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Fast Protein Liquid

Chromatography (FPLC)

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Column components

1. Packing

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Gel degassing.

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Stopping the outlet of the column.

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Filling liquid in the bottomof the column

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Pouring gell slurry to the column.

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Settling of gels.

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Adjusting the adaptor

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Fixed adaptor

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Conneting a lineto the adaptor

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Adaptor touching the upper layer of gels.

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Gel level lowers due tothe solution flowinto the column.

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Stopping the outletline

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Connetion of pumpline to the adaptor

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Blue dextran solution flows in the column.

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2. Valve control

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Injection of sample

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FPLC controller.

3. Remote controller

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Pump front panel.

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Pump flow ratecalibration

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Manual mode of gradient setting

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Valve number setting

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Flow rate setting

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Multimeter for digital data aquisition

4. Multimeter

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UV detector

5. Detector

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Software UV signaldata aquisition

6. Software(PROTEK 506)

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Multimeter settingfor sending data to notebook computer

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Sampling time setup

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File name.

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Save data file In a folder

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Starting data acquisitionEnd ing data collection