experimental design aas
DESCRIPTION
Experimental DesignGroup 4: Arceo, Ramos, SantosTitle: Analysis of Oil Spill Water Using AAS, UV-Vis, and FTIR.Sample: Oil spill water from Rosario, Cavite.Objectives:• To determine the concentration of heavy metals (Pb, Cd, Cr) in water sample using AAS.• To check for nitrates using UV-Vis spectrometer.• To determine the functional groups of present in the water sample using Fourier-Transform Infrared Spectrometer.TRANSCRIPT
AAS
[Cd] Vs Abs0.25 0
0.5 01.25 0
2.5 05 0
[Cr] Vs Abs0.25 0
0.5 01.25 0
2.5 05 0
[Pb] Vs Abs0.25 0
0.5 01.25 0
2.5 05 0
0 1 2 3 4 5 60
0.2
0.4
0.6
0.8
1
Cadmium [Cd]
Volume Standard
Abso
rban
ce
0 1 2 3 4 5 60
0.2
0.4
0.6
0.8
1
Lead [Pb]
Volume Standard
Abso
rban
ce
0 1 2 3 4 5 60
0.2
0.4
0.6
0.8
1
Lead [Pb]
Volume Standard
Abso
rban
ce
0 1 2 3 4 5 60
0.2
0.4
0.6
0.8
1
Chromium [Cr]
Volume Standard
Abso
rban
ce
AAS
[Cd] Trial 1 Cs Vs Vf CsVs/Vf Abs0.5 0 100 0 00.5 0 100 0 00.5 0 100 0 00.5 0 100 0 00.5 0 100 0 0
Blank
Trial 2 Cs Vs Vf CsVs/Vf Abs0.5 0 100 0 00.5 0 100 0 00.5 0 100 0 00.5 0 100 0 00.5 0 100 0 0
Blank
Trial 3 Cs Vs Vf CsVs/Vf Abs0.5 0 100 0 00.5 0 100 0 00.5 0 100 0 00.5 0 100 0 00.5 0 100 0 0
Blank
0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 10
0.5
1
Standard Addition for Cadmium (Trial 1)
CsVs/Vf
Abso
rban
ce
0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 10
0.5
1
Standard Addition for Cadmium (Trial 2)
CsVs/Vf
Abso
rban
ce
0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 10
0.5
1
Standard Addition for Cadmium (Trial 3)
CsVs/Vf
Abso
rban
ce
Equation of the lineMx 1
B 1
Equation of the lineMx 1
B 1
Equation of the lineMx 1
B 1
0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 10
0.5
1
Standard Addition for Cadmium (Trial 1)
CsVs/Vf
Abso
rban
ce
0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 10
0.5
1
Standard Addition for Cadmium (Trial 2)
CsVs/Vf
Abso
rban
ce
0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 10
0.5
1
Standard Addition for Cadmium (Trial 3)
CsVs/Vf
Abso
rban
ce
AAS
[Cr] Trial 1 Cs Vs Vf CsVs/Vf Abs0.5 0 100 0 00.5 0 100 0 00.5 0 100 0 00.5 0 100 0 00.5 0 100 0 0
Blank
Trial 2 Cs Vs Vf CsVs/Vf Abs0.5 0 100 0 00.5 0 100 0 00.5 0 100 0 00.5 0 100 0 00.5 0 100 0 0
Blank
Trial 3 Cs Vs Vf CsVs/Vf Abs0.5 0 100 0 00.5 0 100 0 00.5 0 100 0 00.5 0 100 0 00.5 0 100 0 0
Blank
0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 10
0.5
1
Standard Addition for Chromium (Trial 1)
CsVs/Vf
Abso
rban
ce
0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 10
0.5
1
Standard Addition for Chromium (Trial 2)
CsVs/Vf
Abso
rban
ce
0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 10
0.5
1
Standard Addition for Chromium (Trial 3)
CsVs/Vf
Abso
rban
ce
Equation of the lineMx 1
B 1
Equation of the lineMx 1
B 1
Equation of the lineMx 1
B 1
0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 10
0.5
1
Standard Addition for Chromium (Trial 1)
CsVs/Vf
Abso
rban
ce
0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 10
0.5
1
Standard Addition for Chromium (Trial 2)
CsVs/Vf
Abso
rban
ce
0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 10
0.5
1
Standard Addition for Chromium (Trial 3)
CsVs/Vf
Abso
rban
ce
AAS
[Pb] Trial 1 Cs Vs Vf CsVs/Vf Abs0.5 0 100 0 00.5 0 100 0 00.5 0 100 0 00.5 0 100 0 00.5 0 100 0 0
Blank
Trial 2 Cs Vs Vf CsVs/Vf Abs0.5 0 100 0 00.5 0 100 0 00.5 0 100 0 00.5 0 100 0 00.5 0 100 0 0
Blank
Trial 3 Cs Vs Vf CsVs/Vf Abs0.5 0 100 0 00.5 0 100 0 00.5 0 100 0 00.5 0 100 0 00.5 0 100 0 0
Blank
0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 10
0.5
1
Standard Addition for Lead (Trial 1)
CsVs/Vf
Abso
rban
ce
0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 10
0.5
1
Standard Addition for Lead (Trial 2)
CsVs/Vf
Abso
rban
ce
0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 10
0.5
1
Standard Addition for Lead (Trial 3)
CsVs/Vf
Abso
rban
ce
Equation of the lineMx 1
B 1
Equation of the lineMx 1
B 1
Equation of the lineMx 1
B 1
0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 10
0.5
1
Standard Addition for Lead (Trial 1)
CsVs/Vf
Abso
rban
ce
0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 10
0.5
1
Standard Addition for Lead (Trial 2)
CsVs/Vf
Abso
rban
ce
0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 10
0.5
1
Standard Addition for Lead (Trial 3)
CsVs/Vf
Abso
rban
ce
AASTrial 1 Trial 2 Trial 3
[Cd] Line Equation Line Equation Line Equation Concentrationy = y = y = y = Trial 1 1Mx 1 1 1 Trial 2 1B 1 1 1 Trial 3 1
Average 1Std. Dev 0
Trial 1 Trial 2 Trial 3[Cr] Line Equation Line Equation Line Equation Concentrationy = y = y = y = Trial 1 1Mx 1 1 1 Trial 2 1B 1 1 1 Trial 3 1
Average 1Std. Dev 0
Trial 1 Trial 2 Trial 3[Pb] Line Equation Line Equation Line Equation Concentrationy = y = y = y = Trial 1 1Mx 1 1 1 Trial 2 1B 1 1 1 Trial 3 1
Average 1Std. Dev 0
Concentration (Final)4.303 1.73 0
1 +/- 0
Concentration (Final)4.303 1.73 0
1 +/- 0
Concentration (Final)4.303 1.73 0
1 +/- 0
UVWavelength Absorbance Max Abs 1
200 1 Max Wavelength Ctrl F na lang210 1220 1230 1240 1250 1260 1270 1280 1290 1300 1310 1320 1330 1340 1350 1360 1370 1380 1390 1400 1410 1420 1430 1440 1450 1460 1470 1480 1490 1500 1510 1520 1530 1540 1550 1560 1570 1580 1590 1600 1
150 200 250 300 350 400 450 500 550 600 6500
0.2
0.4
0.6
0.8
1
1.2
UV/Vis
Wavelength
Abso
rban
ce
150 200 250 300 350 400 450 500 550 600 6500
0.2
0.4
0.6
0.8
1
1.2
UV/Vis
Wavelength
Abso
rban
ce
UV Max Wavelength ?Calibration
Standards Conc Abs1 0.5 0.0012 1 0.00113 3 0.00144 5 0.00155 10 0.0018
Blank ? ?
Input equation of the line: y = mx + b8E-05 0.001
Ibabaw (A) Concentration AbsTrial 1 16.25 0.0023Trial 2 136.25 0.0119Trial 3 25 0.003
Gitna ©Trial 1 75 0.007Trial 2 47.5 0.0048Trial 3 42.5 0.0044
Ilalim (b)Trial 1 67.5 0.0064Trial 2 26.25 0.0031Trial 3 3.75 0.0013
0 2 4 6 8 10 120
0.0005
0.001
0.0015
0.002f(x) = 8.07432432432433E-05 x + 0.00104510135135135R² = 0.93677840461821
Standard Calibration
Concentration
Abso
rban
ce
0 2 4 6 8 10 120
0.0005
0.001
0.0015
0.002f(x) = 8.07432432432433E-05 x + 0.00104510135135135R² = 0.93677840461821
Standard Calibration
Concentration
Abso
rban
ce