Experiment Results ReportVNN rt01Experiment SummaryExperiment Name:VNN rt01Experiment Type:Quantitation - Standard CurveFile Name:VNN rt01.edsRun Started:2014 Feb 18 1:10:06 AMRun Finished:2014 Feb 18 2:47:19 AMRun Duration:97 minutes 12 secondsDate Modified:2014 Feb 18 2:50:19 AMUser:jokoNumber of wells used:10Number of wells with results :10Instrument Name:Instrument Type: Applied Biosystems 7500 Fast InstrumentComments:User:joko1Printed:2014 Feb 18 4:43:52 AMExperiment:VNN rt01

Experiment Results Report

Applied Biosystems 7500 Fast InstrumentResults SummarySampleTargetQuantityQuantityCC

(Mean)(Std Dev)(Mean)(Std Dev)

1226VNN25.95

129VNN25.54

1926VNN30.66

224VNN24.40

45VNN34.35

46VNN35.77

47VNN33.30

522VNN31.64

723VNN39.97

827VNN36.66

22User:joko

Printed:2014 Feb 18 4:43:52 AMExperiment:VNN rt01

Experiment Results Report

Applied Biosystems 7500 Fast Instrument

33User:joko

Printed:2014 Feb 18 4:43:52 AMExperiment:VNN rt01

Experiment Results Report

Applied Biosystems 7500 Fast InstrumentAmplification Plot (Rn vs. Cycle)

44User:joko

Printed:2014 Feb 18 4:43:52 AMExperiment:VNN rt01

Experiment Results Report

Applied Biosystems 7500 Fast InstrumentAmplification Plot (Rn vs. Cycle)

55User:joko

Printed:2014 Feb 18 4:43:52 AMExperiment:VNN rt01

Experiment Results Report

Applied Biosystems 7500 Fast InstrumentAmplification Plot (C vs. Well)

66User:joko

Printed:2014 Feb 18 4:43:52 AMExperiment:VNN rt01

Experiment Results Report

Applied Biosystems 7500 Fast InstrumentStandard Curves

slope:null

Y-Intercept:null

R2:null77User:joko

Printed:2014 Feb 18 4:43:52 AMExperiment:VNN rt01

Experiment Results Report

Applied Biosystems 7500 Fast InstrumentResults TableWellSampleTargetTask QuantityC

A21926VNNUNKNOWN30.6598

A346VNNUNKNOWN35.7726

B2224VNNUNKNOWN24.4036

B347VNNUNKNOWN33.3025

C21226VNNUNKNOWN25.9505

D2827VNNUNKNOWN36.6559

E2129VNNUNKNOWN25.5368

F2522VNNUNKNOWN31.6422

G2723VNNUNKNOWN39.9675

H245VNNUNKNOWN34.3526

User:joko

8

Printed:2014 Feb 18 4:43:52 AMExperiment:VNN rt01

Experiment Results Report

Applied Biosystems 7500 Fast InstrumentQC SummaryTotal Wells96Processed Wells10Targets Used1

Well Setup10Flagged Wells0Samples Used10

Flag Name Frequency Locations

AMPNCAmplification in negative control0

BADROXBad passive reference signal0

BLFAILBaseline algorithm failed0

CTFAILC algorithm failed0

EXPFAILExponential algorithm failed0

HIGHSDHigh standard deviation in replicate group0

NOAMPNo amplification0

NOISENoise higher than others in plate0

NOSIGNALNo signal in well0

OFFSCALEFluorescence is offscale0

OUTLIERRGOutlier in replicate group0

SPIKENoise spikes0

THOLDFAILThresholding algorithm failed0

ZPRZero Passive Reference0

99User:joko

Printed:2014 Feb 18 4:43:52 AM