Evaluation of Karst Water Quality For Vaname Shrimp (Litopenaeus vannamei) Culture Medium DM Wildan1*, R Affandi1, NTM Pratiwi1, M Krisanti1, IP Ayu1, A Iswantari1 1Living Aquatic Resources Management, Faculty of Fisheries and Marine Science, Bogor Agricultural University.

*Corresponding email: dudi.mw@gmail.com

Abstract Vaname shrimp (Litopenaeus vannamei) is one of the excellent fishery commodities in Indonesia. Vaname shrimp farming can be conducted in low salinity water. Low salinity water sources which could be used as culture media is water karst or limestone water because it has a high mineral. The research was aimed to evaluate the suitability of karst water conditions for vaname shrimp culture media. Research was conducted in May and August 2016. Water sampling was conducted in several locations; Ancol-Jakarta (sea water), Ciseeng-Bogor (karst water salinity), Ciampea-Bogor (karst freshwater), and Situ Gede Bogor (freshwater). The results showed that karst water of Ciseeng and Ciampea were not suitable to be used as culture medium of vaname shrimp, compared with SNI 01-7246-2006. Ozonation and aeration treatments were able to improve the quality of karst water up to approach the living quality standard of vaname shrimp media. Karst water of Ciseeng and Ciampea could not directly use as vaname shrimp culture media. It is needed water quality treatment of ozonation and aeration of karst water to improve water quality. Keywords: evaluation, karst water, media, vaname shrimp

Research Methods

Karst water quality evaluation

Such as : Parameter salinity , pH, dissolved oxygen, temperature, DO meter, electrical conductivity (EC), total hardness, SO4

- (sulfate), alkalinity, NH3-

(ammonia), Ca, Mg, K, and H2S.

Karst water quality

treatment

Data analysis

comparing the overall results of the analysis of quality of karst water to sea water as its natural habitat, as well as to the Indonesian National Standard 01-7246-2006 for vaname shrimp aquaculture.

Differences in karst water quality before and after the modification were analyzed using t-test.

Research Location and Sampling Site

Stages of Research

1. Ozonation and aeration during 24 hours. 2. Prior to ozonation and aeration, the water was filtered using a sieve with a

mesh size of 25 microns. 3. Left a few hours until the odor generated by ozone treatment disappeared.

Acknowledgements Thanks to the Ministry of Research, Technology and Higher Education which has provided grants and also BPPTN and LPPM as a facilitator so that the study can be conducted well. This research is part of a series of study activities entitled "Ecological Potential and Karst Water Utilization as Rich in Mineral Growing Media for Aquatic Micro Biota".

Conclusion Ciampea Ciseeng karst water and can be used as vaname shrimp culture media after going through ozonation and aeration treatment. Treatment ozonation and aeration can improve the quality of karst water.

Introduction

Vaname shrimp (Litopenaeus vannamei)

vaname shrimp (euryhalin)

Sea, lakes, rivers, wetlands and

reservoirs

Vaname shrimp culture in

the low salinity waters

vaname shrimp experience stress

cannot maintain the osmotic

pressure (Mantel et al, 1983)

necessary source of fresh water that has a high mineral content

Karst waters (Hartmann et al, 2014)

(Ekmekci 2005)

contain minerals dominated by calcium,

magnesium, and carbon

Results and Discussion 1. Physical and chemical conditions of the water

2. Comparison of physical and chemical parameters of water karst water quality for shrimp by SNI 01-7246-2006

3. Karst water quality modification

Parameter Unit Water Source

Seawater Ciseeng Ciampea Situ Gede

Physics

Temperature ºC 28,000±0,820 39,100±1,890 26,500±0,880 28,500±0,510

Conductivity µS/cm 47740,00±1368,89

0

41972,500±2784,09

0 433,000±13,520 267,000±123,600

Chemistry

Dissolved Oxygen mg/l 4,400±0,320 0,275±0,150 4,500±0,260 4,050±0,240

Salinity ppt 33,250±0,960 33,250±0,960 0 0

pH - 8,120±0,080 6,352±0,420 6,680±0,170 6,460±0,390

Hardness mg/l CaCO3 4103,900±622,580 8458,450±5262,32 84,080±30,840 62,560±18,590

Alkalinity mg/l 290,000±42.426 1599,000±202,840 267,750±5,440 51,000±6,000

NH3- mg/l 0,210±0,180 3,160±0,020 0,180±0,040 0,415±0,270

H2S mg/l <0,001 <0,001 <0,001 <0,001

Ca mg/l 652,460±99,470 2904,572±575,790 186,880±61,050 14,040±7,770

Mg mg/l 907,460±78,400 354,181±9,440 3,870±1,280 2,670±0,220

K mgCaCO3/l 329,550±134,410 250,950±18,250 0,370±0,250 1,140±0,120

SO4 mg/l 1234,620±132,920 8.360±0.26 5,270±0,760 8,470±0,520

Parameter Unit Ciseeng karst water Ciampea karst water SNI 01-7246-2006

Temperature ºC 39,100±1,890 26,500±0,880 28,500-31,500

Dissolved Oxygen mg/l 0,275±0,150 4,500±0,260 >3,500

Salinity ppt 33,250±0,960 0.00 15,000-25,000

pH - 6,353±0,420 6,680±0,170 7,500-8,500

Alkalinity mg/l 1599,000±202,840 267,750±5,440 100,000-150,000

NH3- mg/l 3,160±0,020 0,180±0,040 <0,010

H2S mg/l <0,001 <0,001 <0,001

Parameter Unit Ciseeng karst water Ciampea karst water SNI 01-7246-2006

Temperature ºC 27,15±0,129 27,250±0,058 28,500-31,500

Dissolved Oxygen mg/l 5,15±0,129 5,200±0,183 >3,500

Salinity Ppt 33,250±0,960 0.000 15,000-25,000

pH - 8,0125±102 7,673±0,077 7,500-8,500

Alkalinity mg/l 204,000±19,114 54,750±6,397 100,000-150,000

NH3- mg/l 2,664±0,135 0,056±0,059 <0,010

H2S mg/l <0,001 <0,001 <0,001

(p<0,05) Explanation :

Ekmekci M. 2005. Karst in Turkish Thrace: Compatibility between Geological History and Karst Type. Turkish Journal of Earth Sciences.Vol (14): 73-90 Hartmann A, Goldscheider N, Wagener T, Lange BJ, Weiler M. 2014. Karst Water Resources In A Changing World: Review Of Hydrological Modeling

Approaches, Rev. Geophys. Vol (52): 218–242 Mantel LH, Farmer LL. 1983. Osmotic and ionic regulation. In: L. H. Mantel (ed), The Biology of Crustacea, Vol. 5, Internal Anatomy and Physiological

Regulation. Academic Press, New York.

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