final review-sandhya varma
TRANSCRIPT
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SOLID STATE FERMENTATION OFPADDY STRAW FOR PRODUCTION OF BIOETHANOL USING TRICHODERMA
REESEISANDHYA VARMA S. AND A.MURALIDHARAN
DEPARTMENT OF GENETIC ENGINEERING, SRM UNIVERSITY
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INTRODUCTION• Recently the demand of ethanol has increased considerably
because of its use as gasoline in addition to other application in industries which need production of alcohol on large scale ( Wati L et al.,2007)
• Abundant agricultural residue such as corn stover, wheat straw, rice straw, bagasse, etc. are used in cellulase production (Romero et al., 1999; Xia and Cen,1999; Kang et al., 2004; Wen et al., 2005; Sukumaran et al.,2009).
• Bioethanol is a clean, renewable, low green house gas emitting fuel (Wheals AE et al., 1999)
• Ethanol has a octane number of 99 which prevents pre ignition and thereby proves to be a better fuel than petrol (Wheals AE et al.,1999)
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OBJECTIVES
• Cellulolysis of paddy straw using Trichoderma reesei (MTCC*164)
• Extraction of crude extract• Distillation of crude to extract ethanol• Confirmation and Quantitative analysis of
ethanol using Gas Chromatography
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MATERIALS AND METHODSMaterials:• Trichoderma reesei (MTCC*164) procured from
IMTECH, Punjab, India.• Paddy straw collected from Potheri• Solid State Fermentation set up.• Gas chromatogram (Chemito GC 1000)• Simple Distillation UnitMethods:• Solid state fermentation• Simple Distillation• Gas Chromatography
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RESULTS AND DISCUSSION
Pictures showing solid state fermentation of paddy straw
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Pictures showing slants with Trichoderma reesei
T. reesei
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OBJECTIVE 1: Cellulolysis of paddy straw using Trichoderma reesei (MTCC*164)
Complete hydrolysis
Partial hydrolysis
Incomplete hydrolysis
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Growth of T.reesei in the walls of the bioreactor
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OBJECTIVE 2: Extraction of crude extract
Pictures showing the extraction of crude extract
Crude extract
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OBJECTIVE 3:Distillation to extract ethanol
Cooling system
Crude extract
Distillate
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Picture showing the distillate after distillation
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Ethanol
Hexane layer
water
Picture showing the separation of ethanol using hexane layer
Extraction of ethanol by solvent extraction method
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10% ethanol standard 20% ethanol standard Chromatogram showing peaks for ethanol standards
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30% ethanol standard 40% ethanol standard
Chromatogram showing peaks for ethanol sandards
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Chromatogram showing peak for the sample ethanol
OBJECTIVE 4: Quantification of ethanol using Gas Chromatography
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TABLE 1Sample Substrate
weight(grams)Equivalent broth(ml)
Duration in days
SSF1 (PS Tr) 40 40 63
SSF2 (PS Tr) 30 30 61
SSF3 (PS Tr) 20 20 57
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Sample yield of crude extract (ml)
Extract after distillation(ml)
yield of bioethanol from distillate(%)(v/v)
Total yield of bioethanol (%)(v/v)
SSF1 (PS Tr) 60 07 ml 16 2.8
SSF2 (PS Tr) 30 03 ml 15.6 2.7
SSF3 (PS Tr) 20 05 ml 15.5 2.6
TABLE 2
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STATUS REPORT
OBJECTIVES STATUS
Cellulolysis of paddy straw using Trichoderma reesei MTCC*164
completed
Extraction of crude extract completed
Distillation to extract ethanol. completed
Quantification of ethanol using Gas Chromatography
completed
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THANK YOU!!!