explore critical interactions with reichert surface ... · reichert surface plasmon resonance (spr)...
TRANSCRIPT
Reichert Surface Plasmon Resonance (SPR) Systems provide robust, reliable precisioninstruments to investigate molecular interactions in real time without the need tolabel molecules.
We are dedicated to serving the diverse interests of academia and industry byproviding the most flexible SPR platform available in today’s marketplace at anunbeatable price. Our systems allow for a wide range of applications and are easilymodified to meet different experimental requirements.
Unlimited Possibilities for Breakthrough Work
With more than a decade of experience in SPR technology,Reichert Life Sciences is happy to discuss your SPR applications,arrange for demonstrations, and configure systems to meetyour research needs.
Explore Critical Interactions with Reichert Surface Plasmon Resonance Technology
ReichertSPR Measures:• Kinetics• Affinity/Equilibrium• Thermodynamics• Concentration • Adsorption rates
ReichertSPR Answers:• Is there an interaction?• How fast?• How strong?• How long?• How much?• How specific?
ReichertSPR Flexibility:Combine Reichert SPR with:
• Electrochemistry• Fluorescence• Photochemistry
Couple Reichert SPR with:• Mass Spectrometry• Liquid Chromatography
Modular, component-basedsystem.Highly flexible. Excellent value.
Surface Plasmon Resonance Systems
Corporate HeadquartersReichert, Inc.3362 Walden AvenueBuffalo, New York 14043USATel: +1 716-686-4500Fax: +1 716-686-4555Toll Free USA: 1-888-849-8955
European HeadquartersReichert Instruments GmbHHubertusstrasse 2D-82229 SeefeldGermanyTel: +49 8152 99 3530Fax: +49 8152 99 35311
e-mail: [email protected]
Innovative precision instruments for over a century
Innovative precision instruments for over a century
• Surfaces• Polymers• Proteins•Antibody/Antigen• Nucleic Acids• Carbohydrates• Lipids• Small Molecules• Drugs• Bacteria• Viruses
BASELINE ASSOCIATION DISSOCIATION REGENERATION
RESP
ONSE
RESP
ONSE
RESP
ONSE
RESP
ONSE
TIME TIME TIME TIME
Label-free Molecular Interaction Analysis
Reichert Surface Plasmon Resonance (SPR) Systems provide robust, reliable precisioninstruments to investigate molecular interactions in real time without the need tolabel molecules.
We are dedicated to serving the diverse interests of academia and industry byproviding the most flexible SPR platform available in today’s marketplace at anunbeatable price. Our systems allow for a wide range of applications and are easilymodified to meet different experimental requirements.
Unlimited Possibilities for Breakthrough Work
With more than a decade of experience in SPR technology,Reichert Life Sciences is happy to discuss your SPR applications,arrange for demonstrations, and configure systems to meetyour research needs.
Explore Critical Interactions with Reichert Surface Plasmon Resonance Technology
ReichertSPR Measures:• Kinetics• Affinity/Equilibrium• Thermodynamics• Concentration • Adsorption rates
ReichertSPR Answers:• Is there an interaction?• How fast?• How strong?• How long?• How much?• How specific?
ReichertSPR Flexibility:Combine Reichert SPR with:
• Electrochemistry• Fluorescence• Photochemistry
Couple Reichert SPR with:• Mass Spectrometry• Liquid Chromatography
Modular, component-basedsystem.Highly flexible. Excellent value.
Surface Plasmon Resonance Systems
Corporate HeadquartersReichert, Inc.3362 Walden AvenueBuffalo, New York 14043USATel: +1 716-686-4500Fax: +1 716-686-4555Toll Free USA: 1-888-849-8955
European HeadquartersReichert Instruments GmbHHubertusstrasse 2D-82229 SeefeldGermanyTel: +49 8152 99 3530Fax: +49 8152 99 35311
e-mail: [email protected]
Innovative precision instruments for over a century
Innovative precision instruments for over a century
• Surfaces• Polymers• Proteins•Antibody/Antigen• Nucleic Acids• Carbohydrates• Lipids• Small Molecules• Drugs• Bacteria• Viruses
BASELINE ASSOCIATION DISSOCIATION REGENERATION
RESP
ONSE
RESP
ONSE
RESP
ONSE
RESP
ONSE
TIME TIME TIME TIME
Label-free Molecular Interaction Analysis
Reichert Surface Plasmon Resonance (SPR) Systems provide robust, reliable precisioninstruments to investigate molecular interactions in real time without the need tolabel molecules.
We are dedicated to serving the diverse interests of academia and industry byproviding the most flexible SPR platform available in today’s marketplace at anunbeatable price. Our systems allow for a wide range of applications and are easilymodified to meet different experimental requirements.
Unlimited Possibilities for Breakthrough Work
With more than a decade of experience in SPR technology,Reichert Life Sciences is happy to discuss your SPR applications,arrange for demonstrations, and configure systems to meetyour research needs.
Explore Critical Interactions with Reichert Surface Plasmon Resonance Technology
ReichertSPR Measures:• Kinetics• Affinity/Equilibrium• Thermodynamics• Concentration • Adsorption rates
ReichertSPR Answers:• Is there an interaction?• How fast?• How strong?• How long?• How much?• How specific?
ReichertSPR Flexibility:Combine Reichert SPR with:
• Electrochemistry• Fluorescence• Photochemistry
Couple Reichert SPR with:• Mass Spectrometry• Liquid Chromatography
Modular, component-basedsystem.Highly flexible. Excellent value.
Surface Plasmon Resonance Systems
Corporate HeadquartersReichert, Inc.3362 Walden AvenueBuffalo, New York 14043USATel: +1 716-686-4500Fax: +1 716-686-4555Toll Free USA: 1-888-849-8955
European HeadquartersReichert Instruments GmbHHubertusstrasse 2D-82229 SeefeldGermanyTel: +49 8152 99 3530Fax: +49 8152 99 35311
e-mail: [email protected]
Innovative precision instruments for over a century
Innovative precision instruments for over a century
• Surfaces• Polymers• Proteins•Antibody/Antigen• Nucleic Acids• Carbohydrates• Lipids• Small Molecules• Drugs• Bacteria• Viruses
BASELINE ASSOCIATION DISSOCIATION REGENERATION
RESP
ONSE
RESP
ONSE
RESP
ONSE
RESP
ONSE
TIME TIME TIME TIME
Label-free Molecular Interaction Analysis
Flexible Flow Cell Design The Reichert SPR systems provide flexibility in experimentaldesign with their exclusive collection of flow cell designs.
The instruments can be configured with any of the flowcells to carry out SPR in combination with othermeasurements. And Reichert’s sensor slides are available inpure gold or with a variety of surface chemistries.
Other advantages include:• Series or parallel flow • Different flow cell modules for
combining SPR with electrochemistry, photochemistry,and fluorescence
• Combine Reichert SPR with mass spectrometry and liquid chromotography
• Sapphire prism provideshigh thermal conductivity
• Stop flow capability
SPR InstrumentsReichert’s SPR instruments are powerful research toolsoffering a high level of flexibility, outstandingperformance, and remarkable affordability. The SR7000offers critical angle capability in a single channelinstrument while the SR7000DC features simultaneoustwo-channel measurements, with user definable flowconfigurations.
Both models provide:• Virtually indestructible sapphire prism • Excellent baseline stability• Extremely low noise• Temperature control from 100 C below
ambient to 900 C• Broad refractive index range (1.32 to 1.52)• High speed scanning for fast kinetics• Full access to reflectivity data• Small footprint in the lab• Variety of sensor slides
Thanks to their modular/component-based design, Reichert SPR systems offer the most flexibleplatform to date. Researchers can target specific applications by configuring the most appropriatesystem. As a scientific research partner and teaching tool, Reichert SPR offers outstanding valuewith low maintenance at an affordable price.
Programmable Syringe Pump A precision syringe pump isessential for optimum performance.The Reichert SR7500 syringe pumpoffers high precision flow with anextremely wide flow rate range tosuit the application, e.g. slow flowfor equilibrium measurements orfast flow for mass-transport limitedapplications. The pump can also
aspirate extremely small sample volumes into the flow celland can pump forward/reverse very quickly to mimic constantflow conditions. This provides the capability to immobilize amolecule of interest despite limited sample volumes.
• Wide flow rate range (0.1 to 3000 μl/min)• Automated refills• Different sized syringes (0.5, 1, 5 ml)• Forward/reverse – push/pull• Software control
SPR
Diverter Valve This valve allows the flow to beswitched between one channelonly or to series, allowingautomated immobilization/captureof ligand onto only one channel.
Flexible Fluidics PlatformThe Reichert SPR system is designed to use low-cost, off-the-shelf HPLC fittings and tubing, allowing for easy changeover.The system accommodates a wide range of samplecompositions (from salt solution to cell lysates), varyingviscosities, and particulate matter. The tubing is compatiblewith an array of solvents and easy to replace if damaged,blocked, or clogged.
• HPLC structure/setup• Tubing from 64 to 500μm inner diameter
• Variable loop size - 10 to 5000 μl
• Parallel or series flow• Compatible with a
wide range of solventsand samples
Computer andSoftware The Reichert SPR instrument has a single GUI interface thatintegrates all system components from operation throughdata collection. It has the ability to interface with populardata analysis programs such as Scrubber and Clamp.
Reichert SPRA new generation of sophisticated tools for
cutting edge research and scientific discovery
• High flexibility• Upgrade/Add components anytime Injecting the Samples
Reichert offers several methods to manage sampleinjection. These include an autosampler and a semi-automatic injection valve. The systems are easily adapted to accommodate other injection methods.
The Reichert Robotic Autosampler is designed tomaximize the performance and throughput of the SPRinstrument. Easy to operate and maintain, the autosamplerautomatically injects your samples while you do otherwork—or leave the lab—allowing for 24 hours ofunattended analysis time.
• Automatic execution of experimental protocol• Inject from two 384- or 96- well plates or
two 48- vial trays• Sample cooling to 40 C
The Reichert Semi-AutomaticValve is electronically actuated andcomputer-controlled throughReichert’s Data Acquisition software,providing extremely uniform sampleinjections. The sample loop is manually filled, but the computer
controls the injection time by automatically switching theinjection valve.
• Easy to choose any loop size• Small footprint
Real-time, High Quality, Publication-Ready Data
The high sensitivity and low noise characteristics of Reichert SPR systems provide high quality kinetic and equilibrium data for interactions of interest. Easy-to-use data acquisition software makes it simple to set up instrument operating parameters,monitor/record experiment data in real time, and efficiently save/analyze data.
Open architecture Adapts to your research
• Minimal maintenance• Low operating costs
• Small footprint• Remarkable value
Flexible Flow Cell Design The Reichert SPR systems provide flexibility in experimentaldesign with their exclusive collection of flow cell designs.
The instruments can be configured with any of the flowcells to carry out SPR in combination with othermeasurements. And Reichert’s sensor slides are available inpure gold or with a variety of surface chemistries.
Other advantages include:• Series or parallel flow • Different flow cell modules for
combining SPR with electrochemistry, photochemistry,and fluorescence
• Combine Reichert SPR with mass spectrometry and liquid chromotography
• Sapphire prism provideshigh thermal conductivity
• Stop flow capability
SPR InstrumentsReichert’s SPR instruments are powerful research toolsoffering a high level of flexibility, outstandingperformance, and remarkable affordability. The SR7000offers critical angle capability in a single channelinstrument while the SR7000DC features simultaneoustwo-channel measurements, with user definable flowconfigurations.
Both models provide:• Virtually indestructible sapphire prism • Excellent baseline stability• Extremely low noise• Temperature control from 100 C below
ambient to 900 C• Broad refractive index range (1.32 to 1.52)• High speed scanning for fast kinetics• Full access to reflectivity data• Small footprint in the lab• Variety of sensor slides
Thanks to their modular/component-based design, Reichert SPR systems offer the most flexibleplatform to date. Researchers can target specific applications by configuring the most appropriatesystem. As a scientific research partner and teaching tool, Reichert SPR offers outstanding valuewith low maintenance at an affordable price.
Programmable Syringe Pump A precision syringe pump isessential for optimum performance.The Reichert SR7500 syringe pumpoffers high precision flow with anextremely wide flow rate range tosuit the application, e.g. slow flowfor equilibrium measurements orfast flow for mass-transport limitedapplications. The pump can also
aspirate extremely small sample volumes into the flow celland can pump forward/reverse very quickly to mimic constantflow conditions. This provides the capability to immobilize amolecule of interest despite limited sample volumes.
• Wide flow rate range (0.1 to 3000 μl/min)• Automated refills• Different sized syringes (0.5, 1, 5 ml)• Forward/reverse – push/pull• Software control
SPR
Diverter Valve This valve allows the flow to beswitched between one channelonly or to series, allowingautomated immobilization/captureof ligand onto only one channel.
Flexible Fluidics PlatformThe Reichert SPR system is designed to use low-cost, off-the-shelf HPLC fittings and tubing, allowing for easy changeover.The system accommodates a wide range of samplecompositions (from salt solution to cell lysates), varyingviscosities, and particulate matter. The tubing is compatiblewith an array of solvents and easy to replace if damaged,blocked, or clogged.
• HPLC structure/setup• Tubing from 64 to 500μm inner diameter
• Variable loop size - 10 to 5000 μl
• Parallel or series flow• Compatible with a
wide range of solventsand samples
Computer andSoftware The Reichert SPR instrument has a single GUI interface thatintegrates all system components from operation throughdata collection. It has the ability to interface with populardata analysis programs such as Scrubber and Clamp.
Reichert SPRA new generation of sophisticated tools for
cutting edge research and scientific discovery
• High flexibility• Upgrade/Add components anytime Injecting the Samples
Reichert offers several methods to manage sampleinjection. These include an autosampler and a semi-automatic injection valve. The systems are easily adapted to accommodate other injection methods.
The Reichert Robotic Autosampler is designed tomaximize the performance and throughput of the SPRinstrument. Easy to operate and maintain, the autosamplerautomatically injects your samples while you do otherwork—or leave the lab—allowing for 24 hours ofunattended analysis time.
• Automatic execution of experimental protocol• Inject from two 384- or 96- well plates or
two 48- vial trays• Sample cooling to 40 C
The Reichert Semi-AutomaticValve is electronically actuated andcomputer-controlled throughReichert’s Data Acquisition software,providing extremely uniform sampleinjections. The sample loop is manually filled, but the computer
controls the injection time by automatically switching theinjection valve.
• Easy to choose any loop size• Small footprint
Real-time, High Quality, Publication-Ready Data
The high sensitivity and low noise characteristics of Reichert SPR systems provide high quality kinetic and equilibrium data for interactions of interest. Easy-to-use data acquisition software makes it simple to set up instrument operating parameters,monitor/record experiment data in real time, and efficiently save/analyze data.
Open architecture Adapts to your research
• Minimal maintenance• Low operating costs
• Small footprint• Remarkable value
Flexible Flow Cell Design The Reichert SPR systems provide flexibility in experimentaldesign with their exclusive collection of flow cell designs.
The instruments can be configured with any of the flowcells to carry out SPR in combination with othermeasurements. And Reichert’s sensor slides are available inpure gold or with a variety of surface chemistries.
Other advantages include:• Series or parallel flow • Different flow cell modules for
combining SPR with electrochemistry, photochemistry,and fluorescence
• Combine Reichert SPR with mass spectrometry and liquid chromotography
• Sapphire prism provideshigh thermal conductivity
• Stop flow capability
SPR InstrumentsReichert’s SPR instruments are powerful research toolsoffering a high level of flexibility, outstandingperformance, and remarkable affordability. The SR7000offers critical angle capability in a single channelinstrument while the SR7000DC features simultaneoustwo-channel measurements, with user definable flowconfigurations.
Both models provide:• Virtually indestructible sapphire prism • Excellent baseline stability• Extremely low noise• Temperature control from 100 C below
ambient to 900 C• Broad refractive index range (1.32 to 1.52)• High speed scanning for fast kinetics• Full access to reflectivity data• Small footprint in the lab• Variety of sensor slides
Thanks to their modular/component-based design, Reichert SPR systems offer the most flexibleplatform to date. Researchers can target specific applications by configuring the most appropriatesystem. As a scientific research partner and teaching tool, Reichert SPR offers outstanding valuewith low maintenance at an affordable price.
Programmable Syringe Pump A precision syringe pump isessential for optimum performance.The Reichert SR7500 syringe pumpoffers high precision flow with anextremely wide flow rate range tosuit the application, e.g. slow flowfor equilibrium measurements orfast flow for mass-transport limitedapplications. The pump can also
aspirate extremely small sample volumes into the flow celland can pump forward/reverse very quickly to mimic constantflow conditions. This provides the capability to immobilize amolecule of interest despite limited sample volumes.
• Wide flow rate range (0.1 to 3000 μl/min)• Automated refills• Different sized syringes (0.5, 1, 5 ml)• Forward/reverse – push/pull• Software control
SPR
Diverter Valve This valve allows the flow to beswitched between one channelonly or to series, allowingautomated immobilization/captureof ligand onto only one channel.
Flexible Fluidics PlatformThe Reichert SPR system is designed to use low-cost, off-the-shelf HPLC fittings and tubing, allowing for easy changeover.The system accommodates a wide range of samplecompositions (from salt solution to cell lysates), varyingviscosities, and particulate matter. The tubing is compatiblewith an array of solvents and easy to replace if damaged,blocked, or clogged.
• HPLC structure/setup• Tubing from 64 to 500μm inner diameter
• Variable loop size - 10 to 5000 μl
• Parallel or series flow• Compatible with a
wide range of solventsand samples
Computer andSoftware The Reichert SPR instrument has a single GUI interface thatintegrates all system components from operation throughdata collection. It has the ability to interface with populardata analysis programs such as Scrubber and Clamp.
Reichert SPRA new generation of sophisticated tools for
cutting edge research and scientific discovery
• High flexibility• Upgrade/Add components anytime Injecting the Samples
Reichert offers several methods to manage sampleinjection. These include an autosampler and a semi-automatic injection valve. The systems are easily adapted to accommodate other injection methods.
The Reichert Robotic Autosampler is designed tomaximize the performance and throughput of the SPRinstrument. Easy to operate and maintain, the autosamplerautomatically injects your samples while you do otherwork—or leave the lab—allowing for 24 hours ofunattended analysis time.
• Automatic execution of experimental protocol• Inject from two 384- or 96- well plates or
two 48- vial trays• Sample cooling to 40 C
The Reichert Semi-AutomaticValve is electronically actuated andcomputer-controlled throughReichert’s Data Acquisition software,providing extremely uniform sampleinjections. The sample loop is manually filled, but the computer
controls the injection time by automatically switching theinjection valve.
• Easy to choose any loop size• Small footprint
Real-time, High Quality, Publication-Ready Data
The high sensitivity and low noise characteristics of Reichert SPR systems provide high quality kinetic and equilibrium data for interactions of interest. Easy-to-use data acquisition software makes it simple to set up instrument operating parameters,monitor/record experiment data in real time, and efficiently save/analyze data.
Open architecture Adapts to your research
• Minimal maintenance• Low operating costs
• Small footprint• Remarkable value
Reichert Surface Plasmon Resonance (SPR) Systems provide robust, reliable precisioninstruments to investigate molecular interactions in real time without the need tolabel molecules.
We are dedicated to serving the diverse interests of academia and industry byproviding the most flexible SPR platform available in today’s marketplace at anunbeatable price. Our systems allow for a wide range of applications and are easilymodified to meet different experimental requirements.
Unlimited Possibilities for Breakthrough Work
With more than a decade of experience in SPR technology,Reichert Life Sciences is happy to discuss your SPR applications,arrange for demonstrations, and configure systems to meetyour research needs.
Explore Critical Interactions with Reichert Surface Plasmon Resonance Technology
ReichertSPR Measures:• Kinetics• Affinity/Equilibrium• Thermodynamics• Concentration • Adsorption rates
ReichertSPR Answers:• Is there an interaction?• How fast?• How strong?• How long?• How much?• How specific?
ReichertSPR Flexibility:Combine Reichert SPR with:
• Electrochemistry• Fluorescence• Photochemistry
Couple Reichert SPR with:• Mass Spectrometry• Liquid Chromatography
Modular, component-basedsystem.Highly flexible. Excellent value.
Surface Plasmon Resonance Systems
Corporate HeadquartersReichert, Inc.3362 Walden AvenueBuffalo, New York 14043USATel: +1 716-686-4500Fax: +1 716-686-4555Toll Free USA: 1-888-849-8955
European HeadquartersReichert Instruments GmbHHubertusstrasse 2D-82229 SeefeldGermanyTel: +49 8152 99 3530Fax: +49 8152 99 35311
e-mail: [email protected]
Innovative precision instruments for over a century
Innovative precision instruments for over a century
• Surfaces• Polymers• Proteins•Antibody/Antigen• Nucleic Acids• Carbohydrates• Lipids• Small Molecules• Drugs• Bacteria• Viruses
BASELINE ASSOCIATION DISSOCIATION REGENERATION
RESP
ONSE
RESP
ONSE
RESP
ONSE
RESP
ONSE
TIME TIME TIME TIME
Label-free Molecular Interaction Analysis