feed analysis

Feed analysis

2

Panreac has the analytical reagents with sufficient purity to perform the following determinations, pursuant to the provisions of the regulation:

Composition of materials for feed and compound feed

Level of additives authorised

Control of undesirable substances

Determination of constituents of animal origin

Control of the illegal presence of unauthorised additives

The control of products destined for use as animal feed is of great importance, because feed production is directly linked to the quality of meat products, milk and dairy products, and consequently to human health. Commission Regulation (EC) No 152/2009, of 27 January 2009, lays down the methods of sampling and analysis for the official control of feed.

Feed analysis

3

Listeria monocytogenes, Escherichia coli O157:H7 and Clostridium sp. In the absence of specific regulations for microbiological tests for feed, the same methods used for the food industry can be applied.

The Panreac CULTIMED culture media allows these determinations to be made.

In addition to chemical analyses, microbiological tests are also recommended.

The Panel of Biological Hazards (BIOHAZ) prepared a report that identified Salmonella spp. as the primary risk of microbial contamination in feed for food producing animals. Other risks of lesser importance are

4

Analysis Method Panreac reagent recommended Code

Crude Protein Kjeldahl Acetanilide (Reag. USP) PA 121005

Boric Acid PA-ACS-ISO 131015

Ammonia Fixative Solution 1% RV 283334 (1)

Boric Acid solution 3% RV 282928

Boric Acid solution 4% RV 282222

Bromocresol Green PA-ACS 131759

Buffer Solution pH 4,00 ±0,02 (20°C) ST 272168

Buffer Solution pH 7,00 ±0,02 (20°C) ST 272170

Copper(II) Oxide PRS 141269

Copper(II) Sulphate 5-hydrate (Reag. Ph. Eur.) PA-ACS-ISO 131270

Ethanol 96% v/v PA 121085

Hydrochloric Acid 0,1 mol/l (0,1N) SV 181023

Indicator 4,8, Mixed (Methyl Red-Bromocresol Green) RV 283303

Kjeldahl Catalyst (Cu) (6,25% in CuSO4.5H2O) tablets RE 174428

Methyl Red PA-ACS 131617

Methyl Red solution 0,1% RV 281618

Potassium Sulphate (Reag. Ph. Eur.) PA-ACS-ISO 131532

Pumice Stone granules QP 211835

Saccharose PA-ACS 131621

Sodium Hydroxide solution 40% w/v PA 121593

Sodium Hydroxide solution 40% w/w RE 171220

Sodium Hydroxide 0,1 mol/l (0,1N) SV 181693


Sulphuric Acid 98% RE 173163

Sulphuric Acid 0,05 mol/l (0,1N) SV 181061



Urea Photometry Acetic Acid glacial (Reag. Ph. Eur.) PA-ACS-ISO 131008

Carrez's Reagent I RE 173355

Carrez's Reagent II RE 173356

Charcoal Activated powder PA 121237

4-(Dimethylamino) Benzaldehyde PA 121293


Hydrochloric Acid 37% PA-ACS-ISO 131020

Potassium Hexacyanoferrate(II) 3-hydrate (Reag. Ph. Eur.) PA-ACS-ISO 131505

Zinc Acetate 2-hydrate (Reag. USP, Ph. Eur.) PA-ACS 131775

We demonstrate below the analysis methods provided for in Regulation (EC) No 152/2009 alongside the recommended Panreac reagent.

(1) For automatic analysis, prepared solution of boric acid and indicator.

METHODS OF ANALYSIS TO CONTROL THECOMPOSITION OF FEED MATERIALS AND COMPOUND FEED

Feed analysis

5


Volatile Nitrogenous Bases

Microdiffusion Boric Acid PA-ACS-ISO 131015

Bromocresol Green PA-ACS 131759


Indicator 4,8, Mixed (Methyl Red-Bromocresol Green) RV 283303


Potassium Carbonate PA 121490


Trichloroacetic Acid solution 20% w/v DC 252373

Distillation Ethanol 96% v/v PA 121085

Magnesium Oxide light (RFE, BP, Ph. Eur.) PRS-CODEX 141276



Silicone antifoaming liquid (AQ) QP 216241




Amino Acids (except Tryptophan)

Ionic Chromatography and photometric detection

Formic Acid 98% PA-ACS 131030


Hydrogen Peroxide 33% w/v (110 vol.) PA-ACS-ISO 131077

Ninhydrin PA-ACS 132362

1-Octanol (Reag. USP) PA-ACS 133386

Petroleum Ether 40-60°C PA-ACS-ISO 131315

Phenol crystallized (detached crystals) PA-ACS 134852

di-Phosphorus penta-Oxide PRS 141154

Sodium Chloride PA-ACS-ISO 131659

Sodium Chloride PA 121659

tri-Sodium Citrate 2-hydrate PA-ACS 131655

Sodium Disulphite PA-ACS 131698

Sodium Hydroxide pellets PA-ACS-ISO 131687

Sodium Hydroxide 1 mol/l (1N) SV 181691

5-Sulphosalicylic Acid 2-hydrate (Reag. Ph. Eur.) PA-ACS 132838

Tryptophan HPLC with fluorescence detection

Acetic Acid glacial (Reag. Ph. Eur.) PA-ACS-ISO 131008

Barium Hydroxide 8-hydrate (Reag. Ph. Eur.) PA-ACS-ISO 131188

Ethanolamine PA-ACS 131924



Hydrochloric Acid 1 mol/l (1N) SV 181021

Methanol (UV-IR-HPLC-HPLC isocratic) PAI-ACS 361091


ortho-Phosphoric Acid 85% PA-ACS-ISO 131032

di-Phosphorus penta-Oxide PRS 141154


Sodium Hydroxide 1 mol/l (1N) SV 181691

1,1,1-Trichloro-2-Methyl-2-Propanol 1/2-hydrate (RFE, BP, Ph. Eur.) PRS-CODEX 145300

6


Crude Oilsand Fats

Extraction with petroleum ether


Sodium Sulphate anhydrous (Reag. USP) PA-ACS-ISO 131716

Extraction with hydrochloric acid

Celite Hyflo Super Cel ® RE 175772


Crude Fibre Weight losson ignition

Acetone (Reag. Ph. Eur.) PA-ACS-ISO 131007

Celite Hyflo Super Cel ® RE 175772


1-Octanol (Reag. USP) PA-ACS 133386


Potassium Hydroxide 0,23 mol/l (0,23N) SV 183354

Sodium Hydroxide 0,313 mol/l (0,313N) SV 183337 (2)

Sulphuric Acid 0,1275 mol/l (0,255N) SV 183335 (2)

Sulphuric Acid 0,13 mol/l (0,26N) RE 176191

Detergent Acid Solution RE 175055 (3)

Detergent Neutral Solution RE 175054 (3)

Sugar Luff-Schoorl Acetic Acid glacial (Reag. Ph. Eur.) PA-ACS-ISO 131008



Citric Acid 1-hydrate PA-ACS-ISO 131018

Copper(II) Sulphate 5-hydrate (Reag. Ph. Eur.) PA-ACS-ISO 131270

Ethanol absolute PA-ACS-ISO 131086


Hydrochloric Acid 4 mol/l Prepare with 131020

Luff-Schoorl's Reagent RE 172174

3-Methyl-1-Butanol (Reag. Ph. Eur.) PA-ACS 131079

Methyl Orange solution 0,1% RV 281432


Potassium Iodide PA 121542

Potassium Iodide solution 30% w/v VINIKIT 624572

Pumice Stone granules QP 211835

Sodium Carbonate anhydrous (Reag. Ph. Eur.) PA-ACS 131648


Sodium Thiosulphate 0,1 mol/l (0,1N) (Reag. USP) SV 181723

Starch from Potato soluble (Reag. USP, Ph. Eur.) PA 121096

Starch solution 1% RV 283146

Sulphuric Acid 3 mol/l Prepare with 131058


Lactose Luff-Schoorl after fermentation Same reagents as sugar determination

Starch Polarimetry Acetic Acid glacial (Reag. Ph. Eur.) PA-ACS-ISO 131008



Ethanol absolute PA-ACS-ISO 131086

Hydrochloric Acid 25% PA-ISO 133378





Feed analysis

(2) Determination by Weende method (3) Determination by Van Soest’s method

7


Vitamin A HPLC with fluorescence detection

2,6-Di-tert-Butyl-4-Methylphenol (RFE, BP, Ph. Eur.) PRS-CODEX 142825


Glycerol PA-ACS-ISO 131339



Phenolphthalein solution 2% RE 172866

Potassium Hydroxide 85% pellets PA 121515

2-Propanol (HPLC-gradient grade-UV-IR) PAI 221090

Sodium L(+)-Ascorbate (USP) PRS-CODEX 143865

Sodium Sulphide x-hydrate QP 211682

Vitamin E HPLC with fluorescence detection

2,6-Di-tert-Butyl-4-Methylphenol (RFE, BP, Ph. Eur.) PRS-CODEX 142825





Phenolphthalein solution 2% RE 172866



Sodium Sulphide x-hydrate QP 211682

METHODS OF ANALYSIS TO CONTROLTHE LEVEL OF AUTHORISED ADDITIVES IN FEED


Ash insoluble in hydrochloric acid

Residue on ignition


Trichloroacetic Acid (Reag. Ph. Eur.) PA-ACS 131067


Carbonates CO2 measurement using Scheibler-Dietrich apparatus

Calcium Carbonate precipitated PA 121212




Total phosphorus Photometry Ammonia 30% (as NH3) PA-ACS 131130

Ammonium Molybdate 4-hydrate (Reag. Ph. Eur.) PA-ACS-ISO 131134

Ammonium meta-Vanadate (Reag. USP, Ph. Eur.) PA-ACS 132352

Calcium Carbonate precipitated PA 121212


Nitric Acid 69% PA-ACS-ISO 131037

Nitric Acid 65% PA-ISO 133255

Phosphorus standard solution P=1,000±0,002 g/l IC 786922

Potassium di-Hydrogen Phosphate PA 121509

Sulphuric Acid 96% PA-ISO 131058

Chlorine from chlorides

Titration by Volhard's method


Acetone (Reag. Ph. Eur.) PA-ACS-ISO 131007

Ammonium Iron(III) Sulphate 12-hydrate (Reag. Ph. Eur.) PA-ACS-ISO 131365

Ammonium Thiocyanate 0,1 mol/l (0,1N) (Reag. USP, Ph.Eur.) SV 181144



Charcoal Activated powder PA 121237

Diethyl Ether stabilized with ~6 ppm of BHT (Reag. Ph. Eur.) PA-ACS-ISO 132770


Nitric Acid 65% PA-ISO 133255


Silver Nitrate 0,1 mol/l (0,1N) SV 181464


8


Trace elements Iron, Copper, Manganese and Zinc

Atomic absorption spectroscopy (AAS)

Copper standard solution Cu=1,000±0,002 g/l AA 313178



Hydrofluoric Acid 48% PA-ACS-ISO 131028

Hydrogen Peroxide 33% w/v (110 vol.) PA-ACS-ISO 131077

Iron standard solution Fe=1,000±0,002 g/l AA 313182

Lanthanum(III) Chloride 7-hydrate PA 122848

Lanthanum(III) Oxide (Reag. Ph. Eur.) PA 122705

Manganese standard solution Mn=1,000±0,002 g/l AA 313185

Sulphuric Acid 96% PA-ISO 131058

Zinc standard solution Zn=1,000±0,002 g/l AA 313193

Halofuginone HPLC with UV detection


Acetonitrile (UV-IR-HPLC-isocratic) PAI-ACS 361881

Ammonium Acetate (Reag. Ph. Eur.) PA-ACS 131114

Ethyl Acetate (UV-IR-HPLC-HPLC preparative) PAI-ACS 361318

Ethylenediaminetetraacetic Acid Disodium Salt 2-hydrate (Reag. Ph. Eur.) PA-ACS 131669




Silver Nitrate PA-ACS-ISO 131459

Silver Nitrate 0,1 mol/l (0,1N) SV 181464


Sodium Carbonate anhydrous (Reag. Ph. Eur.) PA-ACS 131648

Sodium Chloride PA 121659

Water (UV-HPLC) PAI-ACS 361074

Robenidine HPLC with UV detection





di-Sodium Hydrogen Phosphate anhydrous (Reag. Ph. Eur.) PA-ACS 131679

di-Sodium Hydrogen Phosphate 2-hydrate PA 122507

di-Sodium Hydrogen Phosphate 12-hydrate PA-ISO 131678


Diclazuril Gradient HPLC with UV detection

Acetonitrile (HPLC-gradient grade) PAI-ACS 221881

Ammonium Acetate (Reag. Ph. Eur.) PA-ACS 131114

N,N-Dimethylformamide (UV-IR-HPLC-GPC) PAI-ACS 361785


Methanol (HPLC-gradient grade) PAI-ACS 221091

Tetrabutylammonium Hydrogen Sulphate (HPLC) PAI 363622


Lasalocid sodium

HPLC with spectrofluoro-metric detection



ortho-Phosphoric Acid 85% PA-ACS-ISO 131032



Feed analysis

9

METHODS OF ANALYSIS TO CONTROLUNDESIRABLE SUBSTANCES IN FEED


Free and total Gossypol Photometry Acetic Acid glacial (Reag. Ph. Eur.) PA-ACS-ISO 131008

Aniline (Reag. Ph. Eur.) PA-ACS 131156

N,N-Dimethylformamide (UV-IR-HPLC-GPC) PAI-ACS 361785

n-Hexane 95% (UV-IR-HPLC) PAI-ACS 363242

2-Propanol (HPLC-gradient grade-UV-IR) PAI 221090

Zinc metal, powder PRS 141783


Constituentsof Animal origin

Light microscopy Acetic Acid glacial (Reag. Ph. Eur.) PA-ACS-ISO 131008

Acetone QP 211007

Copper(II) Sulphate 5-hydrate (Reag. Ph. Eur.)PA-ACS-ISO

131270

Ethanol 96% v/v (USP, BP, Ph. Eur.) PRS-CODEX 141085

Fehling's A Reagent VINIKIT 624568

Fehling's B Reagent VINIKIT 624569



Hydrogen Peroxide 3% w/v (10 vol.) stabilized VINIKIT

622772

Iodine resublimed pearls PA-ACS 131771

Lead(II) Acetate 3-hydrate (Reag. Ph. Eur.)PA-ACS-ISO

131466


Potassium Iodide PA 121542

Potassium Sodium Tartrate 4-hydrate PA-ACS-ISO 131729


Sodium Hypochlorite solution 10% w/v QP 211921

Tetrachloroethylene PRS 141455

Polymerase Chain Reaction (PCR)

DNA-ExitusPlus™ A7089


METHODS OF ANALYSIS FOR THE DETERMINATIONOF CONSTITUENTS OF ANIMAL ORIGIN FOR THE OFFICIAL CONTROL OF FEED

10

The following table displays the full program of reagents usedfor feed control pursuant to Regulation (EC) No 152/2009.

Panreac reagent recommended For the determination of Code Package

Acetanilide (Reag. USP) PA Crude Protein. 121005.1209 250 g

Acetic Acid glacial(Reag. Ph. Eur.) PA-ACS-ISO

Carbadox, Chlorine from chlorides, Constituents of Animal Origin, Free and Total Gossypol, Halofuginone, Lactose, Sugar, Starch, Tryptophan, Urea.

131008.1611 1000 ml

131008.1612 2.5 l

131008.1214 5 l

Acetone (Reag. Ph. Eur.) PA-ACS-ISO

Chlorine from Chlorides, Crude Fibre. 131007.1611 1000 ml

131007.1612 2.5 l

131007.1214 5 l

Acetone QP Constituents of Animal Origin. 211007.1211 1000 ml

211007.1214 5 l

Acetonitrile (HPLC-gradient grade) PAI-ACS

Diclazuril. 221881.1611 1000 ml

221881.1612 2.5 l

221881.1646 4 l

221881.0314 5 l

Acetonitrile (UV-IR-HPLC-isocratic) PAI-ACS

Amprolium, Carbadox, Robenidine, Halofuginone. 361881.1611 1000 ml

361881.1612 2.5 l

Ammonia 30% (as NH3) PA-ACS Total Phosphorus. 131130.1611 1000 ml

131130.1612 2.5 l

131130.1214 5 l

Ammonia FixativeSolution 1% RV

Crude Protein. 283334.1214 5 l


Methyl Benzoquate HPLC with UV detection Dichloromethane stabilized with ~20 ppm of amylene (UV-IR-HPLC-HPLC preparative-GPC) PAI-ACS

361254




Olaquindox HPLC with UV detection Methanol (UV-IR-HPLC-HPLC isocratic) PAI-ACS 361091

Methanol (Reag. Ph. Eur.) PA-ACS-ISO 131091


Amprolium HPLC with UV detection Acetonitrile (UV-IR-HPLC-isocratic) PAI-ACS 361881


Sodium di-Hydrogen Phosphate 1-hydrate (Reag. Ph. Eur.) PA-ACS

131965

Sodium Perchlorate 1-hydrate (Reag. USP) PA-ACS 134387


Carbadox HPLC with UV detection Acetic Acid glacial (Reag. Ph. Eur.) PA-ACS-ISO 131008



Sodium Acetate anhydrous (Reag. Ph. Eur.) PA-ACS

131633


METHODS OF ANALYSIS TO CONTROL ILLEGALPRESENCE OF NO LONGER AUTHORISED ADDITIVES IN FEED

Feed analysis

11


Ammonium Acetate (Reag. Ph. Eur.) PA-ACS

Diclazuril, Halofuginone. 131114.1210 500 g

131114.1211 1000 g

131114.0914 5 kg

Ammonium Iron(III) Sulphate 12-hydrate (Reag. Ph. Eur.) PA-ACS-ISO

Chlorine from Chlorides. 131365.1210 500 g

131365.1211 1000 g

Ammonium Molybdate 4-hydrate (Reag. Ph. Eur.) PA-ACS-ISO

Total Phosphorus. 131134.1208 100 g

131134.1209 250 g

131134.1211 1000 g

Ammonium Thiocyanate 0,1 mol/l (0,1N) (Reag. USP, Ph.Eur.) SV

Chlorine from chlorides. 181144.1211 1000 ml

Ammonium meta-Vanadate (Reag. USP, Ph. Eur.) PA-ACS

Total Phosphorus 132352.1209 250 g

132352.1211 1000 g

Aniline (Reag. Ph. Eur.) PA-ACS Free and Total Gossypol. 131156.1609 250 ml

Barium Hydroxide 8-hydrate (Reag. Ph. Eur.) PA-ACS-ISO

Tryptophan. 131188.1210 500 g

131188.1211 1000 g

Boric Acid PA-ACS-ISO Crude Protein, Volatile Nitrogenous Bases. 131015.1210 500 g

131015.1211 1000 g

131015.1214 5 kg

Boric Acid solution 3% RV Crude Protein. 282928.1211 1000 ml

Boric Acid solution 4% RV Crude Protein. 282222.1211 1000 ml

282222.1214 5 l

Bromocresol Green PA-ACS Crude Protein, Volatile Nitrogenous Bases. 131759.1603 1 g

131759.1604 5 g

131759.1606 25 g

Buffer Solution pH 4,00 ±0,02 (20°C) ST

Crude Protein. 272168.1209 250 ml

272168.1211 1000 ml

272168.1214 5 l

272168.1315 10 l

Buffer Solution pH 7,00 ±0,02 (20°C) ST


272170.1211 1000 ml

272170.1214 5 l

272170.1315 10 l

Calcium Carbonate precipitated PA

Carbonates, Total phosphorus. 121212.1210 500 g

121212.1211 1000 g

Carrez’s Reagent I RE Chlorine from Chlorides, Lactose, Starch, Sugar, Urea. 173355.1211 1000 ml

173355.1214 5 l

Carrez’s Reagent II RE Chlorine from Chlorides, Lactose, Starch, Sugar, Urea. 173356.1211 1000 ml

173356.1214 5 l

Celite Hyflo Super Cel ® RE Crude Fibre, Crude Oils and Fats. 175772.1210 500 g

Charcoal Activated powder PA Chlorine from Chlorides, Urea. 121237.1609 250 g

121237.1610 500 g

Citric Acid 1-hydratePA-ACS-ISO

Lactose, Sugar. 131018.1210 500 g

131018.1211 1000 g

131018.1214 5 kg

Copper standard solution Cu=1,000±0,002 g/l AA

Trace Elements Iron, Copper, Manganese and Zinc. 313178.1209 250 ml

313178.1210 500 ml

Copper(II) Oxide PRS Crude Protein. 141269.1210 500 g

12


Copper(II) Sulphate 5-hydrate (Reag. Ph. Eur.) PA-ACS-ISO

Constituents of Animal Origin, Crude Protein, Lactose, Sugar. 131270.1210 500 g

131270.1211 1000 g

131270.1214 5 kg

Detergent Acid Solution RE Crude Fibre. 175055.1214 5 l

Detergent Neutral Solution RE Crude Fibre. 175054.1214 5 l

2,6-Di-tert-Butyl-4-Methylphenol (RFE, BP, Ph. Eur.) PRS-CODEX

Vitamine A, Vitamine E. 142825.1211 1000 g

142825.0914 5 kg

Dichloromethane stabilized with ~20 ppm of amylene (UV-IR-HPLC-HPLC preparative-GPC) PAI-ACS

Methyl Benzoquate. 361254.1611 1000 ml

361254.1612 2.5 l

Diethyl Ether stabilized with ~6 ppm of BHT (Reag. Ph. Eur.) PA-ACS-ISO

Chlorine from Chlorides. 132770.0311 1000 ml

132770.0314 5 l

4-(Dimethylamino) Benzaldehyde PA

Urea. 121293.1608 100 g

N,N-Dimethylformamide (UV-IR-HPLC-GPC) PAI-ACS

Diclazuril, Free and Total Gossypol. 361785.1611 1000 ml

361785.1612 2.5 l

DNA-ExitusPlus™ Constituents of Animal Origin. A7089

Ethanol absolute PA-ACS-ISO Lactose, Starch, Sugar. 131086.1611 1000 ml

131086.1612 2.5 l

131086.1214 5 l

131086.1315 10 l

Ethanol 96% v/v PA Chlorine from Chlorides, Crude Protein, Urea, Vitamine A, Vitamine E, Volatile Nitrogenous Bases.

121085.1211 1000 ml

121085.1212 2.5 l

121085.1214 5 l

121085.1315 10 l

Ethanol 96% v/v (USP, BP, Ph. Eur.) PRS-CODEX

Constituents of Animal Origin. 141085.1211 1000 ml

141085.1214 5 l

141085.1315 10 l

Ethanolamine PA-ACS Tryptophan. 131924.1611 1000 ml

Ethyl Acetate (UV-IR-HPLC-HPLC preparative) PAI-ACS

Halofuginone. 361318.1611 1000 ml

361318.1612 2.5 l

Ethylenediaminetetraacetic Acid Disodium Salt 2-hydrate (Reag. Ph. Eur.) PA-ACS

Halofuginone. 131669.1209 250 g

131669.1210 500 g

131669.1211 1000 g

131669.0914 5 kg

Fehling’s A Reagent VINIKIT Constituents of Animal Origin. 624568.1209 250 ml

Fehling’s B Reagent VINIKIT Constituents of Animal Origin. 624569.1209 250 ml

Formic Acid 98% PA-ACS Amino Acids (Except Tryptophan). 131030.1611 1000 ml

131030.1612 2.5 l

Glycerol PA-ACS-ISO Constituents of Animal Origin, Vitamine A, Vitamine E. 131339.1211 1000 ml

131339.1212 2.5 l

131339.1214 5 l

n-Hexane 95% (UV-IR-HPLC) PAI-ACS

Free and Total Gossypol. 363242.1611 1000 ml

363242.1612 2.5 l

Hydrochloric Acid 37%PA-ACS-ISO

Amino Acids (Except Tryptophan), Diclazuril, Lactose,Lasalocid Sodium, Methyl Benzoquate, Robenidine, Sugar,Trace Elements Iron, Copper, Manganese and Zinc, Tryptophan, Urea.

131020.1611 1000 ml

131020.1612 2.5 l

131020.1214 5 l

Feed analysis

13


Hydrochloric Acid 32% PA-ISO Halofuginone 132176.1611 1000 ml

132176.1612 2.5 l

132176.1214 5 l

Hydrochloric Acid 25% PA-ISO Carbonates, Starch, Total Phosphorus. 133378.1611 1000 ml

133378.1612 2.5 l

133378.1214 5 l

Hydrochloric Acid 0,1 mol/l (0,1N) SV

Constituents of Animal Origin, Crude Protein, Halofuginone, Lactose, Sugar, Tryptophan.

181023.1211 1000 ml

181023.1212 2.5 l

181023.1214 5 l

181023.1315 10 l

Hydrochloric Acid 0,5 mol/l (0,5N) SV

Crude Fibre, Trace Elements Iron, Copper, Manganese and Zinc. 181022.1211 1000 ml

181022.1212 2.5 l

181022.1315 10 l

Hydrochloric Acid 1 mol/l (1N) SV

Constituents of Animal Origin, Tryptophan. 181021.1211 1000 ml

181021.1212 2.5 l

181021.1214 5 l

181021.1315 10 l

Hydrochloric Acid 3 mol/l (3N) SV

Crude Oils and Fats, Hydrochloric Acid Insoluble Ash. 182057.1211 1000 ml

182057.1315 10 l

Hydrofluoric Acid 48%PA-ACS-ISO


131028.1212 2.5 l

Hydrogen Peroxide 33% w/v (110 vol.) PA-ACS-ISO

Amino Acids (Except Tryptophan), Trace Elements Iron, Copper, Manganese and Zinc.

131077.1210 500 ml

131077.1211 1000 ml

131077.1214 5 l

Hydrogen Peroxide 3% w/v(10 vol.) stabilized VINIKIT


622772.1211 1000 ml

Indicator 4,8, Mixed (Methyl Red-Bromocresol Green) RV

Crude Protein, Volatile Nitrogenous Bases. 283303.1609 250 ml

Iodine resublimed pearlsPA-ACS

Constituents of Animal Origin. 131771.1608 100 g

131771.1609 250 g

131771.1610 500 g

131771.1611 1000 g

Iron standard solution Fe=1,000±0,002 g/l AA


313182.1210 500 ml

Kjeldahl Catalyst (Cu) (6,25% in CuSO4.5H2O) tablets RE

Crude Protein. 174428.1246 4 kg

Lanthanum(III) Chloride 7-hydrate PA

Trace Elements Iron, Copper, Manganese and Zinc. 122848.1208 100 g

122848.1209 250 g

Lanthanum(III) Oxide(Reag. Ph. Eur.) PA

Trace Elements Iron, Copper, Manganese and Zinc. 122705.1607 50 g

122705.1609 250 g

Lead(II) Acetate 3-hydrate (Reag. Ph. Eur.) PA-ACS-ISO


131466.1214 5 kg

Luff-Schoorl’s Reagent RE Lactose, Sugar. 172174.1211 1000 ml

Magnesium Oxide light (RFE, BP, Ph. Eur.) PRS-CODEX

Volatile Nitrogenous Bases. 141276.1211 1000 g

Manganese standard solution Mn=1,000±0,002 g/l AA


313185.1210 500 ml

14


Methanol (HPLC-gradient grade) PAI-ACS

Diclazuril. 221091.1611 1000 ml

221091.1646 4 l

221091.0314 5 l

Methanol (UV-IR-HPLC-HPLC isocratic) PAI-ACS

Amprolium, Carbadox, Halofuginone, Lasalocid Sodium, Methyl Benzoquate, Olaquindox, Robenidine, Tryptophan, Vitamine A, Vitamine E.

361091.1611 1000 ml

361091.1612 2.5 l

361091.1646 4 l

361091.0314 5 l

Methanol (Reag. Ph. Eur.)PA-ACS-ISO

Olaquindox. 131091.1611 1000 ml

131091.1612 2.5 l

131091.1214 5 l

3-Methyl-1-Butanol(Reag. Ph. Eur.) PA-ACS

Lactose, Sugar. 131079.1611 1000 ml

Methyl Orange solution 0,1% RV Lactose, Sugar. 281432.1208 100 ml

281432.1209 250 ml

Methyl Red PA-ACS Crude Protein, Volatile Nitrogenous Bases. 131617.1605 10 g

131617.1608 100 g

Methyl Red solution 0,1% RV Carbonates, Crude Protein, Starch, Volatile Nitrogenous Bases. 281618.1208 100 ml

Ninhydrin PA-ACS Amino Acids (Except Tryptophan). 132362.1605 10 g

132362.1608 100 g

132362.1611 1000 g

Nitric Acid 69% PA-ACS-ISO Total Phosphorus. 131037.1611 1000 ml

131037.1612 2.5 l

131037.1214 5 l

Nitric Acid 65% PA-ISO Chlorine from Chlorides, Total Phosphorus. 133255.1611 1000 ml

133255.1612 2.5 l

1-Octanol (Reag. USP) PA-ACS Amino Acids (Except Tryptophan), Crude Fibre. 133386.1610 500 ml

Petroleum Ether 40-60°CPA-ACS-ISO

Amino Acids (Except Tryptophan), Crude Fibre, Crude Oils and Fats, Tryptophan, Vitamine A, Vitamine E.

131315.1611 1000 ml

131315.1612 2.5 l

131315.0314 5 l

Phenol crystallized (detached crystals) PA-ACS

Amino Acids (Except Tryptophan). 134852.1209 250 g

134852.1211 1000 g

Phenolphthalein solution 2% RE Vitamine A, Vitamine E. 172866.1210 500 ml

ortho-Phosphoric Acid 85% PA-ACS-ISO

Lasalocid Sodium, Tryptophan. 131032.1211 1000 ml

131032.1212 2.5 l

131032.1214 5 l

di-Phosphorus penta-Oxide PRS

Amino Acids (Except Tryptophan), Tryptophan. 141154.1210 500 g

141154.1211 1000 g

Potassium Carbonate PA Volatile Nitrogenous Bases. 121490.1211 1000 g

Potassium Hexacyanoferrate(II) 3-hydrate (Reag. Ph. Eur.)PA-ACS-ISO

Chlorine from chlorides, Lactose, Starch, Sugar, Urea. 131505.1210 500 g

131505.1211 1000 g

Potassium di-Hydrogen Phosphate PA

Lasalocid sodium, Robenidine, Total Phosphorus. 121509.1210 500 g

121509.1211 1000 g

Potassium Hydroxide 85% pellets PA

Constituents of Animal Origin, Vitamine A, Vitamine E. 121515.1210 500 g

121515.1211 1000 g

121515.1214 5 kg

Feed analysis

15


Potassium Hydroxide 0,23 mol/l (0,23N) SV

Crude Fibre. 183354.1214 5 l

183354.1315 10 l

Potassium Iodide PA Constituents of Animal Origin, Lactose, Sugar. 121542.1209 250 g

121542.1210 500 g

121542.1211 1000 g

121542.1214 5 kg

Potassium Iodide solution 30% w/v VINIKIT


Potassium Sodium Tartrate 4-hydrate PA-ACS-ISO


131729.1211 1000 g

131729.1214 5 kg

Potassium Sulphate(Reag. Ph. Eur.) PA-ACS-ISO

Crude Protein. 131532.1210 500 g

131532.1211 1000 g

131532.1214 5 kg

2-Propanol (HPLC-gradient grade-UV-IR) PAI

Free and total Gossypol, Vitamine A. 221090.1611 1000 ml

221090.1612 2.5 l

Pumice Stone granules QP Crude Protein, Lactose, Sugar. 211835.1209 250 g

211835.1210 500 g

211835.0914 5 kg

Saccharose PA-ACS Crude Protein. 131621.1210 500 g

131621.1211 1000 g

131621.0914 5 kg

Silicone antifoaming liquid (AQ) QP

Volatile Nitrogenous Bases. 216241.1210 500 ml

Silver Nitrate PA-ACS-ISO Halofuginone. 131459.1606 25 g

131459.1608 100 g

131459.1609 250 g

131459.1611 1000 g

Silver Nitrate 0,1 mol/l (0,1N) SV Chlorine from Chlorides, Halofuginone. 181464.1610 500 ml

181464.1211 1000 ml

181464.1315 10 l

Sodium Acetate anhydrous (Reag. Ph. Eur.) PA-ACS

Carbadox. 131633.1210 500 g

131633.1211 1000 g

Sodium L(+)-Ascorbate (USP) PRS-CODEX

Halofuginone, Vitamine A, Vitamine E. 143865.1211 1000 g

Sodium Carbonate anhydrous (Reag. Ph. Eur.) PA-ACS

Halofuginone, Lactose, Sugar. 131648.1210 500 g

131648.1211 1000 g

131648.0914 5 kg

Sodium Chloride PA-ACS-ISO Amino Acids (Except Tryptophan). 131659.1210 500 g

131659.1211 1000 g

131659.1214 5 kg

Sodium Chloride PA Amino Acids (Except Tryptophan), Halofuginone. 121659.1210 500 g

121659.1211 1000 g

121659.1214 5 kg

tri-Sodium Citrate 2-hydrate PA-ACS


131655.1211 1000 g

131655.1214 5 kg

16


Sodium Disulphite PA-ACS Amino Acids (Except Tryptophan). 131698.1210 500 g

131698.1211 1000 g

131698.1214 5 kg

di-Sodium Hydrogen Phosphate anhydrous (Reag. Ph. Eur.) PA-ACS

Robenidine. 131679.1210 500 g

131679.1211 1000 g

131679.0914 5 kg

di-Sodium Hydrogen Phosphate 2-hydrate PA

Robenidine. 122507.1210 500 g

122507.1211 1000 g

di-Sodium Hydrogen Phosphate 12-hydrate PA-ISO

Robenidine. 131678.1210 500 g

131678.1211 1000 g

131678.1214 5 kg

Sodium di-Hydrogen Phosphate 1-hydrate (Reag. Ph. Eur.) PA-ACS

Amprolium. 131965.1210 500 g

131965.1211 1000 g

Sodium Hydroxide pelletsPA-ACS-ISO

Amino Acids (Except Tryptophan), Constituents of Animal Origin, Tryptophan.

131687.1210 500 g

131687.1211 1000 g

131687.1214 5 kg

Sodium Hydroxide solution 40% w/v PA

Crude Protein. 121593.1214 5 l

Sodium Hydroxide solution 40% w/w RE


171220.1214 5 l

171220.1315 10 l

171220.0716 25 l

Sodium Hydroxide 0,1 mol/l (0,1N) SV

Crude Protein, Lactose, Starch, Sugar, Volatile Nitrogenous Bases. 181693.1211 1000 ml

181693.1212 2.5 l

181693.1214 5 l

181693.1315 10 l




Crude Fibre. 183337.1214 5 l

Sodium Hydroxide 1 mol/l (1N) SV

Amino Acids (Except Tryptophan), Tryptophan. 181691.1211 1000 ml

181691.1212 2.5 l

181691.1214 5 l

181691.1315 10 l

Sodium Hypochlorite solution 10% w/v QP


Sodium Perchlorate 1-hydrate (Reag. USP) PA-ACS

Amprolium. 134387.1208 100 g

134387.1210 500 g

Sodium Sulphate anhydrous (Reag. USP) PA-ACS-ISO

Crude Oils and Fats. 131716.1210 500 g

131716.1211 1000 g

131716.1214 5 kg

Sodium Sulphide x-hydrate QP Vitamine A, Vitamine E. 211682.1610 500 g

Sodium Thiosulphate 0,1 mol/l (0,1N) (Reag. USP) SV


181723.1212 2.5 l

181723.1315 10 l

Phosphorus standard solution P=1,000±0,002 g/l IC

Total Phosphorus. 786922.1210 500 ml

Feed analysis

17


Starch from Potato soluble (Reag. USP, Ph. Eur.) PA

Lactose, Sugar. 121096.1210 500 g

121096.1211 1000 g

Starch solution 1% RV Lactose, Sugar. 283146.1209 250 ml

283146.1211 1000 ml

5-Sulphosalicylic Acid 2-hydrate (Reag. Ph. Eur.) PA-ACS


132838.1211 1000 g

Sulphuric Acid 98% RE Crude Protein. 173163.1611 1000 ml

173163.1612 2.5 l

Sulphuric Acid 96% PA-ISO Lactose, Sugar, Total Phosphorus, Trace Elements Iron, Copper, Manganese and Zinc.

131058.1611 1000 ml

131058.1612 2.5 l

131058.1214 5 l

Sulphuric Acid 0,01 mol/l (0,02N) SV

Volatile Nitrogenous Bases. 182102.1211 1000 ml


Carbonates, Crude Protein, Volatile Nitrogenous Bases. 181061.1211 1000 ml

181061.1214 5 l

181061.1315 10 l




Crude Fibre. 183335.1214 5 l

183335.1315 10 l

Sulphuric Acid 0,13 mol/l (0,26N) RE

Crude Fibre. 176191.1211 1000 ml

176191.1214 5 l



181060.1212 2.5 l

181060.1315 10 l

Tetrabutylammonium Hydrogen Sulphate (HPLC) PAI

Diclazuril. 363622.1605 10 g

363622.1606 25 g

363622.1607 50 g

363622.1610 500 g

Tetrachloroethylene PRS Constituents of Animal Origin. 141455.1611 1000 ml

141455.1612 2.5 l

141455.1714 5 l

Trichloroacetic Acid(Reag. Ph. Eur.) PA-ACS

Hydrochloric Acid Insoluble Ash. 131067.1608 100 g

131067.1609 250 g

131067.1611 1000 g

131067.1214 5 kg

Trichloroacetic Acid solution 20% w/v DC

Hydrochloric Acid Insoluble Ash, Volatile Nitrogenous Bases. 252373.1611 1000 ml

252373.1611 1000 ml

1,1,1-Trichloro-2-Methyl-2-Propanol 1/2-hydrate (RFE, BP, Ph. Eur.) PRS-CODEX

Tryptophan. 145300.1211 1000 g

Water (UV-HPLC) PAI-ACS Amprolium, Carbadox, Diclazuril, Halofuginone, Lasalocid Sodium, Methyl Benzoquate, Olaquindox, Robenidine.

361074.1611 1000 ml

361074.1612 2.5 l

Zinc standard solution Zn=1,000±0,002 g/l AA


313193.1210 500 ml

Zinc metal, powder PRS Free and Total Gossypol. 141783.1610 500 g

141783.1611 1000 g

Zinc Acetate 2-hydrate (Reag. USP, Ph. Eur.) PA-ACS

Chlorine from chlorides, Lactose, Starch, Sugar, Urea. 131775.1210 500 g

131775.1211 1000 g

18

Panreac offers the following CULTIMEDculture media for microbiological control.

Microorganism Stage Recommended culture medium CULTIMED As standard Code Package

Coliforms

E. coli Selective detection

Chromogenic E. coli Agar(Dehydrated Culture Media)

416109.12133416109.12135

525 g 105 g

E.coli, Chromogenic Agar (Prepared Plate (Ø 90 mm))

456109.0952 10 plates

Selective detection / Enumeration

TBX Agar (ISO 16649-2:2000)(Dehydrated Culture Media)

ISO 16649-2

416220.1210 500 g

TBX Agar (ISO 16649-2:2000)(Prepared Plate (Ø 90 mm))

456220.0952 10 plates

Enterobacteriaceae Culture / Enumeration

Violet Red Bile Glucose Agar (VRBG) (Ph. Eur. ) (ISO21528) (Dehydrated Culture Media)

413745.1210 500 g

Violet Red Bile Glucose Agar (VRBG) (Ph. Eur.) (Contact Plate)

433745.0922 30 plates

Violet Red Bile Glucose Agar (VRBG) (Ph. Eur.) (Prepared Plate (Ø 90 mm))

453745.0922 20 plates

Isolation / Differentiation

Hektoen Enteric Agar (Dehydrated Culture Media) 413768.1210 500 g

Isolation / Identification

MacConkey Agar (Ph. Eur.)(Dehydrated Culture Media)

Ph.Eur. 413779.1210 500 g

Differentiation MR-VP Medium (Dehydrated Culture Media) 413786.1210 500 g

Pathogens

Salmonella Diluent agent Buffered Peptone Water (ISO 6579:2002) (Dehydrated Culture Media)

ISO 6579

413795.1210 500 g

Buffered Peptone Water (ISO 6579:2002) (Prepared Bottles)

493795.0922 10x100 ml

Buffered Peptone Water (ISO 6579:2002) (3 L bags) 493795.0981 3 x 3 l

Selective enrichment

Rappaport-Vassiliadis (RVS) Broth (ISO 6579:2002) (Dehydrated Culture Media)

ISO 6579 414959.1210 500 g


Tetrathionate Broth Base acc. to Muller-Kauffmann (Dehydrated Culture Media)

414961.1210 500 g


Selenite Cystine Broth (Dehydrated Culture Media) 413809.1210 500 g

Selenite Cystine Broth (Prepared Tubes) 463809.0922 20 tubes

Differentiation Lysine Iron Agar (Dehydrated Culture Media) 413770.1210 500 g

Culture Nutrient Agar (Dehydrated Culture Media) 413792.1210 500 g

Isolation Chromogenic Salmonella Agar (Dehydrated Culture Media)

416110.12134 575 g

Salmonella Chromogenic Media (Prepared Plate (Ø 90 mm))

456110.0952 10 plates

Selective isolation Salmonella Shigella Agar(Dehydrated Culture Media)

413805.1210 500 g

Selective isolation XLD Agar (ISO 6579:2002)(Dehydrated Culture Media)

ISO 6579

416270.1210 500 g

XLD, Agar (ISO 6579:2002)(Prepared Plate (Ø 90 mm))

456270.0922 20 plates

Selective isolation Brilliant Green Agar (Dehydrated Culture Media) Ph. Eur. 413823.1210 500 g

Feed analysis

19

Microorganism Stage Recommended culture medium CULTIMED As standard Code Package

Listeria monocytogenes

Enrichment Fraser Listeria Broth Base (ISO 11290-1:1996) (Dehydrated Culture Media)

ISO 11290-1

416112.1210 500 g

Fraser 1/2 Listeria Selective Enrichment Supplement (Additive)

416114.02131 2 x 5 vials

Fraser Listeria Selective Enrichment Supplement (Additive)

416113.02131 2 x 5 vials

Selectivemedium / Differentiation

Oxford Listeria Selective Supplement (Additive) 416115.02132 10 vials

PALCAM Listeria Agar Base (Dehydrated Culture Media)

415380.1210 500 g

Selective detection / Enumeration

Listeria Chromogenic Agar (ISO 11290-1:2004) (Dehydrated Culture Media)

416891.1210 500 g

Listeria Chromogenic Agar (ISO 11290-1:2004) (Prepared Plate (Ø 90 mm))

456891.0952 10 plates

Other microorganisms

Staphylococcus aureus

Selective isolation Baird-Parker Agar Base(Dehydrated Culture Media)

ISO 6888-1 413744.1210 500 g

413744.0914 5 kg

Selective isolation RPF Supplement (ISO-FDIS 6888-2) (Additive) ISO 6888-2 416272.02132 10 viales

Selective isolation Egg Yolk Tellurite Emulsion (Additive) ISO 6888-1 414723.1607 50 ml

414723.1608 100 ml

Isolation Mannitol Salt Agar (Ph. Eur.) (Dehydrated Culture Media)

Ph. Eur. 413783.1210 500 g

Confirmation Brain Heart Infusion (BHI) (Dehydrated Culture Media)

ISO 6888-1 413777.1210 500 g

Clostridium perfringens

Isolation SPS Agar (Selective Agar according to Angelotti)(Dehydrated Culture Media)

414125.1210 500 g

Detection / Enumeration

TSC Agar Base (ISO 7937) (Dehydrated CultureMedia)

ISO 7937

415576.1210 500 g

TSC Agar Base (ISO 7937) (Prepared Bottles) 495576.0922 10x100 ml

Selective isolation TSN Agar (Dehydrated Culture Media) 413833.1210 500 g

Enterobacter sakazakii

Selective isolation Enterobacter Sakazakii Chromogenic Agar(ISO 22964) (Dehydrated Culture Media)

ISO 22964 416960.12163 520 g

Enterobacter Sakazakii Chromogenic Agar (ISO 22964) (Prepared Plate (Ø 90 mm))

456960.0952 10 plates

Vibrio cholerae Isolation TCBS Cholera Medium (Dehydrated Culture Media)

413817.1210 500 g

413817.0914 5 kg

Yeasts and molds

Culture Malt Extract Agar (Dehydrated Culture Media) 413781.1210 500 g

Identification / Culture / Enumeration

Potato Glucose Agar (Ph. Eur.) (Dehydrated Culture Media)

Ph.Eur. 413758.1210 500 g

Culture / Selective isolation

Rose Bengal Chloramphenicol Agar (Dehydrated Culture Media)

414855.1210 500 g

Rose Bengal Chloramphenicol Agar (Contact Plate)

434855.0922 30 plates

Panreac Química S.L.U.C/ Garraf, 2 - Polígono Pla de la BrugueraE-08211 Castellar del Vallès(Barcelona) EspañaTel. (+34) 937 489 400Fax (+34) 937 489 401e-mail: [email protected]

Panreac Chimie S.A.R.L.129, rue ServientTour Credit Lyonnais69326 Lyon Cedex 03FranceTel. (+34) 902 438 439Fax (+34) 937 489 495e-mail: [email protected]

Nova Chimica S.r.l.Via G. Galilei, 4720092 Cinisello Balsamo (MI)ItalyTel. +39 02 66045392Fax +39 02 66045394e-mail: [email protected]

www.panreac.com

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