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☺OURNAL OF MAHANAKORN VETERINARY MEDICINE

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»·è 4 ©ºÑº·è 1 Á¡ÃÒ¤Á - ÁÔ¶Ø¹ÒÂ¹ 2552ISSN : 1905 - 7571

http://www.vet.mut.ac.th

☺ournal of Mahanakorn Veterinar Medicine

Vol. 4 No.1 ☺anuar - ☺une 2009

2


ÇÒÃÊÒÃ·Ò§ÇÔªÒกÒÃ¢Í§¤³ÐÊÑµÇá¾·ÂÈÒÊµÃì ÁËÒÇÔ·ÂÒÅÑÂà·¤â¹âÅÂÕÁËÒ¹¤Ã Ë¹Í§¨ÍกกÃØ§à·¾ÁËÒ¹¤Ã ¨Ñ´·Ó¢Öé¹´éÇÂÇÑµ¶Ø»ÃÐÊ§¤ì à¾×èÍà¼Âá¾Ãè¼Å§Ò¹¤é¹¤ÇéÒÇÔ¨ÑÂ·Ò§ÇÔªÒกÒÃ ÃÇÁ¶Ö§กÔ¨กÃÃÁµèÒ§æ ·ÕèàกÕèÂÇ¢éÍ§กÑºÊÒ¢ÒÊÑµÇá¾·ÂÈÒÊµÃì ÊÑµÇºÒÅ áÅÐÊÒ¢Ò·ÕèàกÕèÂÇ¢éÍ§ Êè§àÊÃÔÁ¤ÇÒÁÊÑÁ¾Ñ¹¸ìÍÑ¹´ÕÃÐËÇèÒ§¹ÑกÇÔªÒกÒÃ·ÕèàกÕèÂÇ¢éÍ§กÑºÇÔªÒªÕ¾ÊÑµÇá¾·Âì ÊÑµÇºÒÅ áÅÐà¾×èÍà¼Âá¾ÃèàกÕÂÃµÔ¤Ø³¢Í§¤³ÐÊÑµÇá¾·ÂÈÒÊµÃì ÁËÒÇÔ·ÂÒÅÑÂà·¤â¹âÅÂÕÁËÒ¹¤Ã

ÊÓ¹Ñก§Ò¹ : ¤³ÐÊÑµÇá¾·ÂÈÒÊµÃì ÁËÒÇÔ·ÂÒÅÑÂà·¤â¹âÅÂÕÁËÒ¹¤Ã 140 ¶. àª×èÍÁÊÑÁ¾Ñ¹ ì Ë¹Í§¨Íก กÃØ§à·¾ÁËÒ¹¤Ã 10530

กÓË¹´ÍÍกà¼Âá¾Ãè : »ÕÅÐ 2 ©ºÑº

¤èÒÊÁÒªÔก : 1 »Õ 140 ºÒ·2 »Õ 280 ºÒ·3 »Õ 420 ºÒ·µÅÍ´ªÕ¾ 1,000 ºÒ·ÃÒ¤Ò©ºÑºÅÐ 70 ºÒ·

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¤³Ð·Õè»ÃÖกÉÒÍ ÔกÒÃº ÕÁËÒÇÔ·ÂÒÅÑÂà·¤â¹âÅÂÕÁËÒ¹¤Ã¤³º Õ¤³ÐÊÑµÇá¾·ÂÈÒÊµÃì

ºÃÃ³Ò ÔกÒÃÍ.¹.Ê¾.´Ã. ¨ÓÅÍ§ ÁÔµÃªÒÇä·Â Ê¾.º., M.Sc., Ph.D.

¼ÙéªèÇÂºÃÃ³Ò ÔกÒÃÍ. ÃÑªกÄª àÅÔÈÀÑ·ÃâกÁÅ Ç·.º., Ç·.Á.Í.¹.Ê¾. ÊÁªÒÂ Ñ¹·ÃìàกÉ Ê¾.º.

กÍ§ºÃÃ³Ò ÔกÒÃÍ.¹.Ê¾.´Ã. ·¹§ÈÑก Ôì ÁÐÁÁ Ê¾.º., Ph.D.

Í.¹.Ê¾.´Ã. ÊÓÃÒ ºÃÃ³ ÔÃกØÅ Ê¾.º., Dr.med.vet.

Í.Ê¾..´Ã. ÍÒÃÂÒ Ê×º¢Óà¾ªÃ Ê¾.º., Ph.D.

Í.¹.Ê¾.´Ã. à¨É®Ò ÃØé§ÀÙè»ÃÐ ÔÉ°ì Ê¾.º., Dr.med.vet.

Í.Ê¾.. ¾Ô¹Ô ´Ò ªÐÍØèÁ¼Å Ê¾.º., Ç·.Á.Í.Ê¾.. ÊØกÑÒ ¼ÅÔµกØÅ Ê¾.º., Ç·.Á.Í. ØÌÒÀÒ ÊÍ¹กÅÔè¹ Ç·.º., Ç·.Á.Í.Ê¾.. Ñª®Ò¾Ã äªÂ¤Ø³ Ê¾.º., Ç·.Á.Í.Ê¾.. ÔµÃÒ Ê¹ÔÊØÃÔÇ§Éì Ê¾.º.Í.Ê¾.. ÇÃÒ Õ ¾Ø·¸âกÊÑÂ Ê¾.º.Í.¹.Ê¾. ªÑÂ³Ã§¤ì ÀÙÁÔÃÑµ¹»ÃÐ¾Ô³ Ê¾.º., Ç·.Á.Í. ·ÑÈ¹ÕÂì µÃÑÂÃÑµ¹ìÍÀÔÇÑ¹ Ç·.º., Ç·.Á.Í.Ê¾.. ÇÃÔÊÊÒ áÊ§áกéÇ Ê¾.º.Í.Ê¾.. °ÔµÒÃÕÂì àÅÒËÃÑªµ¸Ò¹Ô¹·Ãì Ê¾.º.Í.Ê¾.. ÊØÀÃÑµ¹ì ÇÃÃ³ÈÔÅ»ì Ê¾.º.



4

Journal of Mahanakorn Veterinary Medicine

Editorial Advisory Board

President of Mahanakorn University of Technology

Dean of Faculty of Veterinary Medicine

Editor

Jamlong Mitchaothai D.V.M., M.Sc., Ph.D.

Assistant Editor

Rachakris Lertpatarakomol B.Sc., M.Sc.

Somchai Chanket D.V.M.

Editorial Board

Thanongsak Mamom D.V.M., Ph.D.

Sumrarn Bunnajirakul D.V.M., Dr.med.vet.

Araya Suepkhampet D.V.M., Ph.D.

Jetsada Rungpupradit D.V.M., Dr.med.vet.

Phinidda Cha-umphol D.V.M., M.Sc.

Sukanya Phalitakul D.V.M., M.Sc.

Chulabha Sonklein B.Sc., M.Sc.

Thuchadaporn Chaikhun D.V.M., M.Sc.

Jitra Sanisuriwong D.V.M.

Waradee Buddhakosai D.V.M.

Chainarong Phumratanaprapin D.V.M., M.Sc.

Tassanee Triratapiwan B.Sc., M.Sc.

Warissa Sangkaew D.V.M.

Titaree Laoharatchatathanin D.V.M.

Supparat Wannasilp D.V.M.

5


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5. ÃÈ.¹.Ê¾.´Ã. ³Ã§¤ì กÔ ¾Ò³ÔªÂì âÃ§¾ÂÒºÒÅÊÑµÇìÊÇ¹ËÅÇ§ 34/2 Á.5 ¶.ÊØ¢ØÁÇÔ· 103 á¢Ç§Ë¹Í§ºÍ¹ à¢µ»ÃÐàÇÈ กÃØ§à·¾ÁËÒ¹¤Ã

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7. ÃÈ.¹.Ê¾.´Ã. ·ÇÕÈÑก Ôì Êè§àÊÃÔÁ ÀÒ¤ÇÔªÒ¾ÂÒ ÔÇÔ·ÂÒ ¤³ÐÊÑµÇá¾·ÂÈÒÊµÃìÁËÒÇÔ·ÂÒÅÑÂàกÉµÃÈÒÊµÃì ÇÔ·ÂÒà¢µกÓá¾§áÊ¹ ÍÓàÀÍกÓá¾§áÊ¹ Ñ§ËÇÑ ¹¤Ã»°Á

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10. ÃÈ.Ê¾..´Ã. ´Ç§ÊÁÃ ÊØÇÑ±²¹ ÀÒ¤ÇÔªÒÊÑµÇºÒÅ ¤³ÐÊÑµÇá¾·ÂÈÒÊµÃì ØÌÒÅ§กÃ³ìÁËÒÇÔ·ÂÒÅÑÂ »·ØÁÇÑ¹ กÃØ§à·¾ÁËÒ¹¤Ã

11. Í.¹.Ê¾.´Ã. กÃก® §Ò¹Ç§Èì¾Ò³ÔªÂì ÊÒ¢ÒÇÔªÒ¾ÃÕ¤ÅÔ¹ÔกÊÑµÇá¾·Âì ¤³ÐÊÑµÇá¾·ÂÈÒÊµÃìÁËÒÇÔ·ÂÒÅÑÂàªÕÂ§ãËÁè Ñ§ËÇÑ àªÕÂ§ãËÁè



6

ÊÑµÇá¾·ÂìÁËÒ¹¤ÃÊÒÃ»Õ·Õè 4 ©ºÑº·Õè 1 ÁกÃÒ¤Á � ÁÔ¶Ø¹ÒÂ¹ 2552ISSN : 1905 � 7571 http://www.vet.mut.ac.th

ÊÒÃºÑ§Ò¹ÇÔ ÑÂ

กÒÃà¾ÒÐàÅÕéÂ§àª×éÍ¹ÕâÍÊ»ÍÃèÒ á¤¹Ô¹ØèÁã¹à«ÅÇÕâÃèÔµÃºÃÃ¨§ àÇÕÂ§à¨ÃÔ, Ryan O’Handley, Andrew Thompson, »Ò¹à·¾ ÃÑµ¹ÒกÃ

áÅÐ àÂÒÇÅÑกÉ³ì ÊØ¢¸¹Ð………………………………………………………………...….…............. 13¤ÇÒÁªØก¢Í§âÃ¤»ÒกáÅÐà·éÒà»×èÍÂã¹â¤-กÃÐº×Í ·Õè¼èÒ¹ èÒ¹กÑกกÑ¹ÊÑµÇìÃÐËÇèÒ§»ÃÐà·È กÒ¨¹ºØÃÕ ÃÐËÇèÒ§»Õ 2549-2550

ÊÃÂØ·¸ ÊÕ¢ÒÇ áÅÐ ÍÀÔÃ Õ ÍÔ¹·Ã¾ÑกµÃì………………………………….…………..………............. 21กÒÃÈÖกÉÒàº×éÍ§µé¹ : ¤Ø³¤èÒ·Ò§âÀª¹Ð¢Í§àÈÉªÔé¹ÊèÇ¹»ÅÒà¼ÒÐ·ÕèàËÅ×Í¨ÒกกÒÃªÓáËÅÐ

ª¹Ò Ô» ¸ÃÃÁกÒÃ ³Ë·ÑÂ ÇÔ ÔµâÃ·ÑÂ áÅÐ ¨ÃÃÂÒ ¤§Ä· Ôì……………………...………............. 32กÒÃµÃÇ¨ÊÍº¤Ø³ÀÒ¾ éÒ¹ÊØ¢ÈÒÊµÃì¢Í§à¹×éÍÊØกÃáÅÐà¹×éÍâ¤·Õè ÓË¹èÒÂã¹µÅÒ´Ê´áÅÐ«Ø»à»ÍÃìÁÒÃìàกµà¢µ¾×é¹·ÕèÍÓàÀÍàÁ×Í§à¾ªÃºØÃÕ áÅÐÍÓàÀÍËÑÇËÔ¹

ÀÙ Ä· Ôì ÇÔ·ÂÒ¾Ñ²¹Ò¹ØÃÑกÉì ÃÑกÉÒÈÔÃÔ áÅÐ ÃÑªกÄª àÅÔÈÀÑ·ÃâกÁÅ……..………..……...…............. 40

ÃÒÂ§Ò¹ÊÑµÇì»èÇÂ

กÒÃÃÑกÉÒÀÒÇÐµÔ àª×éÍ·ÃÔ»¾Òâ¹â«ÁáººàÃ×éÍÃÑ§ã¹ÊØ¹Ñ¢: ÃÒÂ§Ò¹ÊÑµÇì»èÇÂàÍกÈÔÉ°ì ºÒÃÁÕªÑÂ¸¹Ñ¹µì ÊØ ÒÃÑµ¹ì »ÑµµÒ¹Õ áÅÐ ¹ÔµÂÒ ºØºÒÅ…………….........……............. 51

º·¤ÇÒÁ

Structures, Localizations and Functions of Taste Receptors

Kazumi Taniguchi and Kazuyuki Taniguchi……….………………………..…….….....….............. 61âÃ¤ºÙÅà«ÅâÅ«ÕÊã¹á¾Ð

ÊÁËÁÒÂ ¢ÒÇ¼ÔÇ áÅÐ ¤ÁกÃÔª ¾ÔÁ¾ìÀÑก Õ……………………………..…...……….…..…….......... 70

»ÃÔÈ¹Ò»ÃÔÈ¹Ò-ÊÑµÇì»èÇÂ

°ÔµÒÃÕÂì àÅÒËÃÑªµ¸Ò¹Ô¹·Ãì áÅÐ à¹È Í¹ØÈÑก ÔìàÊ¶ÕÂÃ…..…………………..……….……............. 49What’s your cytological diagnosis?

ËÑÊ Ô¹·Ãì ºØÈÃÕâÃ¨¹ì áÅÐ ·¹§ÈÑก Ôì ÁÐÁÁ…………………………………..…….….……............ 50

7


»·è 4 ©ºÑº·è 1 Á¡ÃÒ¤Á - ÁÔ¶Ø¹ÒÂ¹ 2552

Journal of Mahanakorn Veterinary Medicine

Volume 4 No. 1 January – June 2009ISSN : 1905 � 7571 http://www.vet.mut.ac.th

Contents

Research

Cultivation of Neospora caninum in Vero Cell

Jitbanjong Wiengcharoen, Ryan O’Handley, Andrew Thompson, Parntep Rattanakorn and

Yaowalark Sukthana………………………………………………………….…..….……...…..…….. 13Seroprevalence of Foot-and-Mouth Disease in Cattle and Buffaloes at

the Kanchanaburi Animal Quarantine Station During 2006-2007Sorayut Seekhaow and Apiradee Intarapuk………………………………………………..….…... 21

Preliminary Study: Nutritional Value of By – Product from Pangasius bocourti Processing

Chanathip Thammakarn, Nahatai Vijitrothai and Chunya Kongrith..…..………………..….….. 32Meat Quality Examination of Pork and Beef in Local Fresh Market and Supermarket

in Muang District and Hun Hin District of Phetchaburi Province

Bhutharit Vittayaphattananurak Raksasiri and Rachakris Lertpatarakomol..……………..….…. 40

Case Report

Treatment of Chronic Trypanosomiasis in Dog: A Case Report

Eakkasit Barameechaithanun, Pimsuda Suwannasaeng, Nittaya Boonbal,

Sudarat Pattanee and Sompong Hoisang……………………………….......….…...……….…… 51

Review Article


Kazumi Taniguchi and Kazuyuki Taniguchi…………………………….……………..…….….…… 61Brucellosis in Goats

Sommai Kaopiew and Komkrich Pimpukdee..……………………………………...……….…..….. 70

Q & A

What’s your diagnosis?

Titaree Laoharatchatathanin and Thanate Anusaksathien………………………......……….…… 49What’s your cytological diagnosis?

Hassadin Boonsriroj and Thanongsak Mamom………………………..……..……...…….….…… 50



8

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ÇÒÃÊÒÃ©ºÑº¹ÕéÁÕà¹×éÍËÒÊÒÃÐÊÓ¤Ñ »ÃÐกÍº´éÇÂ ¼Å§Ò¹ÇÔ¨ÑÂàกÕèÂÇกÑº¾ÂÒ¸Ô â¤áÅÐกÃÐº×Í »ÅÒà¼ÒÐ áÅÐ¤Ø³ÀÒ¾¢Í§à¹×éÍÊØกÃáÅÐà¹×éÍâ¤ ÃÒÂ§Ò¹ÊÑµÇì»èÇÂã¹ÊØ¹Ñ¢ º·¤ÇÒÁàกÕèÂÇกÑºâÃ¤á·é§µÔ´µèÍáÅÐàกÕ èÂÇกÑºµèÍÁÃÑºÃÊ¢Í§ÊÑµÇìª¹Ô´µèÒ§æ «Ö è§ä´éÃÑº¤ÇÒÁกÃØ³Òº·¤ÇÒÁ¨ÒกÈÒÊµÃÒ¨ÒÃÂì Kazumi

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1. àÃ×èÍ§·Õè Ð¹ÓÊè§Å§µÕ¾ÔÁ¾ì1.1. §Ò¹ÇÔ¨ÑÂ (Research Papers) à»ç¹§Ò¹¤é¹¤ÇéÒ·´ÅÍ§ ËÃ×Í§Ò¹ÇÔ¨ÑÂ·Ò§ÇÔªÒกÒÃ·Õ è

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ºÃÃ³Ò ÔกÒÃ¾Ô ÒÃ³ÒàËç¹»ÃÐâÂª¹ìµèÍ·Ò§ÇÔªÒกÒÃÊÑµÇá¾·Âì1.3. º·¤ÇÒÁ (Articles) à»ç¹º·¤ÇÒÁËÃ×ÍÂèÍàÍกÊÒÃ·Õèà»ç¹»ÃÐâÂª¹ì áÅÐàกÕ èÂÇ¢éÍ§กÑº

ÇÔªÒกÒÃ·Ò§ÊÑµÇá¾·Âì ÊÑµÇºÒÅ áÅÐÊÒ¢Ò·ÕèàกÕèÂÇ¢éÍ§·ØกÊÒ¢Ò

2. µé¹©ºÑº2.1. µé¹©ºÑº·Õè¨ÐÊè§ÁÒÅ§¾ÔÁ¾ìµéÍ§äÁèà»ç¹àÃ×èÍ§·Õèà¤ÂµÕ¾ÔÁ¾ì ËÃ×ÍกÓÅÑ§ÍÂÙèÃÐËÇèÒ§¾Ô¨ÒÃ³Ò

à¾×èÍÅ§ã¹Ë¹Ñ§Ê×ÍËÃ×ÍÇÒÃÊÒÃÍ×è¹2.2. µé¹©ºÑº¾ÔÁ¾ìà»ç¹ÀÒÉÒä·Â ËÃ×ÍÀÒÉÒÍÑ§กÄÉ ¨Ó¹Ç¹ 3 ªØ´ ´éÇÂÍÑกÉÃ Browallia

new 16 º¹กÃÐ´ÒÉ¢ÒÇàÍ 4 â´Â¾ÔÁ¾ìË¹éÒà´ÕÂÇ àÇé¹¢ÍºกÃÐ´ÒÉ´éÒ¹«éÒÂ áÅÐ´éÒ¹º¹ 1.5 ¹ÔéÇËÃ×Í 3.81 à«¹µÔàÁµÃ ´éÒ¹¢ÇÒÅèÒ§ áÅÐ´éÒ¹ÅèÒ§ 1 ¹Ô éÇ ËÃ×Í 2.54 à«¹µÔàÁµÃ â´Â¤ÇÒÁÂÒÇ¢Í§àÃ×èÍ§¾ÃéÍÁµÒÃÒ§áÅÐÀÒ¾»ÃÐกÍºÃÇÁáÅéÇäÁèàกÔ¹ 12 Ë¹éÒ áÅÐãËéãÊèËÁÒÂàÅ¢Ë¹éÒµÒÁÅÓ Ñº

2.3. àÁ× èÍä´éÃ ÑºกÒÃáกéä¢áÅÐ¾Ô¨ÒÃ³ÒãËéÅ§µÕ¾ÔÁ¾ìáÅéÇ ¨Ö§¨Ñ´Êè§µé¹©ºÑºÍÔàÅçก·ÃÍ¹ÔกÊì(Electronic file) º¹á¼è¹«Õ´Õ¢éÍÁÙÅ (PC formatted CD) â´Â¾ÔÁ¾ì´éÇÂâ»ÃáกÃÁ Microsoft Word

(Window 98 ËÃ×ÍÊÙ§กÇèÒ) ¾ÃéÍÁÊÓà¹Ò·Õè¾ÔÁ¾ìÅ§º¹กÃÐ´ÒÉ¢ÒÇàÍ 4 ¨Ó¹Ç¹ 2 ªØ2.4. äÁèÁÕกÒÃÊè§¤×¹µé¹©ºÑº ã¹กÃ³Õ·ÕèäÁè¼èÒ¹กÒÃ¾Ô ÒÃ³Òáµè Ðá é§ãËé·ÃÒº

3. กÒÃÅÓ ÑºàÃ×èÍ§¤ÇÃàÃÕÂ§ Ñ§¹Õé3.1. ª×èÍàÃ×èÍ§ (Title) ¤ÇÃµÑé§ª×èÍãËéÊÍ´¤ÅéÍ§ Ê×èÍ¤ÇÒÁËÁÒÂä é ÕกÑºà¹×éÍËÒã¹àÃ×èÍ§



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3.2. ª× èÍ¼Ù éà¢ ÕÂ¹áÅÐ¼Ù éÃ èÇÁà¢ÕÂ¹ (Author and Co-authors) à¢ÕÂ¹ª× èÍ¹ÒÁÊกØÅàµçÁ·Ñ é§ÀÒÉÒä·ÂáÅÐÀÒÉÒÍÑ§กÄÉãµéª× èÍàÃ× èÍ§ ¾ÃéÍÁ·Ñ é§Ê¶Ò¹·Õ è·Ó§Ò¹·Õ èµÔ´µèÍä´éÊÐ´Çก áÅÐกÃØ³ÒºÍกËÁÒÂàÅ¢â·ÃÈÑ¾·ìËÃ×Íâ·ÃÊÒÃ áÅÐ E-mail ¢Í§¼ÙéÃÑº¼Ô´ªÍº (Corresponding author) à¾×èÍ¤ÇÒÁÃÇ´àÃçÇã¹กÒÃµÔ µèÍ â´ÂãËéÃÐºØÍÂÙèãµé¤ÓÊÓ¤Ñ

3.3. º·¤Ñ´ÂèÍ (Abstract) à¢ÕÂ¹ÊÑé¹æ ãËéä´éà¹×éÍ¤ÇÒÁ¤ÃÍº¤ÅØÁ·Ñé§ËÁ´ ã¹กÃ³Õ·Õèµé¹©ºÑºÀÒÉÒä·Â¨ÐµéÍ§ÁÕª×èÍàÃ×èÍ§áÅÐº·¤Ñ´ÂèÍà»ç¹ÀÒÉÒÍÑ§กÄÉ áÅÐµé¹©ºÑºÀÒÉÒÍÑ§กÄÉµéÍ§ÁÕª×èÍàÃ×èÍ§áÅÐº·¤Ñ ÂèÍà»ç¹ÀÒÉÒä·Â º·¤Ñ ÂèÍãËéà¢ÕÂ¹äÇéË¹éÒÊØ ·éÒÂ¢Í§àÃ×èÍ§à»ç¹Ë¹éÒË¹Öè§µèÒ§ËÒก

3.4. ¤ÓÊÓ¤Ñ (Keywords) à»ç¹¤ÓËÃ×Í¢éÍ¤ÇÒÁÊÑé¹æ ·ÕèÁÕ¤ÇÒÁËÁÒÂáÊ´§¶Ö§¤ÇÒÁà»ç¹ä»¢Í§กÒÃ·´ÅÍ§¹Ñé¹æ ÃÇÁกÑ¹áÅéÇäÁèàกÔ¹ 5 ¤Ó ÃÐºØÍÂÙèãµéº·¤Ñ´ÂèÍ (¢Öé¹ºÃÃ·Ñ´ãËÁè) ·Ñé§ÀÒÉÒä·ÂáÅÐÀÒÉÒÍÑ§กÄÉ

3.5. º·¹Ó (Introduction) ºÃÃÂÒÂ¤ÇÒÁà»ç¹ÁÒ áÅÐ¤ÇÃÁÕกÒÃµÃÇ¨àÍกÊÒÃ (Literature

review) »ÃÐกÍº éÇÂ ÃÇÁ·Ñé§Í ÔºÒÂ¶Ö§ Ø »ÃÐÊ§¤ì¢Í§กÒÃ·´ÅÍ§3.6. ÍØ»กÃ³ìáÅÐÇÔ¸ÕกÒÃ (Materials and Methods) ã¹กÃ³Õ·Õèà»ç¹กÒÃ¤Ô´¤é¹¢Öé¹ãËÁ¤ÇÃ

Í¸ÔºÒÂÍÂèÒ§ÅÐàÍÕÂ´ ¶éÒà»ç¹ÇÔ¸ÕกÒÃ·Õè·ÃÒºกÑ¹ÍÂÙèáÅéÇáÅÐà¤ÂÁÕ¼ÙéµÕ¾ÔÁ¾ìáÅéÇ äÁèµéÍ§ºÃÃÂÒÂ«éÓ¤ÇÃà¢ÕÂ¹ã¹ÅÑกÉ³ÐÍéÒ§ÍÔ§ áÅÐÍ¸ÔºÒÂà©¾ÒÐÊèÇ¹·Õè´Ñ´á»Å§ËÃ×Íà¾ÔèÁàµÔÁ ¾ÃéÍÁ·Ñé§ÇÔ¸ÕÇÔà¤ÃÒÐËì¼ÅกÒÃ·´ÅÍ§·Ò§Ê¶ÔµÔ éÇÂ

3.7. ¼ÅกÒÃ·´ÅÍ§ (Results) กÒÃÃÒÂ§Ò¹¼ÅกÒÃ·´ÅÍ§à»ç¹¤ÓºÃÃÂÒÂ¤ÇÃÅÐàÍÕÂ´áÅÐà¢éÒã¨§èÒÂ ËÒกà»ç¹ä»ä´é¤ÇÃàÊ¹Í¼Åã¹ÃÙ»µÒÃÒ§ ÃÙ»ÀÒ¾ ËÃ×ÍกÃÒ¿ äÁè¤ÇÃáÊ´§¼Å·ÕèàËÁ×Í¹กÑ¹ ¶éÒà»ç¹µÒÃÒ§¤ÇÃãËéªÑ´à¨¹áÅÐ¢¹Ò´¾ÍàËÁÒÐกÑº¢¹Ò´¢Í§Ë¹éÒË¹Ñ§Ê×Í µÒÃÒ§¤ÇÃÁÕ¤ÇÒÁËÁÒÂã¹µÑÇàÍ§áÅÐµéÍ§ÁÕ¤ÓÍ¸ÔºÒÂàË¹×ÍµÒÃÒ§´éÇÂ ã¹กÃ³Õ·Õèà»ç¹ÃÙ»ÀÒ¾ (Figures) ¤ÇÃà»ç¹ÀÒ¾¢ÒÇ´ÓËÃ×ÍÊäÅ´ìËÒกµéÍ§กÒÃãËéµÕ¾ÔÁ¾ìÀÒ¾ÊÕ ·Ò§¤³Ð¼Ùé¨Ñ´·Ó¨Ð¾Ô¨ÒÃ³Ò¶Ö§¤ÇÒÁàËÁÒÐÊÁáÅÐ¤èÒãªé¨èÒÂ ËÒกÁÕËÅÒÂÃÙ»µéÍ§àÃÕÂ§ÅÓ´ÑºกèÍ¹ËÅÑ§¾ÃéÍÁ·Ñé§ÁÕà¤Ã×èÍ§ËÁÒÂกÓË¹´ºÍก´éÒ¹º¹¢Í§ÃÙ»´éÇÂ´Ô¹ÊÍ áÅÐÍ¸ÔºÒÂÃÒÂÅÐàÍÕÂ´äÇéãµéÃÙ»

3.8. ÇÔ¨ÒÃ³ì (Discussion) à»ç¹กÒÃÇÔ¨ÒÃ³ì¼ÅกÒÃ·´ÅÍ§ กÒÃ»ÃÐàÁÔ¹¼Å áÅÐกÒÃµÕ¤èÒ¢Í§¼Å§Ò¹ กÒÃÇÔ¨ÒÃ³ì¼Å¤ÇÃà»ÃÕÂºà·ÕÂºกÑº¼Å§Ò¹¢Í§¼ÙéÍ× è¹·Õèä´éกÃÐ·ÓÁÒáÅéÇ áÅÐ¤ÇÃà¹é¹¶Ö§ÊÔè§·Õèä´é¤é¹¾º

3.9. ÊÃØ» (Conclusion) ÍÒ¨ÁÕËÃ×ÍäÁèกçä´é ËÒกà»ç¹º·¤ÇÒÁ กÒÃµÃÇ¨àÍกÊÒÃ ËÃ×Íà»ç¹กÒÃ·´ÅÍ§·ÕèÁÕËÅÒÂ¢éÍ ¤ÇÃÁÕº·ÊÃØ»·Õèà¢ÕÂ¹ã¨¤ÇÒÁÊÓ¤ÑáÅÐ¤Ø³¤èÒ¢Í§§Ò¹à¾×èÍ¼ÙéÍèÒ¹¨Ðä éà¢éÒã¨ÁÒก¢Öé¹

3.10.กÔµµÔกÃÃÁ»ÃÐกÒÈ (Acknowledgement) ¤ÇÃÁÕã¹กÃ³Õ·Õ èä´éÃÑº¤ÇÒÁªèÇÂàËÅ×ÍËÃ×Í¤ÇÒÁÃèÇÁÁ×Íã¹กÒÃÊ¹ÑºÊ¹Ø¹§Ò¹¤é¹£ÇéÒÇÔ ÑÂ

3.11. àÍกÊÒÃÍéÒ§ÍÔ§ (References)

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4. กÒÃà¢ÕÂ¹ÍéÒ§ÍÔ§ã¹à¹×éÍàÃ×èÍ§¤ÇÃÍéÒ§ÍÔ§ Ñ§¹Õé- กÃ³ÕÍéÒ§ÍÔ§¨ÒกกÒÃµÃÇ¨àÍกÊÒÃâ´Â¼ÙéÍ×è¹ãËéãªé ¤ÓÇèÒ ÍéÒ§¶Ö§â´Â (Cited by)- ã¹กÃ³Õ·Õè¼ÙéÃÒÂ§Ò¹àÍกÊÒÃà»ç¹¤¹ä·Â àÁ×èÍà»ç¹»ÃÐ¸Ò¹¢Í§»ÃÐâÂ¤ãËéãªé ÊÁªÒÂ (2549)

ËÃ×ÍàÁ×èÍ¼ÙéÃÒÂ§Ò¹ÍÂÙèกÅÒ§ËÃ×Í·éÒÂ»ÃÐâÂ¤ àªè¹ (ÊÁÈÃÕ, 2549), (ÊÁÈÑก Ôì áÅÐ¤³Ð, 2549)- ã¹กÃ³Õ·Õ è¼Ù éÃÒÂ§Ò¹àÍกÊÒÃà»ç¹ªÒÇµèÒ§»ÃÐà·È àÁ× èÍà»ç¹»ÃÐ¸Ò¹¢Í§»ÃÐâÂ¤ãËéãªé

Tomato and Danny (2006), Taylor et al. (2006) ËÃ×ÍàÁ×èÍ¼ÙéÃÒÂ§Ò¹ÍÂÙèกÅÒ§áÅÐ·éÒÂ»ÃÐâÂ¤ãËéãªé(Tomato and Danny, 2006), (Taylor et al., 2006)

- ÍéÒ§¶Ö§ºØ¤¤Å ËÃ×ÍàÃ×èÍ§·ÕèäÁèà¤ÂµÕ¾ÔÁ¾ìÁÒกèÍ¹ (Personal comm.) ãËéÍéÒ§à©¾ÒÐã¹à¹×éÍàÃ×èÍ§à·èÒ¹Ñé¹ äÁèµéÍ§¹Óä»Å§ã¹ÃÒÂª×èÍàÍกÊÒÃÍéÒ§ÍÔ§

- กÒÃà¢ÕÂ¹àÍกÊÒÃÍéÒ§ÍÔ§·éÒÂàÃ × èÍ§¤ÇÃÍéÒ§ÍÔ§àÍกÊÒÃÀÒÉÒä·ÂกèÍ¹áÅéÇµÒÁ´éÇÂÀÒÉÒÍÑ§กÄÉ â´Âà¢ÕÂ¹àÃÕÂ§ÅÓ´Ñº¾ÂÑª¹Ð ¢Í§ª×èÍ¼Ùéà¢ÕÂ¹ (¶éÒà»ç¹ÀÒÉÒÍÑ§กÄÉãªéª×èÍÊกØÅ µÒÁ´éÇÂª×èÍÂèÍ¢Í§¼Ùéáµè§) µÒÁ´éÇÂ »Õ ª×èÍàÃ×èÍ§ ª×èÍË¹Ñ§Ê×Í ËÃ×Íª×èÍÂèÍÇÒÃÊÒÃ (¾ÔÁ¾ìµÑÇàÍ¹) »Õ·Õè ©ºÑº·Õè áÅÐË¹éÒ·ÕèÍéÒ§ÍÔ§ Ñ§µÑÇÍÂèÒ§ ¤×Í

àกè§ Õ àªÕèÂÇªÒ Õ àกè§ÁÒก àªÕèÂÇªÒÁÒก áÅÐ àกè§·ÕèÊØ àªÕÂÇªÒ·ÕèÊØ . 2552. กÒÃÈÖกÉÒà»ÃÕÂºà·ÕÂº¤ÇÒÁàกè§¢Í§¼ÙéÇÔ ÑÂ. ÇÒÃÊÒÃÇÔªÒกÒÃ. 18(3) : 22-30.

Excellenta, S.A., Excellente, S.B. and Excellento, S.C. 2009. Researcher performances for publishing

in a journal. J Excellence Eaa. 78 : 215-222.

- ËÒกàÍกÊÒÃÍéÒ§ÍÔ§à»ç¹µÓÃÒ ãËéÃÐºØª×èÍ¼Ùéà¢ÕÂ¹ »Õ·Õè¾ÔÁ¾ì ª×èÍàÃ×èÍ§ ª×èÍµÓÃÒ ¤ÃÑé§·Õè¾ÔÁ¾ìáÅÐª×èÍºÃÃ³Ò ÔกÒÃ (ËÒกÁÕ) ÊÓ¹Ñก¾ÔÁ¾ì àÁ×Í§·Õè¾ÔÁ¾ì áÅÐ¨Ó¹Ç¹Ë¹éÒ·Ñé§ËÁ´ Ñ§µÑÇÍÂèÒ§ ¤×Í

àกè§ÁÒก ÃÑกกÒÃÍèÒ¹. 2552. กÒÃÍèÒ¹กÑº¤ÇÒÁàกè§. ÊÓ¹Ñก¾ÔÁ¾ìกÒÃÍèÒ¹ Õ. กÃØ§à·¾ÁËÒ¹¤Ã. 125 Ë¹éÒExcella, H.A. 2009. Expertization by reading. 4th edition. Angthong. A.B.C. publishing. Philad. 430 p.



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ºÃÃ³Ò ÔกÒÃ ÊÑµÇá¾·ÂìÁËÒ¹¤ÃÊÒÃ¤³ÐÊÑµÇá¾·ÂÈÒÊµÃì ÁËÒÇÔ·ÂÒÅÑÂà·¤â¹âÅÂÕÁËÒ¹¤Ã140 ¶. àª×èÍÁÊÑÁ¾Ñ¹ ì à¢µË¹Í§¨ÍกกÃØ§à·¾ÁËÒ¹¤Ã 10530â·Ã. 0-2988-3655 µèÍ 5102-3â·ÃÊÒÃ 0-2988-4040

ËÁÒÂàËµØ1. ª×èÍ·Ò§ÇÔ·ÂÒÈÒÊµÃì·Ñé§ÀÒÉÒÍÑ§กÄÉáÅÐ·ÑºÈÑ¾·ìÀÒÉÒä·ÂãËé¾ÔÁ¾ìâ´ÂãªéµÑÇÍÑกÉÃàÍÕÂ§2. äÁèÁÕ¤èÒàÃ×èÍ§Å§¾ÔÁ¾ìáÅÐ¼Ùéà¢ÕÂ¹µéÍ§ÃÑº¼Ô´ªÍº¤èÒ¾ÔÁ¾ìÀÒ¾ÊÕ ËÒกà»ç¹¤ÇÒÁµéÍ§กÒÃ¢Í§¼Ùéà¢ÕÂ¹

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2 incubator

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1¤³ÐÊÑµÇá¾·ÂÈÒÊµÃì ÁËÒÇÔ·ÂÒÅÑÂà·¤â¹âÅÂÕÁËÒ¹¤Ã2Division of Veterinary and Biomedical Sciences, Murdoch University, Western Australia3¤³ÐÊÑµÇá¾·ÂÈÒÊµÃì ÁËÒÇÔ·ÂÒÅÑÂÁËÔ Å4¤³ÐàÇªÈÒÊµÃìà¢µÃéÍ¹ ÁËÒÇÔ·ÂÒÅÑÂÁËÔ Å#¼ÙéÃÑº¼Ô ªÍºº·¤ÇÒÁ / E-mail : [email protected]


Vol. 4 No. 1 ☺anuar - ☺une 2009

14

¹ÕâÍÊ»ÍÃèÒ á¤¹Ô¹Ø èÁ (Neospora caninum) à»ç¹àª× éÍâ»Ãâµ«ÑÇ·Õ èµ éÍ§ÍÒÈÑÂà»ç¹»ÒÃÒÊÔµÍÂÙ èÀÒÂã¹à«ÅÅì¢Í§âÎÊµì (obligate intracellular parasite) àª× éÍ¹ÕâÍÊ»ÍÃèÒ¶ÙกÃÒÂ§Ò¹¶Ö§กÒÃกèÍâÃ¤¤ÃÑ é§áÃกã¹»Õ ¤.È.1984 ·Õ è»ÃÐà·È¹ÍÃàÇÂìâ´ÂกèÍâÃ¤ÊÁÍ§áÅÐä¢ÊÑ¹ËÅÑ§ÍÑกàÊºã¹ÊØ¹Ñ¢(encephalomyelitis) (Bjerkas et al., 1984) áµèÁÑ¹¶Ùกà¢éÒã¨¼Ô´áÅÐÃÒÂ§Ò¹ÇèÒà»ç¹àª× éÍ·çÍกâ«¾ÅÒÊÁÒ กÍ¹ä´ (Toxoplasma gondii) à¹× èÍ§¨Òกàª× éÍ·Ñ é§ÊÍ§ª¹Ô´¹Õ é¨ÐÁÕÃ Ù»ÃèÒ§ÅÑกÉ³Ð·Õ è¤ÅéÒÂกÑ¹ÁÒก¨¹áÂกáÂÐäÁèä´é´éÇÂกÅéÍ§¨ØÅ·ÃÃÈ¹ì¸ÃÃÁ´Ò µèÍÁÒã¹»Õ ¤.È.1988 Dubey ¨Ö§ä´éÃÒÂ§Ò¹ÇèÒÁÑ¹à»ç¹àª×éÍ¤¹ÅÐª¹Ô กÑ¹áÅÐä éµÑé§ª×èÍãËéãËÁè éÇÂ (Dubey et al., 1988)

»Ñ¨¨ØºÑ¹¹Õéàª×éÍ¹ÕâÍÊ»ÍÃèÒà»ç¹·ÕèÃÙ é¨ÑกกÑ¹´Õ·ÑèÇâÅกã¹°Ò¹Ð·ÕèกèÍâÃ¤¹ÕâÍÊ»ÍâÃ«ÔÊ (neosporosis)«Ö è§à»ç¹Ë¹Ö è§ã¹ÊÒàËµØËÅÑก¢Í§กÒÃá·é§ (abortion) ã¹â¤·Ñ é§â¤à¹× éÍáÅÐâ¤¹Á·Ñ èÇâÅก¹Íก¨Òกàª× éÍ¹ÕâÍÊ»ÍÃèÒ¨ÐÊÒÁÒÃ¶·ÓãËéàก Ô´กÒÃá·é§ã¹â¤ä´éáÅéÇ ÁÑ¹ÂÑ§ÍÒ¨·ÓãËéàก Ô´กÒÃµÒÂáÃก¤ÅÍ´¢Í§ÅÙกâ¤àกÔ´ãËÁè (stillbirth), กÒÃµÔ´àª× éÍµÑ é§áµèáÃกàกÔ´ã¹ÅÙกâ¤ (congenital

neosporosis), กÒÃ¼ÅÔµ¹éÓ¹Á¢Í§áÁèâ¤Å´Å§ (reduce milk production) áÅÐÍÑµÃÒกÒÃ¶Ùก¤Ñ´·Ôé§¢Í§â¤ÊÙ§¢Ö é¹ (increase culling) ä´éÍÕก´éÇÂ (Anderson et al., 2000; Gottstein et al., 2000;Dubey et al., 2006; Dubey et al., 2007) à¹×èÍ§¨Òกã¹»Ñ¨¨ØºÑ¹ÂÑ§äÁèÁÕÂÒµÑÇã´·ÕèÁÕ»ÃÐÊÔ·¸ÔÀÒ¾´Õ¾Í·Õ è¨ÐãªéÃÑกÉÒâ¤·Õ èµÔ´âÃ¤¹ÕâÍÊ»ÍâÃ«ÔÊãËéËÒÂä´é กÒÃ¨Ñ´กÒÃÊÓËÃÑºâ¤·Õ èµÔ´àª× éÍáÅéÇกç¤×ÍµéÍ§¤Ñ ·Ôé§à¾×èÍäÁèãËéàกÔ กÒÃá¾ÃèกÃÐ¨ÒÂ¢Í§àª×éÍÀÒÂã¹½Ù§à¾ÔèÁÁÒก¢Öé¹

ÊÓËÃÑºã¹»ÃÐà·Èä·Â ÁÕÃÒÂ§Ò¹กÒÃÊÓÃÇ¨¾ºกÒÃµÔ´àª× éÍ¹ÕâÍÊ»ÍÃèÒã¹â¤áÅÐÊØ¹Ñ¢â´ÂKyaw et al. (2004) ÃÒÂ§Ò¹ÇèÒÁÕâ¤·Õèà»ç¹ seropositive 30 µÑÇ (5.5%) ¨Òกâ¤·Ñé§ËÁ´ 549 µÑÇ ã¹¨Ñ§ËÇÑ´¹¤Ã»°Áâ´ÂãªéกÒÃÇÔ¹ Ô¨©ÑÂâ´Â competitive ELISA (cELISA) áÅÐÁÕÊ Ø¹ Ñ¢·Õ è à» ç¹seropositive 1 µÑÇ (1.2%) ¨ÒกÊØ¹Ñ¢·Ñé§ËÁ´ 82 µÑÇ, Suteeraparp et al. (1999) ÃÒÂ§Ò¹ÇèÒÁÕâ¤·Õèà»ç¹ seropositive 6% ¨Òกâ¤¹Á·Ñé§ËÁ´ 904 µÑÇ ¨Òก 11 ¨Ñ§ËÇÑ´ã¹ÀÒ¤กÅÒ§¢Í§ä·ÂÇÔ¹Ô¨©ÑÂâ´Âãªéà·¤¹Ô¤ Indirect Immunofluorescence Antibody Test (IFAT) áÅÐ Kashiwazaki et al.,(2001) ÃÒÂ§Ò¹¤é¹¾º¨Ó¹Ç¹â¤·Õèà»ç¹ seropositive à¾ÔèÁ¢Öé¹¨Òก 37.5 à»ç¹ 62.5% ã¹â¤¹Á 83µÑÇ µÑé§áµèªèÇ§à ×Í¹ÁÔ¶Ø¹ÒÂ¹¨¹¶Ö§à ×Í¹¾ÄÈ ÔกÒÂ¹â´Âãªéà·¤¹Ô¤ IFAT

Ç§ªÕÇ Ôµ¢Í§àª× éÍ¹ÕâÍÊ»ÍÃèÒ»ÃÐกÍº´éÇÂ 3 ÃÐÂÐ ¤×Í tachyzoite, tissue cyst áÅÐoocyst (ÃÙ»·Õ è1) ÃÐÂÐ tachyzoite áÅÐ tissue cyst ¨Ð¾ºä´éã¹âÎÊµìกÖ è§กÅÒ§ä´éáกè â¤ กÃÐº×Íá¾Ð áกÐ กÇÒ§ ÃÐÂÐ tachyzoite ¨ÐÁÕÃ Ù»ÃèÒ§¤ÅéÒÂกÅéÇÂËÍÁËÃ×Í¾ÃÐ¨Ñ¹·ÃìàÊ Õ éÂÇ ÁÕ¢¹Ò´»ÃÐÁÒ³ 6 x 2 äÁ¤ÃÍ¹ tissue cyst ÁÑกÁÕÃÙ»ÃèÒ§กÅÁËÃ×ÍÃÙ»ä¢è (round or oval) â´ÂÁÕ¢¹Ò´¤ÇÒÁÂÒÇä´é¶Ö§ 107 äÁ¤ÃÍ¹ÁÑก¾ºä´éã¹ÃÐºº CNS áÅÐกÅéÒÁà¹× éÍÅÒÂ ¼¹Ñ§¢Í§«ÕÊµìË¹Òä´é¶ Ö§ 4 äÁ¤ÃÍ¹ÀÒÂã¹«ÕÊµì¨ÐºÃÃ¨Ø bradyzoite ¢¹Ò´ 7-8 x 2 äÁ¤ÃÍ¹¨Ó¹Ç¹ÁÒกàÍÒäÇébradyzoite ¨ÐÁÕÃÙ»ÃèÒ§¤ÅéÒÂกÅéÇÂËÍÁàËÁ×Í¹กÑ¹กÑºÃÐÂÐ tachyzoite (Buxton et al., 2002;Dubey, 2003) ÊèÇ¹ÃÐÂÐ oocyst ¹Ñ é¹¨Ð¶ÙกÊÃéÒ§¢Ö é¹ä´éÀÒÂã¹¼¹Ñ§ÅÓäÊé¢Í§âÎÊµìÊØ´·éÒÂä´éáกèÊ Ø¹ Ñ¢ºéÒ¹áÅÐ coyote áÅéÇ¨Ð¶Ùก¢ÑºÍÍกÁÒ»¹กÑºÍØ¨¨ÒÃÐ¢Í§ÊØ¹Ñ¢ oocyst ¨ÐÁÕ¼¹Ñ§¤èÍ¹¢éÒ§

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Ë¹Ò ÃÙ»ÃèÒ§กÅÁ ¢¹Ò´ 11.7 x 11.3 äÁ¤ÃÍ¹áÅÐÁÑ¹¨Ðà»ç¹ÃÐÂÐ·ÕèÁÕ¤ÇÒÁ·¹·Ò¹ã¹ÊÔè§áÇ´ÅéÍÁ(McAllister et al., 1998; Lindsay et al., 1999; Bergeron et al., 2001; Buxton et al.,2002) ·Ñ é§ 3 ÃÐÂÐ¢Í§àª× éÍ¹ÕâÍÊ»ÍÃèÒÊÒÁÒÃ¶à»ç¹ÃÐÂÐµÔ´µèÍ (infective stage) ä´éàªè¹ÊØ¹Ñ¢ÍÒ¨¨ÐµÔ´àª× éÍä´éâ´ÂกÒÃกÔ¹à¹× éÍ´Ôºæ·Õ èÁÕàª× éÍ¹ÕâÍÊ»ÍÃèÒÃÐÂÐ cyst ËÃ×Í bradyzoite à¢éÒä» â¤ÍÒ¨¨ÐµÔ´àª× éÍä´éâ´ÂกÒÃกÔ¹ÍÒËÒÃËÃ×Í¹éÓ·Õ èÁÕàª× éÍ¹ÕâÍÊ»ÍÃèÒÃÐÂÐ oocyst »¹à»× éÍ¹à¢éÒä» ËÃ×ÍÅÙกâ¤ÍÒ¨¨ÐµÔ´âÃ¤¨ÒกáÁèâ¤ä´éµÑ é§áµèÂÑ§ÍÂÙ èã¹·éÍ§áÁèâ´ÂกÒÃà¤Å× èÍ¹·Õ è¢Í§àª× éÍÃÐÂÐ tachyzoite

¼èÒ¹·Ò§Ãก¨ÒกáÁèä»ÊÙ èÅ Ùก (transplacental transmission) «Ö è§Ç Ô¸ ÕกÒÃ¶èÒÂ·Í´àª× éÍáººtransplacental transmission ¹Õéà»ç¹ÇÔ ÕËÅÑก¢Í§กÒÃá¾ÃèกÃÐ¨ÒÂ¢Í§âÃ¤¹ÕâÍÊ»ÍâÃ«ÔÊ

Ç§ªÕÇÔµ¢Í§ Neospora caninum (Dubey, 2003).

ÍØ»กÃ³ìáÅÐÇÔ ÕกÒÃàª× éÍ N.caninum strain K9WA ÃÐÂÐ tachyzoite ã¹ vero cell ä´éÃÑºÁÒà»ç¹¤ÃÑ é§áÃก¨Òก

The Animal Health Laboratories, Agriculture Department, Western Australia àª× éÍ¶Ùก¹ÓÁÒàÅÕ éÂ§µèÍã¹µÙ é humidified incubator ·Õ è 37°C, 5%CO

2 ·Õ èÀÒ¤ÇÔªÒ¾ÂÒ¸Ôâ»Ãâµ«ÑÇ ¤³Ð

àÇªÈÒÊµÃìà¢µÃéÍ¹ ÁËÒÇÔ·ÂÒÅÑÂÁËÔ ÅµèÍÁÒãªéกÅéÍ§ Inverted microscope µÃÇ¨´Ù·ØกÇÑ¹ÇÑ¹ÅÐ 2 ¤ÃÑ é§àÁ× èÍ¾ºÇèÒ vero cell ÁÕ

กÒÃ infected ´éÇÂ N.caninum ÍÂèÒ§Ë¹Ñก áÅÐ¾º tachyzoite ·Õ èÍÂÙ èà»ç¹ÍÔÊÃÐÍÂÙ èÀÒÂ¹Íกà«ÅÅìâ´Âà©¾ÒÐã¹ºÃÔàÇ³·Õ è monolayer ¶Ùก·ÓÅÒÂËÃ×ÍáµกàÊÕÂËÒÂ (ÃÙ»·Õ è 2) ãªé cell scraper ¢Ù´monolayer ÍÍก¨Òก¼ÔÇ¢Í§ flask áÅéÇ¹Ó medium ·Õ èÁÕ tachyzoite ¢Í§ N.caninum ÍÍกÁÒ¹Ñº¨Ó¹Ç¹º¹ hemocytometer กèÍ¹·Õ è¨Ð inoculate àª× éÍ N.caninum ¨Ó¹Ç¹ 105 tachyzoites Å§ä»º¹ vero cell ·Õèà»ç¹ monolayer ¨Òก¹Ñé¹¹Ó flask ä»Íºã¹µÙé humidified incubator ·Õè 37°C,5%CO

2 (ÃÙ»·Õè 3)ËÅÑ§¨Òก¹Ñé¹·Ó subculture ÍÕกàÁ×èÍ¾ºÇèÒ vero cell µÔ àª×éÍÍÂèÒ§Ë¹Ñก

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กÒÃàµÃÕÂÁÍÒËÒÃàÅÕéÂ§àª×éÍÍÒËÒÃàÅÕ éÂ§àª× éÍ¨Ðãªé DMEM/HIGH w/o L-Glutamine (Hyclone) à»ç¹ËÅÑกáÅéÇàµÃÕÂÁ

ÍÒËÒÃ 2 ÊÙµÃ¤×Í ÊÙµÃ Growth culture medium « Ö è§ãª é fetal calf serum 10% áÅÐÊÙµÃMaintenance culture medium ãªé fetal calf serum 2% ÍÒËÒÃàÅÕ éÂ§àª× éÍÊÙµÃáÃก¨ÐãªéÊÓËÃÑºกÒÃà¾ÒÐàÅÕ éÂ§áÅÐà¾Ô èÁ¨Ó¹Ç¹ vero cell ã¹ªèÇ§áÃกกèÍ¹·Õ è¨Ð inoculate àª× éÍ¹ÕâÍÊ»ÍÃèÒÅ§ä»ÊèÇ¹ÍÒËÒÃÍÕกÊÙµÃË¹Öè§¨ÐãªéËÅÑ§¨Òก·Õè inoculate àª×éÍ¹ÕâÍÊ»ÍÃèÒÅ§ä»ã¹ vero cell áÅéÇ

àª × éÍ N.caninum strain K9WA ÃÐÂÐ tachyzoite ÁÕกÒÃà¨ÃÔàµÔºâµ¤èÍ¹¢éÒ§ªéÒã¹ªèÇ§áÃกæ·Õ èàÃ Ô èÁà¾ÒÐàÅÕ éÂ§ à¹× èÍ§¨ÒกÁÕกÒÃà»ÅÕ èÂ¹á»Å§ÊÀÒ¾áÇ´ÅéÍÁËÅÒÂÍÂèÒ§ÃÇÁ·Ñ é§Ê ÙµÃÍÒËÒÃ·Õ èãªéà¾ÒÐàÅÕ éÂ§กçäÁèàËÁ×Í¹à´ÔÁ ËÅÑ§¨Òก¼èÒ¹ä»»ÃÐÁÒ³ 3-4 passages àª× éÍàÃÔ èÁ»ÃÑºµÑÇä é Õ¢Öé¹áÅÐà¨ÃÔàµÔºâµä éÃÇ´àÃçÇ ÕÁÒก ËÅÑ§¨Òก¹Ñé¹àª×éÍ¨Ð¶Ùก subculture ·Øกæ 4-5 ÇÑ¹

ÀÒ¾áÊ´§ t issue culture · Õ èÁ Õàª × éÍ N.caninum àÁ × èÍ¹Óä»ÊèÍ§´Ù´ éÇÂกÅéÍ§ Inverted

microscope ¨Ð¾ºÁÕàª × éÍÃÐÂÐ tachyzoite ¨Ó¹Ç¹ÁÒกÍÂÙ èÀÒÂã¹à«ÅÅì vero áÅÐÍÒ¨¾ººÒ§tachyzoite ·Õèà»ç¹ÍÔÊÃÐÍÂÙèã¹ºÃÔàÇ³·Õè vero cell ¶Ùก·ÓÅÒÂä»áÅéÇ (ÅÙกÈÃªÕé)

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¢Ç´ tissue culture flask ·ÕèãªéÊÓËÃÑºà¾ÒÐàÅÕéÂ§àª×éÍ N.caninum ÀÒÂã¹à«ÅÅìÇÕâÃèáÅÐÍÒËÒÃàÅÕéÂ§àª×éÍ

DMEM áÅÐµÙé humidified incubator

à¹× èÍ§¨ÒกÁÕÃÒÂ§Ò¹กÒÃกèÍâÃ¤¢Í§àª× éÍ¹ÕâÍÊ»ÍÃèÒã¹â¤àกÔ´¢Ö é¹·Ñ èÇâÅกÃÇÁ·Ñ é§ã¹»ÃÐà·Èä·Â´éÇÂáµèÇ èÒÇ§ªÕÇ Ôµ ªÕÇÇÔ·ÂÒ áÅÐกÅäกกÒÃกèÍâÃ¤¢Í§ÁÑ¹กÅÑºÂÑ§äÁèà»ç¹·Õ èà¢ éÒã¨กÑ¹´Õ¹Ñก กÒÃà¾ÒÐàÅ Õ éÂ§ËÃ ×ÍกÒÃ¼Å Ôµàª × éÍ¹ Õ âÍÊ»ÍÃ èÒã¹Ë éÍ§·´ÅÍ§ä´ é à» ç¹¼ÅÊÓàÃ ç¨¨ÐÊÒÁÒÃ¶¹Óä»ãª é»ÃÐâÂª¹ìä´éÁÒกàªè¹àÃÒÍÒ¨¨Ð¹ÓàÍÒàª× éÍ·Õ èàÅ Õ éÂ§ä´éä»ãªéã¹กÒÃÊกÑ´áÍ¹µÔà¨¹ËÃ×ÍÊกÑ´´ÕàÍç¹àÍà¾× èÍ¹Óä»¾Ñ²¹Òà·¤¹Ô¤กÒÃÇÔ¹Ô¨©ÑÂâÃ¤¹ÕâÍÊ»ÍâÃ«ÔÊã¹â¤กçä´é ËÃ×Í¨Ð¹Óàª× éÍä»·ÓกÒÃ·´ÅÍ§à¾× èÍÈÖกÉÒãËéà¢ éÒã¨¶Ö§ªÕÇÇ Ô·ÂÒËÃ×ÍกÅäกกÒÃกèÍâÃ¤¢Í§ÁÑ¹à¾Ô èÁ¢Ö é¹กçä´é ËÃ×ÍÍÒ¨¨Ð¹Óàª× éÍä»ãªéã¹กÒÃ·´ÅÍ§ËÒÂÒÃÑกÉÒâÃ¤µÑÇãËÁèËÃ×Í¼ÅÔµÇÑ¤«Õ¹ÊÓËÃÑº»éÍ§กÑ¹âÃ¤

à·èÒ·Õ è·ÃÒºã¹»Ñ¨¨ØºÑ¹àª× éÍ N.caninum ÁÕ 3 ÃÐÂÐã¹Ç§ªÕÇ Ôµ¤×Í tachyzoite, bradyzoite

áÅÐ oocyst « Ö è§ÊÓËÃÑº¤ÇÒÁà»ç¹ä»ä´éã¹กÒÃà¾ÒÐàÅÕ éÂ§àª× éÍã¹ËéÍ§·´ÅÍ§à¾× èÍ·Õ è¨Ðä´é¹Óä»ãªé»ÃÐâÂª¹ìã¹กÒÃÈÖกÉÒã¹ÃÐ´Ñº·Õ èÊ Ù§¢ Ö é¹àªè¹ ÈÖกÉÒàกÕ èÂÇกÑºกÅäกกÒÃกèÍâÃ¤ËÃ×ÍÈÖกÉÒàกÕ èÂÇกÑº¤ÇÒÁäÇµèÍÂÒ¹Ñ é¹ ÃÐÂÐ·Õ èÊÒÁÒÃ¶¶ÙกàÅÕ éÂ§´ÙáÅÐà¾ÒÐàÅÕ éÂ§ä´é§ èÒÂ·Õ èÊ Ø´ã¹¢³Ð¹Õ éÁ Õà¾ÕÂ§ÃÐÂÐtachyzoite à·èÒ¹Ñ é¹ à¹× èÍ§¨ÒกÁÑ¹à»ç¹ÃÐÂÐ·Õ èÁ ÕกÒÃáºè§µÑÇà¾Ô èÁ¨Ó¹Ç¹ä´éÃÇ´àÃçÇ·Õ èÊ Ø´ ÊèÇ¹ÃÐÂÐbradyzoite ¹Ñ é¹à»ç¹ÃÐÂÐ·Õ èÁÕกÒÃáºè§µÑÇÍÂèÒ§ªéÒæ áÅÐ¨ÐàกÔ´¢Ö é¹ÀÒÂãµéÊÀÒÇÐกÒÃà»ÅÕ èÂ¹á»Å§ËÃ×Íก´´Ñ¹ºÒ§ÍÂèÒ§ ´Ñ§¹Ñ é¹กÒÃà¾ÒÐàÅÕ éÂ§ã¹à«ÅÅì¨Ö§·Óä´éÂÒกกÇèÒ ÁÕÃÒÂ§Ò¹ÇèÒã¹กÒÃà¾ÒÐàÅÕ éÂ§àª× éÍÃÐÂÐ tachyzoite ¢Í§ T.gondii «Ö è§à»ç¹àª× éÍ·Õ èÁÕÃ Ù»ÃèÒ§áÅÐªÕÇÇÔ·ÂÒ¤ÅéÒÂกÑ¹กÑº N.caninum

ÀÒÂã¹à«ÅÅìà¾ÒÐàÅÕ éÂ§¹Ñ é¹ ¨ÐÁÕºÒ§à«ÅÅì·Õ èà»ÅÕ èÂ¹ä»กÅÒÂà»ç¹ÃÐÂÐ bradyzoite »¹ÍÂÙ èก ÑºÃÐÂÐtachyzoite ÀÒÂã¹à«ÅÅìà¾ÒÐàÅÕ éÂ§ä´é´ éÇÂáµèก çà»ç¹ÊÑ´ÊèÇ¹¨Ó¹Ç¹·Õ è¹ éÍÂÁÒก (Dubey et al.,1998) áÅÐà¹×èÍ§¨Òก bradyzoite ÁÕÃÙ»ÃèÒ§·Õ è¤ÅéÒÂกÑ¹ÁÒกกÑºÃÐÂÐ tachyzoite กÒÃ¨ÐáÂกáÂÐÇèÒà«ÅÅìã´à»ç¹ÃÐÂÐ tachyzoite ËÃ×Íà«ÅÅìã´à»ç¹ÃÐÂÐ bradyzoite ·Óä´éäÁè§ èÒÂâ´ÂÍÒ¨ãªéกÅéÍ§phase contrast ËÃ×ÍÂéÍÁà«ÅÅì´éÇÂÊÕ Giemsa, ÊÕ PAS, ÊÕ silver stains à¾×èÍ´ÙกÒÃµÔ´ÊÕ·Õè¼¹Ñ§à«ÅÅìËÃ×ÍãªéáÍ¹µÔºÍ´Õ·Õ èà©¾ÒÐµèÍÃÐÂÐÁÒÂéÍÁµÃÇ¨¨Ñº¼ÔÇà«ÅÅì (immunostaining) ËÃ×ÍกÒÃãªéกÅéÍ§ØÅ·ÃÃÈ¹ìÍÔàÅกµÃÍ¹ TEM (Dubey et al., 1998)

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ÍÕกÇÔ¸ÕË¹Ö è§·Õ èÍÒ¨¨Ðãªéä´éÊÓËÃÑºกÒÃ·Õ è¨Ð¼ÅÔµÃÐÂÐ bradyzoite ¢Í§ N.caninum ¢Ö é¹ÁÒกç¤×ÍกÒÃ©Õ´àª× éÍà¢éÒä»ã¹ÊÑµÇì·´ÅÍ§àªè¹ Ë¹Ù mice, Ë¹Ù gerbil àª× éÍ¨Ð¶ÙกÀÙÁÔ¤Ø éÁกÑ¹¢Í§Ë¹Ùก´´Ñ¹¨¹à»ÅÕ èÂ¹ä»กÅÒÂà»ç¹ÃÐÂÐ bradyzoite á½§µÑÇÍÂÙ èÀÒÂã¹ÍÇÑÂÇÐµèÒ§æ áµèÍÂèÒ§äÃกçµÒÁÇÔ¸ Õ¹ Õ é¤ èÍ¹¢éÒ§ãªéàÇÅÒ¹Ò¹áÅÐä´é¼ÅäÁèá¹è¹Í¹à¹× èÍ§¨ÒกË¹Ù·´ÅÍ§ÁÑกäÁè¤èÍÂÁÕ¤ÇÒÁäÇµèÍกÒÃกèÍâÃ¤¢Í§ N.caninum (Gottstein et al., 2000)

ÊèÇ¹กÒÃà¾ÒÐàÅÕ éÂ§ËÃ×Í¼ÅÔµ oocyst ¹Ñ é¹ÂÔ è§à»ç¹ä»ä´éÂÒกÁÒกà¹× èÍ§¨ÒกâÎÊµìÊ Ø´·éÒÂà·èÒ·Õ è·ÃÒºกÑ¹·ØกÇÑ¹¹Õ éÁ Õà¾ÕÂ§á¤èÊ Ø¹ Ñ¢ºéÒ¹áÅÐ coyote à·èÒ¹Ñ é¹ áÅÐกÒÃ¼ÅÔµ oocyst ¹Ñ é¹¨ÐàกÔ´¢Ö é¹ÀÒÂã¹ÅÓäÊé¢Í§ÊØ¹Ñ¢áµèกçÂ Ñ§äÁè·ÃÒºÃÒÂÅÐàÍÕÂ´ÇèÒÁÕกÃÐºÇ¹กÒÃËÃ×ÍÅÓ´Ñº¢Ñ é¹µÍ¹à»ç¹ÍÂèÒ§äÃºéÒ§ ¨ÒกกÒÃ·´ÅÍ§·ÓãËéÊ Ø¹ Ñ¢µÔ´àª× éÍ¹ÕâÍÊ»ÍÃèÒáÅéÇ¹ÓÍØ¨¨ÒÃÐ¢Í§ÊØ¹Ñ¢ÁÒµÃÇ¨ËÒoocyst ¹Ñ é¹¾ºÇèÒÊØ¹Ñ¢ÊÒÁÒÃ¶¼ÅÔµ oocyst ä´éà»ç¹¨Ó¹Ç¹äÁèÁÒก¹ÑกáÅÐªèÇ§ÃÐÂÐàÇÅÒ·Õ è¨Ð¢Ñºoocyst ÍÍกÁÒกç¤èÍ¹¢éÒ§ÊÑ é¹áÅÐäÁèá¹è¹Í¹ÍÕก´éÇÂ (McAllister et al., 1998; Lindsay et al.,1999; Dubey, 2003; Dubey et al., 2007)

àª× éÍ N.caninum ÃÐÂÐ tachyzoite ·Õ èä´é¨ÒกกÒÃà¾ÒÐàÅÕ éÂ§¤ÃÑ é§¹Õ éä´é¶Ùก¹Óä»ãªé·´ÅÍ§ËÒÂÒÃÑกÉÒâÃ¤µÑÇãËÁè (Wiengcharoen et al., 2007) áÅÐ¹Óä»ãªéÊÓËÃÑº·´ÅÍ§·ÓãËéàกÔ´âÃ¤ã¹â¤¹ÁµÑé§·éÍ§áÅéÇ éÇÂ

¤³Ð¼Ù éà¢ ÕÂ¹¢Í¢Íº¤Ø³â¤Ã§กÒÃ»ÃÔÒàÍกกÒ¨¹ÒÀÔàÉกÊÓËÃÑº¤èÒãªé¨ èÒÂ·Õ èãª éã¹กÒÃ·´ÅÍ§¤ÃÑé§¹Õé

Anderson M.L., Andrianarivo A.G., Conrad P.A. 2000. Neosporosis in cattle. Anim

Reprod Sci.60-61:417-31.Bergeron N., Fecteau G., Villeneuve A., Girard C., Pare J. 2001. Failure of dogs to

shed oocysts after being fed bovine fetuses naturally infected by Neospora

caninum. Vet Parasitol. 97:145-52.

Bjerkas I., Mohn S.F., Presthus J. 1984. Unidentified cyst-forming sporozoon causing

encephalomyelitis and myositis in dogs. Z. Parasitenkd. 70:271-4.Buxton D., McAllister M.M., Dubey J.P. 2002 . The comparative pathogenesis of

neosporosis. Trends Parasitol.18:546-52.Dubey J.P. 2003. Review of Neospora caninum and neosporosis in animals. Korean. J

Parasitol. 41:1-16.

Dubey J.P., Carpenter J.L., Speer C.A., Topper M.J., Uggla A. 1988. Newly recognized

fatal protozoan disease of dogs. J Am Vet Med Assoc. 192:1269-85.

กÔµµÔกÃÃÁ»ÃÐกÒÈ

àÍกÊÒÃÍéÒ§ÍÔ§

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Dubey J.P., Lindsay D.S., Speer C.A. 1998. Structures of Toxoplasma gondii

tachyzoites, bradyzoites, and sporozoites and biology and development of tissue

cysts. Clin Microbiol Rev. 11:267-99.Dubey J.P., Buxton D., Wouda W., 2006. Pathogenesis of bovine neosporosis. J

Comp Path. 134:267-89.Dubey J.P., Schares G., Ortega-Mora L.-M., 2007. Epidemiology and control of

neosporosis and Neospora caninum. Clin Microbiol Rev. 20:323-67.Gottstein H.B., Conraths F.J., De Meerschman F., Ellis J.T., Innes E.A., McAllister M.M,

Ortega-Mora L.-M., Tenter A.M., Trees A.J., Uggla A., Williams D.J.L., Wouda W.

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Southeast Asian J Trop Med Public Health. 38 (suppl 1):15-18.



20

Cultivation of Neospora caninum in vero cell

Jitbanjong Wiengcharoen1,#, Ryan O’Handley2, Andrew Thompson2,

Parntep Rattanakorn3 and Yaowalark Sukthana4

Abstract

Neospora caninum was first reported as the causative agent of

encephalomyelitis in dog since 1984. Nowaday, it was recognized as a protozoa which

can cause an important reproductive disease, neosporosis, in cattle throughout the

world. It can also cause disease in dogs, goats, sheep, horses and deer. There were

many reports concerned with neosporosis in cattle but its life cycle, biology and

pathogenesis was not yet completely understood until now. The cultivation of

N.caninum in cell culture would be very useful because the parasite can be used as the

source of antigen and DNA for the diagnostic purpose. Moreover, the lived parasite can

be used to infect the animals in the experiment to get more understanding about its

biology and pathogenesis. This study is the first report in Thailand that success to

culture the tachyzoite stage of N.caninum strain K9WA in vero cell.

Keywords : Neospora caninum, neosporosis, tachyzoite, vero cell

1Faculty of Veterinary Medicine, Mahanakorn University of Technology2Division of Veterinary and Biomedical Sciences, Murdoch University, Western Australia3Faculty of Veterinary Sciences, Mahidol University4Department of Protozoology, Faculty of Tropical Medicine, Mahidol University#Corresponding Author / E-mail: [email protected].

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et al., 2006) äÁèà¾ÕÂ§áµèàª× éÍâÃ¤»ÒกáÅÐà·éÒà»× èÍÂ¨ÐÁÕกÒÃá¾ÃèกÃÐ¨ÒÂä»กÑº½Ù§ÊÑµÇìà·èÒ¹Ñ é¹ÂÑ§ÁÕËÅÑก°Ò¹·ÕèáÊ´§ ãËéàËç¹ÇèÒกÒÃà¤Å×èÍ¹ÂéÒÂÊÑµÇì·ÓãËéàกÔ´กÒÃกÒÃá¾ÃèกÃÐ¨ÒÂ¢Í§àª×éÍäÇÃÑÊ¼èÒ¹·Ò§ÊÔè§Í× è¹æ ä´éáกèกÒÃ »¹à»× éÍ¹กÑºÂÒ¹¾ÒË¹ÐáÅÐºØ¤¤Åà¤Å× èÍ¹ÂéÒÂÁÒ¾ÃéÍÁ½Ù§ÊÑµÇì (Gibbens et al.,2001)

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et al. (1996) ¾ºÇèÒกÃÐº×ÍÊÒÁÒÃ¶¤§ÊÀÒ¾à»ç¹¾ÒËÐ¢Í§âÃ¤»ÒกáÅÐà·éÒà»× èÍÂä´éà»ç¹àÇÅÒ¹Ò¹áÅÐ¾ºÍÑµÃÒกÒÃà»ÅÕ èÂ¹á»Å§·Ò§¾Ñ¹¸ØกÃÃÁ¢Í§àª× éÍ FMDV ã¹กÃÐº×Í¤èÍ¹¢éÒ§ÊÙ§ «Ö è§ÅÑกÉ³Ð¾Ñ¹¸ØกÃÃÁ¢Í§àª× éÍäÇÃÑÊ·Õ èà»ÅÕ èÂ¹á»Å§ä»¹Õ é¨ÐÁÕ¼ÅµèÍ»ÃÐÊÔ·¸Ô¼Å¢Í§กÒÃãªéÇÑ¤«Õ¹à¾× èÍ»éÍ§กÑ¹âÃ¤»ÒกáÅÐà·éÒà»×èÍÂã¹½Ù§ÊÑµÇìÍÕก éÇÂ

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µÃÇ¨ËÒ FMDV ·Øก«ÕâÃä·»ì ´Ñ§¹Ñ é¹¨Ö§¤ÇÃÁÕกÒÃµÃÇ¨áÂก«ÕâÃä·»ì¢Í§àª× éÍ FMDV ·Õ èµÃÇ¨¾ºã¹¾×é¹·Õ è´éÇÂÇÔ¸Õ Liquid phase blocking ELISA ä»¾ÃéÍÁกÑ¹´éÇÂà¾× èÍãªéã¹กÒÃ¾Ô¨ÒÃ³ÒàÅ×ÍกกÒÃãªéÇÑ¤«Õ¹ãËéáกèÊÑµÇìã¹¾× é¹·Õ èãËéµÃ§กÑº«ÕâÃä·»ì·Õ èµÃÇ¨¾º à¾× èÍà»ç¹กÒÃà¾Ô èÁ»ÃÐÊÔ·¸ÔÀÒ¾¢Í§ÁÒµÃกÒÃกÒÃ¤Çº¤ØÁáÅÐ»éÍ§กÑ¹âÃ¤»ÒกáÅÐà·éÒà»×èÍÂã¹¾×é¹·ÕèãËéÁÒกÂÔè§¢Öé¹

¢Í¢Íº¤Ø³à¨éÒË¹éÒ·Õ èÈÙ¹ÂìÇÔ¨ÑÂáÅÐ¾Ñ²¹ÒกÒÃÊÑµÇá¾·ÂìÀÒ¤µÐÇÑ¹µก กÃÁ»ÈØÊÑµÇì ã¹กÒÃµÃÇ¨ËÒàª×éÍ FMDV ÃÇÁ¶Ö§ ¹.Ê¾.´Ã.Á§¤Å ªÇ³Ôª áÅÐ ¹.Ê¾. ÁÃกµ Ç§ÈìË¹èÍ ÀÒ¤ÇÔªÒ¤ÅÔ¹Ôก ¤³ÐÊÑµÇá¾·ÂÈÒÊµÃì ÁËÒÇÔÂÒÅÑÂà·¤â¹âÅÂÕÁËÒ¹¤Ã ÊÓËÃÑº¤Óá¹Ð¹ÓµèÒ§æ ã¹กÒÃÈÖกÉÒ¤ÃÑé§¹Õé

¹ÔÃÑ¹´Ã àÍ×éÍ§µÃÐกÙÅÊØ¢ áÅÐ¨ÕÃÐ ÊÃ¹ØÇÑµÃ. 2541. áÍ¹µÔºÍ´ÕéµèÍâÃ¤»Òกà·éÒà»×èÍÂ¢Í§â¤-กÃÐº×Í·Õèà¤Å× èÍ¹ÂéÒÂ¼èÒ¹¨Ø´µÃÇ¨ÊÑµÇì ÍÓàÀÍËéÒ§©ÑµÃ ¨Ñ§ËÇÑ´ÅÓ»Ò§. ÃÒÂ§Ò¹กÒÃà½éÒÃÐÇÑ§·Ò§ÃÐºÒ´ÇÔ·ÂÒ. กÃÁ»ÈØÊÑµÇì. Ë¹éÒ 1-5.

ÇÔäÅ ÅÔ¹¨§ÊØº§กª ¹ØÈÃÒ ä¾·ÙÃÂìÇ§ÈìÇÕÃÐ áÅÐáÍº ¤§·¹. 2530. »ÃÐÁÇÅàÃ×èÍ§กÒÃ»ÃÐªØÁ·Ò§ÇÔªÒกÒÃ»ÈØÊ ÑµÇ ì ¤ÃÑ é§·Õ è 6. กÃÁ»ÈØÊ ÑµÇ ì. âÃ§¾ÔÁ¾ìª ØÁ¹ØÁÊËกÃ³ìกÒÃàกÉµÃáËè§»ÃÐà·Èä·Â ¨ÓกÑ .Ë¹éÒ 189-198.

ÇÔäÅ ÅÔ¹¨§ÊØº§กª áÅÐ¸¹ÃÑµ¹ì ¨Ò¹ØกÔ¨. 2537. กÒÃµÃÇ¨ËÒÃÐ´ÑºáÍ¹µÔºÍ´ÕéµèÍäÇÃÑÊâÃ¤»Òกà·éÒà»× èÍÂâ´ÂÇÔ¸ÕÊÔ¤ÇÔ´à¿Ê ÍÕäÅ«Ò. »ÃÐÁÇÅàÃ× èÍ§»ÃÐªØÁÇÔªÒกÒÃกÍ§¼ÅÔµÇÑ¤«Õ¹ªÕÇÀÑ³±ì ¤ÃÑ é§·Õè 1. กÃÁ»ÈØÊÑµÇì. âÃ§¾ÔÁ¾ìªØÁ¹ØÁÊËกÃ³ìกÒÃàกÉµÃáËè§»ÃÐà·Èä·Â ¨ÓกÑ´. Ë¹éÒ 63-72.

Í¹ÔÃØ¸ à¹×èÍ§àÁçก áÅÐÍÑ¨¨ÔÁÒ ¤ÅéÒÂË§Êì. 2549. áÍ¹µÔºÍ´ÕéµèÍâÃ¤»ÒกáÅÐà·éÒà»×èÍÂ¢Í§â¤�กÃÐº×Íã¹µÅÒ´¹Ñ´¤éÒÊÑµÇìã¹¾×é¹·ÕèÀÒ¤àË¹×ÍÃÐËÇèÒ§ ¾.È. 2542�2546. [Online]. Available: http://www.dld.go.th/pvlo_pyu/estate.doc

ÍÓ¾Ñ¹ Â§¾ÔÈÒÅÀ¾ áÅÐÇÃÒกÃ ¨ÔµÃËÅÑ§. 2549. กÒÃà½éÒÃÐÇÑ§âÃ¤»Òกà·éÒà»× èÍÂã¹â¤¹Óà¢éÒ·Õ è¨ Ñ§ËÇÑ´áÁèÎ èÍ§ÊÍ¹. ã¹ÃÇÁ¼Å§Ò¹ÇÔªÒกÒÃ·Õ èÊÓ¤Ñ»ÃÐ¨Ó»Õ àÅ èÁ·Õ è1. ÊÓ¹Ñก¤Çº¤ØÁ»éÍ§กÑ¹áÅÐºÓºÑ´âÃ¤ÊÑµÇì. กÃÁ»ÈØÊÑµÇì. âÃ§¾ÔÁ¾ìªØÁ¹ØÁÊËกÃ³ìกÒÃàกÉµÃáËè§»ÃÐà·Èä·Â ¨ÓกÑ . Ë¹éÒ 27-37.

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»·è 4 ©ºÑº·è 1 Á¡ÃÒ¤Á - ÁÔ¶Ø¹ÒÂ¹ 2552

Seroprevalence of Foot-and-Mouth disease in Cattle and

Buffaloes at the Kanchanaburi Animal Quarantine Station

during 2006-2007

Sorayut Seekhaow1, Apiradee Intarapuk2, #

Abstract

Foot and mouth disease (FMD) is a high contagious viral disease which endemic in

Thailand. This study focused on the Kanchanaburi Animal Quarantine Station, located on

Thai-Myanmar border, in which a large number of animals had immigrated from Myanmar.

Serum of cattle and buffaloes were collected from apparently healthy each month from

2006 to 2007. Samples were detected antibodies against FMDV by the Non-structural 3

ABC, 3B protein (NSP) ELISA assay that has greatly advanced sero-diagnosis/surveillance

as the test detects exposure to live virus for any the seven serotypes of FMDV, even in

vaccinated populations. Thoughtfully the study, the FMDV infectious prevalence was

10.29%, as well as 14.49% were found in 2006 and 3.67% were found in 2007. The

prevalence in 2006 was significantly higher than 2007 (p < 0.05). Cattle and

buffaloes were found infections in 8.6% and 12.4%, respectively. The statistical analysis

showed significantly difference of infectious rates between cattle and buffalo (p < 0.05).

Keywords : Foot and mouth disease, prevalence, non-structural protein, ELISA, Kanchanaburi

1Kanchanaburi International Animal Quarantine Station, Department of Livestock Development2Faculty of Veterinary Medicine, Mahanakorn University of Technology

#Corresponding author, E-mail : [email protected]



32

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¹Íก¨Òก¹Õ éÍÒ¨¨ÐËÒá¹Ç·Ò§ã¹กÒÃ¹Óä»¼ÅÔµà»ç¹¼ÅÔµÀÑ³±ìÍ × è¹æ·Õ èà» ç¹»ÃÐâÂª¹ì àª è¹กÒÃÊกÑ´áÂกà»ç¹¹éÓÁÑ¹»ÅÒ (fish oil) ÍÍกÁÒãªé â´ÂÊ¶ÒºÑ¹ÍÒËÒÃ (2006) ä´é·´ÊÍºä¢ÁÑ¹¾ºÇèÒ»ÅÒà¼ÒÐÁÕกÃ´ä¢ÁÑ¹·Õ è¨Óà»ç¹µèÍÃèÒ§กÒÂÁÒกกÇèÒ 10% Docosahexaenoic acid (DHA)2% áÅÐ Total Omega 3 ÁÒกกÇèÒ 3% «Öè§ÊÒÁÒÃ¶¹Óä¢ÁÑ¹·ÕèÊกÑ´ä´é¨ÒกàÈÉªÔé¹ÊèÇ¹»ÅÒà¼ÒÐ·ÕèàËÅ×Í¨ÒกกÒÃªÓáËÅÐÁÒÊกÑ´à»ç¹¼ÅÔµÀÑ³±ìÍÒËÒÃàÊÃÔÁà¾× èÍÊØ¢ÀÒ¾ÊÓËÃÑºÁ¹ØÉÂì«Ö è§à»ç¹กÒÃà¾Ô èÁÁÙÅ¤èÒãËéกÑºàÈÉªÔé¹ÊèÇ¹»ÅÒà¼ÒÐ·ÕèàËÅ×Í¨ÒกกÒÃªÓáËÅÐä éÍÕกÁÒก

ÍÂèÒ§äÃกçµÒÁกÒÃÈÖกÉÒ¤ÃÑ é§¹Õ é à» ç¹กÒÃÈÖกÉÒàº× éÍ§µé¹ ã¹กÒÃÈÖกÉÒÍÒ¨ãªéµ ÑÇÍÂèÒ§·Õ èÁ Õ»ÃÔÁÒ³äÁèÁÒก áµèกç·ÓãËéÊÒÁÒÃ¶»ÃÐÁÒ³กÒÃ¶Ö§¤Ø³¤èÒ·Ò§âÀª¹Ð¢Í§àÈÉªÔ é¹ÊèÇ¹»ÅÒà¼ÒÐ·Õ èàËÅ×Í¨ÒกกÒÃªÓáËÅÐä´é «Ö è§à»ç¹¾× é¹°Ò¹ÊÓ¤Ñã¹กÒÃÈÖกÉÒµèÍä»ã¹Í¹Ò¤µà¾× èÍกÒÃãªé»ÃÐâÂª¹ìÊÙ§ÊØ ¨Òก»ÅÒª¹Ô ¹Õé

¤³Ð¼Ù éÇ Ô¨ ÑÂ¢Í¢Íº¤Ø³ ¹.Ê.Ã Ø è§ÃÇ Õ áÊ§à»èÒ áÅÐ¹ÒÂÊØ¢ ØÁ Ç èÍ§¸¹ÒกØÅ ·Õ èãË é¤ÇÒÁªèÇÂàËÅ×Íã¹กÒÃÇÔà¤ÃÒÐËì¤Ø³¤èÒ·Ò§âÀª¹Ðã¹ËéÍ§»¯ÔºÑµÔกÒÃ áÅÐºÃÔÉÑ· ÂÙà¹ÕÂ¹ ÍกÃÕ¿ÒÃì ¨ÓกÑ´·ÕèãËé¤ÇÒÁªèÇÂàËÅ×Íã¹กÒÃàกçºµÑÇÍÂèÒ§ã¹กÒÃ·´ÅÍ§¤ÃÑé§¹Õé




38

¾Ã¾ÔÉ³Ø ¸ÃÃÁ»Ñ·Áì áÅÐ ¾ÑªÃÔ¹·Ãì ÃÐÇÕÂ Ñ¹. 2550. »ÅÒà¼ÒÐ : áËÅè§¢Í§âÍàÁกéÒ 3 áÅÐ»ÃÐâÂª¹ìµèÍÊØ¢ÀÒ¾. ã¹ : àÍกÊÒÃ»ÃÐกÍºกÒÃ»ÃÐªØÁÇÑ¹ÇÔªÒกÒÃÁËÒÇÔ·ÂÒÅÑÂàªÕÂ§ãËÁè¤ÃÑé§·Õè 3 ÇÑ¹·Õè 23 -25 ¾ÄÈ ÔกÒÂ¹ 2550. Ë¹éÒ 293.

ÇÔÇÑ²¹ì »ÃÒÃÁÀì áÅÐ ªÑÂÈÔÃÔ ÈÔÃÔกØÅ. 2538. กÒÃÈÖกÉÒªÕÇÇÔ·ÂÒºÒ§»ÃÐกÒÃ¢Í§»ÅÒâÁ§. àÍกÊÒÃÇÔªÒกÒÃ©ºÑº·Õè 22/2538. กÍ§»ÃÐÁ§¹éÓ × . กÃÁ»ÃÐÁ§. กÃÐ·ÃÇ§àกÉµÃáÅÐÊËกÃ³ì. 53 Ë¹éÒ

Ê¶ÒºÑ¹ÍÒËÒÃ. 2006. â¤Ã§กÒÃ¾Ñ²¹ÒÊÑµÇì¹ éÓàÈÃÉ°กÔ¨. [Online] Available :www.nfi.or.th/nfi/fish/plan.htm. 8/04/2009

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Economic Co – operation and Development. 36 p.


39


»·è 4 ©ºÑº·è 1 Á¡ÃÒ¤Á - ÁÔ¶Ø¹ÒÂ¹ 2552

Preliminary Study: Nutritional Value of By – Product

from Pangasius bocourti Processing

Chanathip Thammakarn1,# , Nahatai Vijitrotai1 and Chunya Kongrith1

Abstract

Nutritional value of by – product from Pangasius bocourti processing was studied.

The by – product compose of fish meat together with fish head and spine after filet

separated out. Seven samples were randomly collected and three fishes in each. One of

these samples was collected as fresh sample and the six other samples were kept as

partially dried. Proximate analysis method was used for determining the nutritional value

moisture, dry matter, ash, ether extract, crude protein. Crude fiber, calcium and

phosphorus were measured in partially dried samples only. Gross energy was determined

by Automatic Bomb Calorimeter. The results reveal that the fresh sample has 49.35%moisture, 50.65% dry matter, 4.13% ash, 31.88% ether extract and 13.41% crude protein.

The gross energy of the fresh sample is 3,687 kcal/kg. For the partially dried samples, the

averages of nutritional value are 1.47% moisture, 98.53% dry matter, 7.58% ash,

46.38% ether extract, 46.93% crude protein, 0.34 of crude fiber, 6.12% calcium, 0.62%phosphorus and gross energy density equals 6,770 kcal/kg. The nutritional value

disclosed that the by – product from Pangasius bocourti processing is possible to apply

for animal feed. As the results of its nutritional value, the by - product can be used for

energy and protein source and may serve as a raw material ground head and fish spine,

because of high content of calcium and phosphorus. Therefore, it is possible to use the

partially dried by – product as a raw material or for developing products for human.

Keywords : Nutritional value, By – product, Pangasius bocourti

1Division of Animal Production Technology and Fisheries, Faculty of Agricultural Technology, King Mongkut’s Institute of

Technology Ladkrabang

#Corresponding author : E-mail : [email protected]



40

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2S ºÑ¹·Öก¼ÅÇèÒÁÕ

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2S ( Ñ§áÊ´§ã¹µÒÃÒ§·Õè 3)

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2S ( Ñ§áÊ´§ã¹µÒÃÒ§·Õè 3)

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46

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47


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¨Ø±ÒÃÑµ¹ì àÈÃÉ°กØÅ Ò³Ô¹ âÍÀÒÊ¾Ñ²¹กÔ¨ áÅÐ¤Áá¢ ¾ÔÅÒÊÁºÑµÔ. 2545. กÒÃÅ´¢Ñé¹µÍ¹กÃÐºÇ¹กÒÃ¨Ñ´กÒÃà¹×éÍÊÑµÇì¨ÒกกÒÃªÓáËÅÐ«ÒกÍØè¹ÃèÇÁกÑºกÒÃºèÁà¹×éÍà¾×èÍ»ÃÑº»ÃØ§¤Ø³ÀÒ¾à¹×éÍâ¤¢Ø¹. ÇÒÃÊÒÃÊÓ¹Ñก§Ò¹¤³ÐกÃÃÁกÒÃÇÔ ÑÂáËè§ªÒµÔ 34 (1) : 75-94.

·È¾Å ÍÁÃÈÔÃÔÇÑ²¹กØÅ. 2532. กÒÃ»ÃÐªØÁÊÑÁÁ¹ÒàÃ×èÍ§กÒÃÊè§àÊÃÔÁÊØ¢ÅÑกÉ³ÐกÒÃá»ÃÃÙ»ÊÑµÇì¹éÓàº×éÍ§µé¹¤ÃÑé§·Õè 1 ³ Ê¶ÒºÑ¹ÇÔ ÑÂÇÔ·ÂÒÈÒÊµÃìÊÒ¸ÒÃ³ÊØ¢. 14 ÁÔ¶Ø¹ÒÂ¹ 2532. ¹. 2.

¹§ÅÑกÉ³ì ÊØÇÃÃ³¾Ô¹Ô¨ áÅÐ»ÃÕªÒ ÊØÇÃÃ³¾Ô¹Ô¨. 2544. ¨ØÅªÕÇÇÔ·ÂÒ·ÑèÇä»3. ÊÓ¹Ñก¾ÔÁ¾ìáËè§¨ØÌÒÅ§กÃ³ìÁËÒÇÔ·ÂÒÅÑÂ, กÃØ§à·¾Ï. 589 ¹.

ÁÒÅÑÂ ºØÃÑµ¹กÃกÔ . 2547. กÒÃ»¹à»×éÍ¹ กÒÃ¤Çº¤ØÁáÅÐกÒÃµÃÇ¨ÇÔ¹Ô ©ÑÂ Salmonella, ¹. 1-20. กÒÃÇÔà¤ÃÒÐËìáº¤·ÕàÃÕÂ·Õè·ÓãËéàกÔ´âÃ¤ã¹ÍÒËÒÃ. 4-6 ÊÔ§ËÒ¤Á 2547. Ê¶ÒºÑ¹¤é¹¤ÇéÒáÅÐ¾Ñ²¹Ò¼ÅÔµÀÑ³±ìÍÒËÒÃ ÁËÒÇÔ·ÂÒÅÑÂàกÉµÃÈÒÊµÃì, กÃØ§à·¾Ï.

ÊÔÃÔ¾Ã Ê¸¹àÊÒÇÀÒ¤Âì. 2539. ØÅÔ¹·ÃÕÂìã¹¹ÁáÅÐ¼ÅÔµÀÑ³±ì¹Á, ¹. 16-25. ã¹ Ê¶ÒºÑ¹¤é¹¤ÇéÒáÅÐ¾Ñ²¹Ò¼ÅÔµÀÑ³±ìÍÒËÒÃ, กÒÃµÃÇ¨ÇÔà¤ÃÒÐËì ØÅÔ¹·ÃÕÂìã¹ÍÒËÒÃ. ÁËÒÇÔ·ÂÒÅÑÂàกÉµÃÈÒÊµÃì, กÃØ§à·¾Ï.

ÊØÁÒÅÕ ºØÁÒ ÍÃØ³ ºèÒ§µÃÐกÙÅ¹¹·ì ¹¾ÃÑµ¹ì ËÁÒ¹ÃÔÁ áÅÐªØÁ¾¨µì ÍÁÒµÂกØÅ. 2539. กÒÃµÃÇ¨ËÒàª×éÍ«ÑÅâÁà¹ÅÅèÒã¹¼ÅÔµÀÑ³±ì Òกà¹×éÍÊÑµÇìâ´ÂÇÔ Õ Standard Conventional áÅÐÇÔ Õ Modified Semisolid

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áËè§ªÒµÔ ÁกÍª. 6000-2547.Bangtrakulnonth, A., Boonmar, S., Marnrim, N., Leungyoslouechakul, S., Sutanthavibul, J. and Kusum,

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48

Meat Quality Examination of Pork and Beef in Local Fresh Market

and Supermarket in Muang District and Hun Hin District of

Phetchaburi Province.

Bhutharit Vittayaphattananurak Raksasiri1 and Rachakris Lertpatarakomol2

Abstract

Hygiene factor examination of pork and beef meat was studied in Local fresh Market and

Supermarket in Muang District and Hun Hin District of Phetchaburi Province. Total microbial count by

using total plate count and isolation of Sallmonella spp. was carried on in laboratory. The results from

total count of microbial contamination (CFU/g) in pork meat from local fresh market in Muang district

Phetchaburi Province was highly significance than supermarket meat in Muang district Phetchaburi

(5.92 x 105 CFU/g and 2.16 x 105 CFU/g, respectively) (P<0.01). Incontrast, supermarket in Hua

Hin district was highly significance than Hun Hin district (5.01 x 105 CFU/g and 1.68 x 105 CFU/g,

respectively) (P<0.01). Total microbial contamination (CFU/g) in Beef Meat from local fresh market in

Muang district Phetchaburi was highly significance than Supermarket in Muang district Phetchaburi

(5.44x105 CFU/g and 1.40x105 CFU/g, respectively) (P<0.01). Hua Hin district Market and supermarket

in Hua Hin district is not significantly difference. (4.47x105 CFU/g and 4.05 x 105 CFU/g, respectively)

(P<0.01). Isolation of Sallmonella spp. in pork and beef from local fresh market and supermarket in

Muang district Phetchaburi Province and Hun Hin district found that all tested samples was

contaminated by Sallmonella spp.

Keywords : Salmonella spp., pork, beef meat, microbial

1Faculty of Animal Science and Agricultural Technology, Silpakorn University Phetchaburi IT Campus.2Faculty of Veterinary Medicine, Mahanakorn University of Technology.#Corresponding author.

49


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What’s your cytological diagnosis?

ËÑÊ Ô¹·Ãì ºØÈÃÕâÃ¨¹ì1 áÅÐ ·¹§ÈÑก Ôì ÁÐÁÁ1, 2

1ÊÒ¢ÒÇÔªÒ¾ÂÒ¸ÔÇÔ·ÂÒ ¤³ÐÊÑµÇá¾·ÂÈÒÊµÃì ÁËÒÇÔ·ÂÒÅÑÂà·¤â¹âÅÂÕÁËÒ¹¤Ãà¢µË¹Í§¨Íก กÃØ§à·¾Ï 10530

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¨ÒกÃÙ» áÅÐ ¢éÍÁÙÅ»ÃÐÇÑµÔÊÑµÇì»èÇÂ §ãËéกÒÃÇÔ¹Ô ©ÑÂ·Ò§à«ÅÅìÇÔ·ÂÒ

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1âÃ§¾ÂÒºÒÅÊÑµÇì ¤³ÐÊÑµÇá¾·ÂÈÒÊµÃì ÁËÒÇÔ·ÂÒÅÑÂ¢Í¹áกè¹ ÍÓàÀÍ àÁ×Í§ Ñ§ËÇÑ ¢Í¹áกè¹ 40002#¼ÙéÃÑº¼Ô ªÍºº·¤ÇÒÁ: E-mail: [email protected]



52

âÃ¤ Trypanosomiasis àกÔ´¨Òกàª× éÍ·ÃÔ»¾Òâ¹â«Á«Ö è§à»ç¹ÊÑµÇìà«ÅÅìà´ÕÂÇ ÁÕÃ Ù»ÃèÒ§áº¹ÂÒÇËÃ×ÍÃÙ»ÃèÒ§กÅÁ ¹ÔÇà¤ÅÕÂÊ¢¹Ò´ãËèÍÂÙ èµÃ§กÅÒ§ áÅÐÁÕäÁâµÃ¤Í¹à´ÃÕÂ¢¹Ò´ãËè ¾ºä¤¹Õâµ¾ÅÒÊ (kinetoplast) «Ö è§»ÃÐกÍº´éÇÂ DNA ¨Ó¹Ç¹ÁÒก ÁÕáÊé (flagellum) Â× è¹ä»·Ò§´éÒ¹Ë¹éÒà¾× èÍªèÇÂã¹กÒÃà¤Å× èÍ¹·Õ è áÊé¨ÐÂÖ´µÔ´กÑºÅÓµÑÇ´éÇÂ undulating membrane â´Â¨ÐÍÂÙ èã¹กÃÐáÊâÅËÔµ ¹éÓä¢ÊÑ¹ËÅÑ§ áÅÐ¹éÓàËÅ×Í§¢Í§ÊÑµÇì·Õ èÁÕกÃÐ´ÙกÊÑ¹ËÅÑ§ áµèºÒ§ÃÐÂÐÍÒ¨à¢éÒä»ÍÂÙ èã¹à¹× éÍàÂ× èÍ â´ÂÁÕáÁÅ§´Ù´àÅ×Í´à»ç¹¾ÒËÐ¹ÓâÃ¤ ÁÑกÁÕกÒÃáºè§µÑÇËÃ×Íà»ÅÕ èÂ¹ÃÙ»ÃèÒ§ÍÂÙ èã¹¾ÒËÐ¹ÓâÃ¤ ºÒ§ª¹Ô กçäÁèÁÕกÒÃà»ÅÕèÂ¹ÃÙ»ÃèÒ§ ËÃ×Íà¾ÔèÁ¨Ó¹Ç¹ã¹¾ÒËÐ¹ÓâÃ¤áµèÍÂèÒ§ã´

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membrane) áÅÐàÂ×èÍµÒ¢ÒÇ (conjunctiva) ÁÕกÒÃÂ×è¹ÍÍกÁÒÁÒก¼Ô´»กµÔ àÁ×èÍâÃ¤ÅØกÅÒÁä»ÁÒก¢Öé¹ÊÑµÇì¨ÐàÃÔ èÁäÁèกÔ¹ÍÒËÒÃ ·èÒà´Ô¹¨Ð¼Ô´»กµÔä» ÍÑµÃÒกÒÃËÒÂã¨áÅÐªÕ¾¨Ã¨Ðà¾Ô èÁ¢Ö é¹ µèÍ¨Òก¹Ñ é¹»ÃÐÁÒ³ 3 ÇÑ¹ ÊÑµÇì¨ÐàÃÔ èÁÁÕÍÒกÒÃ·Ò§»ÃÐÊÒ· ¢Ò¨ÐàÃÔ èÁºÔ´àกÃç§ áÅÐ coma ºÒ§¤ÃÑé§ÊÑµÇìÍÒ¨ÁÕÍÒกÒÃ´ØÃ éÒÂ¤ÅéÒÂæกÑºã¹âÃ¤¾ÔÉÊØ¹Ñ¢ºéÒ กÒÃàกÔ´âÃ¤ã¹ÊØ¹Ñ¢ÍÒ¨àกÔ´ÁÒ¨ÒกกÒÃกÔ¹à¹× éÍ´Ôº·Õ èÁ Õàª×éÍª¹Ô ¹ÕéÍÂÙè (Brun et al., 1998; ÁÒ¹¾, 2540)

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ÃÑกÉÒ·Õ èâÃ§¾ÂÒºÒÅÊÑµÇì ¤³ÐÊÑµÇá¾·ÂÈÒÊµÃì ÁËÒÇÔ·ÂÒÅÑÂ¢Í¹áกè¹ àÁ× èÍÇÑ¹·Õ è 26 กÃก®Ò¤Á¾.È. 2550 ´éÇÂÍÒกÒÃºÇÁ¹éÓºÃÔàÇ³¤Ò§ ËÍº ÁÕä¢é àÂ× èÍàÁ×ÍกÁÕÊÕàËÅ×Í§«Õ´ à»ç¹ÁÒ»ÃÐÁÒ³ 1à´×Í¹ ÊØ¹Ñ¢à¤Âä´éÃÑºกÒÃÃÑกÉÒÁÒ¨Òก·Õ èÍ × è¹áÅéÇËÅÒÂ¤ÃÑ é§ «Ö è§ÍÒกÒÃกçÂÑ§äÁè´Õ¢Ö é¹ àÅÕ éÂ§ã¹à¢µâÃ§¦èÒÊÑµÇìáÅÐÁÕ»ÃÐÇÑµÔà¤ÂกÔ¹à¹×éÍ Ôº

กÒÃµÃÇ¨ÃèÒ§กÒÂÍØ³ËÀÙÁÔÃèÒ§กÒÂ 104 Í§ÈÒ¿ÒàÃ¹äÎµì ÁÕÍÒกÒÃ«ÖÁ ÁÕÊÕàÂ× èÍàÁ×Íกà»ç¹ÊÕàËÅ×Í§«Õ´ ËÒÂã¨

ËÍº ÁÕÍÑµÃÒกÒÃËÒÂã¨ 57 ¤ÃÑ é§µèÍ¹Ò·Õ ÍÑµÃÒกÒÃàµé¹¢Í§ËÑÇã¨ 82 ¤ÃÑ é§µèÍ¹Ò·Õ ºÇÁ¹éÓºÃÔàÇ³¤Ò§ ÁÕÀÒÇÐáËé§¹éÓÃéÍÂÅÐ 5 ¤ÅÓªèÍ§·éÍ§¾ºÇèÒ ÁÕกÒÃ¢ÂÒÂ¢¹Ò´¢Í§µÑº

กÒÃÇÔ¹Ô ©ÑÂáÂกâÃ¤âÃ¤¾ÂÒ Ôã¹àÅ×Í´ (Blood parasite)âÃ¤©ÕèË¹Ù (Leptospirosis)âÃ¤µÑºÍÑกàÊº (Hepatitis)âÃ¤äµÇÒÂ (Renal failure)

àª×éÍ·ÃÔ»¾Òâ¹â«Á·ÕèÍÂÙèã¹กÃÐáÊàÅ×Í´ÃÙ»·Õè 2



54

Items Results Results Reference Values*

Day 0 Day 19

Hematology

CBC

PCV (%) 15 39 37-55

Hb (g%) 4.15 12.4 12-18

RBC count

(x106/mm

3)

2.15 5.58 5.5-8.5

WBC count

(x103/mm

3)

7.1 9.16 6-17

Platelet estimation Adequate

RBC morphology Polychromasia, Anisocytosis

Differential WBC

Band neutrophil

(cells/µl) - - 0-300

Neutrophils(cell/µl) 89% (6,327) 49% (4,488) 2,750-12,850

Lymphocyte(cell/µl) 6% (426) 36% (3,297) 430-5,800

Monocyte(cell/µl) 3% (213) 4% (366) 50-1,400

Eosinophil(cell/µl) 2% (412) 10% (916) 100 5 1,250

Blood parasite examination

Routine exam. Trypanosome evansi not found

Clinical chemistry

Creatinine (mg/dl) 0.97 0.87 0.6-1.4

ALT (GPT) (U/L) 1,837 53 4-91

Blood sugar (mg%) 95 - 70-110

Leptospira Ab Negative

�� : Kahn ( 2005)

¼ÅกÒÃµÃÇ¨·Ò§ËéÍ§»®ÔºÑµÔกÒÃ

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¼ÅกÒÃµÃÇ¨àÅ×Í´·Ò§ËéÍ§»®ÔºÑµÔกÒÃµÒÃÒ§·Õè 1

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anemia) »ÃÔÁÒ³ÎÕâÁâกÅºÔ¹µèÓกÇèÒàก³±ì»กµÔ »ÃÔÁÒ³àÁç´àÅ×Í´¢ÒÇÃÇÁáÅÐ¨Ó¹Ç¹¹ÔÇâµÃ¿ÔÅÍÂÙ èã¹àก³±ì»กµÔ ¨Ó¹Ç¹ÅÔÁâ¿«ÑÂµìµèÓกÇèÒàก³±ì»กµÔàÅçก¹éÍÂ ¹Íก¨Òก¹Õ éÂ Ñ§¾ºÃÙ»ÃèÒ§àÁç´àÅ×Í´á´§à»ç¹áºº polychromasia anisocytosis Ê èÇ¹กÒÃµÃÇ¨¤èÒ·Ò§ªÕÇà¤ÁÕ¢Í§àÅ×Í´¤èÒàÍç¹ä«ÁìÍÐÅÒ¹Õ¹ÍÐÁÔâ¹·ÃÒ¹Êà¿ÍÃìàÃÊ (ALT) ÊÙ§กÇèÒ»กµÔÁÒก áµè¤èÒ¤ÃÕàÍ·Ô¹Õ¹ÍÂÙ èã¹àก³±ì»กµÔ ÃÐ´Ñº¹éÓµÒÅã¹àÅ×Í´ÍÂÙ èã¹àก³±ì»กµÔ áÅÐä´éµÃÇ¨¾º»ÃÊÔµã¹กÃÐáÊàÅ×Í´ª¹Ô´Trypanosoma evansi ÊèÇ¹กÒÃµÃÇ¨ËÒáÍ¹µÔºÍ ÕµèÍàª×éÍ Leptospira spp. ãËé¼ÅÅº

¨Òก»ÃÐÇÑµ ÔÊ ÑµÇ ì» èÇÂ ÍÒกÒÃ áÅÐกÒÃµÃÇ¨ÃèÒ§กÒÂàº× éÍ§µé¹¾ºÇèÒÊØ¹ Ñ¢ÁÕä¢ éÊ Ù§áÅÐàÂ× èÍàÁ×ÍกàËÅ×Í§«Õ´ ·ÓกÒÃÃÑกÉÒâ´ÂãËéÊÒÃ¹éÓª¹Ô´ D5-½ S áÅÐãËéÂÒÅ´ä¢é Dipyrone ã¹¢¹Ò´ 25ÁÔÅÅÔกÃÑÁµèÍกÔâÅกÃÑÁ à¢ éÒ·Ò§ËÅÍ´àÅ×Í´´Ó ¨Òก¹Ñ é¹ãËéÂÒ»¯Ôª ÕÇ¹Ð cefazolin ã¹¢¹Ò´ 25ÁÔÅÅÔกÃÑÁµèÍกÔâÅกÃÑÁ à¢éÒ·Ò§ËÅÍ´àÅ×Í´´ÓªéÒæ ¼ÅกÒÃµÃÇ¨àÅ×Í´¾ºÇèÒÁÕ»ÃÊÔµã¹กÃÐáÊàÅ×Í´ª¹Ô´ Trypanosoma evansi áÅÐÁÕàÁç´àÅ×Í´á´§·Õ èÍÂÙ èã¹¤èÒµèÓÁÒก ÃÇÁä»¶Ö§ÁÕÍÒกÒÃËÒÂã¨ËÍº ¨Ö§ä´é¾Ô¨ÒÃ³ÒãËéËÒÊØ¹Ñ¢ÁÒ·ÓกÒÃ¶èÒÂàÅ×Í´ã¹ÇÑ¹ÃØ è§¢Ö é¹áÅÐ¨Ð·ÓกÒÃÃÑกÉÒ»ÃÊÔµã¹กÃÐáÊàÅ×Í´ã¹ÇÑ¹¶Ñ´ä» áÅÐä´éãËéÂÒ Silymarin ก Ô¹ËÅÑ§ÍÒËÒÃ ¤ÃÑ é§ÅÐ 1 àÁç´ Ç Ñ¹ÅÐ 2 ¤ÃÑ é§ ÂÒ»¯ÔªÕÇ¹Ð Cephalexin กÔ¹ËÅÑ§ÍÒËÒÃ ¤ÃÑé§ÅÐ 1 àÁç´ ÇÑ¹ÅÐ 2 ¤ÃÑé§ áÅÐÂÒºÓÃØ§àÅ×Í´ Folic-C กÔ¹ËÅÑ§ÍÒËÒÃ ¤ÃÑé§ÅÐ 1 àÁç ÇÑ¹ÅÐ 2 ¤ÃÑé§ ¹Ò¹ 7 ÇÑ¹

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Harvey, 2004) µÃÇ¨¾º¤èÒàÍ¹ä«Áì ALT ÊÙ§กÇèÒ»กµÔÁÒก ã¹ÊØ¹Ñ¢àÍ¹ä«Áì¹Õ é¾ºä´éÁÒก·Õ èÊØ´ã¹µÑº áÅÐã¹กÅéÒÁà¹× éÍÃÍ§Å§ÁÒ ÍÒ¨ºè§ªÕ é¶ Ö§ÇèÒàกÔ´¤ÇÒÁ¼Ô´»กµÔ·Õ èµÑº «Ö è§ÍÒ¨àกÔ´¨Òก¾ÂÒ¸ÔÊÀÒ¾·Õ èµ Ñºâ´ÂµÃ§ ËÃ×ÍÁÕÊÒàËµØÍ × è¹æ·Õ è·ÓãËéµ Ñº·Ó§Ò¹¼Ô´»กµÔ àª è¹ »ÃÊÔµã¹àÅ×Í´ ËÃ×ÍÍÒ¨ÁÕกÅéÒÁà¹×éÍ¶Ùก·ÓÅÒÂ àกÔ´กÒÃ½èÍÅÕº (¹ØÊÃÒ, 2548) «Öè§ÍÒ¨à»ç¹ÊÒàËµØ·ÓãËé¤èÒ ALT ÊÙ§กÇèÒ»กµÔÁÒกáÅÐµÃÇ¨¾º»ÃÊÔµã¹àÅ×Í´ä´éáกè Trypanosoma evansi « Ö è§ÁÕ¤ÇÒÁÊÑÁ¾Ñ¹¸ìก Ñº»ÃÐÇÑµ ÔáÅÐÍÒกÒÃ·Ò§¤ÅÔ¹Ôก ÃÇÁ·Ñ é§¼ÅกÒÃµÃÇ¨ËÒáÍ¹µÔºÍ´ÕµèÍàª× éÍ Leptospira spp. ãËé¼ÅÅº áÅÐ¤èÒ¤ÃÔàÍ·Ô¹Ô¹ÂÑ§ÍÂÙ èã¹àก³±ì»กµÔ ·ÓãËéÊÒÁÒÃ¶ÇÔ¹Ô¨©ÑÂáÂกâÃ¤©Õ èË¹ÙáÅÐâÃ¤äµÍÍกä»ä´é ¨ Ö§Ç Ô¹Ô¨©ÑÂÇèÒÊØ¹Ñ¢à»ç¹ Trypanosomiasis «Öè§กÒÃµÔ àª×éÍ Ñ§กÅèÒÇÍÒ¨à»ç¹ÊÒàËµØ¢Í§กÒÃàกÔ ÀÒÇÐâÅËÔµ¨Ò§·ÕèÃØ¹áÃ§ä é

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³Ã§¤ì ก Ô¨¾Ò³ÔªÂì. 2544. ¤ÅÔ¹Ôก»¯ÔºÑµ Ô. Ë¹èÇÂÍÒÂØÃกÃÃÁÊÑµÇìàÅ çก ÀÒ¤ÇÔªÒÍÒÂØÃÈÒÊµÃì¤³ÐÊÑµÇá¾·ÂÈÒÊµÃì ÁËÒÇÔ·ÂÒÅÑÂ¢Í¹áกè¹. 145 Ë¹éÒ

¹ØÊÃÒ ÊØÇÃÃ³âªµÔ. 2548. ÀÒÇÐÊÁ´ØÅ¢Í§àËÅÇ ÍÔàÅ çกâµÃäÅ·ì áÅÐ¤ÇÒÁ¼Ô´»ÃกµÔ¢Í§ÊÁ´ØÅกÃ´-´èÒ§ กÒÃ·´ÊÍºกÒÃ·Ó§Ò¹¢Í§µÑº. ÀÒ¤ÇÔªÒ¾ÂÒ¸ÔÊÃÕÃÇÔ·ÂÒ ¤³ÐÊÑµÇá¾·ÂÈÒÊµÃìÁËÒÇÔ·ÂÒÅÑÂ¢Í¹áกè¹. 75 Ë¹éÒ.

ÁÒ¹¾ ÁèÇ§ãËè. 2540. Ç Ô·ÂÒÊÑµÇìà«ÅÅìà´ÕÂÇ·Ò§ÊÑµÇá¾·Âì. ¤³ÐÊÑµÇá¾·ÂÈÒÊµÃìØÌÒÅ§กÃ³ìÁËÒÇÔ·ÂÒÅÑÂ. âÃ§¾ÔÁ¾ìáËè§ ØÌÒÅ§กÃ³ìÁËÒÇÔ·ÂÒÅÑÂ. 250 Ë¹éÒ

ÊØ·¸ÔÈÑก´Ô ì ¹¾ÇÔÙÇ§Èì. 2545. àÍกÊÒÃ»ÃÐกÍºกÒÃÊÍ¹àÃ× èÍ§ âÅËÔµÇÔ·ÂÒ¤ÅÔ¹Ôก·Ò§ÊÑµÇá¾·Âì.Ë¹èÇÂ¼ÅÔµàÍกÊÒÃ¤³ÐÊÑµÇá¾·ÂÈÒÊµÃì ÁËÒÇÔ·ÂÒÅÑÂ¢Í¹áกè¹. 148 Ë¹éÒ.

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for Parasitology. 1835 p.

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Kenya. 20-23 November,1978. 155 p.




60

Treatment of Trypanosomiasis in Dog

Eakkasit Barameechaithanun1,#, Pimsuda Suwannasaeng1, Nittaya Boonbal1,

Sudarat Pattanee1 and Sompong Hoisang1

Abstract

A two-years-old male Rottweiler 37.6 kg. body weight was brought to Khonkaen University

Veterinary Teaching Hospital with the complaint of fever, edema and panting. He was

treated by a previous veterinarian for 1 month without significant improvement. The

physical examination showed 7% dehydration, mucous membranes are pale and

jaundice, edema at mandible area and palpably enlarged liver. Hematological and

Biochemistry were unremarkable except for anemia and increased

alanineaminotransferase (ALT). Trypanosoma evansi was detected on a blood smear.

Based on clinical and laboratory findings, the dog was put on dipyrone, diminazene

aceturate, silymarin, cephalexin, folic-C, fluid therapy and given a blood transfusion. His

clinical symptoms improved and Trypanosoma evansi was not detected in a blood

smear after treatment.

Keywords: Trypanosome, Anemia, Jaundice

1Khonkaen University Veterinary Teaching Hospital, Khonkaen University, Khon Kaen, 40002#Corresponding author: E-mail: [email protected]

61


»·è 4 ©ºÑº·è 1 Á¡ÃÒ¤Á - ÁÔ¶Ø¹ÒÂ¹ 2552


Kazumi Taniguchi1,# and Kazuyuki Taniguchi2

1Laboratory of Veterinary Anatomy, School of Veterinary Medicine, Kitasato University,

23-35-1 Higashi, Towada, Aomori 034-8628, Japan2Laboratory of Veterinary Anatomy, Faculty of Agriculture, Iwate University, 3-18-8 Ueda,

Morioka, Iwate 020-8550, Japan

Correspondence author: Kazumi Taniguchi, DVM, Ph.D.

Phone: 81-176-23-4371 ext. 432

Fax: 81-176-23-8703

Email: [email protected]

Purpose

In this article, we describe the recent progress of taste receptor research in

the scientific community. As of late, research on taste receptors has made remarkable

progress. In the present review, we focus on taste receptors, paying special attention to

receptors for sweetness, bitterness and umami.

The Five Elements of Taste

Up to now, five elements of taste have been reported: sweetness, bitterness,

sourness, saltiness and umami. They are independent of each other. In addition to these

five elements, other factors, such as the texture and temperature of food as well as fat,

contribute to the final “taste”, thus resulting in a very complicated combination of

mitigating factors.

Sweetness: The sweetness receptors sense sugars and carbohydrates. Once we

eat sugars and/or carbohydrates, our bodies util ize them as sources of energy.

Sweetness receptors must have developed to sense molecules carrying information

regarding sources of energy for our bodies. As a result, humans crave sweet food, since

energy is essential to maintaining life. Everyone, even newborn babies, loves sweet foods.

Bitterness: The bitterness receptors sense molecules present in foods that contain

alkaloids or that are burnt black. Bitterness can alert us that a food is potentially toxic, so

we can stop ingestion before being poisoned. Many individuals do like some slightly bitter

º·¤ÇÒÁ



62

foods, such as dark chocolates, coffee, or beer. Adults “learn”, as they grow up, that

certain bitter foods are not toxic. However, small children usually do not like bitter foods.

Sourness: The sourness receptors sense concentrations of hydrogen ions in food,

alerting us that the food may be rotten or unripe. For example, un-ripened fruits are sour,

while ripened fruits are sweet. The result is that humans, as in the case of bitter foods,

resist consuming sour foods. Furthermore, as in the case of bitter foods, we learn that

some sour foods, such as yogurt, can be safe.

Saltiness: The saltiness receptors sense concentrations of sodium ions.

Mammalian bodies require sodium ions to maintain ion balance, which is essential for

their lives. Excess sodium intake, however, can prove harmful.

Umami: The umami, or savory receptors sense amino acids. Meat and fish contain

a lot of umami. Some vegetables, such as tomatoes, have a great deal of umami as well.

Humans do not “taste” proteins, because the molecular sizes of proteins are too big to

bind to receptors; instead, humans “sense” amino acids. Beef, for example, obtained from

a freshly slaughtered cow, is not that “tasty”. However, if the beef is aged at a low

temperature, enabling proteins to break down and, as a result, producing more amino

acids, the taste of the beef improves greatly. This is because animals and humans can

“sense” larger amount of amino acids. Amino acids, and therefore proteins, are essential

as the building blocks of life, so humans have evolved to crave umami.

History of Umami Research

The word “umami”, meaning savory taste, comes from the Japanese language. A

Japanese researcher first extracted a substance containing umami, monosodium

glutamate (MSG), from seaweed. The researchers who declared for the first time that

umami must be one of the taste elements, were also Japanese. Since then, Japanese

researchers have conducted a large portion of the ongoing umami research. At the early

stages of umami research, western researchers doubted the existence of umami.

However, umami has now been established worldwide as one of the five taste elements.

Umami Receptors and Inosine Monophosphate

The umami receptors sense not only amino acids, but also inosine

monophosphates (IMP) in the cytoplasm. IMP is generated by the degradation of

adenosine tri-phosphates (ATP). ATP breaks down, becoming first adenosine di-

63


»·è 4 ©ºÑº·è 1 Á¡ÃÒ¤Á - ÁÔ¶Ø¹ÒÂ¹ 2552

phosphate (ADP), then guanosine monophosphate (GMP), and finally IMP. The higher the

amount of IMP in a food, the better it tastes.

Another interesting aspect of IMP is the fact that the combination of IMP and amino

acids clearly has synergistic effects on umami reception. Food containing only amino

acids or only IMP can be relatively tasty. Taste improves greatly, however, when food

contains both of these substances.

Nerve responses of the Chorda tympani, connects taste buds to the brain, were

investigated after an administration of either (1) only IMP; (2) a single type of amino acid,

such as glutamic acid, serine, alanine, glycine, asparagine or lysine; or (3) combinations

of IMP and a type of amino acid (Nelson et al., 2002) The results demonstrated that the

level of nerve responses was raised when IMP was administered at the same time as the

amino acid. Also investigated were the effects of IMP on other substances, such as

sodium chloride and sucrose. The results revealed that IMP had no significant effect on

the nerve responses.

Humans generally combine IMP and amino acids in food preparation. For example,

in traditional Japanese cooking, we combine kelp soup and dried bonito soup to make a

more savory soup. This is the most common and basic method of Japanese cooking.

Researchers have reported that kelp is rich in glutamic acids, while dried bonito is rich in

IMP (Brand et al., 1989 ; Filer et al., 1979; Yamaguchi et al., 1991) As a result,

this traditional combination in Japanese cooking is, scientifically, a very reasonable way to

improve taste.

People utilize this idea when we deal with meat as well. The concentration of IMP

is greater in aged meat than in fresh meat. As for the reasons why we prefer aged meat,

we could suggest at least three: (1) softer texture, (2) a higher concentration of amino

acids, and (3) a higher concentration of IMP. The softer texture is brought by the

degradation of cells and tissues caused by proteinases, enzymes which localize in

lysosomes in cells alive. After the cells die, the lysosomal enzymes are released into the

cytoplasm and break down the cells/tissues. The higher concentration of amino acids is

brought on by the degradation of proteins. The higher concentration of IMP is brought on

by the degradation of ATP. The speed of the ATP degradation after slaughtering differs

greatly, depending on animal species. The amount of IMP reaches a maximum eight to

ten hours after death in yellowtail tuna (fish); three days after slaughter in pigs, and seven

to ten days after slaughter in cows.



64

Taste and Evolution

More than 25 different types of bitterness receptors have been discovered, but only

three have been found for sweetness. This suggests that the ability to sense various

types of bitterness might have once been essential for survival. If an animal or a species

lacked the ability to sense a potentially toxic bitterness, it risked death or extinction. Only

those who succeeded in sensing “danger” could have survived.

As for saltiness, animals, including humans, are very sensitive to slight differences of

salt concentrations in food. This sensitivity may be related to the evolution of vertebrates.

Vertebrates evolved from ancient ocean fish, then went ashore to become animals that

depended on fresh water. Salt concentrations in water must have been a matter of life or

death for early vertebrates. This ability to detect water salinity may retain importance,

even in today’s mammals.

Taste Receptors

Seven-transmembrane proteins and ion channels: There are two types of

receptors, these for bitterness, sweetness and umami, composed of seven-

transmembrane proteins coupled with GTP binding-proteins, and those for saltiness and

sourness, composed of ion channels. An alpha subunit of G-protein coupled with bitter

receptors, alpha-gustducin, was discovered by McLaughlin, et al. (1992) as shown in figure 1.

Taste receptors for sweetness, bitterness and umami are quite similar to those for

smells. Taste receptors are quite similar to olfactory receptors in their structures. There

are two types of olfactory receptors: (1) those on the olfactory epithelium lining the nasal

cavity; and (2) those on the sensory epithelium of the vomeronasal organ. The former are

for common smells, and the latter for pheromones. Both types of receptors are seven-

transmembrane proteins coupled with G-proteins, as are taste receptors. In both cases,

information on taste or olfaction is transferred to the brain, processed there, and

recognized as taste or smell.

T1Rs and T2Rs: Researchers have categorized taste receptors into two families:

T1Rs (T1R family) and T2Rs (T2R family). The “T” means “Taste”, and the “R” means

“Receptors”. T1Rs are sweetness and umami receptors, while T2Rs are bitterness

receptors (Chandrashekar et al., 2000; Kitagawa et al., 2001; Max et al., 2001; Nelson et al.,

2001)

Max et al. (2001) and Margolskee, R.F. (2002) suggested that T1Rs may take the

form of homo- or hetero-dimers (Margolskee, 2002; Max et al., 2001) This means that the

molecule is composed of two of the same or different units: the same units for the

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homodimer and different units for heterodimer. Nelson et al. (2002) proved that a

heterodimer, composed of T1R2 and T1R3, functions as a sweetness receptor while a

heterodimer, composed of T1R1 and T1R3, functions as an umami receptor.

Various methods have been utilized to prove the roles of taste receptors, such as

heterologous expression and neurophysiological investigations. One method is to make

taste genes express in cells which normally do not express them, such as human

embryonic kidney cells, culture them, and examined them for changes of calcium ion

concentrations after the administration of various types of taste substances. Another

method is to measure the level of nerve responses using electro-physiological techniques

after the administration of various taste substances on the tongue.

Hetero-dimers

The exact sequences of T1Rs are quite similar among animal species, yet slight

differences still exist.

Artificial sweeteners: Humans enjoy artificial sweeteners, such as aspartame.

Actually, the market for artificial sweeteners is quite large because of weight-gain

concerns. As described above, the sweet receptor is a hetero-dimer of T1R2 and T1R3. It

is interesting to note, however, that mice do not sense the aspartame, although they are

quite sensitive to natural sweeteners such as sucrose as well as other types of

sweeteners, such as saccharin. When murine T1R2 is replaced by human T1R2, mice

become able to sense the aspartame, proving that the site responsible for the perception

of aspartame is located in the sequence of T1R2 which are different between mice and

humans (Nelson et al., 2002)

Thinking about species differences, we can imagine that other animals, but not

humans, might have been enjoying new taste which humans have never even dreamed of.

Cats: Animals belonging to the feline family, such as cats, lions, and tigers, lack

the ability to sense sweetness. Dogs, on the contrary, crave sweet food. This

phenomenon had intrigued researchers over the years. Recently, Lee et al. (2005) proved

that a portion of feline T1R2 is different from that of animals that sense sweetness (Li et al., 2005)

Localization of T1Rs and T2Rs

Various types of T1Rs can co-localize in a single taste cell. Various types of T2Rs

can similarly co-localize in a single taste cell. T1Rs and T2Rs, however, can never co-

localize within a single cell. This means that while there are cells responsible for sensing



66

various types of sweetness, and the cells responsible for sensing various types of

bitterness, but there are no cells sensing both sweetness and bitterness at the same time.

In our experience of cooking, we know that taste elements affect each other. For

example, when Japanese people cook “oshiruko”, sweetened beans, a traditional

Japanese sweet, chefs add small amount of salt into a pan when they cook beans with a

large amount of sugar. The small amount of salt “enhances” the sweetness of

the oshiruko. This enhancement is not taken place in a single taste cell, because the cells

with sweetness receptors and the cells with saltiness receptors are different cells than

each other. Interestingly, the taste cells with T1Rs and the cells with T2Rs can co-exist in

a single taste bud, enabling the possibility that the mutual affection of different tastes

might be taken place in taste buds. Alternatively, it may take place in the brain when

the brain processes the taste information.

Sensitivity to Taste Substances and Localization of the Receptors:

Taste papillae: Taste receptors are localized on the apical cellular surface of taste

cells in taste buds, which are localized in taste papillae. The taste papillae are widely

distributed on the tongue and oral cavity. There are five types of papillae: fungiform,

circumvallate and foliate papillae on the tongue, papillae on the soft palate of the oral

cavity, and papillae on the epiglottis. Millar et al. (1986) counted the numbers of taste

buds on human tongue tips and reported that it averaged 116 buds/cm2 (Miller, 1986)

Solitary some buds were reported even in the trachea (Tizzano et al., 2006)

Different sensitivity to taste in different portions of the tongue: Kieshow

(1894)’s pioneer work revealed that the sensitivities for various taste elements differ

among portions of the tongue (Kugino et al., 1997)

Since then, a lot of research has been conducted to clarify which part of the

tongue/oral cavity is responsible for which type of taste. The tip of the tongue is most

sensitive to sweetness and saltiness, the lateral parts of the tongue are most sensitive to

sourness, and the posterior portions of the tongue, close to the throat, are most sensitive

to bitterness. The recent progress of taste receptor research has cast a new light on them.

Localization of T1Rs in papillae: The distribution and densities of taste receptors

are different depending on their location on the tongue and oral cavity (Nelson et al., 2001)

T1R1: T1R1s are abundantly distributed in the fungiform and palate papillae, while

rarely distributed in the circumvallate and foliate papillae. T1R2: T1R2s are abundantly

distributed in the circumvallate and foliate papillae, small in number in the palate, and rare

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»·è 4 ©ºÑº·è 1 Á¡ÃÒ¤Á - ÁÔ¶Ø¹ÒÂ¹ 2552

in the fungiform papillae. T1R3: T1R3s are ubiquitously distributed in all four types of

papillae.

Their results suggest that if umami is sensed by hetero-dimers of T1R1 and T1R3,

umami should be perceived by mainly by fungiform and palate papillae. Moreover, their

results suggest that if sweetness is sensed by hetero-dimers of T1R2 and T1R3,

sweetness should be perceived by mainly by circumvallate and foliate papillae, which are

localized in posterior portions of the tongue close to the throat.

These results suggest the following: (1) if umami molecules bind to the hetero-

dimers of T1R1 and T1R3, umami should be mainly sensed by the fungiform and palate

papillae; and (2) if sweetness molecules bind to the hetero-dimers of T1R2 and T1R3,

sweetness should be mainly sensed by the circumvallate and foliate papillae, which are

localized in the posterior portions of the tongue. However, these results are inconsistent

with sensitivity tests on human’s tongues investigated by Kieshow, Collings, and others.

When we eat ice cream, for example, we lick the ice cream with the tip of the tongue,

thus maximizing the sense of sweetness. It means that we know that the tip of our

tongues is quite sensitive to sweetness. There are condensed distributions of fungiform

papillae on the tip of the tongue. Nevertheless, Nelson et al. (2001) suggested that the

heterodimers of T1R2 and T1R3 are only seldomly observed in fungiform papillae. Some

more research should be conducted to solve these conflicts.

In Conclusion:

In this review, we summarized the recent progress on taste research focusing

on taste receptors, in particular T1R and T2R families. We described five taste elements,

sweetness, bitterness, sourness, saltiness and umami. The receptors of sweetness,

bitterness and umami are seven transmembrane proteins coupled with G-proteins, while

receptors of sourness and saltiness are ion channels. Sweetness receptors were

categorized as T1Rs and bitterness receptors as T2Rs. Heterodimers of T1R1 and T1R3

are responsible for umami, and heterodimers of T1R2 and T1R3 are for sweetness. Their

localizations and relation to sensitivities to various taste elements have been also

discussed.



68

Bovine taste buds stained with anti-alpha gustducin antibody

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taste and olfaction. (Chemical Senses, vol. 1) Marcel Dekker Inc.

Chandrashekar, J., Mueller, K.L., Hoon, M.A., Adler, E., Feng, L., Guo, W., Zuker, C.S. and

Ryba, N.J. 2000. T2Rs function as bitter taste receptors. Cell 100(6): 703-11.

Filer, L.J.Jr., Garattini, S., Kare, M.R., Reynolds, W.A. and Wurtman, R.J. 1979. Glutamic

Acid: In: “Advances in biochemistry and physiology”, Raven Press.

Kitagawa, M., Kusakabe, Y., Miura, H., Ninomiya, Y. and Hino, A. 2001. Molecular genetic

identification of a candidate receptor gene for sweet taste. Biochem Biophys Res

Commun. 283(1): 236-42.

Kugino, K., Kaneko, M., Akiyoshi, T. and Mizunuma, T.1997. Studies on the taste perceptive

threshold for 4 basic taste qualities at various sites of lingual surface. Fukuoka

Igaku Zasshi 88(10): 331-6.

Li, X., Li, W., Wang, H., Cao, J., Maehashi, K., Huang, L., Bachmanov, A.A., Reed, D.R.,

Legrand-Defretin, V., Beauchamp, G..K. and Brand, J.G.. 2005. Pseudogenization of a

sweet-receptor gene accounts for cats’ indifference toward sugar. PLoS Genet.

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Margolskee, R.F. 2002. Molecular mechanisms of bitter and sweet taste transduction. J

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Max, M., Shanker, Y.G., Huang, L., Rong, M., Liu, Z., Campagne, F., Weinstein, H. and

Damak, S., Margolskee, R.F. 2001. Tas1r3, encoding a new candidate taste

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Figure 1

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Miller, I.J.Jr. 1986. Variation in human fungiform taste bud densities among regions and

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Mammalian sweet taste receptors. Cell 106(3):381-90.

Nelson, G., Chandrashekar, J., Hoon, M.A., Feng, L., Zhao, G., Ryba, N.J. and Zuker, C.S.

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Yamaguchi, S. 1991. Basic properties of umami and effects on humans. Physiol Behav.

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70

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fixation

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µÃÐกÒÃÈÑก´Ôì á¾ä¸Ê§ áÅÐ¾ÔäÅ¾Ã à¨µÔÂÇÃÃ³. 2550. ÊÀÒÇÐâÃ¤ºÃÙà«ÅâÅ«ÕÊã¹á¾Ðã¹ÀÒ¤µÐÇÑ¹µก¢Í§»ÃÐà·Èä·Â. Thai-NIAH eJournal : ISSN 1905-5048, Available from :http//www.dld.go.th/niah, V2 N2(September-December 2007)

¸ÕÃÈÑก´Ôì ªÑก¹Ó ¸Ã³Ô¹·Ãì ¸ÒÃÐÇÑ¹ ·ÇÕÊÔ·¸Ôì ¹ÔÅÇÔÊØ·¸Ôì ¨ÃÔÂÒ ¹ÃÒ¸Ô»ÀÑ·Ã áÅÐÊØ¸Õ¾Ã áÊ§ÃÑµ¹ì. 2548.ÃÒÂ§Ò¹àº×éÍ§µé¹กÒÃÃÐºÒ´¢Í§âÃ¤ºÃÙà«ÅâÅ«ÕÊã¹ÊÒÁ¨Ñ§ËÇÑ´ÀÒ¤ãµé. ÃÒÂ§Ò¹à½éÒÃÐÇÑ§·Ò§ÃÐºÒ´ÇÔ·ÂÒ»ÃÐ¨ÓÊÑ»´ÒËì.»Õ·Õè 36 ©ºÑº·Õè 44. Ë¹éÒ 770-772.

Á¹ÂÒ àÍก·ÑµÂì. 2547. �กÒÃ¤Çº¤ØÁ»éÍ§กÑ¹âÃ¤ºÃÙà«ÅâÅ«ÕÊ�, ¨´ËÁÒÂ¢èÒÇÊ¶ÒºÑ¹ÊØ¢ÀÒ¾ÊÑµÇìáËè§ªÒµÔ.»Õ·Õè 4 ©ºÑº·Õè 1 »ÃÐ¨Óà ×Í¹µØÅÒ¤Á.

ÊÓ¹ÑกÃÐºÒ´ÇÔ·ÂÒ กÃÁ¤Çº¤ØÁâÃ¤ กÃÐ·ÃÇ§ÊÒ¸ÒÃ³ÊØ¢. 2546. กÒÃÃÐºÒ´¢Í§âÃ¤ºÃÙà«ÅâÅ«ÕÊâÃ¤µÔ´àª×éÍ·ÕèÍÒ¨à»ç¹»ÑËÒãËÁè¢Í§»ÃÐà·Èä·Â,ÃÒÂ§Ò¹à½éÒÃÐÇÑ§·Ò§ÃÐºÒ´ÇÔ·ÂÒ. 34(27): 496-498.

ÊÓ¹Ñก¤Çº¤ØÁ »éÍ§กÑ¹ áÅÐºÓºÑ´âÃ¤ÊÑµÇì กÃÁ»ÈØÊÑµÇì กÃÐ·ÃÇ§àกÉµÃáÅÐÊËกÃ³ì. 2548. ¤ÙèÁ×ÍàกÉµÃกÃã¹กÒÃ»éÍ§กÑ¹กÓ Ñ âÃ¤á·é§µÔ µèÍ ËÃ×ÍâÃ¤ºÃÙà«ÅâÅ«ÕÊã¹á¾Ð-áกÐ. กÃØ§à·¾Ï. 29 ¹.

ÊèÇ¹»éÍ§กÑ¹áÅÐºÓºÑ´âÃ¤ÊÑµÇì ÊÓ¹ÑกÊØ¢ÈÒÊµÃìÊÑµÇìáÅÐÊØ¢Í¹ÒÁÑÂ·Õè 9 กÃÁ»ÈØÊÑµÇì. 2548. àÍกÊÒÃà¼Âá¾Ãè

ÍÑ§É³Ò ÂÈ»ÑÒ áÅÐ¸ÕÃÈÑก´Ôì ªÑก¹Ó. 2549. ÃÒÂ§Ò¹กÒÃÃÐºÒ´¢Í§âÃ¤ºÃÙà«ÅâÅ«ÕÊ¨Ñ§ËÇÑ´àÅÂ,

ÃÒÂ§Ò¹à½éÒÃÐÇÑ§·Ò§ÃÐºÒ´ÇÔ·ÂÒ »Õ·Õè 37 ©ºÑº·Õè 3. Ë¹éÒ 41-49.

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infected cattle, sheep, goats and camels by PCR. Veterinary Journal. 163 : 299-305.Waghela, S. 1978. Serological response of adult goats infected with live Brucella Melitensis.

British Veterinary Journal .134 : 565-571.



78

Brucellosis in Goats

Sommai Kaopiew1, # and Komkrich Pimpukdee1

Abstract

Goat brucellosis is an important animal disease because it is a zoonosis and worldwide

finding. Goat brucellosis is caused by bacteria characterized as a gram-negative coccobacillus

(round bar and small size), and no spore, called Brucella melitensis. The risk factors of

brucellosis infection are contact with secretion contaminated with Brucellosis melitensis, such as

placenta and uterine secretion, contact with infectious animals, mating, and drinking milk and

consuming meat from infected animals, which are without cooking process by heat. The most

significant signs in goats are late abortion at last two months of gestation, mastitis, arthritis,

lameness, probably nervous signs, orchitis and testicular swelling in male goats. Symptoms of

infected human are febrile, weakness, weight loss, muscular pain and arthritis, splenomegaly,

and orchitis in men. In Thailand, there were reports of goat brucellosis epidemic in some areas;

north-eastern, western, and three provinces at border of the southern part of Thailand. There

are also reported that the brucellosis can transmit to human.

Keywords: Brucellosis, Goats

1Department of Veterinary Public Health, Faculty of Veterinary Medicine, Khonkaen University, Khon Kaen, 40002#Corresponding author : E-mail : [email protected]

79


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¤ÓµÍº»ÃÔÈ¹Ò-ÊÑµÇì»èÇÂ

¤ÓµÍºกÒÃÇÔ¹Ô ©ÑÂ·ÓกÒÃàกçºµÑÇÍÂèÒ§¨ÒกกéÍ¹à¹×éÍ´éÇÂÇÔ¸Õ Fine needle aspiration (FNA) à¾×èÍÊè§µÃÇ¨ÇÔ¹Ô¨©ÑÂ·Ò§

à«ÅÅìÇÔ·ÂÒ (Cytology) «Öè§¼ÅกÒÃµÃÇ¨¾ºÇèÒà»ç¹à¹×éÍ§Íกª¹Ô´ Transmissible venereal tumor (TVT)ËÃ×Í Venereal granuloma (VG)

กÒÃÃÑกÉÒ·ÓกÒÃÃÑกÉÒâ´ÂกÒÃ©Õ´ Vincristin sulfate ¢¹Ò´ 0.025 mg/BW kg à¢éÒ·Ò§ËÅÍ´àÅ×Í´´Ó

ÊÑ»´ÒËìÅÐ 1 ¤ÃÑé§ à»ç¹àÇÅÒ 6 ÊÑ»´ÒËì ÃèÇÁกÑºกÒÃãËéÂÒºÓÃØ§µÑºª¹Ô´กÔ¹ «Öè§¼ÅกÒÃÃÑกÉÒ¾ºÇèÒกéÍ¹à¹×éÍ§ÍกÁÕ¢¹Ò´àÅçกÅ§µÒÁÅÓ´Ñº ´Ñ§áÊ´§ã¹ÀÒ¾ A � E ´éÒ¹ÅèÒ§¹Õé «Öè§áÊ´§à»ç¹ÊÑ»´ÒËìËÅÑ§¨ÒกàÃÔèÁกÒÃÃÑกÉÒ

ÀÒ¾ A : ÊÑ»´ÒËì·Õè 2 ÀÒ¾ B : ÊÑ»´ÒËì·Õè 3 ÀÒ¾ C : ÊÑ»´ÒËì·Õè 4

ÀÒ¾ D : ÊÑ»´ÒËì·Õè 5 ÀÒ¾ E : ÊÑ»´ÒËì·Õè 6



80

Canine transmissible venereal tumor

Canine transmissible venereal tumor (CTVT) ÁÕª× èÍ¾éÍ§ ¤×Í Venereal granuloma,Transmissible lymphosarcoma, Sticker’s tumor ËÃ×Í Infectious sarcoma «Öè§à»ç¹à¹×éÍ§Íก·ÕèÁÑก¾ºä´éºèÍÂ·Õ èºÃÔàÇ³ÀÒÂã¹áÅÐÀÒÂ¹Íก¢Í§ÍÇÑÂÇÐÊ×º¾Ñ¹¸Ø ì â´Â¾ºä´é¹éÍÂ·Õ èºÃÔàÇ³ÍÇÑÂÇÐÍ× è¹æ ÊÒÁÒÃ¶µÔ´µèÍâ´ÂกÒÃ Transplant ä»·ÕèÍÇÑÂÇÐÊ×º¾Ñ¹¸ØìÀÒÂ¹Íก¨ÒกกÒÃ¼ÊÁ¾Ñ¹¸Øì «Öè§Êè§¼ÅãËéàกÔ´¼ÔÇË¹Ñ§ËÃ×ÍàÂ×èÍàÁ×ÍกÅÍกËÅØ´áÅÐµÔ´à¹×éÍ§Íกª¹Ô´¹Õéä´é TVT ÊÒÁÒÃ¶àกÔ´¢Öé¹ä´éกÑºÊØ¹Ñ¢·ØกÊÒÂ¾Ñ¹¸Øì â´Â¾ºä´éºèÍÂã¹ÊØ¹Ñ¢ÍÒÂØ¹éÍÂ áÅÐÁÑก¾ºã¹ÊØ¹Ñ¢à¾ÈàÁÕÂÁÒกกÇèÒà¾È¼Ùé ¢¹Ò´¢Í§ TVT ÍÒ¨ÁÕ¢¹Ò´àÅçกÁÒก ËÃ×Íà»ç¹ÅÑกÉ³ÐกéÍ¹à¹×éÍ§Íก¢¹Ò´àÅçก¨¹¶Ö§ÁÕ¢¹Ò´ãËè ¢¹Ò´àÊé¹¼èÒÈÙ¹ÂìกÅÒ§ 10 à«¹µÔàÁµÃ ÅÑกÉ³ÐกÒÃâµ¢Í§กéÍ¹à¹×éÍ§Íก ÍÒ¨¢ÂÒÂ¢¹Ò´¨ÒกกéÍ¹à´ÕèÂÇæ ¨¹à»ç¹ Nodule ËÃ×ÍÃÇÁµÑÇกÑ¹à»ç¹กÅØèÁàกÔ´à»ç¹ÅÑกÉ³Ð¤ÅéÒÂ´ÍกกÃÐËÅèÓ (Cauliflower-like mass)

กÒÃàกÔ´ TVT ·ÕèÍÇÑÂÇÐÍ×è¹¢Í§ÃèÒ§กÒÂ¹ÍกàË¹×Í¨ÒกÍÇÑÂÇÐÊ×º¾Ñ¹¸Øì (Extragenital TVT, ETVT)ÍÒ¨àกÔ´¢Öé¹ä´é·ÕèºÃÔàÇ³¼ÔÇË¹Ñ§ àÂ×èÍàÁ×Íก¢Í§¨ÁÙกáÅÐµÒ ÊØ¹Ñ¢·Õè¾ºà¹×éÍ§Íก TVT ·ÕèºÃÔàÇ³ÍÇÑÂÇÐàËÅèÒ¹ÕéÍÒ¨µÃÇ¨äÁè¾º TVT ã¹ÃÐººÊ×º¾Ñ¹ Øìกçä é à¤ÂÁÕÃÒÂ§Ò¹ÇèÒ TVT ÊÒÁÒÃ¶á¾ÃèกÃÐ¨ÒÂ (Metastasis) ä»ÂÑ§ÊÁÍ§áÅÐµÒä é â´Â¾ºÇèÒÍÒกÒÃ¢Í§ Ocular TVT ¨ÐÁÕÍÒกÒÃ ¤×Í à¹×éÍ§Íก¨Ð protrude ¨ÒกกÃÐ¨กµÒ(Cornea) «Öè§ÊÒàËµØ¢Í§กÒÃ¾º TVT ¹ÍกÃÐººÊ×º¾Ñ¹¸Øì ÍÒ¨¨ÐàกÔ´¨ÒกกÒÃ Transplantation ËÃ×ÍàกÔ â´ÂµÃ§¨Òก éÒ¹ã¹ Iris ËÃ×Í Ciliary body (àกÉก¹ก, 2548)

กÒÃÃÑกÉÒ TVT ÊÒÁÒÃ¶·Óä´éËÅÒÂÇÔ¸ Õ ¤ ×Í â´ÂกÒÃ¼èÒµÑ´àÍÒกéÍ¹à¹× éÍ§ÍกÍÍก à¤ÁÕºÓºÑ´ (Chemotherapy) ÃÑ§ÊÕºÓºÑ´ (Radiotherapy) ÃÇÁ¶Ö§ Immunotherapy «Öè§ã¹·Ò§¤ÅÔ¹Ôก¹ÔÂÁãªéÇÔ¸Õà¤ÁÕºÓºÑ´ã¹กÒÃÃÑกÉÒ à¹×èÍ§¨Òก¼ÅกÒÃµÍºÊ¹Í§·Õè´Õ ÊÐ´Çก §èÒÂ áÅÐàËÁÒÐÊÓËÃÑºÃÒÂ·ÕèÁÕกÒÃá¾ÃèกÃÐ¨ÒÂ¢Í§à¹×éÍ§Íก â´Âà¤ÁÕºÓºÑ´·Õè¹ÔÂÁãªé ¤×Í Vincristine ¢¹Ò´·Õèãªé 0.025 mg/BW kg ·Ò§ËÅÍ´àÅ×Í´´Ó ËÃ×Íã¹¢¹Ò´ 0.50 � 0.75 mg/m2 «Öè§©Õ´ÊÑ»´ÒËìÅÐ 1 ¤ÃÑé§ à»ç¹àÇÅÒ 2 � 7 ÊÑ»´ÒËìÃèÇÁกÑºกÒÃµÃÇ¨·Ò§âÅËÔµÇÔ·ÂÒ¤èÒàÅ×Í´ (Complete blood count; CBC) ÃèÇÁกÑºกÒÃµÃÇ¨¤èÒªÕÇà¤ÁÕ¢Í§àÅ×Í´ ¤×Í SGPT

àกÉก¹ก ÈÔÃÔ¹ÄÁÔµÃ. 2548. Transmissible Venereal Tumor. ÇÔ·ÂÒÃÐººÊ×º¾Ñ¹¸ØìÊØ¹Ñ¢à¾ÈàÁÕÂ.ÊÓ¹Ñก¾ÔÁ¾ìâ»Ã¾ÃÔé¹¾ÃÕà¾ÃÊ. กÃØ§à·¾ÁËÒ¹¤Ã. Ë¹éÒ 143 �149.

Boscos, C.M. and Ververidis, H.N. 2004. Canine TVT : Clinical finding, diagnosis and treatment.

In Proc. 29th World Congress of World Small Animal Veterinary Association (WSAVA),

6-9 October 2004, Rhodes, Greece.

Mello Martins, M.I., Ferreira de Souza, F. and Gobello, C. 2005. The canine transmissible

venereal tumor : Etiology, pathology, diagnosis and treatment, Recent advance in small

animal reproduction : International veterinary information service (www.ivis.org). Ithaca,

New York, USA.


81


»·è 4 ©ºÑº·è 1 Á¡ÃÒ¤Á - ÁÔ¶Ø¹ÒÂ¹ 2552

¤ÓµÍºWhat’s your cytological diagnosis?

¼ÅกÒÃµÃÇ¨·Ò§à«ÅÅìÇÔ·ÂÒ ¾º squamous epithelial cells ÃèÇÁกÑº¹ÔÇâ·Ã¿ÔÇ¨Ó¹Ç¹ÁÒกà«ÅÅìàËÅèÒ¹Õ éÍÂÙ èµ Ô´กÑ¹ÍÂèÒ§ËÅÇÁæáÅÐºÒ§à«ÅÅìà»ç¹à«ÅÅìà´Õ èÂÇ ´Ñ§·Õ èáÊ´§äÇéã¹ÃÙ»·Õ è1áÅÐ 2ÅÑกÉ³Ðà»ç¹à«ÅÅì¢¹Ò´ãËèกÇèÒàÁç´àÅ×Í´á´§ (ËÑÇÅÙกÈÃªÕ é) »ÃÐÁÒ³ 5-10 à·èÒ ÃÙ»ÃèÒ§àËÅÕ èÂÁ¢¹Ò´¢Í§à«ÅÅìáµกµèÒ§กÑ¹ (anisocytosis) ¢Íºà¢µ¢Í§à«ÅÅìàËç¹ä´éªÑ´à¨¹¹ÔÇà¤ÅÕÂÊÁÕÃÙ»ÃèÒ§กÅÁ¢¹Ò´áµกµèÒ§กÑ¹ (anisokaryosis) ÁÕË¹Ö è§¶ Ö§ÊÍ§ÍÑ¹ (binucleated) áÊ´§äÇéã¹ÃÙ»·Õ è1µÓáË¹è§àÍÕÂ§ä»¢éÒ§ã´¢éÒ§Ë¹Ö è§ áÅÐÁÕ¹ÔÇ¤ÅÕâÍÅÑÊËÅÒÂกéÍ¹ÍÂÙ èã¹¹ÔÇà¤ÅÕÂÊ ¾ºÍÑµÃÒÊèÇ¹¢Í§¹ÔÇà¤ÅÕÂÊµèÍä«âµ¾ÅÒÊÁ (N:C ratio) ÊÙ§ ä«âµ¾ÅÒÊÁµÔ´ÊÕ¹éÓà§Ô¹à¢éÁ ÀÒÂã¹ºÃÃ¨Ø´éÇÂáÇ¤¤ÔÇâÍÅ¨Ó¹Ç¹ÁÒก

Cytological diagnosis: Epithelial tumor highly suggestive for squamous cell carcinoma.

ÇÔ ÒÃ³ì ¼ÅกÒÃµÃÇ¨ªÔé¹à¹×éÍ·Ò§ ØÅ¾ÂÒ ÔÇÔ·ÂÒ ¾ºà»ç¹ÁÐàÃç§ª¹Ô squamous cell carcinoma

กÒÃÇÔ¹Ô¨©ÑÂ·Ò§à«ÅÅìÇÔ·ÂÒ¢Í§ squamous cell carcinoma µéÍ§ÇÔ¹Ô¨©ÑÂáÂกâÃ¤ ÍÍก¨ÒกกÅØèÁ¢Í§epithelial tumor Í×è¹æ àªè¹ Basal cell tumor, Hair follicle tumor, Intracutaneous conifying epithelioma

à»ç¹µé¹ â´ÂÍÒÈÑÂËÅÑกกÒÃ´Ù criteria ¢Í§à«ÅÅìà¹×éÍ§Íก·ÕèÁÑกÁÕËÅÒÂÊèÇ¹»ÃÐกÍºกÑ¹ä´éáกè à«ÅÅì·ÕèÁÕ¢¹Ò´áµกµèÒ§กÑ¹ กÒÃ¾ºà«ÅÅì·ÕèÁÕËÅÒÂ¹ÔÇà¤ÅÕÂÊ ÁÕâ¤ÃÁÒµÔ¹ËÂÒºáÅÐµÔ´ÊÕà¢éÁ ÁÕËÅÒÂ¹ÔÇ¤ÅÕâÍÅÑÊáÅÐÁÕÍÑµÃÒÊèÇ¹¹ÔÇà¤ÅÕÂÊµèÍä«âµ¾ÅÒÊÁÊÙ§ à»ç¹µé¹

à¹×éÍ§Íกª¹Ô´ squamous cell carcinoma ¹Ñé¹¾ºä´éºèÍÂã¹ªèÍ§»Òก,¨ÁÙก,ãºËÙ ÃÇÁ¶Ö§µÒÁ»ÅÒÂ¢Ò¢Í§ÊØ¹Ñ¢áÅÐáÁÇ áµè¾ºä´é¹éÍÂã¹ÊÑµÇìÊÒÂ¾Ñ¹¸Ø ìÍ× è¹æ (Carton and McGavin,1995). áµèกçÁÕÃÒÂ§Ò¹ÊÑµÇì»èÇÂ¨ÒกกÒÃ·Õè¾º squamous cell carcinoma ·Õè cheek pouch ã¹Ë¹ÙáÎÁÊàµÍÃìá¤ÃÐ(Martorell et al.,2005) ¶Ö§áÁéÇèÒ ¨ÐµéÍ§´ÙกÒÃá¾ÃèกÃÐ¨ÒÂä»·Õ èµèÍÁ¹éÓàËÅ×Í§´éÇÂã¹กÃ³Õ¢Í§cheek pouch carcinoma (Ohtake et al., 1993) áµèã¹กÃ³ÕÊÑµÇì»èÇÂµÑÇ¹Õé ÂÑ§µÃÇ¨äÁè¾ºกÒÃá¾ÃèกÃÐ¨ÒÂä»µÒÁ µèÍÁ¹éÓàËÅ×Í§µèÒ§æ

กÒÃµÔ´µÒÁ¼ÅกÒÃÃÑกÉÒ 3 à´×Í¹ËÅÑ§กÒÃ¼èÒµÑ´ ÂÑ§äÁè¾ºกÒÃàกÔ´¢Öé¹ãËÁè¢Í§à¹×éÍ§Íก áµè·Ñé§¹ÕéÊÑµÇá¾·Âìä éá¹Ð¹ÓãËéà éÒ¢Í§¹ÓÊÑµÇìÁÒÃÑºกÒÃµÃÇ¨ÍÂèÒ§µèÍà¹×èÍ§µèÍä»



82

ºÃÃ³Ò¹ØกÃÁ

Baker, R. and Lumsden,J.H. 1999. The skin. In: Color atlas of cytology of the dog and cat. R. Baker

and J.H. Lumsden (eds.) St.Louis: Mosby, Yearbook. 42-43.

Carton, WW and McGavin, MD (1995). Thomson’s special veterinary pathology. 2nd Edn., St. Louis,

Missouri, Mosby-year Book. P:10.

Martorell, J; Foundevila, D and Ramis, A(2005). Spontaneous squamous cell carcinoma of the cheek

pouch in two dwarf hamsters (Phodopus sungorus). Vet Rec., 14: 650-651.

Ohtake, K; Shingaki, S and Nakajima, T(1993). Histologic study on the metastatic process in the

experimental model of lymph node metastasis. Oral Surg. Oral Med. Oral Path.,75:472-478

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