final draft module 3 word - ensuring specimen integrity ... › wp-content › uploads › 2017 ›...
TRANSCRIPT
1 DRAFT
Module3–EnsuringSpecimenIntegrityforViralLoadTesting
Learningobjectives:
Bytheendofthemodule,participantswillbeableto:
• Listthespecimensrejectioncriteriaforviralloadtesting
• Describethecorrectspecimencollectionproceduresforviralloadtesting
• Performcorrectproceduresforseparationofplasmafromwholeblood
• Properlypreparedriedbloodspots(DBS)forviralloadtesting
• Properlystoreandpackagespecimensfortransport
• Trackspecimenreferralstatustoensuretimelyreturnofalltestresults
Targetaudiences:allcadresinvolvedinpre‐testingsamplecollection,processing,storage,andpackaging(Clinicians,Phlebotomists,Counselors,andLaboratorians)
Pre‐requisites:Module1
Participanthandouts:3‐1,3‐2,3‐3,3‐4,3‐5,3‐6,3‐7,3‐8,3‐9,3‐10,3‐11,3‐12
Specialpreparationsbeforefacilitating:None
Icon Meaning
RefertoHandout
Customizetheslideforlocalcontext
2 DRAFT
Module‐at‐a‐glance
Segment Whatyoudo Time Handout
Moduleopening 0:05
Slides1‐2 Statethemoduleobjective.
1. SpecimenTypeusedforViralLoadTesting 0:15
Slides3‐7 Explaintheslidesaccordingtothecontentnotes. Slide8 Gaugeparticipants’knowledgewiththeKnowledgeCheck
question.
2. SpecimenCollectionandProcessing 0:45
Slides9‐11 Explaintheslidesaccordingtothecontentnotes. Slides12‐13 Gaugeparticipants’knowledgewiththeKnowledgeCheck
questions.
Slides14‐18 Explaintheslidesaccordingtothecontentnotes. 3‐1,3‐2,3‐3
Slides19‐26 Conductactivity3A:IsthisDBSValid accordingtocontentnotes.
Slide27‐28 Explaintheslideaccordingtothecontentnotes. 3‐4 Slides29‐33 Gaugeparticipants’knowledgewiththeKnowledgeCheck
questions.
3. SpecimenPackagingandTransport 1:20
Slides34‐39 Explaintheslidesaccordingtothecontentnotes. 3‐5,3‐6,3‐7,3‐8
Slides40 Conductactivity3B:PackagingSpecimensfor ShipmenttoReferralSitesaccordingtocontentnotes.
3‐6,3‐7
Slides41‐42 Explaintheslidesaccordingtothecontentnotes. 3‐9,3‐10 Slides43‐45 Gaugeparticipants’knowledgewiththeKnowledgeCheck
questions.
4. SpecimenTracking 0:15
Slides46‐48 Explaintheslidesaccordingtothecontentnotes. 3‐11
5. SpecimenRejectionCriteria 0:40
Slide49‐51 Conductactivity3C:TrackingReferralSpecimens accordingtocontentnotes.
3‐12
6. MakingandPackagingDBSfromEDTATubes(Apracticallaboratorysession)
2:30
Slide52 SessionA‐MakingofDBSspecimens 3‐2 SessionB(nextday)‐ DBSpackaging 3‐7
Moduleclosing 0:10
Slides53‐54 Inviteparticipantstosupplywordstocompleteeachkeymessage
TOTALMODULEDURATION: 6:00
3 DRAFT
SlideNumber
ContentNotesforPowerPointSlides
1. SpecimenTypeusedforViralLoadTesting
3 Heading‐TheQualityofSpecimensAffectsViralLoadTestResultsSpecimenintegrityisthecornerstoneofaqualityviralloadtestresult.Toprotectspecimenintegrity,theymustbeproperlycollectedinthecorrecttypeoftube,storedatthecorrecttemperature,properlyprocessedandwithinthepropertimeframe,transportedintherighttemperatureandpackaging.Coordinationbetweentheclinic,specimentransportmethodandthelabisimportanttoensuretimelypickupandproperdeliveryofspecimens.Thetestinglaboratorywillrejectanyspecimensthatarrivewithanincorrecttemperatureorimproperpackaging,orwithouttheappropriatepaperwork.Youwilllearnthespecimenrejectioncriterialaterinthismodulesoyouknowhowtopreventyourspecimensfrombeingrejected.
4 Heading‐KeepYourTurnAroundTimeShortTurnaroundtime(TAT)isalwaysachallenge.TherearemanystepsalongthewaythatcanimpactTATandeachstepshouldbetrackedtodeterminewhereandifthereisabreakdown;improvementprojectsshouldbeinitiatedtoeliminatethebreakdownstoreducetheTAT.
5 Heading‐MethodsforBloodCollectionThereareseveralmethodsofbloodcollection–venipuncture,fingerprick,andheelstick(forbabies). ThepreferredmethodforviralloadtestingisvenousbloodinEDTAtube. Fingerprickhasbeenutilizedforviralloadtesting.However,thismethodhaslimited
datasupportingitsvalidityandcurrentlyisnotrecommendedbymanufacturers.CountrieswhowanttousethisspecimencollectionmethodforviralloadtestingshouldevaluatethisformofspecimencollectiontoensureDBScreatedfromfingerprickisaseffectiveasvenipuncture.
HeelstickforcreationofDBSisnotcurrentlywidelyusedforviralloadtestingbutisthecornerstoneforEIDtesting.
6 Heading‐SpecimensUsedforViralLoad:PlasmaandDriedBloodSpots(DBS)Beforetesting,wholebloodcollectedmustbeprocessedintoeitherplasmaordriedbloodspot(DBS)specimensforviralloadtesting.Thegoldstandardspecimenforviralloadtestinghasbeenplasma.DBScreatedfromvenousbloodwasvalidatedonlyrecently.
7 Heading‐Plasmavs.DBSforViralLoadTesting:ProsandConsTradeoffsexistwitheachsampletype,andtheseshouldbebalancedagainstclinicalandoperationalconsiderations.Forexample,driedbloodspotsmayeliminatetheneedforcoldchaintransport,butthismayrestrictthetypeofviralloadplatformtobeusedandtheapplicableclinicalthresholds.Ontheotherhand,EDTA‐derivedplasmarequirescentrifugation.Smallandinexpensivebattery‐operatedcentrifugesmaybeasolution;however,wherelogisticsforadequatesampletransporttoalaboratorydonotexist,thiswillrequirededicatedstaffattheclinicleveltrainedinphlebotomy,sampleprocessing,storageandtransportaswellassafetyprecautionsassociatedwithsamplemanipulationandwastemanagement.AskparticipantswhataretheprosandconsofusingplasmaversusDBSforviraltestingintheirsetting.Listtheircontributionsontheflipchartbeforeshowingtheanswersontheslide.
4 DRAFT
SlideNumber
ContentNotesforPowerPointSlides
8 Heading‐KnowledgeCheckGaugeparticipants’knowledgewiththeKnowledgeCheckquestion.
2. SpecimenCollectionandProcessing
10 Heading‐ExampleofValidandInvalidVenousBloodSpecimensWholebloodsamplesreceivedintothelaboratoryshouldbeinspectedtoinsurethatthebloodisavalidspecimen.Invalidwholebloodspecimensincludespecimensthatarehemolyzedorclotted. Hemolysisoccursifthespecimenisshakentoovigorouslycausinglysisofthered
bloodcells.Thiscanhappenwithimpropermixingorduringtransportifthespecimensarepackagedorhandledinappropriately.
ClottingcanoccuriftheEDTAtubeisnotmixedimmediatelyorproperlybyinversionafterbloodcollection.
11 Heading‐ExampleofValidandInvalidPlasmaSamplesValidplasmasamplesareclearandhaveaslightyellowtintwithdefinedbuffycoatandredbloodcelllayers.Hemolyzedplasmaspecimensappearbrightpinktoredincoloraftercentrifugation.Lipemicplasmaoccurswhenthereisanelevatedamountoffatinthebloodandtheplasmaiswhiteandthick.Otherspecimensthatarecontaminatedcanhaveextralayersofunidentifiablecellsordebris.ContaminatedspecimenscouldbecausedbyissueswiththeEDTAcollectiontubeorbutterflyortubingassociatedwithbloodcollection.Besuretoinspectalloftheseitemspriortouse,butdonotopenEDTAtubes.Contaminationcanbeseenwithoutopening.
12‐13 Heading‐KnowledgeCheckGaugeparticipants’knowledgewiththeKnowledgeCheckquestions.
5 DRAFT
SlideNumber
ContentNotesforPowerPointSlides
14
Handout3‐1
Heading–PlasmausingVenousBloodAlthoughitisoutsidethescopeofthismodule,managementmustmakesurephlebotomistsarecompetentandfollowtheproperprocedureswhencollectingvenousblood.ReferthefollowingthreetoolsfromtheSLMTAcurriculum(https://www.slmta.org/tool‐kit/english)toensurephlebotomistcompetency: 701‐PhlebotomyChecklist 702‐PhlebotomyKeyCompetencies 703‐PhlebotomyPatientSurvey
PlasmaPreparation(http://www.thermofisher.com/us/en/home/references/protocols/cell‐and‐tissue‐analysis/elisa‐protocol/elisa‐sample‐preparation‐protocols/plasma‐and‐serum‐preparation.html) Collectwholebloodintocommerciallyavailableanticoagulant‐treatedtubese.g.,
EDTA‐treated(lavendertops)orcitrate‐treated(lightbluetops).HeparinizedtubescannotbeusedasitinhibitsPCRreactions.Cellsareremovedfromplasmabycentrifugationfor10minutesat1,000‐2,000xg(ideally1500)usingarefrigeratedcentrifuge.
Theresultingsupernatantisdesignatedplasma.Followingcentrifugation,itisimportanttoimmediatelytransfertheliquidcomponent(plasma)intoacleanpolypropylenetubeusingaPasteurpipette.Thesamplesshouldbemaintainedat2‐8°Cwhilehandling.Iftheplasmaisnotanalyzedimmediately,theplasmashouldbeapportionedinto0.5mlaliquots,stored,andtransportedat–20°Cforshorttermstorageof‐70Cforlonger(amonthormore)storage..Itisimportanttoavoidfreeze‐thawcyclesanddonotfreezethawmorethan3times.Sampleswhicharehemolyzed,icteric,orlipemiccaninvalidatecertaintests.
15 Heading‐CentrifugationofWholeBloodintoPlasmaCentrifugationofwholebloodatthecorrectspeedandtimeiscriticaltoproperplasmaseparation.CentrifugationcanbemeasuredusingRPMorxg(RCF).RMP(rotationsperminute)varybasedonrotorinuseandthereforisdifferentforeachtypeofrotorevenwhenutilizingthesamecentrifuge.XgorRCF(relativecentrifugalforce)isaconstantmeasurementanddoesnotvarybymanufacturerorrotor.ThereforRCFshouldbeutilizedtostandardizethespeedatwhichthetubesarespun.Astandardspeedandtimeforseparationofplasmafrombloodis1500xgfor10minutes.Thecentrifugeshouldbecleanedonaregularbasisandmustbecalibratedyearly.Inthelab,utilizetheavailableequipmenttoshowhowtotogglebetweenRPMandRCFandhowthenumbervaries.IfpossibleshowanddiscusstheuseofanomagraphtodetermineRCFwhenallyouhaveisinformationonRPM.
6 DRAFT
SlideNumber
ContentNotesforPowerPointSlides
16
Handout
3‐2
Heading–SpottingandDrying theDBSCardfromVenipunctureEDTATubesReferparticipantstothejobaide(3‐2)andwalkthemthroughthesteps.Therewillbealaboratorysessionforhands‐onpracticeofmakingtheDBSspecimens.Provideadditionaltips: TheareawhereDBSarepreparedneedstobeinside,awayfromwindanddust,not
exposedtodirectsunlightandmustbeinacleanandnon‐clutteredspace. AlwaysuseUniversalSafetyPrecautions.Thisincludes:
o Treatallbloodsamplesasthoughtheyareinfectious.o Washhands.o Weargloves,apron/labcoatandifnohoodisavailableuseafaceshieldo Disposeofwasteappropriately.
Iftherearenopipettesavailableatransferpipetteoracapillarycanbeused.Justensurethatthespotiscompletelyfilled.
Youmustchangetipsbetweeneachpatientandonlyonetubeshouldbeopenedatanygiventime.
17
Handout3‐3
Heading–SpottingandDryingtheDBSCardfromFingerPrickUsingaCapillaryTubeReferparticipantstothejobaide(3‐3)andwalkthemthroughthesteps
18 Heading‐AValidDBSSpecimenAvalidDBSspecimenshouldbelabeledwithapatientIDoruniqueidentifier,adateandthespotsshouldbecompletelyfilled.Ifthereisnotenoughbloodfor5completespots,aminimumof3completelyfilledspotsshouldbecreated.Itisokayifthebloodgoesoutsideofthecircle.
19‐26 Heading‐Activity3A:IsthisDBSValid?Readtheactivityslide(#19)beforeshowingslide#20.Allowparticipants3minutestowritedowntheirresponsesbeforeunveilingtherightanswersusingSlides#21‐#26.
27
Handout
3‐4
Heading‐HowtoPreventUnacceptableDBSSamples?Referparticipantstothejobaide(3‐4).HighlightthecriteriaforacceptableDBSandunacceptableDBSsamples.EmphasizethatunacceptableDBSsampleswillberejectedbythetestinglaboratory.DiscussthingstheycandotopreventunacceptableDBSsamples.Stressthepointsbelow:
Ensurethatallpatientinformation(nameand/oruniqueidentifier)ispresentandlegible
Ensurethedateisincluded Ensurethecirclesarecompletelyfilledin Ensurethereareatleast3godspotspercard Ensurethespotsarenotlayeredorclottedorcontaminatedwithwateroralcohol Ensurethespotsarecompletelydrypriortopackaging
7 DRAFT
SlideNumber
ContentNotesforPowerPointSlides
28 Heading‐Recommendedvs.MinimumSpecimenVolumeforViralLoadTestingTheminimumspecimenvolumeissetbecausebelowthatleveltestingcannotbedone.Whenspecimencollecteddoesnotmeetthatminimumrequirement,itwillberejected.Ontheotherhand,wewouldliketocollectmorethanjustminimumspecimenvolumeincaseretestingisrequiredduetoinstrumentfailureoroperatorerrors.
29‐33 Heading‐KnowledgeCheckGaugeparticipants’knowledgewiththeKnowledgeCheckquestions.
3. SpecimenPackagingandTransport
35
Handout
3‐5
Heading–GuidelinesforSpecimenHandlingConditionsAftercorrectlabelingandpackaging,specimensshouldbeplacedatthecorrectstoragetemperature.Specimensshouldbestoredindedicatedrefrigeratorsandfreezersandshouldnotbestoredwithreagents.Eachspecimentypehasveryspecificrequirementstoensuretheirintegrityfortestinganddifferentmanufacturesoftenhavedifferentsuggestedstorageconditions.Alwaysrefertothemanufacturerforidealspecimenstorage.Ingeneral, Wholebloodshouldbeseparatedintoplasmawithin6hoursofcollection.Ifthisis
notpossible,wholebloodcanbestoredupto24hrsat2‐8Candshouldbetransportedatthissametempwithfrozenicepacks.Wholebloodcanneverbefrozenforlateruseinviralloadtesting.
Plasmacanbestoredat2‐8Cforupto5days.Andpriortofreezing,plasmacanbetransportedatthistemperaturewithfrozenicepacks.Forstoragefrom5‐60daysplasmacanbeheldat‐20Cbutideallyplasmashouldbeplacedat‐70Cforanylongtermstorage.Onceplasmaisfrozen,itmustbetransportedfrozentoavoidfreezethawcycles.
DBSifpackagedproperlycanbestoredatambienttemperatureforupto2weeksandcanbetransportedatthissametemperature.Forstorageafter2weeks,DBSshouldbekeptat‐70C.
36 Heading–PackagingViralLoadSamplesforTransportationAllspecimentypesshouldbetransportedasdescribedutilizingthetriplepackagingsystem.Localandinternationallawsshouldbeutilizedtoavoidanytransportissues.
37
Handout
3‐6
Heading–ExampleofTriplePackagingSystemAlltriplepackagingincludes3layers. Thefirstlaterconsistsoftheprimaryspecimencontainer.Forviralloadthisisatube
orfilterpaper. Thesecondpackagingcontainercontainsabsorbentmaterialandisleakproofto
preventspillage.Thiscanbeaspeciallydesignedscrewtopcontainerorsomethinglikeaziptopbag.Oftenthesecondlayerofpackaginghasarackorsimilaritemtokeepspecimensfrommovingaroundtoomuch.
Theoutercontainershouldhavebiohazardouslabelingwhennecessary(DBSdoesnotrequirethis)andshouldcontainallrelevantformsandpaperworktoaccompanythespecimens.Theoutercontainercanconsistofaboxorcooler.Theoutsideofthe3rdcontainershouldremaincleansoastoeasilybehandledwithoutPPE.
Referparticipantstoandwalkthemthroughjobaide3‐6.
8 DRAFT
SlideNumber
ContentNotesforPowerPointSlides
38
Handout
3‐7
Heading‐PackagingWholeBloodandPlasmaforTransportReferparticipantstoandwalkthemthroughjobaide3‐7.
39
Handout
3‐8
Heading‐PackagingDBSforTransportReferparticipantstoandwalkthemthroughjobaide3‐8.TherewillbeapracticalsessionlaterwithspottingtheDBScards.
9 DRAFT
SlideNumber
ContentNotesforPowerPointSlides
40
Handout3‐6,3‐7
Heading‐Activity3B:PackagingSpecimensforShipmenttoReferralSitesReferraltestingrequiresproperpackagingandshippingofpatientspecimenstopreservetheirintegrityandsuitabilityandtoprotectallpersonsinvolvedintheirtransportation.Inthisactivity,participantslearntheimportanceofsafeandeffectivespecimenpackingandpracticeappropriatelypackingsampleswithavailablematerialsofvaryinglevelsofsophistication.
Preparationsbeforetheactivity: Setupthreeworkstations(tables),eachequippedwithonesetofmaterialsbelow
andnecessaryPPE Donotuseactualspecimens,butobtainthespecimencollectioncontainersor
materials(i.e.,glassslide)tosimulatethespecimenstobepackaged. LabelallspecimensandtheiraccompanyingtestrequisitionswithpatientID
information,date/timeofcollection,collector’sinitials,andIDnumber.Tosimplifythepatientnameselectionyoumayuse“PatientA,PatientB,etc.”
Set1(Standard–commerciallyavailable)
Testrequisitionforms
6Specimens
3bloodspecimens
3DBScards
Specimentrackingform(s)
Absorbentsheathsand/orsheeting
Clearplasticbags
PathoshieldCarton
Cellotape/ShippingTape
Scissors
Biohazardshieldemblems
Coolerboxw/icepacks
Set2(RudimentaryPackaging)
Testrequisitionforms
6Specimens
3bloodspecimens
3DBScards
Specimentrackingform(s)
Severalpapertowels
Secondarycontainer(s)
1ziptopbaglarge
1specimenrack
Severalsheetsofnewspaper
Labtapeorwhateverisavailable
Scissors
Coolerboxw/icepacks
Set3(adhocpackaging)
Testrequisitionforms
6Specimens
• 3bloodspecimens
• 3DBScards
Specimentrackingform(s)
Severalnapkinsorteatowels
Secondarycontainer(s)
Clearplasticwrap
1teaboxorsmallboxsuppliescamein(likebloodcollectionsuppliesbox)
Rubberbands,tapeorrope/stringforclosure
SomesortofIcepacks
Conducttheactivity: Divideparticipantsevenlyinto3groupsandassigneachgrouptooneworkstation UseSlide#40toprovideinstructionsfortheactivity After15minutes,debriefbyunpackingeachboxandprovideyourfeedbackmaking
sureallparticipantscanseewhatyouaredoing. Stressthat,regardlessofthesophisticationofthepackagingmaterials,itshouldstill
bepossibletoproperlypackageandshipspecimensforreferral.Fancymaterialsarenotnecessary
Totalactivitytakesabout30minutes(introducing,conducting,anddebriefing)
Formoredetail,refertoModule7,PackagingSpecimensforShipmentactivityintheSLMTAcurriculum(https://www.slmta.org/tool‐kit/english).
10 DRAFT
SlideNumber
ContentNotesforPowerPointSlides
41
Handout3‐9
Heading–GuidelinesforSampleTransportSampletransportbeginsattheclinicwherepackagingtakesplaceandendsatthetestinglaboratorywherespecimensarereceived.Thespecimensmustbeproperlypackagedaccordingtoallsafetyguidelinesandicepacksusedmustbefrozen.Transportdocumentsmustbefilledout,checkedandsignedpriortothehandofftothetransportdriver.Thetransportdrivermustthenacknowledgereceiptofthespecimens.Thedriversmustbetrainedinsafepracticesforbloodbornepathogensandhavecontactinformationifaspilloccursorbetrainedandhavethenecessarymartialstodealwithaspill.Uponarrivalatthetestingfacility,specimensshouldbecheckedforanyissuespriortothedriverleavingthefacility.Referparticipantstojobaide3‐9.Walkthemthroughthehandoutandansweranyquestionstheymayhave.
42
Handout3‐10
Heading‐ChainofCustodyforSampleTransportTransportdocumentsmustbefilledtomaintainandprovechainofcustody.Eachstepintheprocessmustprovideasignaturetoacknowledgeandacceptresponsibilityforthischainofcustody.Dateandtimeshouldberecodedateveryhandoff.Thepackageshouldinspectedateveryexchangetoensuretherearenoissueswithleakageorspills.Referparticipantstojobaide3‐9.Walkthemthroughthehandoutandansweranyquestionstheymayhave.
43‐45 Heading‐KnowledgeCheckGaugeparticipants’knowledgewiththeKnowledgeCheckquestions.
4. SpecimenTracking
47
Handout3‐11
Heading‐KeepingTrackofAllReferralSamplesatClinicsThereshouldbeadesignatedindividualateachclinicresponsibleforcheckingtodetermineifsampleswerereceivedatthelaboratoryinatimelyfashionandingoodcondition.Thisindividualoranothershouldalsobethepointofcontactforthelabforissueswithspecimensandreturnofresults.Asinglepointofcontactmakesitmucheasierforlabstoknowwhotocommunicatewithwhenneeded.Forthesamereason,asinglepointofcontactatthelaboratoriesfortheclinicshouldalsobeestablished.Referparticipantstojobaide3‐11.Walkthemthroughthehandoutandansweranyquestionstheymayhave.
48 Heading‐ClinicsShouldRoutinelyTrackTheseIndicatorsTrackingtheseindicatorswillhelpensurethatallspecimenscollectedareaccountedforandresultswillbereturnedforpatientmanagement.
5. SpecimenRejectionCriteria
11 DRAFT
SlideNumber
ContentNotesforPowerPointSlides
50 Heading‐Activity3C:SampleRejectionCriteriaAssuringthatspecimensareacceptableiscriticaltothequalityofviralloadtesting.Inthisactivityparticipantswillbedividedinto3groups,eachassignedtodevelopalistofsamplerejectioncriteriaforwholeblood,plasma,orDBS.After20minutes,debriefbyconductingalargegroupdiscussionofeachgroup’slist.Provideyourfeedbackandpulloutallitemspertainingtoallthreespecimentypesandwritethemonaseparateflipchartpaperunder“General.”
51
Handout3‐12
Heading–SampleRejectionCriteriaReviewhandout3‐12toconcludeactivity3C.
LaboratoryPracticalSession
52
Handout3‐2,3‐8
Heading‐MakingandPackagingDBS fromEDTATubes‐ APracticalSession
SuppliesneededformakingDBS Suppliesneededforpackaging
Remnantwholebloodsamples 200uLpipettesandtips,ortransfer
pipettes* DBScards(twoforeachperson) Pensforlabeling Serologicalpipettesorsomethingto
utilizetokeepDBSoffthebenchtopwhenspotting
Dryingracks AllassociatedPPE Preferablyhoodtoworkin*Iftherearenopipettesavailable,atransferpipetteoracapillarycanbeused.Justensurethatthespotiscompletelyfilled.
Ziptopairtightbags Siliciapackets‐1perdbs Humiditycards Glassinepaper PPE
Emphasizethattipsmustbechangedbetweeneachpatientandonlyonetubeshouldbeopenedatanygiventime.
1. WalktheparticipantsthroughJobAide3‐2andthenhaveeachparticipantcreatetheirownbloodspotwithproperPPEandtechnique.Splitintopairsorgroupsof3andhavethemcritiqueeachother.
2. Comebacktogetherasagroupanddiscusstheissues/difficultiesifany.Thispracticalsessionshouldtakeapproximately30minutestocomplete.
3. AfterallowingDBStodryovernight,breakupintothesamegroupsfromthepreviousdayandfollowingJobAide3‐8topackagetheDBScards.Allowtimeforparticipantstocritiqueeachother.Comebacktogetherasagroupanddiscusstheissues/difficultiesifany.Thispracticalsessionshouldtakeapproximately30minutestocomplete
53‐54 Heading–Module6:KeymessagesInviteparticipantstosupplywordstocompleteeachkeymessage.
12 DRAFT
Handout3‐1
13 DRAFT
Handout3‐2
14 DRAFT
Handout3‐3
15 DRAFT
Handout3‐4
16 DRAFT
Handout3‐5
17 DRAFT
Handout3‐6
Triple Packaging System
PrimaryReceptacles
Containsthespecimen
Mustbewatertightandleakproof
Mustbeappropriatelylabeledastocontent.
Wrappedinenoughabsorbentmaterialtoabsorballfluidincaseofbreakageorleakage.SecondaryPackaging
Enclosesandprotectstheprimaryreceptacle
Mustbewatertightandleakproof
Severalwrappedprimaryreceptaclesmaybeplacedinasinglesecondarypackaging.
ContainsicepacksifneededOuterPackaging
Protectssecondarypackagingfromphysicaldamagewhileintransit Containsspecimendataforms,letters,andothertypesofinformationthatidentifyordescribethe
specimenandidentifytheshipperandreceiver,andanyotherdocumentationrequired.
Mustbeasturdycontainerwithalatchorabletobetapedshut
18 DRAFT
Handout3‐6
Specimen Packaging and Transport
Utilize PPE when packaging specimens. Ensure specimens are in the appropriate transport media (primary containers) for the specimen
collected and the test requested (primary containers). Ensure that primary containers will not leak
Determine the requirements temperature (ambient temperature vs refrigerated) and the
referral timeframe (i.e., 6 hours) for the specimen collect and the test requested. Consult the driver/courier schedule to ensure that the sample will reach the testing center
within the necessary referral timeframe. Place cold packs on the bottom (ideally) or sides of a secure leak-proof secondary container to
properly preserve the specimens during shipping (specimens shipped at ambient temperature may not require cool packs, although it is often still advisable in warm climates.)
Place the primary container(s) in the secondary container with sufficient absorbent material—
paper towels, cotton balls, commercial products—to absorb the entire contents of the primary containers.
– Ambient temperature specimens can be transported in the same secondary packaging as refrigerated specimens, but should be packed as far away from the cool pack as possible and be insulated by at least one layer of absorbent material.
Ensure secondary container(s) is labeled properly with a biohazard sticker or stamp. Place secondary container(s) in an outer shipping container that can be secured with a screw
top, latch mechanism or sealed with tape. Place test requisition forms in a plastic sheath (if possible) or zip top bag inside the outer
shipping container with specimen tracking form. Confirm that the contact information for the laboratory is clearly marked on the outer shipping
packaging and/or in paperwork inside the outer packaging Note the date and time of pick-up on the specimen tracking form/transport log and/or the
driver/courier logbook. Ensure that the drivers/couriers have received safety training in the transportation of
specimens. Disinfect the bench where the specimens were packaged.
19 DRAFT
Handout3‐6
Cross Section of Refrigerated Specimen Packaging
Outer Container
Secondary Container
Cool Pack
Ambient or room temperature specimens
Refrigerated specimens
Paper towels /
absorbent material
20 DRAFT
Handout3‐7
21 DRAFT
Handout3‐8
22 DRAFT
Handout3‐9
23 DRAFT
Handout3‐10
24 DRAFT
Handout3‐11
25 DRAFT
Handout3‐12