figure s1 approximate positions of primers listed in supplementary table s1 for f3h (a), tdic101 (b)...
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Figure S1 Approximate positions of primers listed in Supplementary Table S1 for F3‘H (a), Tdic101 (b) and dTdic102 (c). Black arrow heads show primers and their orientation. Coding regions (shaded boxes) in F3'H, the transposase (black bar) in Tdic101 and the TIRs (terminal inverted repeats, open triangles) are indicated.
a
b c
9
15 1916 18
137
4
1
2
(G)HindIII HindIII
3 56
8
10
14
Tdic101 insertion site
1720
21
23
HindIII
11
122224
2526
27 28
29
30
3132
33
F3‘H
Tdic101 dTdic102
0.81.1
23.1
6.6
2.3
9.4
2.0
(kbp)
MPu MPi 1 2 3 4 5 6 7 8
Fig. S2 Analysis of the vacated site of the 4.3 kb (Tdic101) insertion in the F3‘H gene. PCR products are shown that are obtained with gene-specific primers on DNAs isolated from six different reverted, purple regions emerging in MPi flowers (1-6) or from pink regions in partly reverted variegated MPi flowers (7-8). Analysis by PCR of DNA isolated from MPu and MPi is shown and the migration of molecular weight markers is indicated on the left.
Fig. S3 Schematic structure of the putative Tdic101 transposase (TPase). Black boxes represent the six conserved hAT-transposase motifs reported by Rubin et al. (2001). The number of amino acids (aa) separating these motifs in the Tdic101 TPase are indicated.
A; CKFNLTADVWDS D; VLDLLLWWKGQB; HVRCACHVLNLCV E; LPVMSAMAQDFLSIQVSSVASERAFSC; TRWNSMYIML F; SKRVLDEKRTSLRSDTLEMCVCYKDW
A B C D E F
88 aa 50 aa 226 aa 3 aa 1 aa320 aa 28 aa
TPase (814 aa)