ENHANCEMENT OF EFFICIENCY OF ETHANOL PRODUCTION FROM JERUSALEM ARTICHOKE

Quang D. Nguyen, Kálmán Dénes, Csilla Farkas, Ágoston Hoschke, Judit M. Rezessy-SzabóThe 2nd International Symposium „Vera Johanides“ 2013

Production of bioethanol in EU-27 - 2009(million liters)

Source: www.biofuels-platform.ch

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Total: 3703

Productivity of ethanol using different raw materials

Plant Main carbohydrate Productivity (l/ha)

Sugarcane saccharose 8000

Sugar beet saccharose 3300Jerusalem artichoke

inulin 4200

corn starch 2100potato starch 1900wheat starch 1800

wood cellulose/lignocellulose ?????

Potential of JA-based bioethanol production

Jerusalem artichoke is easy to cultivate Relatively high production yields: 16–20

t/ha (even 20-80 t/ha) for tubers, and 18–28 t/ha green weight for foliage

High carbohydrate content: 15-20 % mainly inulin and fructo-oligosaccharides

Cultivate yearly Can be harvested like potato

Problems addressedSaccharomyces cerevisiae strain

does not exhibit so high inulinase activity

Treatment: acidic or enzymaticExogenous enzyme preparationLow efficiency of bioconversion (60-

70 %)

ObjectivesTechnological development for

enhancement of efficiency of ethanol production using

Jerusalem artichoke as raw material

Inoculation techniqueSimultan saccharification and fermentationSemi-continuous fermentation technology

Development of inoculation techniqueEffects of various inoculation

techniques were investigated

Best oneon initial day 2/3 amount of inoculum (cell number) and on second day: 1/3 amount

Efficiency of bioconversion: 90 %

SSF vs. Mixed Culture Fermentation

0 1 2 3 4 5 6 70.0

2.0

4.0

6.0

8.0

10.0

12.0

0.0

1.0

2.0

3.0

4.0

5.0

6.0

7.0

Saccharomyces cerevisiae+ inulinase

Total carbohydrates Sugars with higher DPSaccharose Fermentable sugarsEthanol

Fermentation time (day)

Car

bohy

drat

es (

w/v

%)

Etha

nol c

once

ntra

tion

v/v%

0 1 2 3 4 5 6 70.0

2.0

4.0

6.0

8.0

10.0

12.0

0.0

1.0

2.0

3.0

4.0

5.0

6.0

7.0

Mixed cultures (S. cerevisiae + Kl. marxianus)

Total carbohydrates Sugars with higher DPSaccharose Fermentable sugarsEthanol

Fermentation times (day)

Car

bohy

drat

es (

w/v

%)

Etha

nol c

once

ntra

tion

(v/v

%)

Efficiency of bioconverion: 85-88 %

Semi-continuous technology

0.00 4.00 8.00 12.00 16.00 20.00 24.00 28.00 32.00 36.00 40.00 44.000

2

4

6

8

10

12

14

16

0

1

2

3

4

5

6

7

Carbohydrates Ethanol

Fermentation time(days)

Etha

nol c

once

ntra

tion

(v/

v%)

Carb

ohyd

rate

s (w

/v%

)

Fresh mash

Harvested

Fresh mixed culture

ConclusionThe use of mixed culture in combination with semi-continuous fermentation technology definetely increases in the productivity, thus it is very promising for development of technology for production of bioethanol based on Jerusalem artichoke as raw material

AcknowledgementThis work was supported by

Hungarian Development Agency through Project number TÁMOP-4.2.1./B-09/1-KMR-2010-0005 and TECH_09-A3-2009-0194, as well as Hungarian Academy of Science through Bolyai János Research Grant

Thank you for your kind attention!