Fluorescence Test Plate

Software - Controlled Verification of Microplate Fluorometers

• Alignment of Plate Transport • Reproducibility • Linearity • Crosstalk • Quality & Position of Filters • Fluorophores in a solid plastic matrix: stable, even after 100 Million • MS Excel Evaluation Template, factory-calibrated Nominal Values

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13475 Blackhills RoadSan Diego, CA 92129Phone: 858-385-0556

Toll Free: 888-786-7835Fax: 858-630-3797

: www.akbiosource.compport@akbiosource.com

Wells A1-H9 & A12-H12 are measured at EX 360nm and EM 520nm.

Wells A10-H10 are measured at EX 320nm and EM 420nm.

Wells A11-H11 are measured at EX 480nm and EM 612nm.

egistered trademarks of Microsoft Corporation.e registered trademarks of manufacturer.e for informational purposes.

Fluorescence Test Plate

Cross Talk A positive well is surrounded by blank wells, and blank well is surrounded by positive wells, and in each case a ratio is taken to determine "spill over" or "cross talk" between wells. These are referred to as "best case" and "worst case" cross talk, respectively. Cross talk higher than 3 x 10-5 is considered "out of specification."

Alignment The plate has very deep and narrow wells in columns 1 and 12 to measure alignment.

The software evaluates the linearity and consistency of these wells, and flags readers outside defined limits as "out of alignment."

Linearity The same fluorophore used in the alignment holes is also used at the microplate standard depth in a standard curve. Replicates of 5 different sized holes create the curve, and each plate comes with "Nominal Values" measured on a calibrated "Gold Standard" reader. The software plots the standard curves as well as flags readers that are not close enough to the Gold Standard.

Filter Verification The plate also includes strips of Red and Blue fluorophore, which are used to compare signal to background at alternate wavelengths. If a filter on a given reader is incorrect or has gone bad, then either the signal will be depressed or the background will be elevated, and the reader will fail at those wavelengths.

Reproducibility The plate is measured 5 times, the data is copied into the MS Excel Evaluation Template. Custom scripts to automatically import the data are possible.

From these 5 Readings, If the CV of any well is outside the defined limit, the reader fails the reproducibility test.

Excel and MS Excel are registered trademarks of Microsoft Corporation.Pictures and literature are registered trademarks of manufacturer.They are only shown here for informational purposes.