exploring a putative gene
DESCRIPTION
Exploring a Putative Gene. גיא וקסמן. רועי נבון. Problem definition. Taking one of the thousands of Putative genes that were “found” In the human genome project & Trying to determine whether this Sequence might be a real gene that can be translated into a protein product. - PowerPoint PPT PresentationTRANSCRIPT
Exploring a Putative Gene
גיא וקסמןרועי נבון
Problem definition
Taking one of the thousands of Putative genes that were “found”
In the human genome project & Trying to determine whether this Sequence might be a real gene that canbe translated into a protein product.
The Selected GeneThe Selected Gene::
NM_014727
Homo sapiens myeloid/lymphoid or mixed-lineage leukemia 4 (MLL4).
PSI-BLAST
Many entrances are actually on thesame locus (SNPs, flanking, different
Alleles etc .)`
The Locus problemThe Locus problem
Different Entrances on the Same Different Entrances on the Same Chromosomal RegionChromosomal Region
Conserved DomainsConserved Domains
SET1
CXXC zinc finger
Zinc finger
2-BLAST between the putative MLL4 gene & the most similar real Gene-Trithorax, from DrosophilaMelanogaster.
E=5e-87
E=2e-82
22--BLASTBLAST
Multiple AlignmentMultiple Alignment
SET1 domain
3D Structure – 3D Structure – Zinc Finger DomainZinc Finger Domain
Biological AspectsBiological AspectsDrosophila melanogaster
The trithorax gene, a trans-acting regulator of the bithorax complex in Drosophila, encodes a protein with zinc-binding domains.
Mazo AM, Huang DH, Mozer BA, Dawid IB
ככה נראיתי פעם...
Homo Sapians
Acute mixed-lineage leukemia t(4;11)(q21;q23) generates an MLL-AF4 fusion product
ConclusionsConclusions
1 .We found a high similarity between our putative gene and genes from other
species.
2 .The MLL4 putative gene contains at least 3
conserved domains .
3 .The homologue genes from Drosophila and
Human are associated with the nervous system and leukemia respectively .
Possible directions for Possible directions for future researchfuture research
1 .Trying to find DNA motifs which bind to our putative gene and to its homologues.
2 .Trying to isolate the full length protein .
3 .When 3D structure prediction will be available, we’ll try to compare our putative
protein with other known proteins.