experiments of hplc · 2013. 12. 19. · bioprocess lab. hplc - high performance liquid...

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Bioprocess lab. Experiments of HPLC

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Page 1: Experiments of HPLC · 2013. 12. 19. · Bioprocess lab. HPLC - High performance liquid chromatography. - Rapid separation method. - Variety of mobile phase and stationary phase

Bioprocess lab.

Experiments of HPLC

Page 2: Experiments of HPLC · 2013. 12. 19. · Bioprocess lab. HPLC - High performance liquid chromatography. - Rapid separation method. - Variety of mobile phase and stationary phase

Bioprocess lab.

개 요

Chromatography

Gas Liquid

GSC GLC Column

HPLC

TLCPaper

Page 3: Experiments of HPLC · 2013. 12. 19. · Bioprocess lab. HPLC - High performance liquid chromatography. - Rapid separation method. - Variety of mobile phase and stationary phase

Bioprocess lab.

HPLC- High performance liquid chromatography.- Rapid separation method.- Variety of mobile phase and stationary

phase.

Page 4: Experiments of HPLC · 2013. 12. 19. · Bioprocess lab. HPLC - High performance liquid chromatography. - Rapid separation method. - Variety of mobile phase and stationary phase

Bioprocess lab.

크로마토그래피의 분리의 원리

Page 5: Experiments of HPLC · 2013. 12. 19. · Bioprocess lab. HPLC - High performance liquid chromatography. - Rapid separation method. - Variety of mobile phase and stationary phase

Bioprocess lab.

크로마토그래피의 도식

Detector

Recorder

Chromatogram

M.P.

Pump

I. P.

COLUMN

Page 6: Experiments of HPLC · 2013. 12. 19. · Bioprocess lab. HPLC - High performance liquid chromatography. - Rapid separation method. - Variety of mobile phase and stationary phase

Bioprocess lab.

Analitical result from HPLC

Page 7: Experiments of HPLC · 2013. 12. 19. · Bioprocess lab. HPLC - High performance liquid chromatography. - Rapid separation method. - Variety of mobile phase and stationary phase

Bioprocess lab.

Experiments

1. 이동상 제조

Page 8: Experiments of HPLC · 2013. 12. 19. · Bioprocess lab. HPLC - High performance liquid chromatography. - Rapid separation method. - Variety of mobile phase and stationary phase

Bioprocess lab.

2. Filtration and degassing

Page 9: Experiments of HPLC · 2013. 12. 19. · Bioprocess lab. HPLC - High performance liquid chromatography. - Rapid separation method. - Variety of mobile phase and stationary phase

Bioprocess lab.

3. 펌프내부기포제거

Page 10: Experiments of HPLC · 2013. 12. 19. · Bioprocess lab. HPLC - High performance liquid chromatography. - Rapid separation method. - Variety of mobile phase and stationary phase

Bioprocess lab.

4. Column확인

Page 11: Experiments of HPLC · 2013. 12. 19. · Bioprocess lab. HPLC - High performance liquid chromatography. - Rapid separation method. - Variety of mobile phase and stationary phase

Bioprocess lab.

5. Pump 압력확인

Page 12: Experiments of HPLC · 2013. 12. 19. · Bioprocess lab. HPLC - High performance liquid chromatography. - Rapid separation method. - Variety of mobile phase and stationary phase

Bioprocess lab.

6. Column 누수확인

Page 13: Experiments of HPLC · 2013. 12. 19. · Bioprocess lab. HPLC - High performance liquid chromatography. - Rapid separation method. - Variety of mobile phase and stationary phase

Bioprocess lab.

7. 온도확인

Page 14: Experiments of HPLC · 2013. 12. 19. · Bioprocess lab. HPLC - High performance liquid chromatography. - Rapid separation method. - Variety of mobile phase and stationary phase

Bioprocess lab.

8. UV Detector

Page 15: Experiments of HPLC · 2013. 12. 19. · Bioprocess lab. HPLC - High performance liquid chromatography. - Rapid separation method. - Variety of mobile phase and stationary phase

Bioprocess lab.

9. Sample preparation

Page 16: Experiments of HPLC · 2013. 12. 19. · Bioprocess lab. HPLC - High performance liquid chromatography. - Rapid separation method. - Variety of mobile phase and stationary phase

Bioprocess lab.

10. Sample filtration

Page 17: Experiments of HPLC · 2013. 12. 19. · Bioprocess lab. HPLC - High performance liquid chromatography. - Rapid separation method. - Variety of mobile phase and stationary phase

Bioprocess lab.

11. Injection