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program on anthropometric measures and metabolic blood parameters in69 postmenopausal overweight women (59.2±5.0 y; 32.8±3.6 kg/m2).Subjects replaced 2 daily meals for the first 6 weeks, followed by thereplacement of 1 meal for the next 18 weeks. Dietary fat intake should notexceed 60 g/d. Subjects increased physical activity by attending two timesweekly a 60 minute endurance program. All participants attended more than80% of the meetings. Before and after intervention body composition wasmeasured by air displacement plethysmography (Bod Pod®), plasma levelsof Apo-B, HDL-C, LDL-C, triglycerides, glucose, insulin, leptin, cortisolwere determined by routine methods. – All tested variables improvedsignificantly: BW -6.4kg, BMI -2.4kg/m2, FM -6.0kg, Apo-B -8mg/l,HDL-C +4.1mg/l, LDL-C -10mg/l, triglycerides -33mg/l, glucose -3mg/l,insulin -2.2µU/ml, leptin -19ng/ml, cortisol -5µg/dl. - The results clearlydemonstrate that the combination of meal replacement and guided physicalexercise lead to a significant and safe improvement of the atherogenicrisk profile even in postmenopausal women. After the intervention theprevalence of the metabolic syndrome dropped from 76 to 18% in thewomen examined.

160 FAVORABLE METABOLIC PROPERTIES OF ASOY-HONEY-YOGHURT PRODUCT FOR MEALREPLACEMENT IN OVERWEIGHT SUBJECTS WITHATHEROGENIC RISK

A. Berg1, D. Koenig1, P. Deibert1 , U. Landmann1, I. Frey1, B. Kloock1,A. Gollhofer2 . 1Medizinische Universitaetsklinik Freiburg, AbteilungPraeventive und Rehabilitative Sportmedizin, Freiburg i. Br., Germany;2Albert-Ludwigs-Universitaet Freiburg, Institut fuer Sport undSportwissenschaft, Freiburg i. Br., Germany

The supplement tested is well established as a meal-replacement forweight-management and in the therapy of the metabolic syndrome. Asa soy-yoghurt-honey preparation (Almased®) it shows significant benefitscompared with conventional low-caloric diets. Nevertheless, the mechanismof this metabolic benefit is not clearly understood. – Therefore, 10 clinicallyhealthy adults were tested for their glucose as well as hormonal reactionduring the first 2 hours after intake of different meals in the fasting state(8.00-9.00a.m.). A carbohydrate-rich breakfast (CB), the tested product(TP) and a glucose drink (GD) were given to WHO/FAO criteria for GItesting. Kinetics (0, 15, 30, 45, 60, 90, 120 min after intake) was analyzedfor plasma insulin, cortisol, leptin, ghrelin, and serotonin and blood glucose.– The calculated kinetics clearly demonstrate that the glucose and insulinresponse after TD were significantly lower than after CB or GD andresponsible for a very low GI of 27. Compared with CB and GD, plasmaghrelin decreased significantly more after TP ingestion (-27%/-18%/-35%)and remained at this level even after 2 hours of TP intake. Cortisol, leptinand serotonin showed a similar response after CB, TP and GD intake. –The postprandial insulin as well as ghrelin response after TD comparedwith CB and GD suggests a favourable influence of TD in programmingthe appetite regulatory system. This may explain the specific benefit of thetested soy-honey-yoghurt product for weight control and insulin resistanceafter regularly intake in subjects with increased metabolic risk.

161 DIFFERENT MARKERS OF CENTRAL OBESITY ASPREDICTORS OF INSULIN RESISTANCE ANDINFLAMMATORY STATUS IN MIDDLE-AGED WOMEN

S. Zecova1, M. Lejskova2, Z. Valenta3 , P. Stavek4, R. Poledne4, J. Pitha4.1Department of internal medicine I, Thomayer’s teachinghospital,Prague,Czech Republic; 2Institute for Postgraduate MedicalEducation, Prague,Czech Republic; 3Institute of Computer Science AS CRPrague, Czech Republic; 4Institute of Clinical and ExperimentalMedicine,Prague,Czech Republic

Background: In women approaching menopause central obesity could beone of the most important risk factors. Its detrimental effects on the vesselwall could be mediated through insulin resistance and/or inflammatorystatus. Therefore, detection of reliable marker of central obesity for clinicalpractice is of importance. We evaluated the association of three differentmarkers of central obesity with parameters of insulin resistance andinflammatory status

Methods: Representative population sample of women aged 45-54years (n=639). We tested statistical significance of the association betweeninsulin resistance expressed as homeostasis model for insulin resistance(HOMA-IR: fasting insulin in mU/L multiplied by fasting glycaemia in

mmol/L and divided by 22.5) and between C-reactive protein measuredby highly sensitive method with following parameters of central obesity:waist circumference, waist/hip ratio and central subcutaneous fat (aboveumbilicus) measured by already established method using ultrasound.

Results: The strongest association with insulin resistance was foundwith the waist/hip ratio (when standardized for age, body mass index,plasma triglycerides and smoking). The strongest association with C-reactive protein was found with subcutaneous fat measured by ultrasound(when standardized for age, body mass index, plasma triglycerides andsmoking).

Conclusion: Among different parameters of central obesity, waist/hipratio and central subcutaneous fat measured by ultrasound were thestrongest independent predictors for insulin resistance and inflammatorystatus, respectively.

This work was supported by grant Ministry of Education Youth andSports of the Czech Republic No. 1M0510

162 ASSOCIATION OF APO E GENE POLYMORPHISM ANDOBESITY IN IRANIAN POPULATION:TEHRAN LIPIDAND GLUCOSE STUDY

M. Hedayati, M. Daneshpour, F. Azizi. Obesity Research Center, ResearchInstitute for Endocrine Sciences, Tehran, Iran

Introduction: The Apo E is one of the genes, which play a role in theregulation of lipid metabolism, especially in obesity. In other studies acommon Apo E polymorphism has been shown to be associated withobesity, and in the present study our aim is to determine this association inan Iranian population.

Materials & methods: Subjects were randomly selected from the TehranLipid and Glucose Study and classified into 3 in three groups according totheir body mass index: BMI<25, 25≥BMI<30, BMI≥30 and finally 429(150 in men and 179 in women). We measured FBS, HDL-C, triglycerideand cholesterol levels and evaluated body mass index and blood pressure forall individuals. A segment of the mentioned gene with PCR was amplifiedand the polymorphism with RFLP (HhaI) revealed.

Results: The allele frequency of Apo E polymorphism was in the HardyWeinberg equilibrium and the allele frequency was ε2 (0.065), ε3 (0.851)and ε4 (0.083); there was no relation between BMI and the frequency ofthis allele.

Conclusion: These results show that there is no relation between Apo Epolymorphism and BMI in this study.

163 ASSOCIATION OF HELICOBACTER PYLORISEROPOSITIVITY WITH CORONARY ARTERY DISEASE

A.E. Esmaeili Nadimi1, A.J. Jafarzadeh2 . 1Cardiology, Medical Faculty,Aliebneabitaleb, Rafsanjan, Kerman, Iran; 2Immunology, Medical Faculty,Rafsanjan, Kerman, Iran

Background and aims: The role of inflammation in the pathogenesis ofcoronary artery disease (CAD) has been increasingly discussed, but stillremains unclear. Several epidemiological and clinical studies suggested thathelicobacter pylori (HP) infection has been associated with an increased riskof developing CAD. We investigated HP infection and specifically whetherthe risk is confined to infection with the more virulent strains bearing thecytotoxin associated gene-A (cagA) antigen is related to coronary arterydisease.

Method: In this study three equal groups 60 individuals were investi-gated (Acute myocardial infarction (AMI), unstable angina (UA) class IIIBand control). Helicobacter pylora -specific IgG antibody and Serologicalstatus for cagA were measured. Antibody titer and the mean of Antibody inthree groups were compared and data analysis with chi square and ANOVAwas done.

Results: 86.7% of patients with AMI, 91.7% of UA, and 58.3% ofcontrol group were seropositive and this value was significantly higher inpatients groups.

The mean of antibody titer was 33.20, 57.96 and 25.72 u/ml in AMI, UAand control groups respectively. This measure was significantly different inUA group. Anti –cagA was positive in 55.8, 65.7 and 65.7 in AMI, UAand control groups respectively and the difference of this value was notsignificant between groups.

Conclusions: Our study showed that HP seropositivity and mean ofantibody levels in patients with CAD were significantly higher than controlgroup and anti-cagA was not different between the groups, this means that

77th Congress of the European Atherosclerosis Society, April 26–29, 2008, Istanbul, Turkey