evaporative light scattering detectors - allpump.co.kr · 288 replacement parts for models 500,...
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Alltech ELSDI N S T R U M E N T S
Model 2000ES and Model 800 Evaporative Light Scattering Detector (ELSD)
Discover the Benefi ts of Sensitive, Universal HPLC Detection
ELSD has become the preferred method of detection for many diffi cult samples and is an indispensable tool for drug discovery researchers, complementing data from UV/Vis and MS. Alltech offers two ELSD models to address any performance or budget requirement. Choose from the advanced features of the ELSD 2000ES or the basic features and compact size of the Model 800. Either way you’ll experience the benefi ts of sensitive, universal evaporative light scattering detection.
For complete details of the Model 2000ES, see page 285-286.
For complete details of the Model 800, see page 287.
Detect these diffi cult samples:
Pharmaceuticals Nutraceuticals
Combinatorial Libraries Carbohydrates
Lipids Phospholipids
Triglycerides Fatty Acids
Polymers Surfactants
Amino Acids Impurities
Natural Products Peptides
Inorganic Ions
Model 800 ELSD • Economical – Priced thousands less than models with
comparable features• Easy to use – Requires virtually no optimization• Small and stackable – Makes effi cient use of valuable
bench space• Low temperature operation – Detects semi-volatile
compounds
Model 2000ES ELSD• The only ELSD with two operating modes – Get
optimum performance for all applications• Enhanced sensitivity – Even lower detection limits• Universal – Detect compounds missed by other
detectors• Advanced features – Increase productivity and
decrease downtime• Low temperature operation – Detect semi-volatile
compounds
Evaporative Light Scattering Detectors
The only ELSD with two operating modes The only ELSD with two operating modes
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464546464647
How Does It Work?
ELSDs detect any compound less volatile than the mobile phase using a simple three-step process:
Are There Any Limitations?
1. The mobile phase must be volatile.
2. The sample must be less volatile than the mobile phase.
3. Detection
The sample particles pass through a cell and scatter light from a laser beam. The scattered light is detected, generating a signal.
2. Evaporation
Droplets pass through a heated “drift tube“ where the mobile phase evaporates, leaving a fine mist of dried sample particles in solvent vapor.
1. Nebulization
Column effluent passes through a needle and mixes with nitrogen gas to form a dispersion of droplets.
Sensitivity
Gradient Capability
Baseline Stability
Solvent Interference
Mass Balance
Excellent:
Good:
Poor:
C H A R T K E Y E L S D R I U V M S
What Are the Advantages of ELSD Compared to Other LC detectors?
From the Leader in Evaporative Light Scattering Detection
Alltech has more than 15 years of experience engineering and manufacturing Evaporative Light Scattering Detectors. Our global network of dedicated application chemists and technical support professionals provide you with the comprehensive service and support you need. Let us show you how an Alltech ELSD can improve your analysis!
Alltech ELSDI N S T R U M E N T S
Evaporative Light Scattering Detectors
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Column: Alltima™ C18, 5µm, 150 x 4.6mm (Part No. 88302) Mobile Phase: A: Water B: Methanol Gradient: Time 0 8 10 20 25 %B 30 50 100 100 30 Flow Rate: 1.0mL/minColumn Temp: 40°C
10446
1. PEG 2. Clotrimazole
Column: Alltima™ HP EPS C18, 5µm, 150 x 4.6mm (Part No. 87715) Mobile Phase: 1% Acetic Acid:Methanol:Acetonitrile (70:20:10) Flow Rate: 1.0mL/min Column Temp: Ambient
1. Ephedrine 2. Caffeine
1. Aspartame 2. Hydrocortisone 3. Reserpine 4. Terfenadine
Column: ZORBAX® StableBond C18, 3.5µm, 50 x 4.6mm (Part No. 835975-902) Mobile Phase: A: 0.025% Formic Acid in Water B: 0.025% Formic Acid in Acetonitrile Gradient: Time: 0.0 1.0 5.0 5.1 %B: 25 70 70 25Flow Rate: 1.0mL/min Column Temp: 30°C
Chromatograms courtesy of Agilent Technologies
10449
10450
10458
10457
10456
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Alltech ELSDI N S T R U M E N T S
Use ELSD to Replace or Complement Data from Existing LC Detectors
Lotrimin® AF Topical Solution
1:1 Mixture
See What May Be Missing from Your UV/Vis Chromatogram
Obtain a More Accurate Representation of Sample Mass than UV/Vis
Use ELSD in Parallel with UV/Vis and MS for Maximum Structural and Concentration Information
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282 283
Column: Platinum™ C18, 3µm, 20 x 4.6mm (Part No. 43804)Mobile Phase: A: 0.05% Formic Acid in Water B: 0.05% Formic Acid in AcetonitrileGradient: Time: 0 3 7 10 %B: 5 90 90 5Flow Rate: 1.0mL/min
1. Aspartame 2. Cortisone 3. Reserpine 4. Dioctyl Phthalate
1. Gly 2. Ser/Asn 3. Asp 4. Gln 5. Ala/Thr 6. Glu 7. Cys/Lys 8. His
Column: Prevail™ C18, 5µm, 250 x 4.6mm (Part No. 99210) Mobile Phase: A: 5mM Heptafluorobutyric Acid, pH 1.0 w/0.7% TFA B: Acetonitrile Gradient: Time: 0 6 8 25 %B: 0 0 15 35Flow Rate: 1.0mL/min
Column: Prevail™ Carbohydrate ES, 5µm, 53 x 7mm Rocket™
(Part No. 35104) Mobile Phase: Acetonitrile:Water (75:25)Flow Rate: 2.0mL/min
10277
10278
1. Fructose 2. Glucose 3. Sucrose
10273
9. Pro 10. Arg 11. Val 12. Met 13. Tyr 14. Ile 15. Leu 16. Phe 17. Trp
9413
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Column: Adsorbosphere® C8, 5µm, 150 x 4.6mm (Part No. 287122)Mobile Phase: A: Water B: MethanolGradient: Time: 0 15 %B: 30 60Flow Rate: 1.0mL/min
10276
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Replace Refractive Index (RI) with ELSD for Higher Sensitivity and Stable Baselines
LC/MS Screening Performance Test Mix
Amino Acids, Underivatized
Use ELSD to Replace or Complement Data from Existing LC Detectors
Maintain Stable Baselines with Rapid, Extreme Gradients
Eliminate Derivatization of Amino Acids
Carbohydrates
Alltech ELSDI N S T R U M E N T S
PEG 600
Simplify Detection of Difficult Samples
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1. Glycerol
Column: Prevail™ Carbohydrate ES, 5µm, 250 x 4.6mm (Part No 35101)Mobile Phase: Acetonitrile: 0.04% Ammonium Hydroxide (80:20)Flow Rate: 1.0mL/min
Column: Alltima™ C18, 3µm, 150 x 1mm (Part No. 43864) Mobile Phase: Methanol:Water (90:10) Flow Rate: 50µL/min
1. n-Octyl Glucopyranoside (50ng on column) 2. n-Decyl Glucopyranoside (50ng on column) 3. n-Dodecyl Glucopyranoside (50ng on column)
Column: Alltima™ C18, 5µm, 150 x 10mm (Part No. 81102)Mobile Phase: Water:Acetonitrile (55:45)Flow Rate: 5.0mL/minColumn Temp: 25°C
1. Ethinamate (5.0 mg/mL)
10310
Column: Alltima™ C18, 3µm, 150 x 4.6mm (Part No. 81387)Mobile Phase: A: Dichloromethane B: AcetonitrileGradient: Time: 0 10 18 20 %B: 70 55 70 70Flow Rate: 1.5mL/min
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9528 10269
10270
10271
10272
10268 1. LLL 2. LLO 3. LLP 4. OOL 5. POL 6. OOO 7. OOP 8. PPO 9. OOS
10438
Alltech ELSDI N S T R U M E N T S
ELSD is a Versatile Detector for a Broad Range of Applications
Glucopyranosides
Use ELSD for Triggering Fraction Collection of UV/Vis Transparent Compounds in Preparative Applications
Use ELSD with Microbore Columns Without Modifying the Detector
Near Ambient Temperature Evaporation Maximizes Sensitivity for Semi-Volatiles
Detect Adulteration of Olive Oil Without Derivatizing the Sample
Triglycerides in Olive Oil
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12. DP4 13. DP5 14. DP6 15. DP7 16. DP8 17. DP9 18. DP10 19. DP11 20. DP12 21. DP13 22. DP14
10426
1. Itraconazole 2. Phosphatidyl- ethanolamine (PE) 3. Phosphatidylcholine (PC) 4. Lyso-Phosphatidyl- choline (Lyso-PC)
Column: Hypersil® Silica, 3µm, 150 x 4.6mm (Part No. 9855) Mobile Phase: A: Water B: Isopropanol C: Hexane Gradient: Time 0 7 13 14 16 %B 58 50 50 58 58 %C 40 40 40 40 40Flow Rate: 1.0mL/min
1. iso-Erythritol 2. Fructose 3. Sorbitol 4. Mannitol 5. Glucose 6. Inositol 7. Sucrose 8. Maltitol 9. Maltose 10. Raffinose 11. Maltotriose
Column: Prevail™ Carbohydrate ES, 5µm, 250 x 4.6mm (Part No. 35101)Mobile Phase: A: Acetonitrile B: 0.04% Ammonium Hydroxide in WaterGradient: Time: 0 25 40 60 %B: 17 27 45 55Flow Rate: 1.0mL/min
10009
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� 1. Glucosamine (1mg/mL)
Column: Prevail™ Carbohydrate ES, 5µm, 150 x 4.6mm (Part No. 35102)Mobile Phase: Acetonitrile:Water (65:35)Flow Rate: 1.0mL/min
10411
10413
10412
Column: Alltima™ C18, 5µm, 250 x 4.6mm (Part No. 88056)Mobile Phase: A: 0.05%TFA in Water:Methanol (95:5) B: 0.05%TFA in MethanolGradient: Time: 0 35 %B: 25 75Flow Rate: 1.0mL/min
10241
1. Bilobalide 2. Ginkgolide J 3. Ginkgolide C 4. Ginkgolide A 5. Ginkgolide B 6. Quercetin 7. Kaempferol 8. Isorhamnetin
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Column: Prevail™ Select C18, 5µm, 150 x 4.6mm (Part No. 99300) Mobile Phase: (95:5) Acetonitrile:Water pH 9 w/NH4OH Flow Rate: 1.0mL/min
1. Tylosin 2. Monensin
10319
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ELSD Simplifies Analysis of Phospholipids in a 1% Itraconazole Suspension
Mono-, Di-, Tri-Saccharides
Detect Non-Chromophoric Compounds Easily
ELSD Detects Glucosamine with Greater Sensitivity than UV or RI
Use Gradient Elution and Detect Non-Chromophoric Nutraceuticals
ELSD Simplifies Carbohydrate Analysis
Glucosamine
Ginkgo Biloba Standardized Extract
Alltech ELSDI N S T R U M E N T S
Monensin and Tylosin
ELSD Detects Non-Chromophoric Antibiotics without Derivatizing
Phospholipids in 1% Itraconazole Suspension
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10491
Column: Alltima™ C18, 5µm, 150 x 4.6mm (Part No. 88052)Mobile Phase: Methanol:Water (90:10)Flow Rate: 1.0mL/minColumn Temp: Ambient
1. n-dodecyl-β-D-glucopyranoside 1. Hydrocortisone (100ng on column)
6678
10464
Column: Alltima™ C18, 5µm, 4.6 x 250mm (Part No. 88056)Mobile Phase: Water:Acetonitrile (55:45)Flow Rate: 1.0mL/min
10463
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The ELSD 2000ES Increases the Limit of Detection (2.5ng on Column)
Alltech ELSDI N S T R U M E N T S
Detect compounds missed by other detectors with the near universal detection capabilities of the ELSD 2000ES
ELSD 2000ESNow the Most Advanced ELSD is Even More Sensitive!
• Now Even More Sensitive — lower detection limits• The Only ELSD with Two Operating Modes — get
optimum performance for all applications• Advanced Features — increase productivity and
decrease downtime• Low Temperature Operation — detect semi-volatile
compounds • Easy Integration into a Qualifi ed System — full
IQ/OQ/PQ protocols included with each ELSD
The ELSD 2000ES has all the benefi ts of the ELSD 2000 plus even greater sensitivity. The optical and electronic components have been redesigned to minimize background noise and increase sensitivity. The result of this new, patent pending technology is lower detection limits and greater signal-to-noise ratios.
For more information on the ELSD 2000ES, request brochure #497 or visit www.alltechWEB.com/ELSD.techinfo
The ELSD 2000ES Increases Sensitivity for Equal Concentrations of Hydrocortisone
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Column: Allsphere™ Silica, 3µm, 100 x 4.6mm (Part No. 778382)Mobile Phase: A: Isopropanol:Hexane:Water (58:40:2) B: Isopropanol:Hexane:Water (52:40:8)Gradient: Time: 0 7 19 20 %B: 0 100 100 0Flow Rate: 1.25mL/min
10214
Column: Prevail™ Carbohydrate ES, 5µm, 250 x 4.6mm (Part No. 35101) Mobile Phase: Acetonitrile:Water (85:15)Flow Rate: 1.0mL/min
10307
1. Urea (1µg on column)
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1. Phosphatidylethanolamine 2. Phosphatidylcholine
ELSD 2000ES
The Only ELSD with Two Operating Modes Gives You Optimum Performance with Any Application
Since an ELSD’s response is based on the amount of particles passing through the optical cell, it makes sense that sending 100% of the column effluent through would net the highest responses. In many cases this is true. Some samples, such as semi-volatiles may benefit from lower operating temperatures and gas flows achieved by splitting a portion of the aerosol stream to waste. Only the ELSD 2000ES offers the choice of splitting or not splitting, giving you optimum sensitivity for every application.
Splitting Allows Near Ambient Temperature Evaporation, Maximizing Sensitivity for Semi-Volatiles
Not Splitting is Best for Non-Volatile Samples and/or Organic Mobile Phases
Egg Yolk Phospholipid
Alltech ELSDI N S T R U M E N T S
The ELSD 2000ES has many features that enhance detector performance, increase productivity, and decrease downtime:
• Digital gas flow control ensures reproducible results• Automatic gas shut-off at the end of unattended runs conserves gas• Self-diagnostic tests automate troubleshooting and verify detector function at start up• Menu-driven graphical LCD and Windows-based PC control make programming easy• Method storage and recall simplify set-up• Alarm set points ensure tight control of critical operating parameters and trigger pump shutdown in case of error
Full IQ/OQ/PQ Protocols Included With Each ELSD
Alltech makes it easy for you to integrate the ELSD into a qualified HPLC system. The ELSD 2000ES is thoroughly tested before shipment and calibration and performance documents are included with each instrument. Detailed IQ/OQ/PQ procedures are included with the detector to help you perform your own qualification. We also provide the option of onsite qualification performed by an Alltech service engineer.
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6419KO
ELSD 800Experience the Benefi ts of Sensitive, Universal Evaporative Light Scattering Detection for the Price of a UV/VIS or RI Detector!
Low Cost, High Performance
The ELSD 800 is priced thousands less than other ELSDs with comparable features. Now everyone can afford to add universal HPLC detection to any HPLC system to replace or complement existing detectors.
Easy to Use
The ELSD 800 is easy to operate and requires virtually no optimization. The same low temperature and gas fl ow is used for most applications, including organic and aqueous mobile phases, and gradient methods.
Small and Stackable
The ELSD 800 is small and stackable, minimizing bench space needed, especially when using multiple ELSDs in close proximity.
Specifi cations ELSD 2000ES* ELSD 800Light Source: Laser diode with collimating optics, 650nm, 30mW
output, class IIIBLaser diode with collimating optics, 670nm, max output less than 5mW, class IIIA
Detector Element: Silicon photodiode Silicon photodiodeTemperature Range: Ambient to 120ºC in 1ºC increments Ambient to 110°C in 1°C incrementsNebulizer Gas: Nitrogen preferred; Calibrated to 4.0L/min Up to 3.0L/min, nitrogen recommendedInlet Pressure: 60-80psig 15–90psig (1.0–6.0 Bar)Typical Operating Range: 1.0-3.0L/min 1.0-3.0L/minFlow Control: Digital mass fl ow control Fixed orifi ce with regulated pressureMobile Phase Flow Rate: To 5.0mL/min To 5.0mL/minAnalog Output: 1V or 10mV full scale 1V or 10mV full scaleCommunications: Inputs: TTL/contact closure for auto zero and gas
shutoffOutputs: Contact closure for pump shutdown in error conditions
Outputs: Contact closure for pump shutdown in error conditions
User Interface: Graphical LCD with alphanumeric keypad and/or Windows® based PC control via serial I/O
Liquid crystal display and keypad entry of operating parameters
Power Requirements: 120/240V, 50/60Hz 120/240V, 50/60HzDimensions: 23.0" H x 12.5" W x 21.6" D
(58.4cm H x 31.8cm W x 54.8cm D)14.0" H x 13.0" W x 18.0" D(35.6cm H x 33.1cm W x 45.7cm D)
Weight: 35lbs (16Kg) 20lbs (9kgs)Warranty: 1 year parts and labor 1 year parts and laborIEC/FDA Classifi cation: Class 1 Laser Product n/a
EVAPORATIVE LIGHT SCATTERING DETECTORSDESCRIPTION PART NO. PRICE
ELSD 2000ES*120V 600100ES240V 600200ESELSD 800120V 800100240V 800200
The ELSD 800 and ELSD 2000ES have been designed and manufactured in accordance with the following standards: EN6101-1 + A2:95, EN555011: 1998, FCC CFR 47 Part 15: 2001, EN 61326: 1998, EN 6100-3-2: 1995 + A14:2000 and EN61000-3-3: 1995
Alltech ELSDI N S T R U M E N T S
*Patent Pending
*Patent Pending
Experience the Benefi ts of Sensitive, Experience the Benefi ts of Sensitive,
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REPLACEMENT PARTS FOR MODELS 500, 800, 2000, AND 2000ESDESCRIPTION PART NO. PRICE
NebulizersNebulizer for 800, 2000, and 2000ES ELSD 600514Kit for Replacing 500 Nebulizer with 800/2000 Nebulizer
500189
O-ringsKalrez O-ring for Nebulizer (800, 2000, and 2000ES)
600509
Viton O-ring for 500 Nebulizer, 5/pk MC0050White Variseal O-ring for 500 Nebulizer 500185Orange PTFE O-ring for Light Trap (all models)
600859
Small Orange PTFE O-ring for Optics Lens (all models)
600858
Large Orange PTFE O-ring for Light Cell (all models)
600860
LasersReplacement Laser for 800 and 2000 ELSD 600520Replacement Laser for 500 ELSD 500137Light Trap AssemblyFor Models 500, 800, and 2000 600800For Model 2000ES 600804Inlet Gas Filter (all models) 50135
Service and Support from a Leader in Chromatography
Our dedicated technical support professionals provide you with the comprehensive support you need. Our complete line of service products, including qualification, installation, training, repair service, and preventive maintenance, lets you choose what best meets your needs and budget requirements.
Alltech offers services, maintenance, and replacement parts for its current Models 2000ES and 800, and previous Models 2000 and 500. Service and replacement parts may also be available for the MKIII units. Contact Alltech for more information.
Qualification (IQ/OQ/PQ)
Alltech makes it easy for you to integrate the ELSD into a qualified HPLC system. Detailed IQ/OQ/PQ procedures are included with the ELSD 2000ES to help you perform your own qualification. We also provide the option of on-site qualification performed by an Alltech service engineer.
PREVENTIVE MAINTENANCEDESCRIPTION PART NO. PRICE
Preventive Maintenance Visit – Includes replacement of nebulizer, light trap, o-rings, and apertures
600365
Cleaning and NIST Calibration at Alltech500 ELSD 500361800 ELSD 8003612000/2000ES ELSD 600361Cleaning and NIST Calibration at Customer Site500 ELSD 500363800 ELSD 8003632000/2000ES ELSD 600363ELSD Service Agreement – 1-year repair, parts and labor at Alltech repair center, includes annual clean + NIST calibration
600370
ELSD Qualification, Service, and Replacement Parts
Installation and Training
Installation includes instrument set up and familiarization with operating principles and maintenance procedures. Additional training for user-specified topics is available at your site or at Alltech’s headquarters.
Preventive Maintenance and Repair
Alltech offers many flexible options for maintenance and repair of your ELSD. These services can be performed at your site or at Alltech. Service contracts are available. An annual cleaning and calibration is recommended. Extended warranties can be purchased to provide peace of mind and/or extend the warranty on refurbished instruments.
QUALIFICATION SERVICESDESCRIPTION PART NO. PRICE
2000ES ELSDIQ/OQ performed by Alltech Q60010ESPQ performed by Alltech Q60020ESIQ/OQ/PQ performed by Alltech Q60030ESRe-OQ performed by Alltech Q60040ESRe-OQ/PQ performed by Alltech Q60050ES2000 ELSDIQ/OQ performed by Alltech Q60010PQ performed by Alltech Q60020IQ/OQ/PQ performed by Alltech Q60030Re-OQ performed by Alltech Q60040Re-OQ/PQ performed by Alltech Q60050800 ELSDIQ/OQ performed by Alltech Q80010PQ performed by Alltech Q80020IQ/OQ/PQ performed by Alltech Q80030Re-OQ performed by Alltech Q80040Re-OQ/PQ performed by Alltech Q80050Qualification service is also available for the Model 500 ELSD. Contact your sales representative for details.
INSTALLATION AND TRAININGDESCRIPTION PART NO. PRICE
Installation and familiarization INSTALLTraining TRAINING
Alltech ELSDI N S T R U M E N T S
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6053
Alltech ELSDI N S T R U M E N T S
ELSD NITROGEN GENERATORDESCRIPTION PART NUMBER PRICE
ELSD Nitrogen Generator 600150Replacement Parts50 ft. 1/4" o.d. PTFE Tubing 31361/4" Brass Nuts, 10/pk 140591/4" Brass Ferrules, 10/pk 14062Plug Valve 121281/4"- 1/4" MPT Brass Male Connection 116391/8"-1/4" MPT Brass Male Connection 11635AccessoriesSingle-Stage Pressure Regulator, 0-200psig 81892MaintenanceMaintenance Kit 600151
• Simplifies ELSD operation• Produces a continuous supply of high purity nitrogen
from compressed air• Replaces inconvenient nitrogen cylinders• Requires no electricity
Water vapor and oxygen permeate through the wall of the membrane quickly. Only nitrogen flows through the fiber bores as the product stream.
SpecificationsNitrogen Purity: 99%Nitrogen Flow: 4L/minSuspended Liquids: NoneParticles: <0.01µmMin/Max Operating Pressure: 60/125psigDimension: 10.7" W x 13.4" D x 16.1" H
(27.2cm x 34.0cm x 40.9cm)Weight: 42.5lbsCompressed Air Requirements:Purity: Free of water, compressor oil (0.01mg/m3),
hydrocarbons, and particulates (<0.1µm) Temperature: 60°F (15°C) - 110°F (43°C)Compressor Outlet Pressure: 90-145psigCompressed Air Flow: 43L/min minimum
Increase productivity, reduce operating costs, and improve lab safety by eliminating the need to replace nitrogen cylinders. Forget about the need to change the nitrogen cylinder or wondering whether there is enough nitrogen left to finish the run. Take away the worry of flooding the ELSD and prevent costly repairs with a constant supply of nitrogen gas!
Alltech’s Nitrogen Generator requires no electricity and has no moving parts. Nitrogen is generated using a proprietary membrane separation technology. Simply connect the generator to your lab’s existing compressed air supply or suitable air compressor. The generator separates air into its component gases by passing compressed air through bundles of individual hollow-fiber, semipermeable membranes. As the air passes through these hollow fibers, water vapor and other “fast” gases such as oxygen and carbon dioxide permeate through the wall of the membrane quickly. Only nitrogen flows through the fiber bores as the product stream.
Alltech’s ELSD Nitrogen Generator produces a continuous, on demand supply of pure nitrogen at flow rates up to 4L/min. Two ELSDs may be used with one generator as long as your total gas flow rate is less than 4L/min.
The ELSD Nitrogen Generator has a small, compact footprint that fits easily on the lab bench next to your ELSD or mounts on the wall nearby.
Alltech ELSD Nitrogen Generator
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6433
UV DetectorsI N S T R U M E N T S
Add Affordable UV Detection to Any LC System
• Low maintenance• Upgrade as needed
The 460 Detector meets today’s needs with growth options for tomorrow. Flow cell and lamp options let you customize the detector for your application. Choose between 254nm and 280nm detection, and microbore, preparative, or standard HPLC sample cells. Save money by purchasing what you need today and upgrade with other options as your requirements change.
Detect most aromatic compounds with the standard 254nm lamp. Improve sensitivity for proteins, peptides, phenols, and catecholamines with the 280nm lamp. It’s easy to switch - just change the lamp and filter. Both lamps last for 4000-6000 hours and are self-aligning for easy replacement.
Alltech 460 UV Detector SpecificationsWavelength: 254nm or 280nmLamp: 254nm – mercury
280nm – phosphor coated mercury
Lamp Life: 4000 – 6000 hoursFlow Cells Standard Microbore Preparative Path Length: 10mm 2mm 1mm Volume: 19µL 1µL 15µLWavelength Accuracy:
±3nm
Wavelength Precision:
> ±0.2nm
Spectral Bandwidth:
0.1nm
Noise: < ±1 x 10-5AU Drift: < ±1 x 10-4AU Absorbance Range:
0.0005 – 4.0AU/V with voltage range of ±4V at any scale
Data Output: 1.0V/AUInputs: Autozero and event markDimensions: 6.6" H x 9.4" W x 15" DWeight: 12lb (5.5kg) Power: 100 to 240VAC at 50 to 60HzWarranty: 1 year
ALLTECH MODEL 460 UV DETECTORDESCRIPTION PART NO. PRICE
460 UV Detector with 254nm LampStandard Cell 460300Microbore Cell 460400Preparative Cell 460500460 UV Detector with 280nm LampStandard Cell 460600Microbore Cell 460700Preparative Cell 460800
REPLACEMENT PARTS AND ACCESSORIESDESCRIPTION PART NO. PRICE
Flow CellsStandard Cell 460301Microbore Cell 460401Preparative Cell 460501Replacement Lamp254nm 460200280nm 460201Filter for 280nm Configuration280nm Filter 460203
Note: For 280nm detection, you must change both the lamp and filter.
Alltech Model 460 Fixed Wavelength UV Detector
Affordable for today, adaptability for tomorrow.
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UV DetectorsI N S T R U M E N T S
LINEAR MODEL 200 UV/VIS DETECTORDESCRIPTION PART NO. PRICE
Model 200 DetectorWith 6mm, 9µL SS Flow cell110V 27410000220V 27410002With 6mm, 9µL Kel-F® Flow cell110V 27410010220V 27410020LampsDeuterium Lamp and Assembly 2037077Tungsten Lamp and Mounting Plate 2037079
LINEAR MODEL 205 UV/VIS DETECTORSDESCRIPTION PART NO. PRICE
Model 205 DetectorWith 6mm, 9µL SS Flow cell110V 205100220V 205200With 6mm, 9µL Kel-F® Flow cell110V 205110220V 205210LampsDeuterium Lamp and Assembly 2037077Tungsten Lamp and Assembly 2037078
For information on fl ow cell options requestinstrument support sheet #022. Also available on our website.
For information on fl ow cell options requestinstrument support sheet #023. Also available on our website.
Linear Model 200 Variable UV/Vis Detector
• Versatile, high performance detector• Simple analog controls• Flow cells for every
application• Pre-aligned lamps for
easy installation
The standard deuterium lamp covers UV wavelengths (190-365nm). An optional tungsten lamp is available for visible wavelengths (366-800nm). Flow cells are available to support a full range of applications from microbore to prep. The standard is a 6mm, 9µL 316 stainless steel cell for analytical applications. Kel-F® fl ow cells are available for biocompatible applications. Lamps and fl ow cells are easy to change and install.
Linear Model 205 Dual Wavelength UV/Vis Detector
• Increase sensitivity using wavelength programming
• Enhance detectability by monitoring two wavelengths simultaneously
• Flow cells for every application
The Model 205 comes with a deuterium lamp, and a stainless steel or Kel-F® 6mm, 9µL fl ow cell. Other fl ow cells are available to support a full range of applications from microbore to prep. Lamps and fl ow cells are easy to change and install. A cuvette holder converts the detector into a spectrophotometer.
Model 205 UV/Vis Specifi cationsWavelength Range: 190-365nm with deuterium lamp,
190-450nm or 366-700nm with dual wavelength, 366-800nm with tungsten lamp
Wavelength Accuracy: ±1.0nmWavelength Precision: ±1.0nmSpectral Bandwidth: 6nmNoise: < ±1.0 x 10-5AU @ 254nmDrift: < 2 x 10-4AU/hour @ 254nmAbsorbance Range: 0.0005-3.0AUFSCommunications: Remote inputs - Run, Stop, & Zero
Outputs - Ready & Accessory RelayPower: 100/120/220/240V; 50/60HzDimensions: 6.7" H x 11.8" W x 16.1" D
(17cm H x 30cm W x 41cm D)Weight: 30lbs (14kg)Warranty: 1 year, 90 days on fl owcell
Model 200 UV/Vis Specifi cationsWavelength Range: 190-800nm with deuterium and tungsten
lampsWavelength Accuracy: ±1.0nmWavelength Precision: ±1.0nmSpectral Bandwidth: 6nmNoise: < ±1.0 x 10-5AU @ 254nmDrift: < 2 x 10-4AU/Hour after warm-upAbsorbance Range: 0.0005-2.0AUFSDigital Display: 3-Digit LEDData Output: 1AU/VRecorder Outputs: 10mV, 100mV, 1V FSPower: 100/120/220/240V; 50/60HzDimensions: 6.3" H x 13.4" W x 9.8" D
(16cm H x 34cm W x 25cm D)Weight: 20lbs (9.1kg)Warranty: 1 year, 90 days on fl owcell
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2330
Fluorescence DetectorsI N S T R U M E N T S
Linear Model 305 Specifi cationsOptical Design: Dual monochromators using concave
holographic diffraction gratings; stepper motor driven
Sensitivity: S/N > 3000 for 4µg/L Anthracene in Methanol, 248nm Ex/398nm Em
Wavelength Range: 200-650nm and zero order excitation and emission; emission to 800nm with optional red-sensitive PMT
Wavelength Accuracy:
±2nm @ 248nm Ex/398nm Em
Spectral Bandwidth: 8, 20, or 30nm; user-selectable
Lamp: Pulsed xenon; selectable fl ash rate of 20 or 100 Hz
Flow cell: Hi-purity quartz, PTFE and PEEK; 8µL illuminated volume; maximum pressure 200psig (14 bar)
Spectral Scanning: Automatic or manual; 100 steps per second; step size selectable; 2, 4, 8, 16, or 32nm; up to 60 spectra stored in memory* replay or display stored data
Fluorescence Range: 0.01-500 fl uorescence units full scaleAnalog Outputs: 2, fully rangeable over entire fl uorescence
range using 20-bit D/AMethod Files: 4 user fi les protected in non-volatile
memory; fi les can be linked via queue; 10 time lines per fi le for wavelength changes; automatic zero
Ambient Environment:
10-40°C, 5-95% relative humidity, non-condensing
Communications: Remote Inputs: Run, stop, and zeroOutput: Ready
Display: 2x24 character, high contrast LCDDimensions: 6.7" H x 11.8" W x 15.7" D
(17cm H x 30cm W x 40cm D)Weight: 24.3lbs (11kg)Power: 100/120/220/240 VAC, 50/60 Hz
2 Amps maxWarranty: 1 year, 90 days on fl ow cell
*For scans of 100nm with a 2-nm interval (50 discrete data points).
LINEAR MODEL 305 FLUORESCENCE DETECTORDESCRIPTION PART NO. PRICE
Model 305 DetectorDual Monochromator, Programmable withPulsed Xenon Lamp, 2mm, 8µL Flow Cell
305100
AccessoriesXenon Lamp Assembly 304144Flowcell, Square 305204Standard Range PMT, 200-650nm 305027Extended Range PMT, 200-800nm 304032Excitation Lens with Holder 305206Emission Lens with Holder 305216Slit Wheel (8, 20, 30nm) 304395340nm Cut-Off Filter 304075Detector-to-Recorder Cable 304037
High Sensitivity Scanning Fluorescence Detection
• High-speed scan for both excitation and emission wavelengths
• LCD display of scan data• Pre-aligned lamp and fl ow cell
Dual monochromator design delivers exceptional optical performance and operation fl exibility for routine and trace analyses. Twenty-bit digital electronics enhance quantitative accuracy and precision, producing a wider dynamic range. The electronics design allows emission monitoring of fl uorescence, phosphorescence, and chemiluminescence.
Time programming of the Excitation wavelength and Emission wavelength lets you optimize for sensitivity and selectivity. Automatic, real time scanning of eluting peaks helps to identify optimum wavelengths.
The lamp and fl ow cell are easily accessible from the front of the instrument for quick exchange.
Need more information on the Fluorescence Detector? Request instrument support sheet #017. Also available on our website.
Linear Model 305 Fluorescence Detector
Need derivatizing reagents?Alltech carries derivatizing reagents that enhance UV and fl uorescence detection. See page 44 for details and ordering information.
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AutosamplersI N S T R U M E N T S
SPARE PARTSDESCRIPTION PART NO. PRICE
Pump Tubing Cassette 575115Side-Port Sample Needle, 3/pk 575130Large Volume Option, Field Installable 575165Air Needle 575125Sample Drain for Headspace Pressure 575170Auxilliary and Control I/O Cable 570101Tubing from Needle to Valve, 10ft 20064Needle Connection Nut 575175Needle Connection Ferrule, 10/pk 575185Standard Vial Tray 575110
ALLTECH MODEL 580 AUTOSAMPLERDESCRIPTION PART NO. PRICE
Model 580 AutosamplerStandard Tray, SS Valve 580100Standard Tray, PEEK Valve 580500Tray OptionsStandard Volume Tray, 84 Vial Capacity,1.5mL Vials with 3-10mL Vial Positions
580201
Large Volume Tray, 24 Vial Capacity,10mL Vials
580200
Large Capacity Tray, 96 Vial Capacity,1.5mL Vial
580265
Model 570 Autosampler• Affordably
automate HPLC and IC analysis
• Flushed loop fi lling maximizes precision and reproducibility
• Partial loop fi lling minimizes sample waste
ALLTECH MODEL 570 AUTOSAMPLERDESCRIPTION PART NO. PRICE
Flushed Loop FillingStainless Steel 575100PEEK 575500Partial Loop FillingStainless Steel 570150PEEK 570550
Model 580 Autosampler• Automates sample
prep tasks: sample dilution, derivatization, and internal standard addition
• Capable of three different injection techniques
• Large injection volume range formaximum versatility
• Built-in column heater
Model 570 Autosampler Specifi cationsVial Capacity: 96 samples (92 with needle wash), 48
(5mL) vials (optional)Injection Volume: Flushed Loop: 5-500µL (dispenser
controlled), 5-5000µL (by head space pressure).
Partial Loopfi ll: 10-490µL in steps of 10µL (depending on the loop volume, maximum 50% of the loop volume)
Injections/Vial: 9 MaximumNeedle Wash: Selectable, 50µL fi xed volume between vialsReproducibility: Flushed Loop Injections: RSD<=0.5%
Partial Loop Injections: RSD<=1.0%Power: 115 VAC/230 VAC; 50/60 HzDimensions: 11.8" W x 17.3" D x 11.1" H
(30cm W x 44cm D x 28cm H)Weight: 34lbs (15.5kg)Warranty: 1 year
Model 580 Autosampler Specifi cationsVial Capacity: 84 (1.5mL) vials + 3 (10mL) vials,
96 (1.5mL) vials (optional),24 (10mL) vials (optional)
Injection Volume: 1-5000µL in 1µL increments,5000-7500µL in 1.5µL increments,7500-10000µL in 4µL increments
Injections per Vial: 9 maximumAnalysis Time: 9hrs 59min 59sec maximumReproducibility: Full Loop Injections: <= 0.3% RSD,
Partial Loop Injections: <= 0.5% RSD,µL Pick-Up Injections(>10µL):<= 1.0%
Carry Over: <0.05% with needle washColumn Oven Temp. Range: Ambient +5° - 60°CTemperature Controlled Zone: 1" W x 1" D x 11.8" H
(2.5cm x 2.5cm x 30cm)Power Requirements: 115 VAC/230 VAC; 50/60HzDimensions: 11.8" W x 19.7" D x 13.4" H
(30 x 50 x 34cm)Weight: 33lbs (15kgs),
39lbs (18kgs) with cooling optionWarranty: 1 year
Multifunctional autosamplerimproves laboratory effi ciency.
Choose between fl ushed and partial loop fi lling and stainless steel and
PEEK fl uid paths.
Cooling and large volume options are also available. See instrument support sheet #027 for more information.
Cooling and large volume options are also available. See instrument support sheet #007 for more information.
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6772
HPLC PumpsI N S T R U M E N T S
Model 426 and 301HPLC PumpsLow Cost, High Performance Isocratic PumpsBoth pumps feature:
• Built-in pulse dampener and rapid refill for pulse-free operation
• Self-flushing pump head for longer seal-life• Automatic prime/purge system for easy priming• Wide flow range for broad HPLC work, including semi-
preparative applications
The Model 426 has these additional features:• User-settable upper and lower pressure limits• Pressure monitoring on the LCD• Interfacing features for convenient pump control
MODEL 426 AND 301 HPLC PUMP REPLACEMENT PARTSDESCRIPTION PART NO. PRICE
Pump Head Kit, PEEK 120800Pump Head Kit, Stainless Steel 120799Piston Seal Kit 120680Piston 121486Check Valve Kit, PEEK 060141Check Valve Kit, Stainless Steel 120679
Pump Comparison ChartMODEL CATALOG PAGE FLOW RANGE ISOCRATIC/GRADIENT PRESSURE MONITORING
301 HPLC Pump 294 0.01 - 10mL/min isocratic No 426 HPLC Pump 294 0.01 - 10mL/min isocratic Yes626 HPLC Pump 295 0.001 - 12mL/min isocratic or binary gradient Yes627 Preparative Pump 296 0.01 - 100mL/min isocratic or binary gradient Yes726 Quaternary Gradient Pump 297 0.001 - 5.000mL/min quaternary gradient Yes
Pump SelectionWe Have a Pump for Every Need and Budget!
Use this pump feature comparison chart to choose the pump that’s right for your application, then go to the catalog page for more information.
Model 426 & Model 301 HPLC Pump SpecificationsMax. Operating Pressure: 6000psig (SS), 5000psig (PEEK)
Flow Rate Range: 0.01 to 10mL/min
Flow Rate Precision: 0.2% RSD
Flow Accuracy: ±2.0%
Dimensions: 6" H x 11" W x 18" D
(15cm x 26cm x 46cm)
Weight: 30 lbs (14 kg)
Warranty: 1 year parts,excluding seals and check valves; 1 year labor
MODEL 426 AND 301 HPLC PUMPSDESCRIPTION PART NO. PRICE
Model 426 HPLC PumpPEEK, 85-265VAC, 47-63Hz 426150Stainless Steel, 85-265VAC, 47-63Hz 426250Model 301 HPLC PumpPEEK, 85-265VAC, 47-63Hz 301150Stainless Steel, 85-265VAC, 47-63Hz 301250
Both pumps are available in stainless steel or biocompatible PEEK
Need Tubing?See our tubing section p. 230-235 for a complete line of low and high pressure HPLC tubing.
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Filter Funnel
Base
6773
HPLC PumpsI N S T R U M E N T S
Model 626 HPLC PumpHigh Performance Isocratic or Binary Gradient Pump
• Suitable for standard and microflow applications• Dual piston mechanism for pulse-free operation• Built-in low dead volume pulse dampener
• Self-flushing pump head for longer seal life
The Model 626 Pump is a rugged and reliable component for a basic analytical system, a sophisticated method development system, or for general laboratory or industrial use. It is available in either traditional 316 stainless steel or biocompatible PEEK.
Buy a single 626 Pump for isocratic applications. Add a second 626 pump for high-pressure binary gradients.
ACCESSORIES AND REPLACEMENT PARTSDESCRIPTION PART NO. PRICE
AccessoriesHigh-pressure Gradient Kit, PEEK(Includes controller, mixing tee, fittings and tubing)
105626
High-pressure Gradient Kit, Stainless Steel(Includes controller, mixing tee, fittings and tubing)
105624
Replacement PartsPiston Seal Kit 160566Piston 160565Check Valve Kit, Stainless Steel 120679Check Valve Kit, PEEK 060141
Model 626 HPLC Pump SpecificationsMax. Operating Pressure: 6,000psig (Stainless Steel)
5,000psig (PEEK)Flow Rate Range: 0.001 - 12.0mL/min Flow Rate Precision: 0.2% RSD Flow Accuracy: ±2.0%Gradient Mixing Mode: High-Pressure Gradient Profiles: Programmable Multi-stepGradient Programming: 10 methods with up to 100 lines per
method
Dimensions: 6" H x 11" W x 18" D(15cm x 26cm x 46cm)
Weight: 30 lbs (14 kg)Warranty: 1 year parts, 1 year labor, excluding
seals and check valves
Note: All replacement parts kits contain parts for one pump head only.
POLYPROPYLENE FILTER FUNNEL SYSTEMDESCRIPTION PART NO. PRICE
1L Filter Funnel SystemIncludes: base, adapter kit, mesh filter support, 1L funnel
6409
2L Filter Funnel SystemIncludes: base, adapter kit, mesh filter support, 2L funnel
6429
MODEL 626 HPLC PUMPDESCRIPTION PART NO. PRICE
Isocratic SystemPEEK, 85-265VAC, 47-63Hz 626150Stainless Steel, 85-265VAC, 47-63Hz 626250Binary Gradient System(Includes 2 pumps, system controller, mixing tee, tubing and fittings)
PEEK, 85-265VAC, 47-63Hz 626350Stainless Steel, 85-265VAC, 47-63Hz 626450
Use one pump for isocratic applications, or two pumps for high-pressure binary gradient applications.
Polypropylene Filter Funnel SystemsUnbreakable, inert polypropylene.Leak-proof GL45 threads.Use with 47mm filter membranes.In-line version available - visit our website.
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HPLC PumpsI N S T R U M E N T S
Ideal for Scale-up Work, Semi-Prep and Preparative HPLC
• Dual piston mechanism for pulse-free operation• Built-in low dead volume pulse dampener• Self-flushing pump head for longer seal life• Isocratic or high-pressure binary gradient
The Model 627 Preparative Pump delivers the accuracy and precision of a standard analytical pump at flow rates up to 100mL/min. Consistent flow rates and pressures minimize the potential for column damage. The 627 pump is available in 316 stainless steel or biocompatible PEEK.
Buy one pump now and upgrade to a binary system later by purchasing a second pump and a high-pressure gradient kit.
KITS AND ACCESSORIESDESCRIPTION PART NO. PRICE
AccessoryHigh-pressure Gradient Kit, PEEK(Includes controller, mixing tee, fittings and tubing)
105625
High-pressure Gradient Kit, SS(Includes controller, mixing tee, fittings and tubing)
105628
Replacement PartsPiston Seal Kit 120681Piston 121504Check Valve Kit, Stainless steel 120679Check Valve Kit, Metal-Free 060141
Note: All replacement parts kits contain parts for one pump head only.
Model 627 HPLC Pump SpecificationsMax. Operating Pressure: 4,000psig (both SS and PEEK)Flow Rate Range: 0.1- 100.0 mL/min Flow Rate Precision: 0.5% RSDFlow Accuracy: ±2.0%Gradient Mixing Mode: High-Pressure Gradient Profiles: Programmable Multi-stepGradient Programming: 10 methods with up to 100 lines
per method
Dimensions: 6" H x 11" W x 18" D(15cm x 26cm x 46cm)
Weight: 35 lbs (16 kg)Warranty: 1 year parts, 1 year labor,
excluding seals and check valves
MODEL 627 PREPARATIVE PUMPDESCRIPTION PART NO. PRICE
Isocratic SystemPEEK, 85-265VAC, 47-63Hz 627150Stainless Steel, 85-265VAC, 47-63Hz 627250Binary Gradient System(Includes 2 pumps, system controller, mixing tee,tubing and fittings)
PEEK, 85-265VAC, 47-63Hz 627350Stainless Steel, 85-265VAC, 47-63Hz 627450
Model 627 Preparative HPLC Pump
Flow rate range of 0.1mL/min to 100.0mL/min.
Need a Prep Column?Alltech manufactures preparative columns packed with popular Alltech packing materials in 7-,10-, and 22mm i.d. formats. Larger i.d. columns are available upon request. Call 1-800-255-8324 for help with choosing the right prep column for your application.
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800
600
400
200
0
0 10 20 30 40 50 Min.
Volta
ge [m
V]
Mobile Phase: A: Acetonitrile B: Acetonitrile with Methyl ParabenGradient: Time: 0 6 20 25 25.01 30 30.01 35 35.01 40 40.01 45 45.01 50 B%: 0 0 100 100 80 80 60 60 40 40 20 20 0 0 Flow Rate: 1.0mL/minDetector: UV @ 254nm
HPLC PumpsI N S T R U M E N T S
High-pressure Quaternary Gradients for Standard or Micro Applications
• Integrated pulse dampener reduces pulsations and mixes gradients accurately
• Self-flushing pump heads extend seal life• Easy gradient programming with hand held control
module
The Model 726 Quaternary Gradient Pump combines four high performance pumps in a single cabinet. The system controller controls each pump independently, for accurate gradients. Flow rates are programmable from 0.001mL/min to 5.000mL/min making it suitable for standard or micro applications. The 726 pump is available in 316 stainless steel or biocompatible PEEK. TTL contacts are available for remote Run and Stop inputs.
Model 726 Quaternary Gradient Pump SpecificationsMax. Operating Pressure: 6,000psig (Stainless Steel)
5,000psig (PEEK)
Flow Rate Range: 0.001 - 5.000mL/minFlow Precision: 0.2% RSD Flow Accuracy: ±2.0%Gradient Mixing Mode: High-Pressure GradientProfiles: Programmable Multi-stepGradient Programming: 0-100% in 0.1% incrementsDimensions: 6.75"H x 14.0"W x 16.0"D
(17cm H x 36cm W x 41cm D)
Weight: 50 lbs (23 kg)Warranty: 1 year parts, excluding seals and
check valves; 1 year labor
Overlay of same gradient profile run with 5 different pump head combinations
ALLTECH MODEL 726 QUATERNARY GRADIENT HPLC PUMPDESCRIPTION PART NO. PRICE
Stainless Steel110V 726100220V 726101PEEK110V 726200220V 726201Replacement PartsPiston Seal Kit 160566Piston Assembly 160565Check Valve Kit, Stainless steel 120679Check Valve Kit, PEEK 060141
Note: All replacement parts kits contain parts for one pump head only.
Model 726 Quaternary Gradient HPLC Pump
The 726 quaternary gradient pump
delivers accurate and reproducible
solvent proportioning.
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6219
Column HeatersI N S T R U M E N T S
MODEL 631 COLUMN HEATERDESCRIPTION PART NO. PRICE
Model 631 Column Heater, PEEK110V 631100220V 631200Model 631 Column Heater, Stainless Steel110V 631300220V 631400
Alltech Model 631 Column Heater Specifi cationsColumn ChamberCavity Dimensions: 1.8" H x 5.0" W x 16.5" D
(5cm x 13cm x 42cm)PreheaterMetal Free Version: 2' x 0.01" i.d. PEEK coil cast in
tin alloy blockSS Version: 2' x 0.01" i.d. SS coil cast in tin
alloy blockTemperature ControlTemperature Control: Ambient to 85°CTemperature Accuracy: ±1°CTemperature Stability: ±0.10°CPhysicalOperating Voltage: 110V, 220V Outside Case Dimensions: 5.0" H x 10.3" W x 19.5" D
(13cm x 26cm x 50cm)Warranty: 1 year
Unique drawer provides fast, convenient access to columns and conserves bench space.
• Microprocessor controlled for excellent temperature precision (±0.10°C)
• Column preheater improves performance through enhanced thermal stability
A large column chamber accepts two columns up to 30cm long and 1⁄2" o.d. plus guard columns. The chamber is easily accessed without removing the instrument cover. Bench space requirements are reduced because other instruments can be stacked on top of the heater. A mounting bracket is provided to install an injection or switching valve on the side panel. Available in stainless steel or PEEK fl ow paths.
Column Heater Selection GuideUse this feature comparison chart to choose the column heater that’s right for your application, then go to the
catalog page for more information.
Column Heater Selection Guide
MODEL ACCESS TO COLUMNS TEMPERATURE RANGECOLUMN CAPACITY
(EXCLUDING GUARDS)MAXIMUM COLUMN
O.D.MAXIMUM COLUMN
LENGTH CATALOG PAGE
Alltech 631 drawer Ambient - 85°C 2 1/2" 30cm 298Eppendorf CH-30 cover Ambient - 150°C 1 1/2" 30cm 299Eppendorf CH-430 cover Ambient - 150°C 4 1/2" 30cm 299Eppendorf CH-460 cover Ambient - 150°C 4 1/2" 50cm 299
Jetstream Plus/Plus 2 door 5°C - 85°C 5 1" 35cm 300Water Jacket endfi ttings Ambient - 90°C 1 1/2" 100cm 300
Alltech Model 631 Column Heater
PRICE
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Column HeatersI N S T R U M E N T S
Eppendorf™ TC-50 Temperature Controller SpecificationsSet/Read Display: 4 digit LED with Set/Read indicatorsTemperature Adjust: 0.1°C increments via 10-turn
potentiometerRange: Ambient to 150°CStability: ± 0.1°C typical core stabilityAbsolute Accuracy: ± 1°C at 50°CDimensions: 3-1/4" H x 7" W x 8-1/2" D
(8.3cm x 17.8cm x 21.6cm)Warranty: 1 year
EPPENDORF™ CONTROLLERS AND COLUMN HEATERSDESCRIPTION PART NO. PRICE
Temperature ControllersTC-50, 120V 85910TC-50, 240V 85911Column Heaters*CH-30, 120V 85975CH-30, 240V 85976CH-430, 120V 85980CH-430, 240V 85981CH-460, 120V 85971CH-460, 240V 85972AccessoriesTwo Column Adapter for CH-30,2/pk 85168Column Envelope, 1/4 x 7-1/2" 185120Column Envelope, 3/8 x 7-1/2" 185121Column Envelope, 1/2 x 7-1/2" 185122PEEK Tee with Fittings 32143
*TC-50 Temperature Controller required.
Eppendorf™ CH-430 & CH-460 Column Heater SpecificationsColumn Size: 4 columns up to 1/2" (1.3cm) o.d., all
lengths to 30cm plus fittings (CH-430) or 50cm plus fittings (CH-460)
Inlet Preheaters: Four sets includedHeating Element: 300W (CH-430) or 500W (CH-460) vulcanized
to heating blockHeater Block: Aluminum, two-part ovenSensor: 1000Ω Platinum RTDThermal Cut-Out: 155°C ± 5°C temperature limitDimensions: CH-430: 3-1/2" H x 6-1/2" W x 21" D
(8.9cm H x 16.5cm W x 53cm D)CH-460: 3-1/2"H x 6-1/2" W x 29-1/2" D(8.9cm H x 16.5cm W x 74.9cm D)
Warranty: 1 year
Eppendorf™ CH-30 Column Heater SpecificationsColumn Size: Any o.d. up to 1/2", all lengths to 30cm plus
fittingsInlet Preheaters: IncludedHeater Element: 125W vulcanized to heating blockHeater Block: Aluminum, two-part oven Sensor: 1000Ω Platinum RTDThermal Cut-Out: 155°C ± 5°C temperature limitDimensions: 3" H x 2-3/4" W x 19-1/2" D
(7.6cm x 7cm x 49.5cm)Warranty: 1 year
Couple the TC-50 Temperature Controller with the heater that best suits the column dimensions and specifications for your application. All are made of sturdy 1/8" thick aluminum parts for years of reliable service in the lab environment.
Model TC-50 Temperature Controller
• Controls any Eppendorf™ heater from ambient to 150°C
• Monitor “set” and “read” on the LED• Remote feature allows external ON or OFF
CH-430 and CH-460 Column Heaters*
Accommodates four columns up to 1/2" o.d. and two Rheodyne valves in the heated zone.
CH-30 Column Heater*
Fits one column up to 1/2" o.d. and 30cm in length.
• CH-430 holds columns up to 30cm in length• CH-460 holds columns up to 50cm in length• Ideal for multi-column GPC and applications requiring
sample injection or column switching in the heated zoneAdjust temperature in 0.1°C increments
Eppendorf™ Column Heaters
• Moveable PTFE V-supports can support column lengths up to 30cm
*TC-50 Temperature Controller required.
*TC-50 Temperature Controller required.
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Column HeatersI N S T R U M E N T S
The wide temperature range and large heated zone make the Jetstream Plus and Jetstream Plus 2 ideally suited for a wide range of HPLC applications. The column temperature is controlled using two-way forced air circulation for fast heating and cooling. A controller with a numerical keypad and a two-line LCD is used for programming.
The units have features for safe, reliable operation: self-check and auto calibration at power-on, leak detection, and automatic over temperature switch off.
• Easy to connect and use• Low cost alternative to column heaters
Alltech’s Water Jacket is an effective and easy way to control the temperature of HPLC columns. Maximum allowable temperature is 90°C. Several sizes are available for jacketing columns up to 100cm long and up to 1/2" o.d.. Connections to the circulating bath are made with 1/4" o.d. flexible tubing. The water jacket is made of clear acrylic tubing, 1.5" o.d. x 1" i.d..
The end connections are designed to form a tight seal around 1/16" o.d. tubing. Connecting tubing is supplied with each water jacket. The entire column, including endfittings, is contained within the water jacket. One year warranty.
1/16" o.d. Outlet Tubing to Detector
1/16" o.d. Inlet Tubing from Injector 1/4" o.d.
Water Inlet Line
Clear Water Jacket
Column
1/4" o.d. Water Outlet
JETSTREAM PLUS & JETSTREAM 2 PLUS COLUMN HEATERS/COOLERSDESCRIPTION PART NO. PRICE
Jetstream Plus Column Heater/Cooler100-245V, 75cm Cable 531101100-245V, 150cm Cable 531201Jetstream Plus 2 Column Heater/Cooler100-245V, 75cm Cable 531102100-245V, 150cm Cable 531202
Jetstream Plus & Plus 2 SpecificationsTemperature Range: 5-85°CTemperature Accuracy: ±0.2°CTemperature Stability: ±0.1°CTemperature Change: 2°C/minHeated Zone Dimension*: 39.2cm H x 8.5cm W x 5cm LDimension: 45cm H x 13.5cm W x 31cm LWeight: 8 kgPower: 100-245VAC, 50/60 Hz, 100WWarranty: 1 year
*Additional space above the heating/cooling element is available for injection and switching valves.
• Peltier column thermostat heats to 85°C and cools to 5°C
• Temperature may be fixed or changed via step or linear gradient
• Large column compartment holds five columns up to 1" o.d. and 35cm in length
Can be installed vertically or horizontally.
ALLTECH WATER JACKETDESCRIPTION PART NO. PRICE
Column Water Jacketsfor 5 to 10cm Columns 95023for 10 to 25cm Columns 95024for 25 to 30cm Columns 9502for 30 to 50cm Columns 95021for 50 to 100cm Columns 95022
Jetstream Plus and Plus 2 Column Heater/Cooler
Alltech Column Water Jackets
What’s the difference between Jetstream Plus and Plus 2?In the Jetstream Plus, the tubing to and from the column is fed between the sealing gasket of the door and the main unit. In the Jetstream Plus 2, several capped openings are provided on the sidewall of the unit to make the tubing connections.
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Data CollectionI N S T R U M E N T S
PE Interface Control • Preserve your investment in existing PE Interface
hardware• Take advantage of advances in computer and software
development
EZStart™ 3.1 with PE Interface Control License allows PerkinElmer Inc’s interface boxes to be used as analog to digital converters to interface chromatography detectors to EZStart™. Control the PE Turbochrom, Turbochrom EL, TotalChrom, and ACCESS*CHROM interfaces from EZStart™ to take advantage of the software benefi ts while retaining the investment in hardware.
PE Interface Control Software controls the following Perkin Elmer Interfaces:
- Series 900 Model 941/941A (RS232 communications)- Series 900 Models 950/950A, 960/960A, 970/970A
(GPIB communications)- NCI 901/902 (GPIB and RS232 communications)
Ordering Information
GPIB PE Interfaces require a GPIB interface card for EZStart™ (Part No. 111420, PCI slotted card). Serial PE Interfaces require available RS232 ports.
Affordable, Simple-to-use Chromatography Data System
• PC-based data handling at integrator-like prices• Optional control of instruments from many vendors• Upgradeable to EZChrom Elite™
Based on the EZChrom Elite™ software package, EZStart™ is the ideal solution for laboratories wanting a simple-to-use chromatography data system with comprehensive software features and optional instrument control. It is intended to provide the basic requirements for data acquisition and instrument control from a single workstation.
Operation is reduced to three easy steps:
1. Simple confi guration. Collects data from any instrument generating an analog output using the software’s SS420X data interface.
2. Run in one window. Set up all runs from a single window using simple wizards.
3. Generate graphic reports. Select from existing templates or customize with the report editor.
For more information on EZStart™ Software, including instrument control options, request sheet M078. Also available on our website.
For more information on EZStart™ PE Interface Control Software, request sheet M098. Also available on our website.
EZSTART™ SOFTWARE WITH SS420X INTERFACE DESCRIPTION PART NO. PRICE
120V 1886011220V 1886211100V (Japan) 1886311
EZStart™ Chromatography Data System
EZSTART™ CHROMATOGRAPHY DATA SYSTEMDESCRIPTION PART NO. PRICE
EZStart 3.1 with PE Interface Control* 1886023IEEE-488 PCI Card (for PE-Nelson 700 and 900 series intelligent interfaces)**
111420
*One PE interface is required but not included**Available PCI slot in PC required
Computer Requirements: - Windows XP (SP1), Windows 2000 (SP3), or
Windows NT (SP6)- Microsoft Internet Explorer version 4.0 or
greater
Some EZStart™ packages require, but do not include, a GPIB card from Alltech and Scientifi c Software. RS232 ports on the computer may be required for some instruments.
Recommended System:- Pentium IV (800MHZ), 128 MB RAM, 10 GB
hard disk space
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Data CollectionI N S T R U M E N T S
IntegratorsThermo Electron ChromJet™ Integrators
THERMO ELECTRON CHROMJET™ INTEGRATORDESCRIPTION PART NO. PRICE
Single ChannelUSA Warranty* 50303000European Warranty** 50303001Two ChannelUSA Warranty* 50303010European Warranty** 50303011*Integrators must be returned to Thermo Electron USA Office for service.**Integrators must be returned to Thermo Electron European Office for service.
Thermo Electron ChromJet™ SpecificationsINPUT CHARACTERISTICS
Analog-to-Digital Conversion: Voltage-to-frequency typeInput Range: -10mV to +1.024VSampling Rate: 50 or 60 points/secArea Per Count: 0.5µV-sec/area countInput Impedance: 1,000 MΩ, minimumDynamic Range: 106Linearity: ±(0.1% of signal + 0.005% full
scale)INKJET PRINTER/PLOTTER
Chart Speeds: 11 speeds from 0.1-20cm/secAttenuation: 14 levels from 0.5-4, 096mV full
scaleDisplay: 4 line, 40 character LCD
MEMORY AND PEAK STORAGE
System Memory: 24K of RAMStorage Memory: 488K of RAMTotal Memory: 512K of RAMMemory Expansion: 384K of RAMComputer Interface: RS-232C 110 to 9600 baud
(4800, 9600 for output only)Power: 110-240V (automatically selected)
PHYSICAL
Dimensions: 7" H x 15" W x 18" D(17.8 x 38.1 x 45.7cm)
Weight: 14.8lb (6.71kg)Warranty: 1 year
ACCESSORIESDESCRIPTION PART NO. PRICE
Startup Kit (Incl. Paper Carrier, 2500 Sheets Z-Fold Paper, 5 Inkjet Cartridges
50303020
Z-Fold Paper, 2500 Sheets/Box 50303030Inkjet Cartridges, 5/pk 50303040Extended Basic for ChromJet 50303101Remote Start Cable 50304040External Events Cable 50305050
MEMORY EXPANSION, 384K DESCRIPTION PART NO. PRICE
Serial Number <10,000 50303105Serial Number >10,000 50303107
HEWLETT-PACKARD INTEGRATOR ACCESSORIESDESCRIPTION PART NO. PRICE
General Purpose Analog Signal Cable to Connect to Non-HP Equipment (with Spade Lug Terminators)
15489
General Purpose Remote Control Cable 15408Z-Fold Paper, 5pkg/box 15609Inkjet Paper, 4 rolls/box 15612Ink Cartridge, ea 15611
A remote start cable (Part No. 50304040) is included with each integrator.
• Baselines drawn on original chromatograms • Automatic re-plot and reintegration• Multi-level calibration, linear, or non-linear fitting• Automatic calculation of area %, height %, internal
standard, external standard, and normalization %
For over 20 years chromatographers have used and depended on Thermo Electon integrators for accurate, reliable integration and reporting. The ChromJet™ integrator is a full-feature, high performance integrator. It has a full-size PC-style keyboard and LCD display for easy setpoint entry and editing.
The ChromJet™ can store and reprocess up to 128 chromatograms individually or in batches. Up to 16 methods can be stored and used to control integration parameters. An RS-232C port interfaces the integrator to computers and external storage devices. A battery backs up data for up to 6 months.
Accessories for Hewlett-Packard Integrator
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Data CollectionI N S T R U M E N T S
Chart RecordersKipp & Zonen BD 11/11E & BD 12/12E Chart Recorder
• High sensitivity – up to 400V full scale• Home and grid functions• User selectable left or right zero positions
KIPP & ZONEN BD 11/11E 12/12E CHART RECORDERSDESCRIPTION QTY. PART NO. PRICE
BD 11 Single Channel, 110/220V ea 110200BD 11E Single Channel with Electric Pen Lift and Remote Control,110/220V
ea 110201
BD 12 Dual Channel, 110/220V ea 120200BD 12E Dual Channel with Electric Pen Lift and Remote Control,110/220V
ea 120201
Models BD 11/11E & BD 12/12E Recorder Specifi cationsChart Speeds: 0.1, 0.2, 0.5, 1, 2, 5, 10, 20mm/min or
mm/secInput Ranges: 1, 2, 5, 10, 20, 50, 100, 200, 500mV and 1,
2, 5, 10, 20V Full ScaleInput Impedance: 1M DCZero Adjust: -50 to +150% Full ScaleNon Linearity: <0.3% Full Scale Defl ection (200mm)Response Time: <0.2sec (5-95% Full Scale Defl ection)Power: 110/220V, 50/60Hz,Dimensions: 3.5" H x 15" W x 11.4" D
(9.0cm H x 38.0cm W x 29.0cm D)Weight: 7.7lbs (3.5kg)Other Features: Meets CSA and VDE Specifi cationsWarranty: 1 Year, Parts and Labor
KIPP & ZONEN ACCESSORIESDESCRIPTION QTY. PART NO. PRICE
Chart PaperLinear Graduation Paper 10 40408Disposable Replacement PensRed 6 40410Blue 6 40412Green 6 40414Black 6 40416Red 2nd Channel 6 40418Black 2nd Channel 6 40420
LINEAR 1101 CHART RECORDERSUSE WITH RECORDER MODEL: PART NO. PRICE
Model 1101 Recorder, 120V 1101Model 1101 Recorder, 240V 11020
Linear 1101, 100mm Specifi cationsChart Speeds: 1, 2, 3, 6, 10, 15, 20 & 30cm/min or hrInput Ranges: 1, 2, 5, 10, 20, 50mV and0.1, 0.2, 0.5, 1,
2, 5V Full ScaleInput Impedance: 2.5MΩ, FixedZero Adjust: ±100%Accuracy: ±0.5% Full ScaleResponse Time: <0.25sec, Full ScalePower: 120/240VAC, 50/60Hz, 8WDimensions:
4.4" H x 8.6" W x 10.5" D(11.2cm H x 21.8cm W x 26.7cm D)
Weight: 6.5lbs (3kg)Other Features: Override Event MarkerWarranty: 1 Year Parts, 90 days labor
LINEAR ACCESSORIESDESCRIPTION QTY. PART NO. PRICE
Linear Chart PaperUse with Recorder Model: 1 0100-0011140, 141, 143, 1101, 1110, 2010 6 001106
24 0011245" Chart paper (Metric)Use with Recorder Model: 1 0100-0017155, 156, 157 6 001706
24 001724
Linear Model 1101, 100mm Chart Recorder• High performance data recording at an economical
price• Compact design saves bench space• Patented pulse modulated servo system provides
reliable and reproducible recording
For recorder pens and chart paper (strip chart rolls, Z-fold, and thermal paper) for many popular recorders, request sheet #002705D.
The BD 11E nd12E models have enhanced capabilities with electrical pen lift and remote control features.