engineering e.coli to see light
DESCRIPTION
Engineering E.Coli to see Light. Voigt CA et. al. 2005. Light Engineering Imaging using E.Coli. Stimulus. Output. System. Sensor. Signal Transducer. Effector. Stimulus. Red Light Highly defined edges Two-dimensional image. Extracellular sensor. Intracellular regulator. Sensor. - PowerPoint PPT PresentationTRANSCRIPT
Engineering E.Coli to see Light
Voigt CA et. al. 2005
Light Engineering Imaging using E.Coli
SystemStimulus
SensorSensor
EffectorEffectorOutput
Signal Transducer
Signal Transducer
Stimulus
Red Light Highly defined edges Two-dimensional image
Sensor
Phytochrome Membrane bound extracellular sensor Intracellular regulator
Fusion protein: phytochrome and EnvZ histidine kinase domain
Extracellular sensor
Intracellular regulator
Photoreceptor Expression
PCB not produced in E.Coli Introduction of PCB synthesis genes Converts haem into PCB
Transduction Mechanism
Red light represses EnvZ auto-phosphorylation
Output
OmpR-P activates promoter linked to reporter gene
lacZ reporter produce black compound
OmpR-P dependent ompC promoter
System Revisited
Review
Created novel genetic circuit High definition film
(~100 megapixels per square inch) lacZ expression as a function of
light intensity / wavelength Transfer function
Something for the team to think about!!
Light Canon
Characterisation
Macro Resolution
Micro Transfer function SigB activity as a function of:
Intensity Wavelength