FACS Flourescens Activeted Cell sortering

användningsområden

FACS diagram

FACS modell

Hydrodynamisk fokusering

laminärntflöde

Tubulent flöde

Laminärtflöde parametrar

SkumFoam

The relatively stable foam of beer, for example, results from an interaction between a protein fraction rich in basic and aromatic amino acids and the hop bitter compounds. Developing and improving knowledge of the relationship between peptide concentration and structure and foam performance can lead to improved understanding, analysis and control of this complicated property of food products.

Dr. Karl J. Siebert - (Tel: 315-787-2299) Email: Karl_Siebert@Cornell.edu

Ljusspridning - partikelstorlek

Scattering

Forward scatter

Side scatter

http://www.epa.gov/owow/volunteer/proceedings/sixth/session1.html

Side scatter vs Forward scatter

University of Utah

scatterplot

fluorimeteroptik

Vaccine Research Center / 40 Convent Drive / Bethesda, Maryland 20892

Fluorimeter

Flourescens färger

Fluorescein

Fluorescens plott

Lin och log

hoy@cf.ac.uk

Sample : peripheral blood after red cell lysis.Data collected on 10,000 cells.

CD45 FITC (log)

R1=monocytes(CD14+ve) R2=lymphocytes (CD45>monocytes)R3=granulocytes (CD45<monocytes)

CD14

PE (lo

g)

Forward Scatter (linear)

Sid

e Sca

tter

(lin

ear)

Sample : peripheral blood after red cell lysis.Data collected on 10,000

Scatter

Low

High

Events

EventsCD14 PE

CD 45 FITC

Fluorescence

Isometric Displays

hoy@cf.ac.uk

Apoptotiska celler

As cells die or become apoptotic the refractive index of the internal cytoplasm becomes more similar to that of the extracellular medium - this manifests itself as a reduction in forward scatter signal.  At the same time, intracellular changes and invagination of the cytoplasmic membrane lead to an increase in side (or orthogonal or 90°) scatter.  If we add a dead cell discriminatory dye we can identify cells that have become permeable.  In this way we can get low level resolution of dead and apoptotic cells. 

science.cancerresearchuk.org/.../images/51998

endotelceller

Isolation of lymph endothelial cells (LEC) from dermal skin capillary ECs

a case study with cells from one donor pre-selected by CD31 magneto bead

sorting

R2= total cells R1= living cells

www.reliatech.de