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from theory. Progress in regard to it has thus been made b3long but even steps, each independent observation being con.firmatory of what has passed before, and tending ultimatelyto the same end.

2nd. There can be no escaping at this moment from thetheory that ozone is a modified condition of oxygen-in-deed, is oxygen plus force, which force is probably used incondensation; in other words, the power or capability of oxygento combine with itself.

3rd. The artificial production of ozone, and the quantitythat can be so produced, or, perhaps it is preferable to say, thequantity of oxygen that can be transformed into ozone, is mostclearly and distinctly defined-in fact, in the laboratory, theproduction of ozone is an absolute verity, as pure as the pro-duction of oxygen or chlorine. How ozone is engendered inthe air-if it be there-is not yet in any way definitely under-stood.

4th. The ordinary tests for ozone are imperfect, not so muchbecause they will not always prove the presence of ozone, butbecause they prove too much-that is to say, the presence ofother bodies also common to the atmosphere.

5the. In its action on the body the effects of ozone seem tobe confined to the respiratory passages and structures; indeed,it is purely local in its action, closely resembling diluted chlo-rine and diluted bromine in vapour. The symptoms induced,varying in intensity, may be catarrhal, bronchial, or pneu-monic ; nor is there any evidence of any other class of diseasesfrom ozone.

6th. Ozone administered so as to produce death promotesconditions clearly indicative, as I have already stated, of localaction confined to the pulmonary system; the conditions arethose of suppressed secretion, vascular- congestion, and exu-dation.

7th. The relation of ozone to disease-if there be such a re-lationship-is confined to disease local in its character, and toone part of the system, the respiratory. Beyond this thereis no logical argument whatever; and even this must be ac-cepted as problematical until other potent influences are esti-mated as causes, on the principle of exclusion.

8th. On dead organic matter ozone exerts a powerfully de-structive action, resembling, in this way, chlorine, iodine, andespecially bromine.

9th. Ozone is a disinfectant and deodoriser, belonging tothose bodies which disinfect and deodorise by resolving anddecomposing into primitive and innocuous forms ; competing,in this respect, with the substances already named-chlorine,bromine, and iodine. It possesses these qualities in a less de-gree than chlorine and bromine, and is in many cases not so iapplicable as iodine. * -j’, " I

10th. As a preventive of disease, ozone can only act by de- Istroying organic animal poisons, in which respect it may againbe compared with the substances I have more than oncenamed.

Lastly, as a remedy. In the form of ozonised oil, of ozonisedether, and ozonised water, it once more ranks with a similarcombination of remedies containing chlorine, bromine, and

especially iod;ne. Whether, in any respect, it may prove tohave greater advantages than the last-named trusty and readyagent, can only be conclusively arrived at by determiningwhether it will do what iodine will not do; and this can onlybe decisively made out by applying to it the touchstone of in-ductive philosophy-a rigid exclusion of all that is ineffective.

Note. -Under the head ’’ Tests," in the portion of this paperwhich appeared last week, the following correction should bemade :-Instead of "There are objections to the ozone box,which objections have been well pointed out by Mr. JohnSmyth, who thinks that," &c.,-it should read thus: Thereare objections to the ozone box, which objections have been wellpointed out by Mr. John Smyth. This observer takes a viewdissimilar to that of Lowe, who thinks that the velocity ofair, &c.

StafFord, Jan. 1863.

DEATH OF 112. LABORIE. - This well-known and

highly-esteemed surgeon is another victim to the risks inherentin our profession. He had the charge of the establishmentfor convalescents at Vincennes, near Paris, and took erysipelasfrom one of his patients. This soon assumed the gangrenoustype, and M. Laborie was carried off, having hardly reachedhis fifty-fifth year. A great concourse of frienus and membersof the profession accompanied his remains to the grave ; andfrom the discourses delivered it may be gathered that the Ideceased is universally regretted.

EXPERIMENTS CONNECTED WITH SMALL-

POX, INOCULATION, & VACCINATION.

BY JOHN SHORTT, M.D., F.L.S., M.R.C.P.L., &c.,GENERAL SUPERINTENDENT OF VACCINATION, MADRAS PRESIDENCY.

(Continued from p. 47.)

Third Series of Cases. (Raneepett Parcherry.) )Nov. 10th.-Moothoo, aged sixteen, male, caste Pariah. Said

to have had fever on the 2nd Nov., and on the fourth morningthe eruption made its appearance ; few, not exceeding 200 to300 in number, about the size of coriander seeds, firm, withcentral depressions, and having a flattened appearance, and ofa pearl-like colour. When ruptured a clear, transparent fluidoozed out slowly; some sickening odour about the patient; freefrom fever at present. The fluid taken from several eruptionswas inserted on the inner surface of the ears and scrotum of ablack bull, aged two years, and another jenny (ass) was ope-rated on with the same matter. The insertions in this latterinstance were made on the outer side of each nostril by fourpunctures.

1 lth.-Insertions in the bull look angry; some indications ofabdominal glands swelling.

12th.—Punctures forming ; distinctly visible in a papularform, giving a gritty feel to the finger, whilst the centres of thepapules are depressed.

13 th. -Papules fully formed, with raised margins and centraldepressions at the insertions only. These were ruptured, andthe contents transferred to two subjects this day.

’ No. 1.-Bejana Bee, aged ten, female ; Mahomedan.No. 2. -Beeban Bee, aged three, female ; ditto.

No. 1.Nov. 15th (third day).-All six insertions are promising.16th (fourth day).-Three punctures on the right arm, ele-

vated above the skin, and can be distinctly seen.17th (fifth day).-Only two punctures of the left arm suc-

ceeded ultimately, and are now visible.19th (seventh day).-Two very small vesicles in left arm.20th (eighth day).-Third insertion in left arm shows indi-

cations of rising.21st (ninth day).-Vesicles appear like ordinary vaccine.

These were ruptured, and the matter transferred to two othersubjects. Patient complains of pain in the eyes and extremi-ties, and is somewhat feverish now.22nd (tenth day).-The vesicles broken yesterday are crust-

ing ; those on the right arm more papular in appearance ; hasfever and headache; pains in arms and a,xiUa3.

23rd (eleventh day). -Eruptions on left arm scabbing; rightarm vesicular; these were broken, and a capillary tube chargedwith contents.25th (thirteenth day).-Free from fever; a few small pimples.

are scattered over the wrists and trunk of this patient.No. 2.

Nov. 16th (fourth day).-Insertions slightly elevated.17th (fifth day).-Two in the right and one in the left arm

are raised above the skin.19th (seventh day).-At present there are only two vesicles’

promising and visible in the left arm.20th (eighth day).-Of the insertions in the left arm, one is

small and of a conical form, the other two are papular anddo not promise well.

21st (ninth day).-Vesicles in the left arm promising;feverish.

23rd (eleventh day).-High fever; fidgety; hands tremu-lous ; papules rising.

25th (thirteenth day).-The face, trunk, and extremitiescovered with from 200 to 300 small-pox eruptions.

Dec. 1st (nineteenth day).-Face and extremities are scab-bing. Eruptions about the extremities pustular. These were

ruptured, and the contents transferred to a cow-calf, agedeighteen months.In all there were four small-pox subjects, from whom virus

was transferred to the following animals :-Cattle :* Two young bulls, three heifers, and one cow.

One pony. One dog.Two donkeys. One goat.

* Vide tabular statement attached.

127

From these cattle there were five successive transfers of the also scabbed, and a few dropped off, whilst other few passedvirus to the human subject. The total of subjects succes- through the regular pustular stage before scabbing.sively operated on was forty-three. Of these, six comprised In the second series of transfers, almost every case hadthe original transfer from the cattle direct, one from a goat, secondary eruptions, varying in number from 30 to 300, theseand the remaining thirty-six were successive transfers from generally appearing about the twelfth day, and having thethese subjects. Of the seven subjects originally operated on, same character as that just detailed, whilst the insertionsone absconded, in two cases the insertions showed the true themselves progress satisfactorily, like ordinary cases of vac-characters of vaccination, and in the remaining four, although cination. It will be seen in the experiments that the bullthe insertions progressed favourably, and in every respect re- operated on, on the 31st October showed indications of tume-sembled vaccination, small-pox eruptions showed themselves factions of the skin, and enlargement of the glands generally ;subsequently, and became scattered over their persons ; the and out of five animals, this was the only one in which thenumber of these pustules varying in the subjects from 30 to disease took this irregular form. I am inclined to attribute it200. Of these four subjects, the subsequent small-pox erup- to the excitement the animal was under, the distance it had totions made their appearance in three on the twelfth and in one travel over, crossing a river a mile wide, and having to swimon the ninth day after the original insertion. over several portions of it. These circumstances, perhaps,The insertions themselves ran their ordinary course, as is may account for the irregularity. At the same time, the off

usual in cases of vaccination. In every one of these cases the fore fetlock had a greasy appearance, and was moist withinsertions consisted of six punctures, three in each arm, and exudation ; and, irritated by flies, was covered with severalin the two subjects who had been free from secondary erup- bleeding points. This only showed itself with the tumefac-tions, two only in each case vivified, and in both these in- tions of the skin, and subsided with them. In the goat also,stances, on the seventh day after original operation, a fresh the insertion on the scrotum with small-pox virus showed areinsertion was made in the failed punctures from the same distinct umbilicated vesicle, resembling genuine vaccination,small-pox virus passed through the cow. but it was surrounded to the extent of two inches with a greasy

Of the reinsertions in these cases, in the first case, only one moist exudation, and peculiar musty odour. With this ex-

vivified, a small papule showing itself on the ninth day from ception, nothing else was to be found wrong with the animal.original, and third day after secondary, insertion, The papule In the cases where matter was transferred from the goat toformed a pustule on the eleventh day after first, and fifth day the human subject, although the insertions ran the usualafter second, operation; and the original insertions (the two satisfactory progress, on the twelfth day secondary eruptionsthat succeeded) had maturated, and were now forming scabs. to the extent of nearly 300 showed themselves. The ponyOn the 21st of November (or sixteen days after original, and having been lost sight of, results were not observed. Of

ten days after secondary, operation) the secondary pustule the two donkeys operated on, in one it failed altogether, and informed a small black spot, about the size of a large pin’s head, the other the insertions only vivified and ran their coursewhilst that of the original insertion, the scab of one had satisfactorily. In the dog also, the disease showed itself atdropped off, while the other was still adherent. the insertions only ; and when transferred to a second animal,On the 25th of November (or twenty days after original, the insertions only vivified and followed their usual course.

and fourteen days after secondary, operation) the two remain- Thus it will be seen that, in the lower animals, the insertioning scabs dropped off, the former leaving behind a true vaccine of small-pox virus takes on the ordinary character of vacci-cicatrix, and the latter a mere superficial minute scar on the nation, and is capable of being transferred either to otherarm. animals or to the human being.

In the second case the insertions were made on the seventh It will be seen in Case No. 2, Manuel, that on the seventhday after first operation, from genuine vaccine lymph; the day vaccine matter was inserted at the failed insertions, andtwo original insertions in this case had vivified, and were now it vivified and progressed in the manner described by Bryce,visible in the form of good vesicles. terminating with the originally-inserted virus; and, in anotherOn the 15th of November (or ten days after original, and instance, in a subsequent series of transfers, when, on the

four after secondary, operation) the original insertions had fourth day, there were no indications of the small-pox virusmaturated, and were scabbing, whilst the vaccine rcinsertions having passed through the cow vivifying in the subject, rein-had formed minute pimples containing a small quantity of pus. sertion of genuine vaccine lymph caused the original inser-The patient had vomited, and was suffering from nausea, high tions, as well as the secondary, to vivify and run their coursefever, pain in the axillea, and general malaise. satisfactorily.

16th. - Original and secondary eruptions progressing ; It will be observed from the above series of cases that whenvomited twice since last report. General constitutional symp- the inoculated small-pox matter from the cow was sent totoms are abating. Madras, where there was no small-pox epidemic prevalent at17th.-All the pustules have scabbed over, while the vaccine the time, genuine vaccine disease showed itself; but when the

scabs are very small; that from the small-pox virus resembles same virus was transferred to others at Arcot, where small-poxthe usual vaccine scab in size. had been for some time, and was then prevailing, vaccinia, or20th.-Scabs drying. a modified form of small-pox, showed itself between the ninth25th (twenty days after original, and fourteen after second- and twelfth days after inoculation, and only two subjects in-

ary operation).-The scabs of all the insertions have come away, oculated from small-pox matter direct from the cow were freeleaving good cicatrices on the original, and trifling scars on the from vaccinia. In all the other original and subsequent trans-secondary. fers at Arcot, vaccinia followed; and in one instance, where

It will be seen in both cases that the test known as four of the insertions of small-pox matter from the cow failed,"Bryce’s," which consisted in the reinsertion of virus as reinsertions of genuine vaccine matter, as late as the seventhlate as the seventh day, showed itself, and ran its course with day after primary operation, caused the original small-pox in-the original operation, both in the case of small-pox and sertions to vivify and run their regular course. This is an in-vaccine virus, teresting feature in this case, as the secondary insertions onlyLymph from these cases, charged in capillary tubes, and showed minute pimple-like formations, and the cicatrices were

forwarded to Madras,-’ succeeded well, and there was no also equally satisfactory.secondary eruptions ; whereas in a third case, operated on at Further, the view originally propounded by Jenner, thatthe same time and with the same virus as the above cases, on the cow-pox and grease in the horse were identical, is favoured by12th day after operation, some four or five papules were noticed the fact of grease showing itself in the fetlock of the first bullon the face. On the 19th there were from eight to ten small operated on, and in the scrotum of the goat, and appears toconical vesicles, containing a little limpid fluid. On the 2lst the me confirmatory of this view. However, further experimentswhole surface of the body was scattered over with the eruption, are necessary to clear the point in a more satisfactory manner.of which there must have been at least 50 or 60 in number. (To be concluded.)The eruptions which first showed themselves dried without _________________" ________________suppuration. A few vesicles on the hands and lower extremi-ties have the characteristic appearance of small-pox-viz., the ENERGETIC MEASURES BY LEARNED BODIES. - Forflat umbilicated look, and raised margin,-while some are hard some time past sulphate of quassina has been largely advertisedto the feel, many acuminated, and others forming little blad- in Naples, and used in several of the southern Italian provinces;der-like vesicles. The original insertions ill the arm have in- but Prof. de Luca, having doubts about the article, analysed it,creased in size, and are now scabbing. On the 28th, or and found that there is no such thing as quassina, or alkaloidtwenty-three days after operation, the scabs from inoculated of quassia. He submitted his opinion to the Medico-Chirurgicalinsertions have fallen a,wa.v. The secondary eruptions have Academy, who forthwith made application to the Council of

* Vide Table attacted. Health to stop the sale of this deceptive substance.