essentials of gliomas
TRANSCRIPT
Essentials of gliomas-and their
mangement.Dr. Parag Moon
Senior resident,Dept. of Neurology
GMC, Kota
Classification of brain tumors
CNS tumors can primarily be divided into Primary -originates in the brain (solitary, nodular) Secondary-made up of cells that have spread
(metastasized) to the brain from somewhere else in the body.
Multiple in number, prior h/o cancer , edema +, located at gray white interface.
May lodge into - Brain parenchyma – most common area of
metastases- Leptomeninges – pia mater & arachnoid mater- Dural space
WHO classification of brain tumors
Tumors of Neuroepithelial Tissue Tumors of Cranial and Spinal Nerves Tumors of Meninges Lymphomas and Hematopoietic
Neoplasm Germ Cell Tumor Tumors of Sellar Regions (pituitary
/craniopharyngioma) Metastatic Tumors
Many grading systems (e.g., Kernohan, St. Anne-Mayo, and Ringertz systems)
Most of these grading systems share an assessment of nuclear abnormalities, mitoses, endothelial proliferation, and necrosis
WHO Grading :
WHO grade I – low proliferative potential. Possible cure with surgery alone
WHO grade II – infiltrating, but low in mitotic activity. Can recur and progress to other grades
WHO grade III – Histologic evidence of malignancy (mitotic activity), infiltrative, anaplastic
WHO grade IV – mitotically active, necrosis, rapid pre and post-surgical progression
Cell of origin can be1. Astrocytes2. Oligodendrocytes3. Ependymal cells4. Mixed Identified by glial fibrillary acidic protein
(GFAP) and S100
Gliomas
Gliomas-Most common (80%) primary malignant brain tumours.
Gliobastoma accounts for 54%. Most common is astrocytoma (including
gliobastoma)-76%. Slight male preponderance. More common in whites.
Epidemiology
Aetiopathology Exposure to ionizing radiations is the only
definite risk factor They are common in;
NF type I (15 – 20% develop LGGs) NF type II Tuberous sclerosis Li-Fraumeni syndrome
Environmental exposure to Nitrites/Nitrates (nitrosamines/amides)
Radiofrequency radiation. Electromagnetic field radiation(EMF)
Allergies/IgE levels association Vit. E and C consumption. Head trauma Tobacco, alcohol consumption p53 gene mutation (a consistent finding) 1p/19q mutations in tumours transforming
to high grade
Well circumscribed both grossly and radiologically.
More common in cerebellum, third ventricle, hypothalamus, optic nerve, spinal cord, dorsal brainstem.
Cerebellar-large fluid filled cyst with enhancing nodule.
Hyothalamus, optic nerve- solid tumours. Outcome excellent (80% 20-year survival)
Pilocytic astrocytoma(WHO grade I)
Biphasic pattern- compact pilocytic areas with interspersed microcytic, loose and spongy areas.
Rosenthal fibres Mulberry shaped eosinophilic granular
inclusion Pilomyxoid astrocytoma-variant, more
aggressive, affects children younger than 3 years
Pilocytic astrocytoma(WHO grade I)
Fig 2.4B: Axial T1 Wtd. MRIFig 2.4A: Axial T2 Wtd. MRI Fig 2.4C: Post-Contrast Axial T1 Wtd. MRIA large cystic tumor (yellow arrow) with a mural enhancing nodule (red arrow) is seen within the left cerebellar hemisphere.
DIAGNOSIS: PILOCYTIC ASTROCYTOMA
• Grade I Astrocytoma (WHO Classification)
• Children and young adults
• Imaging Features: cyst within enhancing tumor nodule.
Diffuse astrocytoma (WHO grade II) Well differentiated, low grade, simply
astrocytoma. Median age at diagnosis-35 years. Brainstem gliomas-childhood Ill defined non enhancing cerebral masses. Grossly poorly circumscribed. Lacks mitotic activity, microvascular
proliferation and necrosis Low MIB(Ki-67) proliferative index.
90%-mutation in IDH1 and IDH2 encoding for isocitrate dehydrogenase in citric acid cycle.
IDH1 mutation-prognostic significance 50%- loss of 17p and mutation in TP53.
Diffuse astrocytoma (WHO grade II)
Axial T1 Wtd. MRI Axial Flair MRI Post-Contrast Axial T1 Wtd. MRI
Non-enhancing tumor (arrow) involving the right temporal lobe.
GRADE II ASTROCYTOMA (LOW GRADE)
• Children and young adults
• Imaging Features: Non-enhancing tumors. Calcification can be seen.
Anaplastic astrocytoma (WHO grade III) Mean age- fouth or fifth decade Some contrast enhancement on MRI. More cellular than grade II Presence of mitotic figures. High Ki67/MIB index High incidence of progression to GBM High frequency of TP53, IDH1, IDH2, RB, PTEN EFGR mutation-worse prognosis. Relative survival at 2 years-40% and at 5
years-27%
Fig 2.2B: Axial T1 Wtd. MRIFig 2.2A: Axial Flair MRI Fig 2.2C: Post-Contrast Axial T1 Wtd. MRI Fig 2.2D: Post Contrast Coronal T1 Wtd. MRIAn ill-defined non-enhancing tumor (yellow arrows) is seen in the left parietal lobe with spotty areas of enhancement (red arrows).
DIAGNOSIS: Anaplastic Astrocytoma
• Grade III Astrocytoma (WHO Classification)
• Usually seen between 40 – 60 years of age
• Imaging Features: Ill-defined non-enhancing tumor with or without feeble enhancement.
Glioblastoma (WHO grade IV) Previously known as glioblastoma multiforme Peak age of onset is 50-60yrs. Common in deep white matter, basal
ganglia, thalamus, rarely in cerebellum Grossly may appear circumscribed Microscopic infiltrates widely, often to other
hemisphere via corpus callosum. Multifocal Extracranial metastasis rare. Survival 1-1.5 yrs after treatment.
Central yellow or white zone of necrosis and hemorrhage surrounded by hyperemic ring of endothelial hyperplasia.
Surrounded by edematous brain (mixture of vasogenic edema, tumour infiltrates and gliosis)
TP53, IDH1 and IDH2 mutation less common in primary.
30-40% have EFGR mutation MGMT mutation- favorable prognosis.
Glioblastoma (WHO grade IV)
Primary vs Secondary GBM
Primary GBM◦ Develops de novo from
glial cells◦ Accounts for > 90% of
biopsied or resected cases◦ Clinical history of 6 months◦ Occurs in older patients
(median age: 60 years)◦ EFGR mutation
Secondary GBM◦ Develops from low-grade or
anaplastic astrocytoma ~ 70% of lower grade
gliomas develop into advanced disease within 5-10 years of diagnosis
◦ Comprises < 5% of GBM cases
◦ Occurs in younger patients (median age: 45 years)
◦ TP53, IDH1, IDH2
Fig 2.1B: Axial T1 Weighted (Wtd.) MRI
Fig 2.1A: Axial Flair MRI Fig 2.1C: Post-Contrast Axial T1 Wtd. MRIAn irregular enhancing ring lesion (arrow) is seen involving the left parietal lobe. Tumor is associated with
edema (E) best noticed on FLAIR image (A).
DIAGNOSIS: GLIOBLASTOMA
E
• Grade IV Astrocytoma (WHO Classification)
• Older Patient
•Imaging Features: Tumor with irregular peripheral enhancement with central necrosis.
Pleomorphic Xanthoastrocytoma (WHO grade II or III) Average age of diagnosis-26 yrs. Involve cerebral cortex and overlying
meninges most common in temporal lobe. Bizzare giant cells Xanthomatous cells-foamy lipid filled
astrocytes seen in 1/4th cases. 15-20% have malignant transformation. Survival 81% at 5 yrs and 70% at 10 yrs.
large well defined cystic mass in right temporo-parieto-occipital region with mild vasogenic oedema and mass effects. Post-contrast rim like enhancement of the cystic mass, with enhancing mural nodule
Associated with tuberous sclerosis Gross- elongated, sausage like or lobulated Candle guttering-identical smaller masses
on walls of ventricle. Rich vascularity Pseudo-rosettes Hydrocephalus TSC1 and TSC2 Inhibitors of mTOR found effective.
Subependymal giant cell astrocytoma (WHO grade I)
Hypo- to isointense to gray matter on T1, heterogeneously hyperintense on FLAIR , T2 punctate hypointensities corresponding to calcium with avid contrast enhancement.
Seen in young to middle aged adults. Most common in frontal lobe Histopath- uniform round nuclei, bland
chromatin, perinuclear halo-Fried egg Rich branching capillary network-Chicken
wire 50-80%-loss of 1p and 19q Favorable response to chemotherapy IDH1 and IDH2 mutation in grade II (84%)
and grade III (94%).
Oligodendroglioma (WHO grade II or III)
Most difficult to define Survival between astrocytoma and
oligodendroglioma. Usually grade and treated as
oligodendroglioma.
Oligoastrocytoma (WHO grade II or III)
Most common in first decade. Age<3 years have worse prognosis Children-infratentorial Adult-spinal Well circumscribed mass that compresses
and not infiltrates surrounding parenchyma May seed subarachnoid space in 5% Perivascular rosettes 22q deletion in NF2 mutation-spinal
ependymoma.
Ependymoma (WHO grade II or III)
Seen in filum terminale More common in adults Thin collagenous capsule Prognosis excellent if capsule intact May metastasize to lungs
Myxopapillary ependymoma WHO grade I)
Hypointense mass on T1 and hyperintense with some hypointense signal within due to hemorrhage within on T2
Seen in adults Small and incidental Glistening pearly white lobulated
intraventricular proteburance Most often in fourth ventricle May cause hydrocephalus Low proliferative index
Subependymoma (WHO grade I)
Classically (17%) starts in early morning and disappears soon after pt. gets up
Initially mild, becomes progressively more severe, frequent and of longer duration
Worse with Valsalva manoeuvre Associated with nausea,vomiting Forsyth, Posner(1993) showed tension-
type headache(77%), migraine-like headache (9%),14% had mixture of headaches could not be easily classified
Headache
Forsyth (1993)- commonest headache site was frontal region (68%) seen primarily in supratentorial tumours or with raised ICT.
73% of patients with infratentorial tumours had frontal, temporal or parietal headaches and 24% had nuchal and occipital.
Related to size of tumour and amount of midline shift.
Produced due to traction on pain-sensitive structures such as blood vessels, dura or obstruction of CSF pathways
Headache
Recommendation Level
A patient with new onset or recurrent headache uncharacteristic for that patient should also be imaged, particularly if there are focal neurological symptoms and signs.
III
Neuro-imaging in headache
Other symptoms Limb weakness Language disturbances Apraxia, agnosia Amnesia Depression Seizures- most common partial motor
often with secondary generalization Plateau waves- paroxysmal neurological
symptoms on standing
Computed Tomography (LGG)
◦ A discrete or diffuse hypodense to isodense mass lesion
◦ Minimal or no enhancement (except in 15 – 30% patients)
◦ Calcifications (Oligodendrogliomas/Oligoastrocytomas)
◦ Cystic changes (any histologic type)
Magnetic Resonance Imaging (LGG)
◦ Hypo- to Iso-intense on T1WI
◦ Hyperintense on T2WI
◦ Minimal- to NO gadolinium enhancement
(25 – 50% oligodendrogliomas are somewhat
enhanced)
◦ No significant mass effect
◦ Tendency to invade & reside in white matter
Oligodendrogliomas expands along gyri◦ Calcifications (20% lesions)
MRI (contnd) T1-weighted MRI with contrast may
underestimate the extent of an LGG The true extent is shown on the T2-
weighted sequences, Diffusion tensor MRI used as a marker
of glioma infiltration
Reduced NAA peaks Increased choline peak Increased choline/creatinine ratio Choline/creatinine peak>3:1 predicts high
grade tumour.
MR spectroscopy
Malignant Transformation:LGG transformation ranges from 17% to 73% in clinical studiesRisk of progression increases with tumour burdenGrowth rate of ≥ 8 mm per year Median survival of 5.16 yearsGrowth rate ≤ 8 mm/year median survival of ≥ 15 yearsResidual tumour after surgery is an important determinant
Treatment
Observation: Advantages:
No surgical morbidity Lesser cost of follow up treatment
Disadvantages: Loss of histological diagnosis Loss to follow up (quite frequent than reported) Risk of increased malignant transformation Risk of increasing tumour burden/neurodeficits
Supportive treatment Antiepileptics- leviteracetam preferred.
Prophylactic antiepileptics not recommended.
Enzyme inducers avoided. Steroids- Dexamethasone most commonly
used. Usual dose 8-16mg daily dose.
Surgical intervention: Open resection Open/stereotactic biopsy
Guided by patient’s clinical status, location of tumour & surgeon’s preference
Goals of surgery: Establishing a diagnosis Symptoms alleviation Decompression Tumour cytoreduction
Biopsy: Open/Image guided or stereotactic (if available) Indicated in high risk patients or when open surgery is
declined/deferred
Advantages: Minimally invasive Early identification of histologic type
Disadvantages: Morbidity/mortality with open biopsy Image guided biopsy may sample wrong site Stereotactic biopsy may be too small for a diagnosis
Surgical Resection: It is the principle mode of treatment in the following;
Whenever possible Mass effect Raised ICT Steroid resistant edema Threat of herniation CSF flow obstruction Seizure control Smaller tumours are less aggressive & better surgical
candidates
Surgery has limited role in following; Disseminated tumours Multifocal tumours Eloquent location
Extent of Resection:
◦ Recent evidence favouring early extensive resection
◦ Good life expectancy◦ Influencing malignant transformation ◦ Progression free survival & overall survival both
improves
Radiation:
Radiation for Pilocytic astrocytoma after surgery may be reserved until recurrence or deep lesions
Fractionated radiation of up to 45-60 Gy (more focussed, more good)
Hyperfraction protocol Provided to T2 abnormality and margin of 1.5 to 2 cm In sharply demarcated tumour 0.5cm margin suffice Adjvunt to surgery Malignant transformation may be treated with
radiation Steriotactic radiosurgery-useful in well circumscribed
tumour. Brachytherapy
Chemotherapy: PCV (Procarbazine, Lomustine, Vincristine) may have
a role in stabilising tumour growth Temozolomide may have a role for progressive
astrocytoma Oligodendroglioma- chemosensitive Recurrence of anaplastic astrocytoma and
glioblastoma multiforme
Temozolomide(Temodal)
Methylating agent Principal mechanism is causing damage to
DNA of tumour cell, leading to cell death Taken orally, rapidly absorbed Penetrates the blood-brain barrier TMZ 75 mg/m2 PO QD for 6 weeks, then 150-
200 mg/m2 PO QD on Days 1-5 every 28 days for 6 cycles
Used with radiotherapy
2nd line Chemotherapy for recurrence No consensus Low dose temozolomide (+/- procarbazine) Carboplatin BCNU/CCNU Bevacizumab (+/- Irinotecan) EFGR inhibitors (geftinib) PDGFR inhibitors (imatinib) Clinical trials if possible
Surgical Implantation of Chemotherapy Wafers: Gliadel®
BCNU(carmustine)-infused wafers
Implanted to tumour bed at time of surgery
Chemotherapy released to surrounding brain tissue over a period of 2 to 3 weeks
Clinical trials showed survival benefit
Prognosis
◦ Extremely young patients<3yrs or patients > 50 y◦ Large tumours that enhance◦ Short clinical history◦ Absent of mental changes◦ Cerebellar location◦ High grade◦ Completeness of surgical lesion◦ Evidence of progression on imaging studies
Clinical course is by no means benign as is manifested by histology & radiologic appearance
Aggressive early resection advised but NOT on the expense of patient’s quality of life
Diagnosis purely on the basis of radiology has a failure rate of up to 50%
Early histologic evidence of the diagnosis is paramount both for the surgeon & the patient
Chemo-radiotherapy can be delayed until recurrence or progression in low grade gliomas.
Conclusion
Clinical practice guidelines for management of gliomas: Clinical oncological society of Australia; Aug2009.
Malignant Gliomas in Adults;Patrick Y. Wen, Santosh Kesari:N Engl J Med 2008; 359:492-507
Guidelines on management of low-grade gliomas: report of an EFNS–EANO* Task Force; European Journal of Neurology 2010, 17: 1124–1133
References