
4
FLUORESCENCE MICROSCOPY UNIT I S S U E 1 1 N O V 2 0 1 6
Mrs. Gail Giordani (from Zeiss), Dr
Ben Loos, Mrs Lize Engelbrecht and
Dr Angelique Coetzer
Dr Lydia-Marie
Joubert, Stanford Uni-
versity
P A G E 2
CORRELATIVE LIGHT AND ELECTRON MICROSCOPY C
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I S S U E 1 1
Below are some example images of CLEM that were presented at the launch.
a
a
a
b
b
b c
c
a b c
Persimmon fruit sections with imperfections: a) Transmitted light image, overlaid with autofluorescence
and b) a SEM image of the same area. The sample was later analysed for elemental content at the marked
locations.
a) Fluorescence image of a neuronal cell stained for the protein α–tubulin and a nuclear dye, b) a SEM image of
the same area, and c) the overlay of the fluorescence and electron micrographs.
Electrospun polymer fibres with antimicrobial coating. a) The fluorescence indicates the distribution of the antimicrobi-
al agent on the fibres, b) while the electron micrographs show morphology of the fibres. The Shuttle and Find feature of
the ZEN 2012 software (Zeiss) is then used to overlay these images.
Cancer cells treated with viral particles. a) Two cells stained with a membrane dye (green), a nuclear dye (blue) and
Cy3 labelled viral particles (red). b) A STEM image of a thin section (70 nm) through the same cells and c) an overlay
of the images.
P A G E 3
RECENT ARTICLES C
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THE BRASSICACEAE SPECIES HELIOPHILA CORONOPIFOLIA PRODUCES ROOT BORDER-LIKE CELLS THAT PROTECT THE
ROOT TIP AND SECRETE DEFENSIN PEPTIDES
Root tip and detached border-like cells (BLCs)
stained with calcofluor white which stains cellu-
lose-enriched cell walls (A) and BLCs at higher
magnification (B). The appearance and formation
of border-like cells (BLCs) at the root tip of Heli-
ophila coronopifolia viewed under phase contrast
microscopy (C–E) are at 15-min intervals). The
arrows indicate attached BLCs. Scale bars: 40 um
(A),20 um (B), 200um (C-E)
Light microscopy observed cell
morphologies presented by B.
bruxellensis strains. Time 0 hours
(A) AWRI, (B) IWBT, (C) LO2E2;
Time 48 hours (D) AWRI, (E)
IWBT, (F) LO2E2; Time 72 hours
(G) AWRI, (H) IWBT, (I) LO2E2.
Florent Weiller, John P. Moore,*, Philip
Young, Azeddine Driouich and Melane´
A. Vivier. (2016)
ANNALS OF BOTANY
COMPARATIVE MORPHOLOGICAL CHARACTERISTICS OF THREE BRETTANOMYCES BRUXELLENSIS WINE STRAINS IN THE
PRESENCE/ABSENCE OF SULFUR DIOXIDE
Marli Louw, Maret du Toit, Her-
vé Alexandre, Benoit Divol
(2016)
INTERNATIONAL JOURNAL OF
FOOD MICROBIOLOGY , Volume
238, pp: 79–88
P A G E 4
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As we are ending the year,
kindly note that the unit will be
closed as of the 16th Decem-
ber 2016 and we will reopen
on the 9th January 2017.
Please ensure that all your data has been col-
lected and backed up before going on holiday.
Limited assistance will be available in Decem-
ber, so please speak to us if you would like to
work in December so you can receive the
necessary training. Fully trained users also
have to make arrangements to work during
the holiday.
We wish you a great festive season and a
happy 2017!.
UNIT YEAR-END CLOSING
ISAC-BD FLOW CYTOMETRY WORKSHOP 2017
As we mentioned in our previous newsletter,
the CAF Fluorescence Microscopy Unit will be
hosting the first international ISAC-BD Flow
Cytometry Workshop from the 11th to 13th
April 2017. Several international flow cytome-
try experts will be conducting workshops
which will range from basic flow cytometry
principles to specialized techniques for stem
cell biology, cell proliferation and cell death
assays and multi-parameter analysis.
We are pleased to announce that the early-
bird registrations are now open and students
will be able to submit abstracts for poster
presentation.
For more information on the ISAC-BD work-
shop, please visit the website:
http://www.isac-capetown-flowworkshop.com/
or contact Lize Engelbrecht for more infor-
mation.
Tygerberg Flow Cytometry Centre:
Mrs. Andrea Gutschmidt ([email protected])
Tel: 021 938 9400 / 9786
Contact us:
Stellenbosch Main campus
Mrs. Lize Engelbrecht ([email protected])
Ms. Rozanne Adams ([email protected])
Ms. Dumisile Lumkwana ([email protected]) Tel: 021 808 9327 / 4030
REGISTRATION IS NOW OPEN
Booking system: http://www.supersaas.com/schedule/CAF_Fluorescent_Microscopy_Unit
Websites: www.sun.ac.za/caf OR blogs.sun.ac.za/fmc