enhancement of strength characteristics of poorly graded soil by flyash and cement

4
International Journ Internat ISSN No: 245 @ IJTSRD | Available Online @ www Enhancement of Str Soil Kammampati Lax Gokul Group o ABSTRACT Fly Ash is the waste material, which is burning coal in Thermal Power Plants. as a stabilizer for soil due to its pozzo an inherent self-hardening property und conditions of moisture and compaction aims at increasing the strength of poorl by using Fly Ash and cement as adm percentage of Fly Ash without any utilized so as to reduce the cost of con this is a good method for disposal of it added in various percentages like 10, 2 percentages (% by volume). Initia Geotechnical properties of the soil s limit, plastic limit, shrinkage limit, Analysis, Specific Gravity, Free Unconfined Compressive Strength, OM were determined. Later on the stabilize tested for CBR (California Bearing Rati indirect measurement of strength. Afte experimental investigation it is observ addition of Fly Ash has shown maximum that to obtain much more strengths cem in 2, 5, 8 and 10 percentages to this op Fly Ash with soil. After that the soi tested for CBR (California Bearing Ra it’s feasibility in Flexible Pavement The strengths were checked in both U soaked conditions and at various curing and 28 Days. Among all proportions 30 % of the cement has shown maximum that it was decided as optimum mi strengths were obtained at 28 days cur all proportions of mixes so that this per as optimum curing period. Eventually work facilitates an economical, strong construction material for Flexible Pavem nal of Trend in Scientific Research and De tional Open Access Journal | www.ijtsr 56 - 6470 | Volume - 2 | Issue – 6 | Sep w.ijtsrd.com | Volume – 2 | Issue – 6 | Sep-Oct rength Characteristics of Poorl l by Flyash and Cement xman 1 , Ch. Damodhar Naidu 2 , Bothsa Kum 1 Student, 2 Assistant Professor of Institutions, Bobbili, Andhra Pradesh, India obtained after It can be used olanic effect or der favourable n. This project ly graded soils mixtures. Some additives was onstruction and t. Fly Ash was 20, 30 and 40 ally all basic such as liquid , Grain Size Swell Index, MC and MDD ed mixes were io) which is an er the detailed ved that 30% m strengths, so ment was added ptimum mix of il mixes were Ratio) to check constructions. Unsoaked and g periods like 7 0% Fly Ash+8 m strengths, so ix. Maximum ring period for riod is decided y this project g and durable ments. Keyword: Soil, Flyash, Cemen and UCS I. INTRODUCTION Soil is the cheapest availabl various construction-related connection, utilization of by-p suitable ingredients for geote necessary. Soil improvement soils in many places. Soils wit underlying heavy structures from the geotechnical enginee essential to overcome this pr the soil. Precautions can be t local soils, which can be mechanical or chemical stabili There are many methods stabilization. For chemical sta are many additives available gypsum and Fly Ash. Among the cheapest one. Soil stabili Ash has environmental b pollution of water and air that disposal near thermal power grade soils can be stabilized b Many research results have in an effective material and application to stabilize soft su of Fly Ash is prominent e reactivity, and the California soil-Fly Ash mixes soil pozzolanic reaction. Unconfin (UCS) increases with curing p mixtures, and this is primaril reaction. The increase in per Ash in Fly Ash-soil mixtures evelopment (IJTSRD) rd.com p – Oct 2018 2018 Page: 1542 ly Graded mari 1 nt (OPC 43 grade), CBR le material utilized for d purposes. In this products like Fly Ash as echnical construction is t is necessary for local th low bearing capacities are always problematic ering point of view. It is roblem by strengthening taken by modifying the e achieved either by ization. s available for soil abilization of soils, there e such as lime, cement, g all of them, Fly Ash is ization by means of Fly benefits in preventing can result from its mere plants. Most of the sub by means of admixtures. ndicated that Fly Ash is also has the potential ub-grade soils. The effect essentially through the bearing ratio (CBR) of increases due to the ned compressive strength periods for soil-Fly Ash ly due to the pozzolanic rcentage content of Fly leads to decrease in dry

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Fly Ash is the waste material, which is obtained after burning coal in Thermal Power Plants. It can be used as a stabilizer for soil due to its pozzolanic effect or an inherent self hardening property under favourable conditions of moisture and compaction. This project aims at increasing the strength of poorly graded soils by using Fly Ash and cement as admixtures. Some percentage of Fly Ash without any additives was utilized so as to reduce the cost of construction and this is a good method for disposal of it. Fly Ash was added in various percentages like 10, 20, 30 and 40 percentages by volume . Initially all basic Geotechnical properties of the soil such as liquid limit, plastic limit, shrinkage limit, Grain Size Analysis, Specific Gravity, Free Swell Index, Unconfined Compressive Strength, OMC and MDD were determined. Later on the stabilized mixes were tested for CBR California Bearing Ratio which is an indirect measurement of strength. After the detailed experimental investigation it is observed that 30 addition of Fly Ash has shown maximum strengths, so that to obtain much more strengths cement was added in 2, 5, 8 and 10 percentages to this optimum mix of Fly Ash with soil. After that the soil mixes were tested for CBR California Bearing Ratio to check its feasibility in Flexible Pavement constructions. The strengths were checked in both Unsoaked and soaked conditions and at various curing periods like 7 and 28 Days. Among all proportions 30 Fly Ash 8 of the cement has shown maximum strengths, so that it was decided as optimum mix. Maximum strengths were obtained at 28 days curing period for all proportions of mixes so that this period is decided as optimum curing period. Eventually this project work facilitates an economical, strong and durable construction material for Flexible Pavements. Kammampati Laxman | Ch. Damodhar Naidu | Bothsa Kumari "Enhancement of Strength Characteristics of Poorly Graded Soil by Flyash and Cement" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-2 | Issue-6 , October 2018, URL: https://www.ijtsrd.com/papers/ijtsrd18888.pdf Paper URL: http://www.ijtsrd.com/engineering/civil-engineering/18888/enhancement-of-strength-characteristics-of-poorly-graded-soil-by-flyash-and-cement/kammampati-laxman

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Page 1: Enhancement of Strength Characteristics of Poorly Graded Soil by Flyash and Cement

International Journal of Trend in

International Open Access Journal

ISSN No: 2456

@ IJTSRD | Available Online @ www.ijtsrd.com

Enhancement of Strength Characteristics of Poorly GradedSoil by Flyash a

Kammampati Laxman

Gokul Group o

ABSTRACT Fly Ash is the waste material, which is obtained after burning coal in Thermal Power Plants. It can be used as a stabilizer for soil due to its pozzolanic ean inherent self-hardening property under favourable conditions of moisture and compaction. This project aims at increasing the strength of poorly graded soils by using Fly Ash and cement as admixtures. Some percentage of Fly Ash without any additutilized so as to reduce the cost of construction and this is a good method for disposal of it. Fly Ash was added in various percentages like 10, 20, 30 and 40 percentages (% by volume). Initially all basic Geotechnical properties of the soil suchlimit, plastic limit, shrinkage limit, Grain Size Analysis, Specific Gravity, Free Swell Index, Unconfined Compressive Strength, OMC and MDD were determined. Later on the stabilized mixes were tested for CBR (California Bearing Ratio) which is aindirect measurement of strength. After the detailed experimental investigation it is observed that 30% addition of Fly Ash has shown maximum strengths, so that to obtain much more strengths cement was added in 2, 5, 8 and 10 percentages to this optimum Fly Ash with soil. After that the soil mixes were tested for CBR (California Bearing Ratio) to check it’s feasibility in Flexible Pavement constructions. The strengths were checked in both Unsoaked and soaked conditions and at various curing periodsand 28 Days. Among all proportions 30% Fly Ash+8 % of the cement has shown maximum strengths, so that it was decided as optimum mix. Maximum strengths were obtained at 28 days curing period for all proportions of mixes so that this period is decideas optimum curing period. Eventually this project work facilitates an economical, strong and durable construction material for Flexible Pavements.

International Journal of Trend in Scientific Research and Development

International Open Access Journal | www.ijtsrd.com

ISSN No: 2456 - 6470 | Volume - 2 | Issue – 6 | Sep

www.ijtsrd.com | Volume – 2 | Issue – 6 | Sep-Oct 2018

Strength Characteristics of Poorly GradedSoil by Flyash and Cement

Kammampati Laxman1, Ch. Damodhar Naidu2, Bothsa Kumari1Student, 2Assistant Professor

Gokul Group of Institutions, Bobbili, Andhra Pradesh, India

Fly Ash is the waste material, which is obtained after burning coal in Thermal Power Plants. It can be used as a stabilizer for soil due to its pozzolanic effect or

hardening property under favourable conditions of moisture and compaction. This project aims at increasing the strength of poorly graded soils by using Fly Ash and cement as admixtures. Some percentage of Fly Ash without any additives was utilized so as to reduce the cost of construction and this is a good method for disposal of it. Fly Ash was added in various percentages like 10, 20, 30 and 40 percentages (% by volume). Initially all basic Geotechnical properties of the soil such as liquid limit, plastic limit, shrinkage limit, Grain Size Analysis, Specific Gravity, Free Swell Index, Unconfined Compressive Strength, OMC and MDD were determined. Later on the stabilized mixes were tested for CBR (California Bearing Ratio) which is an indirect measurement of strength. After the detailed experimental investigation it is observed that 30% addition of Fly Ash has shown maximum strengths, so that to obtain much more strengths cement was added in 2, 5, 8 and 10 percentages to this optimum mix of Fly Ash with soil. After that the soil mixes were tested for CBR (California Bearing Ratio) to check it’s feasibility in Flexible Pavement constructions. The strengths were checked in both Unsoaked and soaked conditions and at various curing periods like 7 and 28 Days. Among all proportions 30% Fly Ash+8 % of the cement has shown maximum strengths, so that it was decided as optimum mix. Maximum strengths were obtained at 28 days curing period for all proportions of mixes so that this period is decided as optimum curing period. Eventually this project work facilitates an economical, strong and durable construction material for Flexible Pavements.

Keyword: Soil, Flyash, Cement (and UCS I. INTRODUCTION Soil is the cheapest available various construction-related purposes. In this connection, utilization of by-products like Fly Ash as suitable ingredients for geotechnical construction is necessary. Soil improvement is necessary for local soils in many places. Soils with low bearing capacities underlying heavy structures are always problematic from the geotechnical engineering point of view. It is essential to overcome this problem by strengthening the soil. Precautions can be taken by modifying the local soils, which can be achieved either by mechanical or chemical stabilization. There are many methods available for soil stabilization. For chemical stabilization of soils, there are many additives available such as lime, cement, gypsum and Fly Ash. Among all of them,the cheapest one. Soil stabilization by means of Fly Ash has environmental benefits in preventing pollution of water and air that can result from its mere disposal near thermal power plants. Most of the sub grade soils can be stabilized by meanMany research results have indicated that Fly Ash is an effective material and also has the potential application to stabilize soft subof Fly Ash is prominent essentially through the reactivity, and the California soil-Fly Ash mixes soil increases due to the pozzolanic reaction. Unconfined compressive strength (UCS) increases with curing periods for soilmixtures, and this is primarily due to the pozzolanic reaction. The increase in percentage content of Fly Ash in Fly Ash-soil mixtures leads to decrease in dry

Research and Development (IJTSRD)

www.ijtsrd.com

6 | Sep – Oct 2018

Oct 2018 Page: 1542

Strength Characteristics of Poorly Graded

othsa Kumari1

Cement (OPC 43 grade), CBR

Soil is the cheapest available material utilized for related purposes. In this

products like Fly Ash as suitable ingredients for geotechnical construction is necessary. Soil improvement is necessary for local

with low bearing capacities underlying heavy structures are always problematic from the geotechnical engineering point of view. It is essential to overcome this problem by strengthening the soil. Precautions can be taken by modifying the

h can be achieved either by mechanical or chemical stabilization.

There are many methods available for soil stabilization. For chemical stabilization of soils, there are many additives available such as lime, cement, gypsum and Fly Ash. Among all of them, Fly Ash is the cheapest one. Soil stabilization by means of Fly Ash has environmental benefits in preventing pollution of water and air that can result from its mere disposal near thermal power plants. Most of the sub grade soils can be stabilized by means of admixtures. Many research results have indicated that Fly Ash is an effective material and also has the potential application to stabilize soft sub-grade soils. The effect of Fly Ash is prominent essentially through the reactivity, and the California bearing ratio (CBR) of

Fly Ash mixes soil increases due to the pozzolanic reaction. Unconfined compressive strength (UCS) increases with curing periods for soil-Fly Ash mixtures, and this is primarily due to the pozzolanic

rcentage content of Fly soil mixtures leads to decrease in dry

Page 2: Enhancement of Strength Characteristics of Poorly Graded Soil by Flyash and Cement

International Journal of Trend in Scientific Research and Development (IJTSRD) ISSN: 2456

@ IJTSRD | Available Online @ www.ijtsrd.com

unit weight, which is attributed to the low specific gravity of Fly Ash. The addition of a small percentage of cement to soil-Fly Ash mixture increases the unconfined compressive strength value. of cement on the behaviour of sandy soil has been studied and it has been reported that the addition of cement increases the stiffness as well as peak strength. Brittle behaviour is more marked in soilAsh-cement mixes than in soil-fly-ashwith concurrent increase in UCS value. low dry unit weight and exerts less lateral earth pressure. The use of class F Fly Ash amended soils as highway base materials has been investigated. Some researchers have suggested that the performance analysis of Fly Ash should be based upon laboratory tests such as index properties, compaction, unconfined compressive strength and CBR tests of a specific soil. The strength approach can be applied to estimate the optimum mixture design. The literature review has indicated that the strength gain due to stabilization depends upon several factors: Fly Ash content mounding water content, compaction delay and curing period. The use of Fly Ash in geotechnical engineering applications such as construction of highway embankments, different layers of road pavement etc. is increasing. In view of the above, the objective of this study was to investigate the strength properties of soil-Fly Ash and soil-Fly Ash-cement mixtures. II. MAJOR FORMAT GUIDELINESMETHODOLOGY: Materials Used: Fly Ash: For the present study Fly Ash was collected from the National Thermal Power Corporation (NTPC), which is located at paravada in Visakhapatnam. Cement: The cement used in this project work is OPC43 grade (Nagarjuna cement) which is collected from the local construction site. LABORATORY TESTING: The following tests were conducted on poorly graded sand. The index and engineering properties of poorly graded sand were determined. 1. Grain Size Analysis 2. Atterberg Limits 3. Specific Gravity 4. Unconfined Compressive Strength Test5. California Bearing Ratio Test

International Journal of Trend in Scientific Research and Development (IJTSRD) ISSN: 2456

www.ijtsrd.com | Volume – 2 | Issue – 6 | Sep-Oct 2018

unit weight, which is attributed to the low specific gravity of Fly Ash. The addition of a small percentage

Fly Ash mixture increases the value. The influence

of cement on the behaviour of sandy soil has been studied and it has been reported that the addition of cement increases the stiffness as well as peak strength. Brittle behaviour is more marked in soil-Fly

ash-fibre mixes value. Fly Ash has

low dry unit weight and exerts less lateral earth pressure. The use of class F Fly Ash amended soils as highway base materials has been investigated. Some

ggested that the performance analysis of Fly Ash should be based upon laboratory tests such as index properties, compaction, unconfined compressive strength and CBR tests of a specific soil. The strength approach can be applied to estimate the

ure design. The literature review has indicated that the strength gain due to stabilization depends upon several factors: Fly Ash content mounding water content, compaction delay and curing

The use of Fly Ash in geotechnical engineering ns such as construction of highway

embankments, different layers of road pavement etc. is increasing. In view of the above, the objective of this study was to investigate the strength properties of

cement mixtures.

FORMAT GUIDELINES

For the present study Fly Ash was collected from the National Thermal Power Corporation (NTPC), which is located at paravada in

The cement used in this project work is ) which is collected

The following tests were conducted on poorly graded sand. The index and engineering properties of poorly

essive Strength Test

6. Free Swell Index 7. Mini compaction. CEMENT TEST 1. Specific Gravity of Cement 2. Initial and Final setting Time Test on Cement 3. Standard Consistency Test III. RESULTS & DISCUSSIONSPHYSICAL PROPERTIES: parameters were determined for soil fly ash and cement

Property of the soilColour

Specific GravityUnconfined Compressive Strength (UCS)

kg/cm2 Liquid limit (%)Plastic limit (%)Shrinkage limit Plasticity index

California Bearing Ratio(CBR)Grain Size Distribution

a) Coarse sand (%)b) Medium sand (%)

c) Fine Sand (%)d) Silt& clays (%)Free swell index

Property of the Fly Ash

Appearance Colour

Specific Gravity Liquid limit (%) Plastic limit (%)

Wet sieve analysis a) % of passing through 75 micron

b) Retained (%) Free swell index (%)

S.

NO PROPERTY OF THE

CEMENT1 SPECIFIC GRAVITY2 NORMAL CONSISTENCY3 INTIAL SETTING TIME4 FINAL SETTING TIME

The Particle size Distribution of the soil shown below in the Figure

International Journal of Trend in Scientific Research and Development (IJTSRD) ISSN: 2456-6470

Oct 2018 Page: 1543

Specific Gravity of Cement Initial and Final setting Time Test on Cement Standard Consistency Test

RESULTS & DISCUSSIONS : the basic geotechnical

parameters were determined for soil fly ash and

Property of the soil Value Brown

Gravity 2.68 Unconfined Compressive Strength (UCS)

0.3

Liquid limit (%) 26.50 Plastic limit (%) 22.60

13.4 3.90

California Bearing Ratio(CBR) 2.6 Grain Size Distribution

Coarse sand (%) 3.06 Medium sand (%) 36.36

Fine Sand (%) 57.60 Silt& clays (%) 2.90

Free swell index 4.20%

Property of the Fly Ash Value Powder form

Grey 2.3 26

Non plastic

through 75 micron 65 35

9

PROPERTY OF THE CEMENT VALUE

SPECIFIC GRAVITY 3.1 NORMAL CONSISTENCY 31% INTIAL SETTING TIME 40MIN FINAL SETTING TIME 160MIN

The Particle size Distribution of the soil sample is as

Page 3: Enhancement of Strength Characteristics of Poorly Graded Soil by Flyash and Cement

International Journal of Trend in Scientific Research and Development (IJTSRD) ISSN: 2456

@ IJTSRD | Available Online @ www.ijtsrd.com

Co efficient of uniformity Cu = 1.375 Co efficient of Curvature Cc = 0.752 Soil Designation” Poorly Graded Sand (SP) The Index Properties of the Soil sample were tested and the results are as shown below The liquid Limit of the sample was determined using Digital cone penetrometer apparatus i.e Uppal’s Method the curve is as shown in the Figure

COMPACTION CHARACTERISTICS:Optimum Moisture Content & Maximum Dry Density The parameters like OMC (Optimum Moisture Content) and MDD (maximum Dry Density) were determined for the soil sample. The parameters were found by using MINI COMPACTION apparatus which is suggested by Professor A. Sridharan and Professor P.V.Sivapullaiah of IISC Bangalore. VARIATION OF CBR WITH CHANGE IN % OF FLY ASH AT 7 DAYS CURING IN BOTH SOAKED AND UN SOAKED CONDITIONS:

S. NO

% OF FLY ASH

% OF CBR AT 2.5 MM

% OF CBR AT

(SOAKED)1 10 8 2 20 9.6 3 30 11.2 4 40 9.6

International Journal of Trend in Scientific Research and Development (IJTSRD) ISSN: 2456

www.ijtsrd.com | Volume – 2 | Issue – 6 | Sep-Oct 2018

Poorly Graded Sand (SP) “

The Index Properties of the Soil sample were tested

liquid Limit of the sample was determined using

i.e Uppal’s Method the curve is as shown in the

COMPACTION CHARACTERISTICS: Optimum Moisture Content & Maximum Dry

The parameters like OMC (Optimum Moisture Content) and MDD (maximum Dry Density) were determined for the soil sample. The parameters were found by using MINI COMPACTION apparatus which is suggested by Professor A. Sridharan and

f IISC Bangalore.

OF CBR WITH CHANGE IN % OF FLY ASH AT 7 DAYS CURING IN BOTH SOAKED AND UN SOAKED CONDITIONS:

% OF CBR AT 2.5 MM

(SOAKED) 3.5 4

4.8 4.6

VARIATION OF CBR WITH CHANGE IN % OF CEMENT AT 28 DAYS CURING IN BOTH SOAKED AND UN SOAKED CONDITIONS:

IV. CONCLUSIONS � It is observed from all experimental results that

the certain proportions of Fly Ash added to the soil improved the CBR values.

� The optimum content of the Fly Ash was the soil at both Un soaked & soaked conditions.

� The optimum content of the cement was decided as 8% for optimum mix of Fly Ashsoaked & soaked conditions.

� Maximum strengths were obtained at 28 days curing period for all proportions of mixes so that this period is decided as optimum curing period

� The percentage increase in Fly Ash increasmaximum dry density and decreases the optimum moisture content.

� The percentage increases in cement and optimum percentage of Fly Ash (30%) combination leads to the increase in maximum dry density and decreases the moisture content.

S. NO

% OF CEMENT+30

%FA

CBR AT 2.5 MM

1 2

2 5

3 8

4 10

International Journal of Trend in Scientific Research and Development (IJTSRD) ISSN: 2456-6470

Oct 2018 Page: 1544

OF CBR WITH CHANGE IN % OF CEMENT AT 28 DAYS CURING IN BOTH SOAKED AND UN SOAKED CONDITIONS:

experimental results that the certain proportions of Fly Ash added to the soil improved the CBR values.

The optimum content of the Fly Ash was 30% for the soil at both Un soaked & soaked conditions.

The optimum content of the cement was decided for optimum mix of Fly Ash-soil at both un

soaked & soaked conditions.

Maximum strengths were obtained at 28 days curing period for all proportions of mixes so that this period is decided as optimum curing period

The percentage increase in Fly Ash increases the maximum dry density and decreases the optimum

The percentage increases in cement and optimum percentage of Fly Ash (30%) combination leads to the increase in maximum dry density and decreases the moisture content.

% OF CBR AT 2.5 MM

(US)

% OF CBR AT 2.5 MM

(S) 16 9

24 15

38 23

40 25

Page 4: Enhancement of Strength Characteristics of Poorly Graded Soil by Flyash and Cement

International Journal of Trend in Scientific Research and Development (IJTSRD) ISSN: 2456

@ IJTSRD | Available Online @ www.ijtsrd.com

REFERENCE 1. Dili Kumar, Ashish Gupta & Neetesh Kumar “

Some Geotechnical Properties of Coal Fly Ash and Sand Mixtures with Different Ratio Highway & Embankments”

2. K. Vaidya, Chore H. S, P. Kousitha, S.Ukrande “ Geotechnical Characterization Cement Fly Ash -Fibers Mix.

3. K. Vaidya, Chore H. S, P. Kousitha, S.Ukrande “ Geotechnical Characterization Cement Fly Ash -Fibers Mix”.

4. Chayan Gupta ,Dr. Ravi Kumar Sharma “Influence of Marble Dust, Fly Ash a

International Journal of Trend in Scientific Research and Development (IJTSRD) ISSN: 2456

www.ijtsrd.com | Volume – 2 | Issue – 6 | Sep-Oct 2018

Dili Kumar, Ashish Gupta & Neetesh Kumar “ Some Geotechnical Properties of Coal Fly Ash

Sand Mixtures with Different Ratio using in

Kousitha, S. K. Geotechnical Characterization Cement -

Kousitha, S. K. Geotechnical Characterization Cement -

Chayan Gupta ,Dr. Ravi Kumar Sharma “Influence of Marble Dust, Fly Ash and Beas

Sand on Sub Grade Characteristics of Expansive Soil”,

5. Ajanta Kalita “Experimental Study on Strength Behaviour of Cement Blended SoilMixes”.

6. Asce, 128(6): 462-472. Kaniraj, S.Havanagi, V. G. (1999a). “Geotechnical Characteristics of Fly AshGeotechnical Engineering Journal, 30(2):129

7. Kaniraj, S. R. And Gayathri, V. (2003). “Factors Influencing The Strength Of CementBase Courses”, Journal Of Transportation Engineering, Asce, 129(5): 538

International Journal of Trend in Scientific Research and Development (IJTSRD) ISSN: 2456-6470

Oct 2018 Page: 1545

Sand on Sub Grade Characteristics of Expansive

Ajanta Kalita “Experimental Study on Strength of Cement Blended Soil-Fly Ash

472. Kaniraj, S. R. And G. (1999a). “Geotechnical

Fly Ash-Soil Mixtures”, neering Journal, 30(2):129-146.

R. And Gayathri, V. (2003). “Factors Influencing The Strength Of Cement Fly Ash Base Courses”, Journal Of Transportation Engineering, Asce, 129(5): 538-548.