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@<QKT+9CF细胞 6.5@0 基因启动子甲基化和
组蛋白乙酰化状态与多药耐药的关系
黄程辉6
! 曹培国6
! 谢兆霞"
!中南大学!6/湘雅三医院肿瘤科"长沙 &6##6% #"/湘雅医院血液科"长沙 &6##T4 $
!摘要"!目的#分析 @<QKT +9CF及 @<QKT 细胞 6.5@0 基因启动子甲基化和组蛋白乙酰化状态!初
步探讨乳腺癌多药耐药的表观遗传机制" 方法#用甲基化敏感 R<S技术检测两个细胞系 6.5@0 基因启
动子甲基化状态" 实时定量 R<S技术检测 A:9甲基转移酶 #A:913)(.E)FB20H3FB030!2A-<?$ 1S:9及组
蛋白去乙酰化酶# (?0)G23C3BD3).EB030!>2E"?$ 1S:9的表达" 光密度值法检测组蛋白 7% 和 7& 乙酰化水
平" 结果#@<QKT 细胞 6.5@0 基因启动子呈现高甲基化!@<QKT +9CF细胞 6.5@0 基因启动子呈现低甲基
$]%
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化" 与 @<QKT 细胞比较!@<QKT +9CF细胞 2A-<0 !2A-<B4 及 2A-<BC1S:9表达显著下降 #D\#/#' $ "
@<QKT +9CF细胞组蛋白 7% 和 7& 乙酰化水平较 @<QKT 细胞明显升高 #D\#/#6 $ " 与 @<QKT 细胞比
较!@<QKT +9CF细胞 >2E"0 !>2E"F !>2E"G 及 &HI<0 1S:9的表达显著下降#D\#/#6 $ " 结论#6.5@0 基
因启动子低甲基化%组蛋白 7% 和 7& 高乙酰化%2A-<?1S:9及 >2E"?1S:9低表达可能是介导 @<QK
T +9CF细胞 @AS形成的重要表观遗传学因素"
!关键词"! 乳腺癌& !多药耐药& !基因&!甲基化&!组蛋白&!乙酰化
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