Thy-Thy1 Thy-Thy 2 Thy-Thy 3 Thy-Noc Shake

A

B

Figure 10

0 2 6 10

14

20

0 2 4 6 8 10

12

15

18

22

26

32

40

0 1 3 5 7 9 11

13

15

17

19

21

23

25

27

29

31

33

35

37

39

41

43

45

0 4 8 12

16

20

24

28

32

36

0 2 6 10

14 hrs.

Thy-Thy1 Thy-Thy 2 Thy-Thy 3 Thy-Noc Shake

MITOTIC GRADE= Not Determined= Mitotic Grade 1= Mitotic Grade 2= Mitotic Grade 3

Cell CycleBreast tumorsCell lines

Lymphoma and Cell lines Cell Cycle

CDC20 CDC20 cell division cycle 20, S. cerevisiae, homolog Hs.82906 BIRC5 baculoviral IAP repeat-containing 5 survivin Hs.1578 KIAA0008 KIAA0008 gene product Hs.77695 MGC5350 hypothetical protein MGC5350 Hs.71331 E2-EPF ubiquitin carrier protein Hs.174070 ESTs, Weakly similar to CGHU7L collagen alpha 1III chain precursor [H.sapiens] Hs.19322 GGH gamma-glutamyl hydrolase conjugase, folylpolygammaglutamyl hydrolase Hs.78619 TROAP trophinin associated protein tastin Hs.171955 PGD phosphogluconate dehydrogenase Hs.75888 DJ742C19.2 phorbolin similar to apolipoprotein B mRNA editing protein Hs.226307 TARS threonyl-tRNA synthetase Hs.84131 PTTG1 pituitary tumor-transforming 1 Hs.252587 MGP matrix Gla protein Hs.279009 LMNB2 lamin B2 Hs.76084 LOC51154 60S acidic ribosomal protein PO Hs.274201 CCT5 chaperonin containing TCP1, subunit 5 epsilon Hs.1600 BYSL bystin-like Hs.106880 ESTs Hs.294083 PES1 pescadillo zebrafish homolog 1, containing BRCT domain Hs.13501 LOC51073 CGI-28 protein Hs.279652 PA2G4 proliferation-associated 2G4, 38kD Hs.5181 FLJ20485 hypothetical protein Hs.98806 NPM1 nucleophosmin nucleolar phosphoprotein B23, numatrin Hs.9614 SNRPB small nuclear ribonucleoprotein polypeptides B and B1 Hs.83753 E2F3 E2F transcription factor 3 Hs.1189 NME1 non-metastatic cells 1, protein NM23A expressed in Hs.118638 SNRPB2 small nuclear ribonucleoprotein polypeptide B’’ Hs.82575 EEF1E1 eukaryotic translation elongation factor 1 epsilon 1 Hs.298581 YARS tyrosyl-tRNA synthetase Hs.239307 PSMC4 proteasome prosome, macropain 26S subunit, ATPase, 4 Hs.211594 Homo sapiens mRNA; cDNA DKFZp434B0425 from clone DKFZp434B0425 Hs.103305 LOC55977 **hypothetical protein 24636 Hs.4187 POLR3K polymerase RNA III DNA directed polypeptide K 12.3 kDa Hs.110857 CCNE1 cyclin E1 Hs.9700 CLNS1A chloride channel, nucleotide-sensitive, 1A Hs.84974 NASP nuclear autoantigenic sperm protein histone-binding Hs.243886 UNG uracil-DNA glycosylase Hs.78853 MCM6 minichromosome maintenance deficient mis5, S. pombe 6 Hs.155462 ADE2H1 multifunctional polypeptide similar to SAICAR synthetase and AIR carboxylase Hs.117950 E2F1 E2F transcription factor 1 Hs.96055 CDC6 CDC6 cell division cycle 6, S. cerevisiae homolog Hs.69563 ORC1L origin recognition complex, subunit 1 yeast homolog-like Hs.17908 MCM5 minichromosome maintenance deficient S. cerevisiae 5 cell division cycle 46 Hs.77171 PCNA proliferating cell nuclear antigen Hs.78996 MCM3 minichromosome maintenance deficient S. cerevisiae 3 Hs.179565 PCNA proliferating cell nuclear antigen Hs.78996 FEN1 flap structure-specific endonuclease 1 Hs.4756 RFC4 replication factor C activator 1 4 37kD Hs.35120 PKMYT1 membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase Hs.77783 DDX11 DEAD/H Asp-Glu-Ala-Asp/His box polypeptide 11 S.cerevisiae CHL1-like helicase Hs.27424 POLE polymerase DNA directed, epsilon Hs.166846 DDX11 **DEAD/H Asp-Glu-Ala-Asp/His box polypeptide 11 S.cerevisiae CHL1-like helicase Hs.27424 IFIT1 **interferon-induced protein with tetratricopeptide repeats 1 Hs.20315 DHFR dihydrofolate reductase Hs.83765 TYMS thymidylate synthetase Hs.82962 DNMT1 DNA cytosine-5--methyltransferase 1 Hs.77462 ESTs, Weakly similar to S13495 pregnancy zone protein [H.sapiens] Hs.110080 DHFR dihydrofolate reductase Hs.83765 EZH2 enhancer of zeste Drosophila homolog 2 Hs.77256 MYBL2 v-myb avian myeloblastosis viral oncogene homolog-like 2 Hs.179718 PRIM1 primase, polypeptide 1 49kD Hs.82741 PLG plasminogen Hs.75576 RRM2 ribonucleotide reductase M2 polypeptide Hs.75319 FLJ10210 hypothetical protein FLJ10210 Hs.183639 RRM1 ribonucleotide reductase M1 polypeptide Hs.2934 LRP8 low density lipoprotein receptor-related protein 8, apolipoprotein e receptor Hs.54481 CDC7L1 CDC7 cell division cycle 7, S. cerevisiae, homolog-like 1 Hs.28853 TUBB tubulin, beta polypeptide Hs.179661 MTHFD2 methylene tetrahydrofolate dehydrogenase NAD+ dependent, methenyltetrahydrofolate cyclohydrolase Hs.154672 CTPS CTP synthase Hs.251871 TIMP1 tissue inhibitor of metalloproteinase 1 erythroid potentiating activity, collagenase inhibitor Hs.5831 CDC2 cell division cycle 2, G1 to S and G2 to M Hs.184572 KNSL2 kinesin-like 2 Hs.20830 KIAA0056 KIAA0056 protein Hs.13421 TRIP TRAF interacting protein Hs.21254 TOP2A topoisomerase DNA II alpha 170kD Hs.156346 TOP2A topoisomerase DNA II alpha 170kD Hs.156346 HMG2 high-mobility group nonhistone chromosomal protein 2 Hs.80684 CCNF cyclin F Hs.1973 XPOT exportin, tRNA nuclear export receptor for tRNAs Hs.85951 TMPO thymopoietin Hs.11355 CDC25C cell division cycle 25C Hs.656 KNSL5 kinesin-like 5 mitotic kinesin-like protein 1 Hs.270845 KIAA0074 KIAA0074 protein Hs.1192 PSMD12 proteasome prosome, macropain 26S subunit, non-ATPase, 12 Hs.4295 TRIP13 thyroid hormone receptor interactor 13 Hs.6566 MAD2L1 MAD2 mitotic arrest deficient, yeast, homolog-like 1 Hs.79078 CCNA2 cyclin A2 Hs.85137 KPNA2 karyopherin alpha 2 RAG cohort 1, importin alpha 1 Hs.159557 FLJ22624 hypothetical protein FLJ22624 Hs.166425 NUDT1 nudix nucleoside diphosphate linked moiety X-type motif 1 Hs.388 OIP2 Opa-interacting protein 2 Hs.274170 FOXM1 forkhead box M1 Hs.239 PMSCL1 **polymyositis/scleroderma autoantigen 1 75kD Hs.91728 KIAA0042 KIAA0042 gene product Hs.3104 CHEK1 CHK1 checkpoint, S.pombe homolog Hs.20295 STK15 serine/threonine kinase 15 Hs.48915 CENPF centromere protein F 350/400kD, mitosin Hs.77204 HMMR hyaluronan-mediated motility receptor RHAMM Hs.72550 CDC25B cell division cycle 25B Hs.153752 FLJ11252 hypothetical protein FLJ11252 Hs.23495 CENPE centromere protein E 312kD Hs.75573 CDC25B cell division cycle 25B Hs.153752 MCM4 minichromosome maintenance deficient S. cerevisiae 4 Hs.154443 CKS2 CDC28 protein kinase 2 Hs.83758 BUB1 budding uninhibited by benzimidazoles 1 yeast homolog Hs.98658 CKS2 CDC28 protein kinase 2 Hs.83758 TSN translin Hs.75066 MAPK13 mitogen-activated protein kinase 13 Hs.178695 CCNB1 cyclin B1 Hs.23960 PLK polo Drosophia-like kinase Hs.77597 KIAA0159 chromosome condensation-related SMC-associated protein 1 Hs.5719

CDC20 CDC20 cell division cycle 20, S. cerevisiae, homolog Hs.82906BIRC5 baculoviral IAP repeat-containing 5 survivin Hs.1578NEK2 NIMA never in mitosis gene a-related kinase 2 Hs.153704VBP1 von Hippel-Lindau binding protein 1 Hs.198307FLJ20727 hypothetical protein FLJ20727 Hs.300700MNAT1 menage a trois 1 CAK assembly factor Hs.82380CDKN3 cyclin-dependent kinase inhibitor 3 CDK2-associated dual specificity phosphatase Hs.84113TM4SF1 transmembrane 4 superfamily member 1 Hs.3337TCF3 transcription factor 3 E2A immunoglobulin enhancer binding factors E12/E47 Hs.101047HPRT1 hypoxanthine phosphoribosyltransferase 1 Lesch-Nyhan syndrome Hs.82314SSI-1 JAK binding protein Hs.50640AKAP10 A kinase PRKA anchor protein 10 Hs.75456NT5B 5’-nucleotidase purine, cytosolic type B Hs.138593SCAMP1 secretory carrier membrane protein 1 Hs.31218CDKN1C cyclin-dependent kinase inhibitor 1C p57, Kip2 Hs.106070CDKN2A cyclin-dependent kinase inhibitor 2A melanoma, p16, inhibits CDK4 Hs.1174UBL1 ubiquitin-like 1 sentrin Hs.81424HDGF hepatoma-derived growth factor high-mobility group protein 1-like Hs.89525UBL1 ubiquitin-like 1 sentrin Hs.81424PA2G4 proliferation-associated 2G4, 38kD Hs.5181GRSF1 G-rich RNA sequence binding factor 1 Hs.79295CLPP ClpP caseinolytic protease, ATP-dependent, proteolytic subunit, E. coli homolog Hs.74362NDUFB7 NADH dehydrogenase ubiquinone 1 beta subcomplex, 7 18kD, B18 Hs.661ADSL adenylosuccinate lyase Hs.75527PCDHGC3 protocadherin gamma subfamily C, 3 Hs.173310NME1 non-metastatic cells 1, protein NM23A expressed in Hs.118638NME2 non-metastatic cells 2, protein NM23B expressed in Hs.275163LDHA lactate dehydrogenase A Hs.2795TCEB2 transcription elongation factor B SIII, polypeptide 2 18kD, elongin B Hs.172772PPP2R1A protein phosphatase 2 formerly 2A, regulatory subunit A PR 65, alpha isoform Hs.173902ATP5J ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F6 Hs.73851GART phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase Hs.82285DJ37E16.5 hypothetical protein dJ37E16.5 Hs.5790GMPS guanine monphosphate synthetase Hs.5398PGAM1 phosphoglycerate mutase 1 brain Hs.181013CHAF1A chromatin assembly factor 1, subunit A p150 Hs.79018E2F1 E2F transcription factor 1 Hs.96055MCM2 minichromosome maintenance deficient S. cerevisiae 2 mitotin Hs.57101CSE1L chromosome segregation 1 yeast homolog-like Hs.90073MCM4 minichromosome maintenance deficient S. cerevisiae 4 Hs.154443LOC51659 HSPC037 protein Hs.108196CDK4 cyclin-dependent kinase 4 Hs.95577RPA3 replication protein A3 14kD Hs.1608RAD51 RAD51 S. cerevisiae homolog E coli RecA homolog Hs.23044RFC4 replication factor C activator 1 4 37kD Hs.35120 ESTs Hs.111458CDK2 cyclin-dependent kinase 2 Hs.19192FANCG Fanconi anemia, complementation group G Hs.8047PKMYT1 membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase Hs.77783CASP7 caspase 7, apoptosis-related cysteine protease Hs.9216RAD54L RAD54 S.cerevisiae-like Hs.66718MTHFD1 methylenetetrahydrofolate dehydrogenase NADP+ dependent, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase Hs.172665DHFR dihydrofolate reductase Hs.83765DNMT1 DNA cytosine-5--methyltransferase 1 Hs.77462PRKDC protein kinase, DNA-activated, catalytic polypeptide Hs.155637DUT dUTP pyrophosphatase Hs.82113STK18 serine/threonine kinase 18 Hs.172052RRM1 ribonucleotide reductase M1 polypeptide Hs.2934MMP12 matrix metalloproteinase 12 macrophage elastase Hs.1695TK1 thymidine kinase 1, soluble Hs.105097TUBG1 tubulin, gamma 1 Hs.21635POLQ polymerase DNA directed, theta Hs.241517CDC2 cell division cycle 2, G1 to S and G2 to M Hs.184572DTYMK deoxythymidylate kinase thymidylate kinase Hs.79006LMNB1 lamin B1 Hs.89497TOP2A topoisomerase DNA II alpha 170kD Hs.156346KIAA0175 KIAA0175 gene product Hs.184339STK12 serine/threonine kinase 12 Hs.180655CKS1 CDC28 protein kinase 1 Hs.77550CCNF cyclin F Hs.1973KNSL5 kinesin-like 5 mitotic kinesin-like protein 1 Hs.270845MAD2L1 MAD2 mitotic arrest deficient, yeast, homolog-like 1 Hs.79078CCNA2 cyclin A2 Hs.85137MKI67 antigen identified by monoclonal antibody Ki-67 Hs.80976CENPA centromere protein A 17kD Hs.1594SLA Src-like-adapter Hs.75367MKI67 antigen identified by monoclonal antibody Ki-67 Hs.80976TACC3 transforming, acidic coiled-coil containing protein 3 Hs.104019STK12 serine/threonine kinase 12 Hs.180655FOXM1 forkhead box M1 Hs.239AOX1 aldehyde oxidase 1 Hs.174151CKS1 CDC28 protein kinase 1 Hs.77550TOPK PDZ-binding kinase; T-cell originated protein kinase Hs.104741CHEK1 CHK1 checkpoint, S.pombe homolog Hs.20295CENPF centromere protein F 350/400kD, mitosin Hs.77204HMMR hyaluronan-mediated motility receptor RHAMM Hs.72550 Homo sapiens dystrobrevin, beta DTNB, mRNA Hs.326682CKS2 CDC28 protein kinase 2 Hs.83758BUB1 budding uninhibited by benzimidazoles 1 yeast homolog Hs.98658CKS2 CDC28 protein kinase 2 Hs.83758CCNB1 cyclin B1 Hs.23960Hs.77597 DUT dUTP pyrophosphatase Hs.82113INPPL1 inositol polyphosphate phosphatase-like 1 Hs.75339NEK2 NIMA never in mitosis gene a-related kinase 2 Hs.153704RAN RAN, member RAS oncogene family Hs.10842