f03 salm-camp-eqas-2014 fwd-eurl · eqas 2014 salmonella spp. campylobacter spp. april 24th, 2015...
TRANSCRIPT
EQAS 2014 Salmonella spp.
Campylobacter spp.
April 24th, 2015
Mia Torpdahl ([email protected])Susanne Karlsmose Pedersen ([email protected])
2
Objective
Presentation of obtained results for the EURL-AR and the ECDC FWD network for selected strains and antimicrobials of Salmonella and Campylobacter
Testing performed by: - The public health sector (ECDC FWD network)- The veterinary/food sector (EURL-AR network
Between the networks:- Differing methods applied for determining AMR profile- Differing criteria for categorization as S (I) R
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Included strains and antimicrobialsSalmonella
4 strains: - S-9.1- S-9.2- S-9.7- S-9.8
4 antimicrobials: - Ampicillin (AMP)- Cefotaxime (FOT)- Ciprofloxacin (CIP) (pefloxacin
for disk)- Tetracycline (TET)
Campylobacter
4 strains: - C-9.1- C-9.2- C-9.3- C-9.7
Species ID + 3 antimicrobials: - Species identification (coli/jejuni)- Ciprofloxacin (CIP)- Erythromycin (ERY)- Tetracycline (TET)
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Method for performing AST and ID in the EURL-AR
Salmonella and Campylobacter ASTDemands set in EU/652/2013 (also listed in the protocol):- Dilution methods (broth microdilution or agar dilution)- List of antimicrobials (2 panels for Salmonella)- Range of dilutions- Interpretative criteria
Campylobacter species identification (C. coli, C. jejuni):
- Mandatory- In-house methods accepted- PCR-protocol suggested (available on the EURL-AR website)
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Method for performing AST and ID in the FWD-Net
Salmonella and Campylobacter ASTRecommended EU protocol for harmonised monitoring of antimicrobial resistance in human Salmonella and Campylobacter isolates:- Mandatory (4 Salmonella and 3 Campylobacter) and optional antimicrobials- Dilution methods (broth microdilution or agar dilution) and disk diffusion- Reporting of presently used methods including breakpoints/cutoffs- Reported values interpreted using EUCAST ECOFFs done by SSI for all
participants
Campylobacter species identification (C. coli, C. jejuni):
- Mandatory- In-house methods accepted
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EURL-AR Overall results (AST)
2/560
23/348
0
1
2
3
4
5
6
7
8
9
10
Salmonella Campylobacter
Dev
iatio
n le
vel(
%)
7
FWD-Net Overall results (AST)
8
EURL-AR Salmonella results – pr. lab
0
2
4
6
8
10
12
14
58 1 2 4 6 9 11 12 13 16 17 18 19 20 21 22 23 25 26 29 30 32 33 34 36 37 38 39 40 41 42 45 56 57 59
Dev
iatio
n le
vel(
%)
Deviation % Acceptance level
Laboratory number
Lab #58: 12.5% deviations based on 2 deviations out of 16 results
9
FWD-Net Salmonella results – pr. lab – 31 labs
10
EURL-AR Salmonella results – antimicrobials
N=1
40
N=1
40
N=1
40
N=1
40
0
1
2
3
4
5
Dev
iatio
n le
vel (
%)
11
FWD-Net Salmonella results – antimicrobials
12
EURL-AR Salmonella results – strains
0
1
2
3
4
5D
evia
tion
leve
l (%
)
13
FWD-Net Salmonella results – strains
EURL-AR Campylobacter results – pr. lab
14
0
5
10
15
20
25
30
35
40
45
50
29 40 34 42 36 1 4 19 30 39 41 59 2 6 9 11 12 14 17 18 20 21 22 23 25 26 32 33 37 45 56 58
Dev
iatio
n le
vel (
%)
Deviation % Acceptance level
Laboratory number
4/9
4/12
2/12
3/12
1/12
Defined as outliers (full set of results)
FWD-Net Campylobacter results – pr. lab – 26 labs
15
16
EURL-AR Campylobacter results – antimicrobials
6/124
8/124 9/123
0123456789
10
Dev
iatio
n le
vel(
%)
17
FWD-Net Campylobacter results – antimicrobials
18
EURL-AR Campylobacter results – strains
2/96
9/92
6/93 6/90
0123456789
101112
Dev
iatio
n le
vel (
%)
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FWD-Net Campylobacter results – strains
FWD-NetIn total; 10 deviations in 104 tests (9,6% deviation)- C-9.1 (exp. C. jejuni) L001, - C-9.2 (exp. C. coli) L001, L015, L036- C-9.3 (exp. C. coli) L001, L015, L022. L025- C-9.7 (exp. C. jejuni) L001, L036
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Obtained results (species ID, Campylobacter)
EURL-ARIn total; 6 deviation in 128 tests (4.7% deviation)- C-9.1 (exp. C. jejuni) lab #36- C-9.2 (exp. C. coli) lab #40- C-9.7 (exp. C. jejuni) lab #19, #36, #40, #42
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Summing up
Challenges, Salmonella- Due to lack of tests in the whole range of antimicrobial dilutions, one
laboratory’s results were excluded from the analysis (EURL-AR)- Due to lack of tests in the whole range of antimicrobial dilutions,
several laboratory results were excluded from the analysis (FWD)
Challenges, Campylobacter- Two laboratories identified as outliers (EURL-AR)- One laboratories identified as outlier (FWD)- Due to lack of tests in the whole range of antimicrobial dilutions, one
laboratory’s results were excluded from the analysis (EURL-AR)- Species identification (EURL-AR and FWD)