enzymes models of action 2

29
Enzyme Action objective : to describe different models for ction and to investigate factors which contr an enzyme reaction 18 th October

Upload: thana-segar

Post on 29-Apr-2017

215 views

Category:

Documents


1 download

TRANSCRIPT

Page 1: Enzymes Models of Action 2

Enzyme ActionLearning objective: to describe different models forenzyme action and to investigate factors which control the rate of an enzyme reaction

18th October

Page 2: Enzymes Models of Action 2

Lock and Key

Page 3: Enzymes Models of Action 2

Lock and KeyHowever certain substances can bind to the enzyme at sites other than theActive site and modify its activity (inhibitors/co-factors)

Idea that the enzyme is flexible

Page 4: Enzymes Models of Action 2

Induced Fit

Page 5: Enzymes Models of Action 2

Enzyme reactions

enzyme + substrate enzyme-substrate complex

Page 6: Enzymes Models of Action 2

Enzyme reactions

enzyme + substrate enzyme-substrate complex

E +S ES

Page 7: Enzymes Models of Action 2

Enzyme reactions

enzyme + productenzyme-substrate complex

E +PES

enzyme + substrate enzyme-substrate complex

E +S ES

Page 8: Enzymes Models of Action 2

Enzyme activity

How fast an enzyme is workingRate of Reaction

Page 9: Enzymes Models of Action 2

Enzyme activity

How fast an enzyme is workingRate of Reaction

Rate of Reaction = Amount of substrate changed (or amount product formed) in a given period of time.

Page 10: Enzymes Models of Action 2

Rat

e of

Rea

ctio

n

Enzyme activity

Variable you are looking at

Page 11: Enzymes Models of Action 2

Enzyme activity

Four Variables

Page 12: Enzymes Models of Action 2

Enzyme activity

Four Variables

Temperature

pH

Enzyme Concentration

Substrate Concentration

Page 13: Enzymes Models of Action 2

Rat

e of

Rea

ctio

n

Temperature

Page 14: Enzymes Models of Action 2

Rat

e of

Rea

ctio

n

Temperature

0 20 30 5010 40 60

Page 15: Enzymes Models of Action 2

Rat

e of

Rea

ctio

n

Temperature

0 20 30 5010 40 60

40oC - denatures

5- 40oC Increase in Activity

<5oC - inactive

Page 16: Enzymes Models of Action 2

Effect of heat on enzyme activtyIf you heat the protein above its optimal temperature

bonds break meaning the protein loses it secondary and tertiary structure

Page 17: Enzymes Models of Action 2

Effect of heat on enzyme activty

Denaturing the protein

Page 18: Enzymes Models of Action 2

Effect of heat on enzyme activty

Denaturing the proteinACTIVE SITE CHANGES SHAPE

SO SUBSTRATE NO LONGER FITS

Even if temperature lowered – enzyme can’t regain its correct shape

Page 19: Enzymes Models of Action 2

Rat

e of

Rea

ctio

n

pH

Page 20: Enzymes Models of Action 2

Rat

e of

Rea

ctio

n

pH

1 3 42 5 6 7 8 9

Page 21: Enzymes Models of Action 2

Rat

e of

Rea

ctio

n

pH

1 3 42 5 6 7 8 9

Narrow pH optima

Page 22: Enzymes Models of Action 2

Rat

e of

Rea

ctio

n

pH

1 3 42 5 6 7 8 9

Narrow pH optima

WHY?

Page 23: Enzymes Models of Action 2

Rat

e of

Rea

ctio

n

pH

1 3 42 5 6 7 8 9

Narrow pH optima

Disrupt Ionic bonds - Structure

Effect charged residues at activesite

Page 24: Enzymes Models of Action 2

Rat

e of

Rea

ctio

n

Enzyme Concentration

Page 25: Enzymes Models of Action 2

Rat

e of

Rea

ctio

n

Enzyme Concentration

Enzyme Concentration

Page 26: Enzymes Models of Action 2

Rat

e of

Rea

ctio

n

Substrate Concentration

Page 27: Enzymes Models of Action 2

Rat

e of

Rea

ctio

n

Substrate Concentration

Substrate Concentration

Page 28: Enzymes Models of Action 2

Rat

e of

Rea

ctio

n

Substrate Concentration

Substrate Concentration

Active sites full- maximum turnover

Page 29: Enzymes Models of Action 2

This powerpoint was kindly donated to www.worldofteaching.com

http://www.worldofteaching.com is home to over a thousand powerpoints submitted by teachers. This is a completely free site and requires no registration. Please visit and I hope it will help in your teaching.