Fast and Precise!

Introducing the NucleoCounter®NC-250™How healthy are your cells? It’s VITAL to know!

New innovative Cell Counter!

Fast and Precise!

Precise and objectiveNo manual adjustments, just press RUN

High speed cell count8 samples in less than 3 minutes!

Counting & Analytical AssaysCell health determination via fast apoptosis assay

Low running costsReduce your running costs significantly

Calibration and maintenance freeInstantly ready

NC-250™ Highlights

Fast and Precise!

One-Step Viability and Cell Count Load and press Run

Two-Step Viability and Cell Count Micro-Carriers – extremely aggregated cells

Two-step Cell Cycle New, innovative 5 min. Cell Cycle Assay

Cell VitalityCell health determination via the fastest apoptosis assay

What if you knew your cells’ health before they were

dying?

Pre-defined assays (Just press Run!)

Fast and Precise!

NC-Slide™ A2 and A8

NC-Slide A2™

NC-Slide A8™

Fast and Precise!

Only a small sample volume

needed!

10 µl sampleLow volume

Fast measurement

30 µl sampleBetter statistics

Fast and Precise!

Fast and easy to use...

Measure up to 8 viability and cell counts in less than 3 minutesMaintenance, calibration and service free

Simply mix your cells with dye, load and press RUN!

Fast and Precise!

Fast results: Up to 8 viability & cell counts in less than 3 minutes!

Results

Total count Viability Cell diameter Cell Aggregates in % Export to Excel

Validation

21 CFR Part 11 ready IQ/OQ/PQ

Fast and Precise!

Superior Visual Inspection

Objective results!Visual

control

Fast and Precise!

Superior Data Presentation

Browse data from prior samples Easy selection of protocols Wizard for user defined protocols Easy access to application notes Visual inspection of counted cells Easy export of data

Fast and Precise!

Protocol Adaption Wizard

User friendly guide for protocol adaption.Create new protocols for specialized applications.

Fast and Precise!

Superior precision

Cell Conc. (cells/ml)

No. of cells Counted in A2

TheoreticalSD (cells)

TheoreticalCV

5 x 104 560 24 4,2% 1 x 105 1120 33 3,0% 5 x 105 5600 75 1,3% 1 x 106 11200 106 0,9 % 5 x 106 56000 237 0,4% 1 x 107 112000 335 0,3%

Cell Conc. (cells/ml)

No. of cells Counted in A8

TheoreticalSD (cells)

TheoreticalCV

5 x 104 120 11 9,1% 1 x 105 240 15 6,5% 5 x 105 1200 35 2,9% 1 x 106 2400 49 2,0 % 5 x 106 12000 110 0,9% 1 x 107 24000 155 0,6%

Fast and Precise!

Two-Step Cell Cycle Assay

Fast and easy cell cycle assay Directly from T-Flasks or wells (no detachment step) No ethanol fixing No RNase treatment required Standardized Results

Fast, high precision Cell Cycle Assay

Fast and Precise!

Vitality Assay (Apoptosis/Cell Health)

Fast and easy Apoptosis/Cell Health assay For easy detection of changes in levels of reduced thiols In 4 minutes, get 8 Apoptosis measurements.

Find out how healthy your cells

are…

Fast and Precise!

Specifications

Loading volume: 30 µl for NC-Slides A2™, 10 µl for NC-Slides A8™Measurement volume: Viability and Cell Count Assay: 11.2 µl for NC-Slides A2™ and

2.4 µl for NC-Slides A8™. Analysis time: Viability and Cell Count Assay: 8 samples in 3 minutes for NC-Slide A8™. 2 samples in 2 minutes for NC-Slide A2™.Measurement range: Viability and Cell Count Assay: 5 x 103 to 1 x 107 cells/mlOptimal range: Viability and Cell Count Assay: 5 x 104 to 5 x 106 cells/mlSize: 19 x 29 x 32 cm (W x H x D), weight 9 kg Software: NucleoView™ NC-250 computer software for documentation and presentation

Fast and Precise!

• Cell Count• (2-step Viability)

• 1-step Viability and Cell Count

• Vitality

• Cell Cycle

• NC-100, NC-200, NC-250, NC-3000

• NC-3000Apoptosis

GFP

Biomarkers

NC-200, NC-250, NC-3000

NC-250, NC-3000

NC-250, NC-3000

NC-3000

NC-3000

Scalability & flexibilityFunction needs

Product range

Fast and Precise!

Fast and Precise Cell CounterThe NucleoCounter® NC-250™

The right instrument if precision, speed and cell health determination are important factors to you.

990-

0252

ver

. 01.

2013

Precise and objectiveNo manual adjustments, just press RUN

High speed cell count8 samples in less than 3 minutes!

Counting & Analytical AssaysCell health determination via fast apoptosis assay

Low running costsReduce your running costs significantly

Calibration and maintenance freeAlways ready in seconds